Real-time simultaneous refractive index and thickness mapping of sub-cellular biology at the diffraction limit.

Mapping the cellular refractive index (RI) is a central task for research involving the composition of microorganisms and the development of models providing automated medical screenings with accuracy beyond 95%. These models require significantly enhancing the state-of-the-art RI mapping capabilities to provide large amounts of accurate RI data at high throughput. Here, we present a machine-learning-based technique that obtains a biological specimen's real-time RI and thickness maps from a single image acquired with a conventional color camera. This technology leverages a suitably engineered nanostructured membrane that stretches a biological analyte over its surface and absorbs transmitted light, generating complex reflection spectra from each sample point. The technique does not need pre-existing sample knowledge. It achieves 10-4 RI sensitivity and sub-nanometer thickness resolution on diffraction-limited spatial areas. We illustrate practical application by performing sub-cellular segmentation of HCT-116 colorectal cancer cells, obtaining complete three-dimensional reconstruction of the cellular regions with a characteristic length of 30 µm. These results can facilitate the development of real-time label-free technologies for biomedical studies on microscopic multicellular dynamics.

Large-Scale and Wide-Gamut Coloration at the Diffraction Limit in Flexible, Self-Assembled Hierarchical Nanomaterials.

Unveiling physical phenomena that generate controllable structural coloration is at the center of significant research efforts due to the platform potential for the next generation of printing, sensing, displays, wearable optoelectronics components, and smart fabrics. Colors based on e-beam facilities possess high resolutions above 100k dots per inch (DPI), but limit manufacturing scales up to 4.37 cm2 , while requiring rigid substrates that are not flexible. State-of-art scalable techniques, on the contrary, provide either narrow gamuts or small resolutions. A common issue of current methods is also a heterogeneous resolution, which typically changes with the color printed. Here, a structural coloration platform with broad gamuts exceeding the red, green, and blue (RGB) spectrum in inexpensive, thermally resistant, flexible, and metallic-free structures at constant 101 600 DPI (at the diffraction limit), obtained via mass-production manufacturing is demonstrated. This platform exploits a previously unexplored physical mechanism, which leverages the interplay between strong scattering modes and optical resonances excited in fully 3D dielectric nanostructures with suitably engineered longitudinal profiles. The colors obtained with this technology are scalable to any area, demonstrated up to the single wafer (4 in.). These results open real-world applications of inexpensive, high-resolution, large-scale structural colors with broad chromatic spectra.