Effects of the level and source of dietary physically effective fiber on feed intake, nutrient utilization, heat energy, ruminal fermentation, and milk production by Alpine goats.

Thirty-two primiparous and 31 multiparous Alpine goats were used to determine influences of diets varying in level and source of forage on performance in early to mid-lactation for 16 wk. Diets consisted of 40%, 50%, 60%, and 70% forage (designated as 40F, 50F, 60F, and 70F, respectively) with 60F and 70F containing coarsely ground grass hay (primarily orchardgrass) and 40F and 50F containing cottonseed hulls, alfalfa pellets, and coarsely ground wheat hay. Diets contained 15.9% to 16.3% crude protein and 37.8%, 42.1%, 53.5%, and 55.4% neutral detergent fiber (NDF) with 10.0%, 15.8%, 50.1%, and 55.5% particle retention on a 19-mm sieve, and 26.1%, 29.6%, 38.3%, and 40.0% physically effective NDF (peNDF) for 40F, 50F, 60F, and 70F, respectively. Dry matter intake (2.71, 2.75, 1.96, and 1.95 kg/d) and milk yield (2.82, 2.71, 2.23, and 2.10 kg/d for 40F, 50F, 60F, and 70F, respectively) were lower (P < 0.05) for the two diets highest in forage. Digestion of organic matter was similar among diets (P > 0.05), but digestibility of NDF was greater (P < 0.05) for 60F and 70F (57.5%, 58.4%, 68.9%, and 72.2% for 40F, 50F, 60F, and 70F, respectively). Diet affected (P < 0.05) milk fat (3.16%, 3.37%, 2.93%, and 2.97%) and protein concentrations (2.62%, 2.69%, 2.58%, and 2.52% for 40F, 50F, 60F, and 70F, respectively). Milk energy yield was greater (P < 0.05) for the two diets lowest in forage (7.51, 7.45, 5.68, and 5.34 MJ/d), although yield relative to dry matter intake was not affected (P > 0.05) by diet and was lower (P < 0.05) for primiparous vs. multiparous goats (2.71 and 3.09 MJ/kg). Ruminal pH and acetate proportion were greater for 60F and 70F than for the other diets and the proportion of butyrate was lower for the two diets highest in fiber. The mean lengths of time spent ruminating, eating, standing, and lying were not affected (P > 0.05) by diet or parity, but many interactions involving diet, period, hour, and parity were significant (P < 0.05). In conclusion, lactational performance of Alpine goats in early to mid-lactation will be constrained with diets high in forage of moderate quality, peNDF content, and large particle size, which appeared related to limited feed intake.

Patterns and determinants of the global herbivorous mycobiome.

Despite their role in host nutrition, the anaerobic gut fungal (AGF) component of the herbivorous gut microbiome remains poorly characterized. Here, to examine global patterns and determinants of AGF diversity, we generate and analyze an amplicon dataset from 661 fecal samples from 34 mammalian species, 9 families, and 6 continents. We identify 56 novel genera, greatly expanding AGF diversity beyond current estimates (31 genera and candidate genera). Community structure analysis indicates that host phylogenetic affiliation, not domestication status and biogeography, shapes the community rather than. Fungal-host associations are stronger and more specific in hindgut fermenters than in foregut fermenters. Transcriptomics-enabled phylogenomic and molecular clock analyses of 52 strains from 14 genera indicate that most genera with preferences for hindgut hosts evolved earlier (44-58 Mya) than those with preferences for foregut hosts (22-32 Mya). Our results greatly expand the documented scope of AGF diversity and provide an ecologically and evolutionary-grounded model to explain the observed patterns of AGF diversity in extant animal hosts.

Genetic Selection for Resistance to Gastrointestinal Parasitism in Meat Goats and Hair Sheep through a Performance Test with Artificial Infection of Haemonchus contortus.

