RESUMO
Hippocampal replay - the time-compressed, sequential reactivation of ensembles of neurons related to past experience - is a key neural mechanism of memory consolidation. Replay typically coincides with a characteristic pattern of local field potential activity, the sharp-wave ripple (SWR). Reduced SWR rates are associated with cognitive impairment in multiple models of neurodegenerative disease, suggesting that a clinically viable intervention to promote SWRs and replay would prove beneficial. We therefore developed a neurofeedback paradigm for rat subjects in which SWR detection triggered rapid positive feedback in the context of a memory-dependent task. This training protocol increased the prevalence of task-relevant replay during the targeted neurofeedback period by changing the temporal dynamics of SWR occurrence. This increase was also associated with neural and behavioral forms of compensation after the targeted period. These findings reveal short-timescale regulation of SWR generation and demonstrate that neurofeedback is an effective strategy for modulating hippocampal replay.
Assuntos
Hipocampo , Neurorretroalimentação , Animais , Ratos , Hipocampo/fisiologia , Masculino , Consolidação da Memória/fisiologia , Memória/fisiologia , Neurônios/fisiologiaRESUMO
Humans can remember specific events without acting on them and can influence which memories are retrieved based on internal goals. However, current animal models of memory typically present sensory cues to trigger retrieval and assess retrieval based on action 1-5 . As a result, it is difficult to determine whether measured patterns of neural activity relate to the cue(s), the retrieved memory, or the behavior. We therefore asked whether we could develop a paradigm to isolate retrieval-related neural activity in animals without retrieval cues or the requirement of a behavioral report. To do this, we focused on hippocampal "place cells." These cells primarily emit spiking patterns that represent the animal's current location (local representations), but they can also generate representations of previously visited locations distant from the animal's current location (remote representations) 6-13 . It is not known whether animals can deliberately engage specific remote representations, and if so, whether this engagement would occur during specific brain states. So, we used a closed-loop neurofeedback system to reward expression of remote representations that corresponded to uncued, experimenter-selected locations, and found that rats could increase the prevalence of these specific remote representations over time; thus, demonstrating memory retrieval modulated by internal goals in an animal model. These representations occurred predominately during periods of immobility but outside of hippocampal sharp-wave ripple (SWR) 13-15 events. This paradigm enables future direct studies of memory retrieval mechanisms in the healthy brain and in models of neurological disorders.
RESUMO
The hippocampus is a mammalian brain structure that expresses spatial representations1 and is crucial for navigation2,3. Navigation, in turn, intricately depends on locomotion; however, current accounts suggest a dissociation between hippocampal spatial representations and the details of locomotor processes. Specifically, the hippocampus is thought to represent mainly higher-order cognitive and locomotor variables such as position, speed and direction of movement4-7, whereas the limb movements that propel the animal can be computed and represented primarily in subcortical circuits, including the spinal cord, brainstem and cerebellum8-11. Whether hippocampal representations are actually decoupled from the detailed structure of locomotor processes remains unknown. To address this question, here we simultaneously monitored hippocampal spatial representations and ongoing limb movements underlying locomotion at fast timescales. We found that the forelimb stepping cycle in freely behaving rats is rhythmic and peaks at around 8 Hz during movement, matching the approximately 8 Hz modulation of hippocampal activity and spatial representations during locomotion12. We also discovered precisely timed coordination between the time at which the forelimbs touch the ground ('plant' times of the stepping cycle) and the hippocampal representation of space. Notably, plant times coincide with hippocampal representations that are closest to the actual position of the nose of the rat, whereas between these plant times, the hippocampal representation progresses towards possible future locations. This synchronization was specifically detectable when rats approached spatial decisions. Together, our results reveal a profound and dynamic coordination on a timescale of tens of milliseconds between central cognitive representations and peripheral motor processes. This coordination engages and disengages rapidly in association with cognitive demands and is well suited to support rapid information exchange between cognitive and sensory-motor circuits.
