Effect of Leukoreduced Platelet Rich Plasma on Intra-Articular Pro-Inflammatory Cytokines in a Canine Pilot Study.

Evaluation of effect of Leukoreduced Platelet Rich Plasma (LrPRP) on TNF-α and IL-6 (pro-inflammatory cytokines) in joint fluid in dogs with cranial cruciate ligament rupture (CCLR). Eight client-owned dogs with CCLR were assigned to treatment (2 mL LrPRP) or control (2 mL saline) injection groups. Day of evaluation (day 0) and day of surgery (day 10-14), joint fluid was collected and joint injected. Joint fluid was also collected on day of suture removal (day 20-28). TNF-α and IL-6 concentrations of joint fluid were measured using a bead-based antibody assay. Concentrations at the later time points were expressed as a ratio to the initial level within each stifle. LrPRP had a mean concentration of platelets 1.7 times higher that of whole blood on day of evaluation and 1.4 times higher on day of the surgery. Leukocytes were reduced by 99.7%. On day of surgery, TNF-α ratios in the joint fluid from dogs injected with LrPRP were significantly different that TNF-α ratios of control group. On the day of suture removal ratios of IL-6 and TNF-α were lower in LrPRP treatment group compared with control group; however, differences were not significant. LrPRP modulate ratios of pro-inflammatory cytokine TNF-α in dogs with CCLR.

Accuracy of a drilling with a custom 3D printed guide or free-hand technique in canine experimental sacroiliac luxations.

OBJECTIVE: To improve the accuracy of drilling during the repair of sacroiliac luxations (SILs) with a 3D-printed patient-specific drill guide (3D-GDT) compared to free-hand drilling technique (FHDT). STUDY DESIGN: Blinded, randomized, prospective ex vivo study. SAMPLE POPULATION: Sixteen canine cadavers (20-25 kg). METHODS: Dorsal, bilateral SILs were created. Pelvic CT was performed pre- and post-drilling. The FHDT was drilled followed by 3D-GDT. CT and 3D measurements of craniocaudal and dorsoventral angles were compared between FHDT and 3D-GDT, as well as deviations of entry and exit points relative to optimal trajectory. RESULTS: Mean craniocaudal and dorsoventral angles for both CT- and 3D-measured 3D-GDT (CT 4.2 ± 3.9° and 3.9 ± 3.2°, respectively; 3D 5.1 ± 5.1° and 2.8 ± 2.3°, respectively p = .0006) were lower compared to FHDT (CT 11.8 ± 4.0°, p < .0001 and 8.9 ± 6.1°, p = .01; 3D 12.4 ± 5.9°, p = .0006 and 5.3 ± 5.24°, p = .05). Entry dorsoventral and end craniocaudal, dorsoventral, and 3D linear deviations were reduced with 3D-GDT. Sacral corridor disruption was present in 20% (3/15) for FHDT compared with 0% for 3D-GDT. CT and 3D analyses were in strong agreement (r = 0.77). CONCLUSION: Deviations of drill trajectories were minimized relative to optimal trajectories with 3D-GDT compared to FHDT in the dorsoventral and craniocaudal planes. CLINICAL SIGNIFICANCE: The use of 3D-GDT improves accuracy of sacral drilling compared with FHDT in canine cadavers. These results justify further evaluation in a clinical, prospective study.

Effect of Sedation on the Neurological Examination of the Patellar and Withdrawal Reflexes in Healthy Dogs.

