RESUMO
Background: The roots of Verbascum sinaiticum have been used traditionally for the management of wound in different regions of Ethiopia. Despite the presence of several claims and in vitro studies regarding its role in wound healing, no scientific studies have been conducted so far. Therefore, this study aims to scientifically evaluate the wound healing activities of the crude extract and solvent fractions of the roots of Verbascum sinaiticum in Swiss albino mice. Methods: The dried root powder of Verbascum sinaiticum was extracted using 80% methanol by maceration technique. This was then fractionated with chloroform, ethyl acetate, and water. These extracts were formulated as ointment at 5% and 10% concentration by using simple base. Acute dermal toxicity was performed on mice. The wound healing potential was evaluated using excision, incision, and burn wound models. Results: In excision wound, 10% and 5% of crude extract ointment provided a significant (P<0.001) percentage of contraction starting from day 4 and day 6 onwards respectively. Moreover, the rate of epithelialization was significantly (P<0.001) improved in 10% crude extract. In burn wound, 10% and 5% crude extract showed significant (P<0.001) wound contraction starting from day 4 and 8 onwards respectively. In both excision and burn wounds, a moderate concentration of fibroblast proliferation and collagen deposition was observed on the 10% crude extract. The 5% and 10% aqueous and ethyl acetate fractions produced a significant (P<0.001) percentage of wound contraction and shortening of epithelialization at different time points compared to simple ointment. Conclusion: The results of this study demonstrated that the 80% methanolic crude extract, aqueous and ethyl acetate fractions of Verbascum sinaiticum root have wound healing potential which assimilates its traditional use.
RESUMO
OBJECTIVES: People living with disability are more vulnerable to dental caries and have a high decayed, missed, and filled permanent teeth (DMFT) index and untreated dental disease than nondisabled individuals. In Ethiopia, there is a dearth of information on the oral health status of the disabled population. Hence, this study aimed to determine the prevalence of dental caries and its predictors among special needs school students in the Amhara region, Ethiopia. METHODS: An institution-based cross-sectional study was done on special needs school students in the Amhara region from November 2020 to April 2021. The study participants were recruited using a simple random sampling technique using a computer random generator. Data collection was done using the World Health Organization oral health survey tool. Data entry was done using Epi-data 4.6 and analyzed using SPSS 26. A logistic regression model was used to identify the possible predictors of dental caries. RESULTS: Four hundred and forty-three students with a mean age of 15.8 ± 3.8 were included in the study. The prevalence of dental caries was 41.5% (95% confidence interval [CI]: 36.3, 46.0) in permanent dentition with a mean DMFT score of 1.3 ± 1.6. The prevalence of dental caries in primary dentition was 23.1% (95% CI: 11.9, 32.1) with a mean decayed, missed, and filled primary teeth (dmft) score of 1.9 ± 0.2. Being 7-12 years old (adjusted odds ratios [AOR] = 3.6, 95% CI: 1.6, 8.3), lower grade level (AOR = 2.4, 95% CI:1.3,4.4), poor oral hygiene status (AOR = 2.5, 95% CI: 1.3, 4.8), and lack of parental support during tooth brushing (AOR = 2.2, 95% CI: 1.2, 4.1) were independent predictors of dental caries. CONCLUSIONS: A significant amount of special needs school students in the study area had dental caries. Age, grade level, oral hygiene status, and lack of parent support during tooth brushing were independent predictors of dental caries.