Assuntos
Hipocampo , Locomoção , Navegação Espacial , Animais , Ratos , Membro Anterior/fisiologia , Hipocampo/fisiologia , Locomoção/fisiologia , Navegação Espacial/fisiologia , Tomada de Decisões , Fatores de Tempo , Cognição/fisiologia , Vias EferentesRESUMO
Specific classes of GABAergic neurons play specific roles in regulating information processing in the brain. In the hippocampus, two major classes, parvalbumin-expressing (PV+) and somatostatin-expressing (SST+), differentially regulate endogenous firing patterns and target subcellular compartments of principal cells. How these classes regulate the flow of information throughout the hippocampus is poorly understood. We hypothesize that PV+ and SST+ interneurons in the dentate gyrus (DG) and CA3 differentially modulate CA3 patterns of output, thereby altering the influence of CA3 on CA1. We find that while suppressing either interneuron class increases DG and CA3 output, the effects on CA1 were very different. Suppressing PV+ interneurons increases local field potential signatures of coupling from CA3 to CA1 and decreases signatures of coupling from entorhinal cortex to CA1; suppressing SST+ interneurons has the opposite effect. Thus, DG and CA3 PV+ and SST+ interneurons bidirectionally modulate the flow of information through the hippocampal circuit.
Assuntos
Região CA1 Hipocampal/fisiologia , Região CA3 Hipocampal/fisiologia , Giro Denteado/fisiologia , Córtex Entorrinal/fisiologia , Neurônios GABAérgicos/fisiologia , Interneurônios/fisiologia , Somatostatina/metabolismo , Potenciais de Ação , Animais , Região CA1 Hipocampal/citologia , Região CA3 Hipocampal/citologia , Giro Denteado/citologia , Córtex Entorrinal/citologia , Feminino , Neurônios GABAérgicos/citologia , Interneurônios/citologia , Masculino , Camundongos , Camundongos Endogâmicos C57BLRESUMO
Representations related to past experiences play a critical role in memory and decision-making processes. The rat hippocampus expresses these types of representations during sharp-wave ripple (SWR) events, and previous work identified a minority of SWRs that contain 'replay' of spatial trajectories at â¼20x the movement speed of the animal. Efforts to understand replay typically make multiple assumptions about which events to examine and what sorts of representations constitute replay. We therefore lack a clear understanding of both the prevalence and the range of representational dynamics associated with replay. Here, we develop a state space model that uses a combination of movement dynamics of different speeds to capture the spatial content and time evolution of replay during SWRs. Using this model, we find that the large majority of replay events contain spatially coherent, interpretable content. Furthermore, many events progress at real-world, rather than accelerated, movement speeds, consistent with actual experiences.
Assuntos
Hipocampo/fisiologia , Consolidação da Memória , Potenciais de Ação , Animais , Comportamento Animal , Masculino , Memória , Modelos Neurológicos , Ratos , Ratos Long-EvansRESUMO
Executing memory-guided behavior requires storage of information about experience and later recall of that information to inform choices. Awake hippocampal replay, when hippocampal neural ensembles briefly reactivate a representation related to prior experience, has been proposed to critically contribute to these memory-related processes. However, it remains unclear whether awake replay contributes to memory function by promoting the storage of past experiences, facilitating planning based on evaluation of those experiences, or both. We designed a dynamic spatial task that promotes replay before a memory-based choice and assessed how the content of replay related to past and future behavior. We found that replay content was decoupled from subsequent choice and instead was enriched for representations of previously rewarded locations and places that had not been visited recently, indicating a role in memory storage rather than in directly guiding subsequent behavior.
Assuntos
Comportamento de Escolha/fisiologia , Hipocampo/fisiologia , Memória/fisiologia , Percepção Espacial/fisiologia , Algoritmos , Animais , Condicionamento Operante , Eletrodos Implantados , Objetivos , Modelos Lineares , Masculino , Aprendizagem em Labirinto , Ratos , Ratos Long-EvansRESUMO
Animal behavior provides context for understanding disease models and physiology. However, that behavior is often characterized subjectively, creating opportunity for misinterpretation and misunderstanding. For example, spatial alternation tasks are treated as paradigmatic tools for examining memory; however, that link is actually an assumption. To test this assumption, we simulated a reinforcement learning (RL) agent equipped with a perfect memory process. We found that it learns a simple spatial alternation task more slowly and makes different errors than a group of male rats, illustrating that memory alone may not be sufficient to capture the behavior. We demonstrate that incorporating spatial biases permits rapid learning and enables the model to fit rodent behavior accurately. Our results suggest that even simple spatial alternation behaviors reflect multiple cognitive processes that need to be taken into account when studying animal behavior.SIGNIFICANCE STATEMENT Memory is a critical function for cognition whose impairment has significant clinical consequences. Experimental systems aimed at testing various sorts of memory are therefore also central. However, experimental designs to test memory are typically based on intuition about the underlying processes. We tested this using a popular behavioral paradigm: a spatial alternation task. Using behavioral modeling, we show that the straightforward intuition that these tasks just probe spatial memory fails to account for the speed at which rats learn or the types of errors they make. Only when memory-independent dynamic spatial preferences are added can the model learn like the rats. This highlights the importance of respecting the complexity of animal behavior to interpret neural function and validate disease models.
