DESI-MS as a tool for direct lipid analysis in cultured cells.

Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface preparation of cell culture slides, induction of oxidative stress, and cell monolayer culture preparation as well as optimization of the analysis. Advantages and drawbacks of the method were also discussed.

Potential link between MHC-self-peptide presentation and hematopoiesis; the analysis of HLA-DR expression in CD34-positive cells and self-peptide presentation repertoires of MHC molecules associated with paroxysmal nocturnal hemoglobinuria.

The mechanisms of MHC allele associations with paroxysmal nocturnal hemoglobinuria (PNH) and its aplastic anemia subtype (AA/PNH) remain unclear. It might be dependent on MHC molecule functional properties, such as a scope and frequency of antigen sampling and presentation. For documented PNH-associated MHC alleles we analyzed current reference databases on MHC molecule-eluted peptide presentation repertoires and searched for a range of presented peptides. MHC class II expression was measured on CD34+ cells and appeared to be increased in PNH patients. Two class I alleles (HLA-A*24:02 and B*18:01) have been previously confirmed to associate with protection and increased risk of AA/PNH, respectively. Their product molecules presented immunodominant epitopes derived from proapoptotic (serine/threonine-protein phosphatase) and antiapoptotic (phospholipase D), respectively, intracellular enzymes dependent on phosphoinositide (PI) content. For total PNH and non-aplastic PNH (n/PNH) subtype-associated DRB1*15:01 and DRB1*04:01 class II molecules presentation of exceptionally broad arrays of their own peptide fragments has been found. We conclude that self antigen peptides presented with high frequency in the context of MHC molecules of increased expression may be involved in the immune recognition and the regulation of HSC in the periphery. The block in the normal plasma membrane PI production due to the PIG-A mutation can help explain the differences in the activation of intracellular regulatory pathways observed between PNH and normal HSC. This is evident in the variation in MHC association patterns and peptide presentation repertoires between these two groups of patients.

The relationship between the need for closure and support for military action against Iraq: moderating effects of national attachment.

A variety of studies suggest that a high need for closure--that is, a desire for knowledge that is clear, stable, and unambiguous as opposed to confusing or uncertain--may be associated with greater hostility toward relevant outgroups. Using international attitudes as the context, the authors examine the hypothesis that the relationship between the need for closure and support for military action against Iraq may be moderated by identification with the national ingroup. Specifically, it is expected that this relationship will be moderated by nationalism (i.e., an aggressive form of identification based on a desire for national dominance) but not patriotism (i.e., a more neutral love of one's country). The data provided a clear pattern of support for this hypothesis and additional analyses indicated that a high need for closure reduced variability about the use of force among the highly nationalistic but not the highly patriotic.

Understanding responses to political conflict: interactive effects of the need for closure and salient conflict schemas.

Two studies examined the relationship between the need for cognitive closure and preferences for conflict-resolution strategies in 2 different samples of elite political actors. Although research has suggested that high need for closure should be associated with competitiveness, the authors argue that this relationship should be strongest among political actors with a hostile conflict schema, or representation of what a conflict is and how it should be dealt with. The authors provide evidence for this hypothesis using archival survey data on American foreign-policy officials' attitudes toward international conflict at the height of the Cold War (Study 1) and their own data on the relationship between the need for closure and conflict-strategy preferences among samples of activists from 2 political parties in Poland: a centrist party with a reputation for cooperativeness and an extremist party with a reputation for confrontation (Study 2). The broader implications of these findings are discussed.