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1.
Nat Ecol Evol ; 1(10): 1585, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-29185503

RESUMO

In Fig. 5 of the version of this Article originally published, the final number on the x axes of each panel was incorrectly written as 1.5; it should have read 7.5. This has now been corrected in all versions of the Article.

2.
Nat Ecol Evol ; 1(5): 119, 2017 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-28812690

RESUMO

Fixed chromosomal inversions can reduce gene flow and promote speciation in two ways: by suppressing recombination and by carrying locally favoured alleles at multiple loci. However, it is unknown whether favoured mutations slowly accumulate on older inversions or if young inversions spread because they capture pre-existing adaptive quantitative trait loci (QTLs). By genetic mapping, chromosome painting and genome sequencing, we have identified a major inversion controlling ecologically important traits in Boechera stricta. The inversion arose since the last glaciation and subsequently reached local high frequency in a hybrid speciation zone. Furthermore, the inversion shows signs of positive directional selection. To test whether the inversion could have captured existing, linked QTLs, we crossed standard, collinear haplotypes from the hybrid zone and found multiple linked phenology QTLs within the inversion region. These findings provide the first direct evidence that linked, locally adapted QTLs may be captured by young inversions during incipient speciation.

3.
Nat Genet ; 46(9): 982-8, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25064006

RESUMO

The cultivation of rice in Africa dates back more than 3,000 years. Interestingly, African rice is not of the same origin as Asian rice (Oryza sativa L.) but rather is an entirely different species (i.e., Oryza glaberrima Steud.). Here we present a high-quality assembly and annotation of the O. glaberrima genome and detailed analyses of its evolutionary history of domestication and selection. Population genomics analyses of 20 O. glaberrima and 94 Oryza barthii accessions support the hypothesis that O. glaberrima was domesticated in a single region along the Niger river as opposed to noncentric domestication events across Africa. We detected evidence for artificial selection at a genome-wide scale, as well as with a set of O. glaberrima genes orthologous to O. sativa genes that are known to be associated with domestication, thus indicating convergent yet independent selection of a common set of genes during two geographically and culturally distinct domestication processes.


Assuntos
Genoma de Planta , Oryza/genética , África , Sequência de Aminoácidos , Sequência de Bases , Produtos Agrícolas/genética , DNA de Plantas/genética , Variação Genética , Genética Populacional/métodos , Dados de Sequência Molecular , Análise de Sequência de DNA/métodos
4.
Genetics ; 187(4): 1023-30, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21321134

RESUMO

The genus Drosophila has been the subject of intense comparative phylogenomics characterization to provide insights into genome evolution under diverse biological and ecological contexts and to functionally annotate the Drosophila melanogaster genome, a model system for animal and insect genetics. Recent sequencing of 11 additional Drosophila species from various divergence points of the genus is a first step in this direction. However, to fully reap the benefits of this resource, the Drosophila community is faced with two critical needs: i.e., the expansion of genomic resources from a much broader range of phylogenetic diversity and the development of additional resources to aid in finishing the existing draft genomes. To address these needs, we report the first synthesis of a comprehensive set of bacterial artificial chromosome (BAC) resources for 19 Drosophila species from all three subgenera. Ten libraries were derived from the exact source used to generate 10 of the 12 draft genomes, while the rest were generated from a strategically selected set of species on the basis of salient ecological and life history features and their phylogenetic positions. The majority of the new species have at least one sequenced reference genome for immediate comparative benefit. This 19-BAC library set was rigorously characterized and shown to have large insert sizes (125-168 kb), low nonrecombinant clone content (0.3-5.3%), and deep coverage (9.1-42.9×). Further, we demonstrated the utility of this BAC resource for generating physical maps of targeted loci, refining draft sequence assemblies and identifying potential genomic rearrangements across the phylogeny.


Assuntos
Cromossomos Artificiais Bacterianos/genética , Cromossomos de Insetos/genética , Drosophila melanogaster/genética , Genoma de Inseto , Biblioteca Genômica , Animais , Evolução Biológica , Mapeamento Cromossômico , Genes de Insetos , Filogenia , Análise de Sequência de DNA
5.
Nat Genet ; 43(2): 101-8, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21186351

RESUMO

We sequenced and assembled the draft genome of Theobroma cacao, an economically important tropical-fruit tree crop that is the source of chocolate. This assembly corresponds to 76% of the estimated genome size and contains almost all previously described genes, with 82% of these genes anchored on the 10 T. cacao chromosomes. Analysis of this sequence information highlighted specific expansion of some gene families during evolution, for example, flavonoid-related genes. It also provides a major source of candidate genes for T. cacao improvement. Based on the inferred paleohistory of the T. cacao genome, we propose an evolutionary scenario whereby the ten T. cacao chromosomes were shaped from an ancestor through eleven chromosome fusions.


