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1.
PLoS One ; 6(2): e15786, 2011 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-21347250

RESUMO

Dickkopf1 (Dkk1) is a Wnt/ß-catenin inhibitor that participates in many processes during embryonic development. One of its roles during embryogenesis is to induce head formation, since Dkk1-null mice lack head structures anterior to midbrain. The Wnt/ß-catenin pathway is also known to regulate different aspects of ventral midbrain (VM) dopaminergic (DA) neuron development and, in vitro, Dkk1-mediated inhibition of the Wnt/ß-catenin pathway improves the DA differentiation in mouse embryonic stem cells (mESC). However, the in vivo function of Dkk1 on the development of midbrain DA neurons remains to be elucidated. Here we examined Dkk1(+/-) embryos and found that Dkk1 is required for the differentiation of DA precursors/neuroblasts into DA neurons at E13.5. This deficit persisted until E17.5, when a defect in the number and distribution of VM DA neurons was detected. Furthermore, analysis of the few Dkk1(-/-) embryos that survived until E17.5 revealed a more severe loss of midbrain DA neurons and morphogenesis defects. Our results thus show that Dkk1 is required for midbrain DA differentiation and morphogenesis.


Assuntos
Diferenciação Celular , Neurônios Dopaminérgicos/citologia , Neurônios Dopaminérgicos/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Mesencéfalo/citologia , Mesencéfalo/crescimento & desenvolvimento , Morfogênese , Animais , Contagem de Células , Regulação da Expressão Gênica no Desenvolvimento , Peptídeos e Proteínas de Sinalização Intercelular/deficiência , Peptídeos e Proteínas de Sinalização Intercelular/genética , Mesencéfalo/metabolismo , Camundongos , Mutação , Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/metabolismo
2.
Cell Stem Cell ; 5(2): 178-90, 2009 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-19664992

RESUMO

Adult neurogenesis persists in the subventricular zone and the dentate gyrus and can be induced upon central nervous system injury. However, the final contribution of newborn neurons to neuronal networks is limited. Here we show that in neural stem cells, stimulation of the "death receptor" CD95 does not trigger apoptosis but unexpectedly leads to increased stem cell survival and neuronal specification. These effects are mediated via activation of the Src/PI3K/AKT/mTOR signaling pathway, ultimately leading to a global increase in protein translation. Induction of neurogenesis by CD95 was further confirmed in the ischemic CA1 region, in the naive dentate gyrus, and after forced expression of CD95L in the adult subventricular zone. Lack of hippocampal CD95 resulted in a reduction in neurogenesis and working memory deficits. Following global ischemia, CD95-mediated brain repair rescued behavioral impairment. Thus, we identify the CD95/CD95L system as an instructive signal for ongoing and injury-induced neurogenesis.


Assuntos
Células-Tronco Adultas/metabolismo , Isquemia Encefálica/metabolismo , Encéfalo/metabolismo , Proteína Ligante Fas/metabolismo , Neurogênese/fisiologia , Receptor fas/metabolismo , Células-Tronco Adultas/transplante , Animais , Isquemia Encefálica/terapia , Feminino , Expressão Gênica/fisiologia , Memória/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Neurônios/metabolismo , Proteínas Quinases/metabolismo , Transdução de Sinais/fisiologia , Transplante de Células-Tronco , Serina-Treonina Quinases TOR
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