Internal parasitism has been the leading cause of morbidity and mortality in small ruminants in many areas such as the southcentral USA. Among the different approaches and management practices to cope with internal parasitism, genetic selection for internal parasite resistance is recognized as one with considerable potential long-term impact. A central performance test with artificial infection of Haemonchus contortus for selection of growing meat goats and hair sheep for breeding to increase resistance to internal parasitism and on-farm selection of females was conducted for 3 years. The results varied considerably among breeds of goats and flocks of sheep. Spanish goats and St. Croix sheep maintained relatively low fecal egg count (FEC) each year, whereas for goats categorized as being of high resistance and Dorper sheep FEC decreased with advancing year. Packed call volume (PCV) and total serum immunoglobulin (Ig) levels were not strongly related to FEC. Genetic parameters varied between the two species, which might be related to previous selection pressure exerted for parasite resistance. Heritability of FEC was higher in goats than sheep. The genetic correlation between FEC and IgM and IgG was negative for both species, which suggests possible genetic association. Genetic and phenotypic correlations between ADG and FEC were nonsignificant for both species. In conclusion, different relationships of FEC and PCV between species require careful attention during selection and the lack of relationship between ADG and FEC suggests that selection of growing male meat goats and hair sheep for resistance to internal parasitism will not adversely affect growth performance.

Effects of restricted availability of drinking water on body weight and feed intake by Dorper, Katahdin, and St. Croix sheep from different regions of the USA.

Resilience to restricted availability of drinking water was evaluated with 44 Dorper (DOR; initial age = 3.7 ± 0.34 yr), 42 Katahdin (KAT; 3.9 ± 0.36 yr), and 42 St. Croix (STC; 2.7 ± 0.29 yr) sheep from 46 farms in 4 regions of the USA (Midwest, MW; Northwest, NW; Southeast, SE; central Texas, TX). Ad libitum water intake was determined during 2 wk of period 1, with 75% of this amount offered in 2 wk of period 2 and 50% in 5 wk (i.e., 5 to 9) of period 3. Body weight and DMI in week 2 of period 1 and week 9 of period 3 were analyzed with a mixed effects model. There was a breed × period interaction (P = 0.023) in water intake relative to BW (6.17, 6.69, and 7.19 in period 1 and 3.04%, 3.26%, and 3.36% BW in period 3 for DOR, KAT, and STC, respectively; SEM = 0.219). There were 3-way interactions of breed, region, and period in BW and DMI in g/d (P < 0.010). For STC, BW was greater (P < 0.05) in period 3 vs. 1 for all regions (50.1, 47.6, 42.4, and 45.8 kg in period 1 and 51.9, 49.3, 44.5, and 47.7 kg in period 3), whereas there was only a period difference for DOR from the MW and SE (61.4, 66.0, 64.6, and 59.6 kg in period 1 and 60.6, 66.5, 65.7, and 62.4 kg) and for KAT from TX (50.1, 47.6, 42.4, and 45.8 kg in period 1 and 51.9, 49.3, 44.5, and 47.7 kg in period 3 for MW, NW, SE, and TX, respectively; SEM = 2.57). In accordance, DMI by STC was similar (P > 0.05) between periods for all regions (1.25, 1.17, 1.06, and 1.16 kg/d in period 1 and 1.12, 1.08, 1.02, and 1.02 kg/d in period 3), as was also the case for DOR from MW and SE but not from NW or TX (1.54, 1.50, 1.30, and 1.41 kg/d in period 1 and 1.41, 1.13, 1.25, and 1.18 kg/d in period 3) and KAT from TX though not from the other 3 regions (1.47, 1.52, 1.48, and 1.40 kg/d in period 1 and 1.06, 1.15, 1.30, and 1.33 kg/d in period 3 for MW, NW, SE, and TX, respectively; SEM = 0.061). In conclusion, based on BW and DMI with water intake restricted at 50% of ad libitum consumption by individual animals, STC appeared more consistent in display of high resilience to restricted water availability, although DOR from 2 regions and KAT from 1 also were relatively resilient. The results suggest benefit to breed comparisons of inclusion of animals from multiple areas and that environmental conditions of regions may have disparate effects with different breeds of hair sheep.

Signatures of selection for resistance to Haemonchus contortus in sheep and goats.