Assuntos
Modelos Neurológicos , Aprendizagem Espacial , Memória Espacial , Animais , Encéfalo/fisiologia , Intuição , Masculino , Ratos , Ratos Long-Evans , RecompensaRESUMO
Alzheimer's disease (AD) is characterized by progressive memory loss, and there is a pressing need to identify early pathophysiological alterations that predict subsequent memory impairment. Hippocampal sharp-wave ripples (SWRs)-electrophysiological signatures of memory reactivation in the hippocampus-are a compelling candidate for this purpose. Mouse models of AD show reductions in both SWR abundance and associated slow gamma (SG) power during aging, but these alterations have yet to be directly linked to memory impairments. In aged apolipoprotein E4 knockin (apoE4-KI) mice-a model of the major genetic risk factor for AD-we find that reduced SWR abundance and associated CA3 SG power predicted spatial memory impairments measured 1-2 months later. Importantly, SWR-associated CA3 SG power reduction in young apoE4-KI mice also predicted spatial memory deficits measured 10 months later. These results establish features of SWRs as potential functional biomarkers of memory impairment in AD.
Assuntos
Doença de Alzheimer/metabolismo , Hipocampo/metabolismo , Transtornos da Memória/metabolismo , Doença de Alzheimer/patologia , Animais , Apolipoproteína E4/metabolismo , Biomarcadores/metabolismo , Região CA3 Hipocampal/metabolismo , Região CA3 Hipocampal/patologia , Modelos Animais de Doenças , Hipocampo/patologia , Aprendizagem/fisiologia , Transtornos da Memória/patologia , Camundongos , Fatores de Risco , Memória Espacial/fisiologiaRESUMO
New recording technologies and the potential for closed-loop experiments have led to an increasing demand for computationally efficient and accurate algorithms to decode population spiking activity in multi-dimensional spaces. Exact point process filters can accurately decode low-dimensional signals, but are computationally intractable for high-dimensional signals. Approximate Gaussian filters are computationally efficient, but are inaccurate when the signals have complex distributions and nonlinear dynamics. Even particle filter methods tend to become inefficient and inaccurate when the filter distribution has multiple peaks. Here, we develop a new point process filter algorithm that combines the computational efficiency of approximate Gaussian methods with a numerical accuracy that exceeds standard particle filters. We use a mixture of Gaussian model for the posterior at each time step, allowing for an analytic solution to the computationally expensive filter integration step. During non-spike intervals, the filter needs only to update the mean, covariance, and mixture weight of each component. At spike times, a sampling procedure is used to update the filtering distribution and find the number of Gaussian mixture components necessary to maintain an accurate approximation. We illustrate the application of this algorithm to the problem of decoding a rat's position and velocity in a maze from hippocampal place cell data using both 2-D and 4-D decoders.
Assuntos
Potenciais de Ação/fisiologia , Modelos Neurológicos , Processamento de Sinais Assistido por Computador , Algoritmos , Animais , Eletrofisiologia , Hipocampo/fisiologia , Aprendizagem em Labirinto/fisiologia , Neurônios/fisiologia , Distribuição Normal , RatosRESUMO
The emergence of deep learning techniques has provided new tools for the analysis of complex data in the field of neuroscience. In parallel, advanced statistical approaches like point-process modeling provide powerful tools for analyzing the spiking activity of neural populations. How statistical and machine learning techniques compare when applied to neural data remains largely unclear. In this research, we compare the performance of a point-process filter and a long short-term memory (LSTM) network in decoding the 2D movement trajectory of a rat using the neural activity recorded from an ensemble of hippocampal place cells. We compute the least absolute error (LAE), a measure of accuracy of prediction, and the coefficient of determination (R2), a measure of prediction consistency, to compare the performance of these two methods. We show that the LSTM and point-process filter provide comparable accuracy in predicting the position; however, the point-process provides further information about the prediction which is unavailable for LSTM. Though previous results report better performance using deep learning techniques, our results indicate that this is not universally the case. We also investigate how these techniques encode information carried by place cell activity and compare the computational efficiency of the two methods. While the point-process model is built using the receptive field for each place cell, we show that LSTM does not necessarily encode receptive fields, but instead decodes the movement trajectory using other features of neural activity. Although it is less robust, LSTM runs more than 7 times faster than the fastest point-process filter in this research, providing a strong advantage in computational efficiency. Together, these results suggest that the point-process filters and LSTM approaches each provide distinct advantages; the choice of model should be informed by the specific scientific question of interest.
