RESUMO
Sporothrix schenkii is a dimorphic fungus that causes infections in both humans and animals. We report on 25 S. schenkii isolates collected in 2017 from humans and cats clinically diagnosed with sporotrichosis, in Malaysia. These isolates were phenotypically identified as S. schenkii sensu lato and further defined as S. schenckii sensu stricto based on partial calmodulin gene sequence. Isolates from both humans and cats were genotypically identical but displayed phenotypic variation. Phylogenetic analyses based on partial calmodulin sequence showed that the Malaysian isolates clustered with global S. schenkii sensu stricto strains, in particular, of the AFLP type E. This analysis also revealed that partial calmodulin sequence alone was sufficient for classifying global S. schenckii sensu stricto strains into their respective AFLP types, from A to E. The genetically conserved S. schenkii sensu stricto species isolated from humans and cats is suggestive of a clonal strain present in Malaysia. To the best of our knowledge, this is the first report on molecular identification of Sporothrix schenkii strains from human infections in Malaysia. Further studies are required in order to elucidate the clonal nature of Malaysian S. schenkii isolates. Our findings indicate the presence of a predominant S. schenkii genotype in the environment, causing infections in both cats and humans in Malaysia.
Assuntos
Doenças do Gato/epidemiologia , Doenças do Gato/microbiologia , Sporothrix/classificação , Sporothrix/isolamento & purificação , Esporotricose/epidemiologia , Esporotricose/microbiologia , Adolescente , Adulto , Idoso , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Animais , Calmodulina/genética , Gatos , Criança , Humanos , Malásia/epidemiologia , Pessoa de Meia-Idade , Epidemiologia Molecular , Filogenia , Reação em Cadeia da Polimerase , Sporothrix/genética , Esporotricose/veterinária , Adulto JovemRESUMO
Candida pseudohaemulonii is phylogenetically close to the C. haemulonii complex and exhibits resistance to amphotericin B and azole agents. We report here the draft genome sequence of C. pseudohaemulonii UZ153_17 isolated from the blood culture of a neutropenic patient. The draft genome is 3,532,003,666 bp in length, with 579,838 reads, 130 contigs, and a G+C content of 47.15%.
RESUMO
We report a fatal case of Candida auris that was involved in mixed candidemia with Candida tropicalis, isolated from the blood of a neutropenic patient. Identification of both isolates was confirmed by amplification and sequencing of internal transcribed spacer and D1/D2 domain of large subunit in rRNA gene. Antifungal susceptibility test by E-test method revealed that C. auris was resistant to amphotericin B, anidulafungin, caspofungin, fluconazole, itraconazole and voriconazole. On the other hand, C. tropicalis was sensitive to all antifungal tested. The use of chromogenic agar as isolation media is vital in detecting mixed candidemia.
Assuntos
Candida/classificação , Candida/isolamento & purificação , Candidemia/diagnóstico , Candidemia/patologia , Coinfecção/diagnóstico , Coinfecção/patologia , Neutropenia/complicações , Antifúngicos/farmacologia , Candida/efeitos dos fármacos , Candida/genética , Candidemia/microbiologia , Coinfecção/microbiologia , Meios de Cultura/química , DNA Fúngico/química , DNA Fúngico/genética , DNA Ribossômico/química , DNA Ribossômico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Farmacorresistência Fúngica Múltipla , Evolução Fatal , Humanos , Masculino , Pessoa de Meia-Idade , RNA Ribossômico/genética , Análise de Sequência de DNARESUMO
Candida wangnamkhiaoensis is a species clustered under the Hyphopichia clade has not ever been isolated from any clinical specimens. To the best of our knowledge, this is the first report of C. wangnamkhiaoensis associated with fungaemia in immunocompromised paediatric patient. The isolate was assigned a strain name as UZ1679/14, in which the identification was confirmed by a polymerase chain reaction-sequencing of the internal transcribed spacer (ITS) and large subunit (LSU) regions of the rRNA gene. Antifungal susceptibility pattern showed that the isolate was sensitive to anidulafungin, caspofungin, fluconazole and voriconazole. The patient clinically improved after the antifungal treatment with caspofungin.
Assuntos
Candida/isolamento & purificação , Candidemia/microbiologia , Hospedeiro Imunocomprometido , Anidulafungina , Antifúngicos/farmacologia , Candida/classificação , Candida/genética , Candida/ultraestrutura , Candidemia/tratamento farmacológico , Candidemia/imunologia , Caspofungina , Pré-Escolar , DNA Espaçador Ribossômico , Equinocandinas/farmacologia , Equinocandinas/uso terapêutico , Fluconazol/farmacologia , Humanos , Hifas/isolamento & purificação , Hifas/ultraestrutura , Lipopeptídeos/farmacologia , Lipopeptídeos/uso terapêutico , Masculino , Filogenia , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Voriconazol/farmacologiaRESUMO
The number of new fungal pathogens is increasing due to growing population of immunocompromised patients and advanced identification techniques. Fereydounia khargensis is a yeast and was first described in 2014 from environmental samples. As far as we know, this is the first report of human infections associated with F. khargensis. The yeasts were isolated from blood of a HIV-positive patient and pleural fluid of chronic renal failure patient. Amplification and sequencing of the internal transcribed spacer and the large subunit regions confirmed the identity of the isolates. Both isolates showed multi-drug resistance to antifungal agents tested.