BACKGROUND: Gastrointestinal nematode infection (GNI) is the most important disease affecting the small ruminant industry in U.S. The environmental conditions in the southern United States are ideal for the survival of the most pathogenic gastrointestinal nematode, Haemonchus contortus. Host genetic variation for resistance to H. contortus allows selective breeding for increased resistance of animals. This selection process increases the prevalence of particular alleles in sheep and goats and creates unique genetic patterns in the genome of these species. The aim of this study was to identify loci with divergent allelic frequencies in a candidate gene panel of 100 genes using two different approaches (frequentist and Bayesian) to estimate Fst outliers in three different breeds of sheep and goats exposed to H. contortus. RESULTS: Our results for sheep populations showed SNPs under selection in C3AR1, CSF3, SOCS2, NOS2, STAT5B, TGFB2 and IL2RA genes using frequentist and Bayesian approaches. For goats, SNPs in CD1D, ITGA9, IL12A, IL13RA1, CD86 and TGFB2 genes were under selection. Common signatures of selection in both species were observed in NOS2, TGFB2 and TLR4 genes. Directional selection was present in all SNPs evaluated in the present study. CONCLUSIONS: A total of 13 SNPs within 7 genes of our candidate gene panel related to H. contortus exposure were identified under selection in sheep populations. For goats, 11 SNPs within 7 genes were identified under selection. Results from this study support the hypothesis that resistance to H. contortus is likely to be controlled by many loci. Shared signatures of selection related to mechanisms of immune protection against H. contortus infection in sheep and goats could be useful targets in breeding programs aimed to produce resistant animals with low FEC.

Effects of lespedeza condensed tannins alone or with monensin, soybean oil, and coconut oil on feed intake, growth, digestion, ruminal methane emission, and heat energy by yearling Alpine doelings.

Fifty-four Alpine doelings (initial BW and age of 31.7 ± 0.38 kg and 306 ± 1.9 d, respectively) were allocated to nine treatments individually fed for ad libitum intake of 25% concentrate and 75% forage diets (DM basis). Alfalfa was the forage in the control diet. Other diets contained Sericea lespedeza as the forage, with 1.25% DM of quebracho extract included in the concentrate fraction for a dietary condensed tannin level of 8.4%. Lespedeza treatments were no additive (L) and inclusion of monensin (I) at 22 mg/kg DM (L-I), soybean oil at 3% (L-S), coconut oil at 3% (L-N), I and 3% soybean oil (L-I-S), I and 3% coconut oil (L-I-N), 1.5% soybean oil and 1.5% coconut oil (L-S-N), and I, 1.5% soybean oil, and 1.5% coconut oil (L-I-S-N). The experiment was 12 wk with two 6-wk periods. Gas exchange was determined in weeks 6 and 12, and other measures occurred in weeks 5 and 11. The control diet offered averaged 2.67% nitrogen, 43.8% neutral detergent fiber, and 8.8% acid detergent lignin, and the L diet offered averaged 2.03% nitrogen, 42.8% neutral detergent fiber, and 13.2% acid detergent lignin. There were no treatment × period interactions for digestibilities (P ≥ 0.770) or methane emission (P ≥ 0.324). There were differences (P < 0.001) between the control treatment and diets with lespedeza in intake of DM (1.46, 1.23, 1.30, 1.18, 1.32, 1.10, 1.02, 1.20, and 1.01 kg/d; SEM = 0.059), digestibility of OM (57.4%, 50.9%, 51.8%, 52.7%, 50.3%, 52.1%, 52.1%, 51.9%, and 49.8%; SEM = 1.42), and digestibility of nitrogen (59.1%, 31.2%, 32.5%, 37.1%, 31.6%, 38.3%, 30.4%, 38.4%, and 34.1% for control, L, L-I, L-S, L-N, L-I-S, L-I-N, L-S-N, and L-I-S-N, respectively; SEM = 2.21). Ruminal methane emission was less (P < 0.001) for diets with lespedeza than for the control in MJ/d (1.36, 0.76, 0.84, 0.71, 0.71, 0.66, 0.65, 0.68, and 0.68; SEM = 0.048) and relative to intake of gross energy (5.92%, 3.27%, 3.49%, 3.19%, 2.84%, 2.91%, 3.20%, 3.20%, and 3.27%; SEM = 0.165) and digestible energy (11.19%, 6.98%, 7.40%, 6.38%, 5.90%, 5.69%, 6.37%, 6.38%, and 6.70% for control, L, L-I, L-S, L-N, L-I-S, L-I-N, L-S-N, and L-I-S-N, respectively; SEM = 0.400). In conclusion, the magnitude of effect of condensed tannins from lespedeza and quebracho extract on ruminal methane emission by Alpine doelings did not diminish over time and was not markedly influenced by dietary inclusion of monensin, soybean oil, or coconut oil.