Assuntos
Aprendizado Profundo , Células de Lugar , Animais , Movimento , RatosRESUMO
The hippocampus is well known as a central site for memory processing-critical for storing and later retrieving the experiences events of daily life so they can be used to shape future behavior. Much of what we know about the physiology underlying hippocampal function comes from spatial navigation studies in rodents, which have allowed great strides in understanding how the hippocampus represents experience at the cellular level. However, it remains a challenge to reconcile our knowledge of spatial encoding in the hippocampus with its demonstrated role in memory-dependent tasks in both humans and other animals. Moreover, our understanding of how networks of neurons coordinate their activity within and across hippocampal subregions to enable the encoding, consolidation, and retrieval of memories is incomplete. In this chapter, we explore how information may be represented at the cellular level and processed via coordinated patterns of activity throughout the subregions of the hippocampal network.
Assuntos
Hipocampo/fisiologia , Aprendizagem/fisiologia , Neurônios/fisiologia , Comportamento Espacial/fisiologia , Animais , Humanos , Rede Nervosa/fisiologia , Vias Neurais/fisiologiaRESUMO
Apolipoprotein (apo) E4 is the major genetic risk factor for Alzheimer's disease (AD), but the mechanism by which it causes cognitive decline is unclear. In knockin (KI) mice, human apoE4 causes age-dependent learning and memory impairments and degeneration of GABAergic interneurons in the hippocampal dentate gyrus. Here we report two functional apoE4-KI phenotypes involving sharp-wave ripples (SWRs), hippocampal network events critical for memory processes. Aged apoE4-KI mice had fewer SWRs than apoE3-KI mice and significantly reduced slow gamma activity during SWRs. Elimination of apoE4 in GABAergic interneurons, which prevents learning and memory impairments, rescued SWR-associated slow gamma activity but not SWR abundance in aged mice. SWR abundance was reduced similarly in young and aged apoE4-KI mice; however, the full SWR-associated slow gamma deficit emerged only in aged apoE4-KI mice. These results suggest that progressive decline of interneuron-enabled slow gamma activity during SWRs critically contributes to apoE4-mediated learning and memory impairments. VIDEO ABSTRACT.
Assuntos
Apolipoproteína E4/metabolismo , Transtornos Cognitivos/metabolismo , Hipocampo/metabolismo , Interneurônios/metabolismo , Transtornos da Memória/metabolismo , Envelhecimento , Doença de Alzheimer/genética , Doença de Alzheimer/metabolismo , Animais , Apolipoproteína E4/genética , Transtornos Cognitivos/genética , Modelos Animais de Doenças , Técnicas de Introdução de Genes/métodos , Aprendizagem em Labirinto/fisiologia , Transtornos da Memória/genética , Camundongos TransgênicosRESUMO
Apolipoprotein (apo) E4 is expressed in many types of brain cells, is associated with age-dependent decline of learning and memory in humans, and is the major genetic risk factor for AD. To determine whether the detrimental effects of apoE4 depend on its cellular sources, we generated human apoE knock-in mouse models in which the human APOE gene is conditionally deleted in astrocytes, neurons, or GABAergic interneurons. Here we report that deletion of apoE4 in astrocytes does not protect aged mice from apoE4-induced GABAergic interneuron loss and learning and memory deficits. In contrast, deletion of apoE4 in neurons does protect aged mice from both deficits. Furthermore, deletion of apoE4 in GABAergic interneurons is sufficient to gain similar protection. This study demonstrates a detrimental effect of endogenously produced apoE4 on GABAergic interneurons that leads to learning and memory deficits in mice and provides a novel target for drug development for AD related to apoE4.