Introduction: Pain, temperament, fear, and anxiety can prevent safe and accurate evaluation of common neurologic reflexes in dogs. When sedation is used it is unknown how the neurological examination, and specifically patellar and withdrawal reflexes are affected, and, if present, how long any effect might last. The purpose of this study is to investigate the effect of sedation on the evaluation of select common limb spinal reflexes in healthy dogs. Material and Methods: Fourteen healthy dogs with normal neurologic exams were included. After placing joint landmarks, patellar reflex and pelvic and thoracic limb withdrawal reflexes were tested. Joint angles were measured, obtaining reflex angle endpoints, change in angle, and change in time to reflex completion. These measurements were recorded at different time points: prior to sedation (awake timepoint), 15 and 30 min following administration of standardized sedation protocol of dexmedetomidine and butorphanol, and 15 and 30 min following administration of a standardized reversal agent, atipamazole. Results: For patellar reflex, the stifle end angle increased from 91.5 to 108.55 degrees (p < 0.0001) 15 min following sedation, and remained increased at 104.5 degrees (p < 0.0001) 30 min following sedation. Stifle change in angle increased from 9.6 to 24.4 degrees (p < 0.0001) 15 min following sedation, and remained increased at 20.85 degrees (p < 0.0001) and 11 degrees (p = 0.012) at 30 min sedation and 15 min reversal. Tarsal joint in pelvic withdrawal and elbow in thoracic withdrawal reflexes did not differ in at any timepoint of sedation or reversal when compared with the awake timepoint, for end angle or change in angle. The increases in end angle and change in angle for patellar reflex generated a change in time for patellar reflex from 0.12 s (awake) to 0.129 s (15 min sedation) which was statistically significant (p = 0.041). Change in time did not differ for pelvic withdrawal or thoracic withdrawal. Discussion/Conclusions: Reflexes were elicited in all dogs under sedation. Sedation does not affect the evaluation of the withdrawal reflex on any limb but improves the visualization of the patellar reflex in this group of neurologically normal dogs.

Effect of sedation or general anesthesia on elbow goniometry and thoracic limb circumference measurements in dogs with naturally occurring elbow osteoarthritis.

OBJECTIVE: To evaluate the effect of sedation or general anesthesia (GA) on elbow goniometry and thoracic limb circumference (TLC) measurements in dogs with elbow osteoarthritis (OA). STUDY DESIGN: Prospective study. ANIMALS: Twenty-four client-owned dogs with radiographically confirmed elbow OA. METHODS: Elbow goniometry and TLC measurements were made before and after either sedation or GA by using a hand-held goniometer and spring tension measuring tape, respectively. Observers were not allowed to review their pre-sedation or pre-GA measurements at the time of obtaining measurements on dogs under sedation or GA. Mixed analysis of variance models were used to compare elbow goniometry and TLC measurements before and after sedation or GA. RESULTS: Eleven and thirteen dogs were included in the sedation and GA groups, respectively. Mean elbow flexion decreased by 5° and 3° and mean elbow extension increased by 6° and 2° under sedation and GA, respectively. Total range of motion increased by 11° under sedation and by 5° under GA. Each of these changes was statistically significant (P < .05) except elbow extension under GA (P = .129). Sedation and GA did not influence TLC measurements (P > .05). CONCLUSION: Sedation or GA led to slight and similar increase in elbow flexion and extension but did not influence TLC measurements in dogs with elbow OA. CLINICAL SIGNIFICANCE: Sedation or GA can cause slight alterations to goniometric measurements in canine elbows with OA. The protocols used in this study for sedation and GA seem interchangeably acceptable for goniometry and TLC measurements in dogs with elbow OA.

Morphometric measurements to predict meniscal size in skeletally mature dogs for meniscal transplantation.