Assuntos
Cacau/genética , Genoma de Planta , Núcleo Celular/genética , DNA/genética , Elementos de DNA Transponíveis , Evolução Molecular , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Genótipo , Homozigoto , Hibridização In Situ , Modelos Genéticos , Locos de Características Quantitativas
6.
BMC Genomics ; 11: 395, 2010 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-20569427

RESUMO

BACKGROUND: Genetically anchored physical maps of large eukaryotic genomes have proven useful both for their intrinsic merit and as an adjunct to genome sequencing. Cultivated tetraploid cottons, Gossypium hirsutum and G. barbadense, share a common ancestor formed by a merger of the A and D genomes about 1-2 million years ago. Toward the long-term goal of characterizing the spectrum of diversity among cotton genomes, the worldwide cotton community has prioritized the D genome progenitor Gossypium raimondii for complete sequencing. RESULTS: A whole genome physical map of G. raimondii, the putative D genome ancestral species of tetraploid cottons was assembled, integrating genetically-anchored overgo hybridization probes, agarose based fingerprints and 'high information content fingerprinting' (HICF). A total of 13,662 BAC-end sequences and 2,828 DNA probes were used in genetically anchoring 1585 contigs to a cotton consensus genetic map, and 370 and 438 contigs, respectively to Arabidopsis thaliana (AT) and Vitis vinifera (VV) whole genome sequences. CONCLUSION: Several lines of evidence suggest that the G. raimondii genome is comprised of two qualitatively different components. Much of the gene rich component is aligned to the Arabidopsis and Vitis vinifera genomes and shows promise for utilizing translational genomic approaches in understanding this important genome and its resident genes. The integrated genetic-physical map is of value both in assembling and validating a planned reference sequence.


Assuntos
Genoma de Planta/genética , Gossypium/genética , Mapeamento Físico do Cromossomo/métodos , Arabidopsis/genética , Cloroplastos/genética , Cromossomos Artificiais Bacterianos/genética , Sequência Consenso , Mapeamento de Sequências Contíguas , Impressões Digitais de DNA , Evolução Molecular , Duplicação Gênica , Genes de Plantas/genética , Loci Gênicos/genética , Marcadores Genéticos/genética , Gossypium/citologia , Hibridização de Ácido Nucleico , Biossíntese de Proteínas , Sequências Repetitivas de Ácido Nucleico , Vitis/genética
7.
PLoS Genet ; 5(11): e1000728, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19936048

RESUMO

Most of our understanding of plant genome structure and evolution has come from the careful annotation of small (e.g., 100 kb) sequenced genomic regions or from automated annotation of complete genome sequences. Here, we sequenced and carefully annotated a contiguous 22 Mb region of maize chromosome 4 using an improved pseudomolecule for annotation. The sequence segment was comprehensively ordered, oriented, and confirmed using the maize optical map. Nearly 84% of the sequence is composed of transposable elements (TEs) that are mostly nested within each other, of which most families are low-copy. We identified 544 gene models using multiple levels of evidence, as well as five miRNA genes. Gene fragments, many captured by TEs, are prevalent within this region. Elimination of gene redundancy from a tetraploid maize ancestor that originated a few million years ago is responsible in this region for most disruptions of synteny with sorghum and rice. Consistent with other sub-genomic analyses in maize, small RNA mapping showed that many small RNAs match TEs and that most TEs match small RNAs. These results, performed on approximately 1% of the maize genome, demonstrate the feasibility of refining the B73 RefGen_v1 genome assembly by incorporating optical map, high-resolution genetic map, and comparative genomic data sets. Such improvements, along with those of gene and repeat annotation, will serve to promote future functional genomic and phylogenomic research in maize and other grasses.


Assuntos
Pareamento de Bases/genética , Genoma de Planta/genética , Zea mays/genética , Sequência de Bases , Cromossomos de Plantas/genética , Elementos de DNA Transponíveis/genética , Evolução Molecular , Duplicação Gênica , Rearranjo Gênico/genética , Genes de Plantas , Loci Gênicos/genética , Dados de Sequência Molecular , Mutação/genética , Fases de Leitura Aberta/genética , Oryza/genética , Mapeamento Físico do Cromossomo , RNA de Plantas/genética , Homologia de Sequência do Ácido Nucleico , Sorghum/genética , Sintenia/genética
8.
PLoS Genet ; 5(11): e1000715, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19936061

RESUMO

Maize is a major cereal crop and an important model system for basic biological research. Knowledge gained from maize research can also be used to genetically improve its grass relatives such as sorghum, wheat, and rice. The primary objective of the Maize Genome Sequencing Consortium (MGSC) was to generate a reference genome sequence that was integrated with both the physical and genetic maps. Using a previously published integrated genetic and physical map, combined with in-coming maize genomic sequence, new sequence-based genetic markers, and an optical map, we dynamically picked a minimum tiling path (MTP) of 16,910 bacterial artificial chromosome (BAC) and fosmid clones that were used by the MGSC to sequence the maize genome. The final MTP resulted in a significantly improved physical map that reduced the number of contigs from 721 to 435, incorporated a total of 8,315 mapped markers, and ordered and oriented the majority of FPC contigs. The new integrated physical and genetic map covered 2,120 Mb (93%) of the 2,300-Mb genome, of which 405 contigs were anchored to the genetic map, totaling 2,103.4 Mb (99.2% of the 2,120 Mb physical map). More importantly, 336 contigs, comprising 94.0% of the physical map ( approximately 1,993 Mb), were ordered and oriented. Finally we used all available physical, sequence, genetic, and optical data to generate a golden path (AGP) of chromosome-based pseudomolecules, herein referred to as the B73 Reference Genome Sequence version 1 (B73 RefGen_v1).


Assuntos
Genoma de Planta/genética , Zea mays/genética , Algoritmos , Sequência de Bases , Cromossomos Artificiais Bacterianos/genética , Cromossomos de Plantas/genética , Clonagem Molecular , Mapeamento de Sequências Contíguas , Marcadores Genéticos , Dados de Sequência Molecular , Fenômenos Ópticos , Mapeamento Físico do Cromossomo , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico
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