Assuntos
Apolipoproteína E4/biossíntese , Neurônios GABAérgicos/metabolismo , Interneurônios/metabolismo , Aprendizagem/fisiologia , Transtornos da Memória/metabolismo , Animais , Feminino , Neurônios GABAérgicos/patologia , Humanos , Interneurônios/patologia , Transtornos da Memória/patologia , Camundongos , Camundongos TransgênicosRESUMO
Excitatory and inhibitory balance of neuronal network activity is essential for normal brain function and may be of particular importance to memory. Apolipoprotein (apo) E4 and amyloid-ß (Aß) peptides, two major players in Alzheimer's disease (AD), cause inhibitory interneuron impairments and aberrant neuronal activity in the hippocampal dentate gyrus in AD-related mouse models and humans, leading to learning and memory deficits. To determine whether replacing the lost or impaired interneurons rescues neuronal signaling and behavioral deficits, we transplanted embryonic interneuron progenitors into the hippocampal hilus of aged apoE4 knock-in mice without or with Aß accumulation. In both conditions, the transplanted cells developed into mature interneurons, functionally integrated into the hippocampal circuitry, and restored normal learning and memory. Thus, restricted hilar transplantation of inhibitory interneurons restores normal cognitive function in two widely used AD-related mouse models, highlighting the importance of interneuron impairments in AD pathogenesis and the potential of cell replacement therapy for AD. More broadly, it demonstrates that excitatory and inhibitory balance are crucial for learning and memory, and suggests an avenue for investigating the processes of learning and memory and their alterations in healthy aging and diseases.
Assuntos
Doença de Alzheimer , Apolipoproteína E4/genética , Hipocampo/patologia , Interneurônios/fisiologia , Aprendizagem/fisiologia , Memória/fisiologia , Células-Tronco Neurais/transplante , Doença de Alzheimer/genética , Doença de Alzheimer/fisiopatologia , Doença de Alzheimer/cirurgia , Precursor de Proteína beta-Amiloide/genética , Animais , Modelos Animais de Doenças , Feminino , Humanos , Técnicas In Vitro , Masculino , Aprendizagem em Labirinto , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Mutação/genética , Proteínas do Tecido Nervoso/metabolismoRESUMO
Cancer-associated fibroblasts (CAF) have been reported to support tumor progression by a variety of mechanisms. However, their role in the progression of non-small cell lung cancer (NSCLC) remains poorly defined. In addition, the extent to which specific proteins secreted by CAFs contribute directly to tumor growth is unclear. To study the role of CAFs in NSCLCs, a cross-species functional characterization of mouse and human lung CAFs was conducted. CAFs supported the growth of lung cancer cells in vivo by secretion of soluble factors that directly stimulate the growth of tumor cells. Gene expression analysis comparing normal mouse lung fibroblasts and mouse lung CAFs identified multiple genes that correlate with the CAF phenotype. A gene signature of secreted genes upregulated in CAFs was an independent marker of poor survival in patients with NSCLC. This secreted gene signature was upregulated in normal lung fibroblasts after long-term exposure to tumor cells, showing that lung fibroblasts are "educated" by tumor cells to acquire a CAF-like phenotype. Functional studies identified important roles for CLCF1-CNTFR and interleukin (IL)-6-IL-6R signaling in promoting growth of NSCLCs. This study identifies novel soluble factors contributing to the CAF protumorigenic phenotype in NSCLCs and suggests new avenues for the development of therapeutic strategies.
Assuntos
Carcinoma Pulmonar de Células não Pequenas/imunologia , Carcinoma Pulmonar de Células não Pequenas/patologia , Fibroblastos/imunologia , Fibroblastos/patologia , Interleucina-6/imunologia , Neoplasias Pulmonares/imunologia , Neoplasias Pulmonares/patologia , Adenocarcinoma/imunologia , Adenocarcinoma/patologia , Animais , Processos de Crescimento Celular/fisiologia , Linhagem Celular Tumoral , Humanos , Camundongos , Especificidade da Espécie , Células Estromais/imunologia , Células Estromais/patologia , Transplante HeterólogoRESUMO
BACKGROUND: Although extensive research has demonstrated the importance of excitatory granule neurons in the dentate gyrus of the hippocampus in normal learning and memory and in the pathogenesis of amnesia in Alzheimer's disease (AD), the role of hilar GABAergic inhibitory interneurons, which control the granule neuron activity, remains unclear. METHODOLOGY AND PRINCIPAL FINDINGS: We explored the function of hilar GABAergic interneurons in spatial learning and memory by inhibiting their activity through Cre-dependent viral expression of enhanced halorhodopsin (eNpHR3.0)--a light-driven chloride pump. Hilar GABAergic interneuron-specific expression of eNpHR3.0 was achieved by bilaterally injecting adeno-associated virus containing a double-floxed inverted open-reading frame encoding eNpHR3.0 into the hilus of the dentate gyrus of mice expressing Cre recombinase under the control of an enhancer specific for GABAergic interneurons. In vitro and in vivo illumination with a yellow laser elicited inhibition of hilar GABAergic interneurons and consequent activation of dentate granule neurons, without affecting pyramidal neurons in the CA3 and CA1 regions of the hippocampus. We found that optogenetic inhibition of hilar GABAergic interneuron activity impaired spatial learning and memory retrieval, without affecting memory retention, as determined in the Morris water maze test. Importantly, optogenetic inhibition of hilar GABAergic interneuron activity did not alter short-term working memory, motor coordination, or exploratory activity. CONCLUSIONS AND SIGNIFICANCE: Our findings establish a critical role for hilar GABAergic interneuron activity in controlling spatial learning and memory retrieval and provide evidence for the potential contribution of GABAergic interneuron impairment to the pathogenesis of amnesia in AD.