OBJECTIVE: To determine a relationship between morphometric measurements (MM) and meniscal dimensions (MD) in the dog. STUDY DESIGN: Cadaveric study. ANIMALS: Eighteen mixed-breed canine cadavers (22.35 ± 4.06 kg); 36 each of medial and lateral menisci. METHODS: The following MM were obtained in duplicate: height at scapula, withers, pelvis, rump, and greater trochanter; chest circumference; pelvic circumference; length from occiput to the base of the tail; hock to stifle length (HS); body weight; body condition score; and body fat percentage (BF%). Stifles were disarticulated, and digital photographs of in situ menisci were used to obtain meniscal measurements in duplicate. Morphometric parameters were compared with MD via Pearson correlation (r). A correlation of r ≥ 0.65 was considered strong. RESULTS: The strongest correlation was noted between HS and MD, with r = 0.85 for lateral meniscal width, r = 0.77 for medial meniscal length, and r = 0.76 for medial meniscal width. Lateral meniscal length had the strongest correlation with height at rump (HRu) (r = 0.73). Body weight correlated strongly with meniscal width and fairly with meniscal length. Body condition score and BF% correlated weakly with MD. CONCLUSION: Hock to stifle length was an easily obtainable variable and was proportional to MD. CLINICAL SIGNIFICANCE: Hock to stifle length may be considered by tissue banks and veterinary surgeons as a quick and cost effective screening tool for appropriate meniscal sizing in dogs.

Effect of tibial rotation on the tibial plateau angle measurement in dogs: An ex vivo study.

OBJECTIVE: To determine the effect of tibial rotation on radiographic tibial plateau angle (TPA) measurement in normal and cranial cruciate ligament (CrCL)-deficient stifles. STUDY DESIGN: Cadaveric study. SAMPLE POPULATION: Five pairs of canine cadaver hind limbs with no stifle osteoarthritis. METHODS: Mediolateral radiographs of each specimen were obtained at 0°, 10°, 20°, and 30° of internal and external rotation of the tibia with both intact and transected CrCL. Two Kirschner wires were placed at the cranial and caudal landmarks of the tibial plateau, and the 0° views were imaged again, representing the anatomic TPA. Five observers measured the TPA twice on all images. Measurements were compared by using a mixed-model ANOVA. Intraobserver and interobserver variability were evaluated by using the coefficient of variation. RESULTS: Tibial plateau angle did not differ among the anatomic, CrCL-intact, and CrCL-deficient 0° views or between the CrCL-intact and CrCL-deficient views at the various tibial rotations. The only difference detected was between CrCL-intact stifles radiographed at 0° and external rotation (range, 1.16°-1.6°; P = .016, P = .001, and P < .001). Tibial rotation, CrCL transection, and their interaction all failed to influence intraobserver and interobserver variability (P > .05). CONCLUSION: In CrCL-deficient stifles, tibial rotation of up to 30° internally and externally did not result in clinically relevant changes in TPA measurements. CLINICAL SIGNIFICANCE: The TPA can be successfully measured with up to 30° of internal and external tibial rotation in CrCL-deficient stifles with no osteoarthritic changes.

Efficacy of adhesive incise drapes in preventing bacterial contamination of clean canine surgical wounds.

OBJECTIVE: To investigate the efficacy of adhesive incise drapes in reducing bacterial contamination of clean canine surgical wounds. STUDY DESIGN: Randomized clinical trial. ANIMALS: Dogs (n=100) having elective ovariohysterectomy or stifle surgery. METHODS: Dogs were randomly assigned to 1 of 2 groups: drape or no drape. Swabs obtained from the inner edge of the surgical wound at the beginning (swab 1) and end (swab 2) of surgery were submitted for microbial culture. Number of colony forming units was counted for all positive cultures and change in bacterial counts between swabs 1 and 2 was calculated. Percentage adhesive drape adherence at the end of surgery was calculated from a digital photograph of the surgical site. duration of surgery/anesthesia and the anesthetic induction agent used were recorded. RESULTS: There was a significant increase in bacterial counts between swabs 1 and 2 (P=.001). Wound contamination was 14% (6 drape; 8 no drape; P=0.78) with Staphylococcus spp. most commonly isolated. Median percentage drape adherence at the end of surgery was 89.3% (0-100%). Duration of anesthesia was significantly related to wound contamination (P=.013), but duration of surgery and anesthesia induction agent were not. CONCLUSIONS: Adhesive incise drapes did not reduce wound contamination of clean canine surgical wounds. CLINICAL RELEVANCE: Use of adhesive incise drapes in clean surgical procedures is of questionable benefit in dogs.