Isolamentos bacterianos de fetos abortados bovinos examinados no Instituto Biológico de Säo Paulo, no período de 1985 a 1992 / Bacterial isolations from bovine aborted fetuses examined in Instituto Biologico of Sao Paulo State, Brazil, from 1985 to 1992

No período de 1985 a 1992, foram analisadas bacteriologicamente 544 amostras de órgäos e anexos fetais, provenientes de 282 fetos bovinos, oriundos de rebanhos, na sua maioria leiteiros, procedentes de vários estados do Brasil. Foram consideradas como possíveis causas de abortamento, as bactérias patogênicas e as culturas puras ou preponderantes de bactérias oportunistas. Excluindo-se os materiais impróprios para exame (25/282), dos 257 restantes, em 37,4 por cento foram diagnosticadas causas bacterianas, tais como: Brucella abortus (6,2 por cento), Leptospira spp (6,2 por cento), Staphylococcus aureus (5,4 por cento), Campylobacter fetus (4,7 por cento) e Streptococcus Beta hemolítico (3,5 por cento). Os focos destes agentes apresentavam-se amplamente distribuídos no Estado de Säo Paulo

Mannose-resistant haemagglutination and colonisation factors among Escherichia coli strains isolated from pigs.

Ten heat-labile (LT) and 23 heat-stable (ST) enterotoxin-producing strains of enterotoxigenic Escherichia coli (ETEC) and 100 non-enterotoxigenic E coli (non-ETEC) strains isolated from pigs with diarrhoea were examined for the presence of colonisation factors by means of mannose-resistant haemagglutination and serological tests. Seven of 10 LT strains, one of 23 ST and 18 of 100 non-ETEC carried K88 antigen; and four ST and four non-ETEC strains possessed CFA/I antigen. All strains carrying K88, K99 and CFA/I antigens were positive in the mannose-resistant microhaemagglutination (MRMH) test but four ST and 14 non-ETEC isolates positive in the MRMH test did not possess K88, K99 or CFA/I antigens. A considerable number of non-ETEC strains were positive in the MRMH test but lacked K88 and K99 antigens, suggesting that other unknown haemagglutinating colonisation factors may exist in porcine E coli strains.

Pesquisas de aglutininas anti-leptospira em suinos aparentemente sadios abatidos em matadouro. / Research on anti-leptospira aglutinins in swines apparently healthy from abattoirs

Os autores examinaram 500 amostras de soros de suinos aparentemente normais, abatidos em um matadouro proximo a cidade de Sao Paulo.A tecnica usada foi a soro-aglutinacao microscopica com leitura em campo escuro, tendo-se empregado como antigenos 12 sorotipos de leptospiras vivas. Encontraram-se 16,2% de soros positivos, com predominancia para os sorotipos pomona, wolffi e grippotyphosa. Os titulos variaram de 1/200 a 1/3.200, sendo que nesta ultimma diluicao houve apenas um soro positivo para o sorotipo pomona. A taxa de positividade e a possibilidade de os animais positivos estarem na condicao de reservatorio de leptospiras sao tambem abordadas

Infeccao estreptococica em cobaias (Cavia cobaya). / Streptococcal infection in guinea pigs (Cavia cobaya)

Dois surtos de adenite cervical em cobaias (Cavia cobaya) ocorreram de forma simultanea nos bioterios do Centro Panamericano de Febre Aftosa, Rio de Janeiro e na Secao de Imunologia do Instituto Biologico de Sao Paulo. Alem do comprometimento dos linfonodos cervicais, varias cobaias tambem apresentaram lesoes oculares com variavel grau de intensidade. De ambas epizootias, isolou-se o S. zooepidemicus, conforme constatado atraves de provas bioquimicas e sorologicas. A partir do agente isolado, foi possivel a reproducao da doenca. A histopatologia permitiu evidenciar processos inflamatorios, comprometendo diversas estruturas do globo ocular com tecido de granulacao, infiltracao linfo-histiocitaria e vasos de neoformacao. Medidas profilaticas e administracao diaria de quimioterapico, 5% (p/p) de sulfamilamida na racao, foram eficazes para o controle da enfermidade

Two new leptospiral serovars in the Hebdomadis group isolated from cattle in Brazil.

Two leptospiral serovars belonging to the Hebdomadis Serogroup are described. These strains are represented by two isolations from apparently healthy zebu cattle. It is proposed that the serovars be designated guaicurus strain Bov. G and goiano strain Bov. 131.

[Immuno-stimulating effect of levamisole in the immunization of guinea pigs vaccinated against brucellosis]. / Ação immuno-estimulante do levamisole na imunização de cobaios vacinados contra a brucelose.

The immuno-stimulating Levamisole action was checked by the authors in guinea-pigs vaccinated against brucellosis and exposed to the challenge test afterwards against 2.308 Brucella abortus pathogenic strain. Twelve lots of eight guinea-pigs were used disposed in the following distribution: Lots I, III, IV were vaccinated with B-19 strain vaccine; lots II, IV and VI were vaccinated with the same vaccine and, after 48 hours, received the Levamisole injection. Lots VII VIII received only the Levamisole injection and the pathogenic Brucella strain, forming the control groups. The vaccinated guinea-pigs showed a higher protection against challenge, always above 75% in relation to the lots that were only vaccinated, demonstrating a dear immuno-stimulating action of the essayed product. Considering the obtained results in this assay, the authors speculate on the anti-brucellosis vaccines potentialization with immuno-stimulating products, as Levamisole.

Serological study of listeriosis in domestic animals in São Paulo-Brazil.

A serological examination was carried out for L. monocytogenes antibodies on 9,318 domestic animal--7,809 bovine, 838 horses and 671 swine--in São Paulo, Brazil. Serum agglutination in tubes was the method used. Only somatic antigens from serotypes 1, 2, 3, 4a and 4b were employed. It was considered reagent all sera reacting in a titer 1/20 while as positive only sera with 1/320 or above. According this criteria the results showed showed that in cattle 17.8% were reagent but only 8 sera were positive for types 1, 2 and 4b. Only type 1 was found in horses in a rate of 22.7% reagent, and 11 positive samples. For swine the percentage of reagent was 13.3% but none serum was positive, since the highest titer was 1/160.

Leptospirosis in wildlife in Brazil: isolation of a new serotype in the pyrogenes group.

A new leptospiral serotype in the serogroup Pyrogenes is described. The strain was isolated from one gray "four-eyed" opossum (Philander opossum). It is proposed that the serotype be designated guaratuba, strain An-7705. The isolations of serotypes ballum from the South American field mouse (Akodon arviculoides), szwajizak and icterohaemorrhagiae from the North American opossum (Didelphis marsupialis), and grippotyphosa from many other wild animal species as well as domesticated animals are also described.