Assuntos
Giro Denteado/metabolismo , Neurônios GABAérgicos/metabolismo , Interneurônios/metabolismo , Aprendizagem/fisiologia , Memória/fisiologia , Animais , Giro Denteado/citologia , Dependovirus/genética , Genes Reporter , Vetores Genéticos/administração & dosagem , Vetores Genéticos/genética , Memória de Curto Prazo/fisiologia , Camundongos , Camundongos Transgênicos , Desempenho PsicomotorRESUMO
Apolipoprotein E4 (apoE4) plays a major role in the pathogenesis of Alzheimer's disease. Brain amyloid-ß (Aß) accumulation depends on age and apoE isoforms (apoE4 > apoE3) both in humans and in transgenic mouse models. Brain apoE levels are also isoform dependent, but in the opposite direction (apoE4 < apoE3). Thus, one prevailing hypothesis is to increase brain apoE expression to reduce Aß levels. To test this hypothesis, we generated mutant human amyloid precursor protein transgenic mice expressing one or two copies of the human APOE3 or APOE4 gene that was knocked in and flanked by LoxP sites. We report that reducing apoE3 or apoE4 expression by 50% in 6-month-old mice results in efficient Aß clearance and does not increase Aß accumulation. However, 12-month-old mice with one copy of the human APOE gene had significantly reduced Aß levels and plaque loads compared with mice with two copies, regardless of which human apoE isoform was expressed, suggesting a gene dose-dependent effect of apoE on Aß accumulation in aged mice. Additionally, 12-month-old mice expressing one or two copies of the human APOE4 gene had significantly higher levels of Aß accumulation and plaque loads than age-matched mice expressing one or two copies of the human APOE3 gene, suggesting an isoform-dependent effect of apoE on Aß accumulation in aged mice. Moreover, Cre-mediated APOE4 gene excision in hippocampal astrocytes significantly reduced insoluble Aß in adult mice. Thus, reducing, rather than increasing, apoE expression is an attractive approach to lowering brain Aß levels.
Assuntos
Envelhecimento/genética , Precursor de Proteína beta-Amiloide/genética , Apolipoproteína E3/deficiência , Apolipoproteína E4/deficiência , Apolipoproteínas E/deficiência , Mutação/genética , Envelhecimento/fisiologia , Precursor de Proteína beta-Amiloide/metabolismo , Animais , Apolipoproteína E3/genética , Apolipoproteína E4/genética , Apolipoproteínas E/antagonistas & inibidores , Apolipoproteínas E/fisiologia , Feminino , Técnicas de Introdução de Genes , Humanos , Masculino , Camundongos , Camundongos da Linhagem 129 , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos TransgênicosRESUMO
KRAS is one of the most frequently mutated human oncogenes. In some settings, oncogenic KRAS can trigger cellular senescence, whereas in others it produces hyperproliferation. Elucidating the mechanisms regulating these 2 drastically distinct outcomes would help identify novel therapeutic approaches in RAS-driven cancers. Using a combination of functional genomics and mouse genetics, we identified a role for the transcription factor Wilms tumor 1 (WT1) as a critical regulator of senescence and proliferation downstream of oncogenic KRAS signaling. Deletion or suppression of Wt1 led to senescence of mouse primary cells expressing physiological levels of oncogenic Kras but had no effect on wild-type cells, and Wt1 loss decreased tumor burden in a mouse model of Kras-driven lung cancer. In human lung cancer cell lines dependent on oncogenic KRAS, WT1 loss decreased proliferation and induced senescence. Furthermore, WT1 inactivation defined a gene expression signature that was prognostic of survival only in lung cancer patients exhibiting evidence of oncogenic KRAS activation. These findings reveal an unexpected role for WT1 as a key regulator of the genetic network of oncogenic KRAS and provide important insight into the mechanisms that regulate proliferation or senescence in response to oncogenic signals.