Suicidal ideation in adolescents with adiponectin receptor 2 rs12342 polymorphism affected by Wenchuan earthquake.

PURPOSE: The present study was to investigate prevalence of suicidal ideation and its associations with biological and environmental factors in adolescents with different genotypes of rs12342 at adiponectin receptor 2 gene (ADIPOR2). METHODS: Suicidal ideation, biological and environmental factors were evaluated by questionnaires in 669 high school students after Wenchuan earthquake in China. ADIPOR2 rs12342 was genotyped by polymerase chain reaction-restriction fragment length polymorphism and verified by DNA sequencing. RESULTS: Female adolescents had higher prevalence of suicidal ideation than male students in AG heterozygote and GG homozygote, but not AA homozygote. Prevalence of suicidal ideation was different in male, but not female, subjects with different genotypes. Genotype and allele frequencies were significantly different between male students with and without suicidal ideation, but not the female counterparts. Family history of mental disorders, extent of damage to property, carbohydrate intake and protein intake were associated with suicidal ideation in female subjects, while ADIPOR2 rs12342, father's educational level and previous trauma experience were associated with suicidal ideation in male subjects. CONCLUSION: ADIPOR2 rs12342 is associated with and has potential to interact with environmental factors on suicidal ideation in a gender-dependent manner in youth. These findings pave a novel way and perspective for precision inferences of suicidal ideation in subjects with different genetic backgrounds. ADIPOR2 rs12342 needs to be considered when intervening suicidal ideation, especially in adolescents.

Identification of genes associated with methotrexate resistance in methotrexate-resistant osteosarcoma cell lines.

BACKGROUND: This study aimed to better understand the mechanisms underlying methotrexate (MTX)-resistance in osteosarcoma. METHODS: The raw transcription microarray data GSE16089 collected from three MTX-sensitive osteosarcoma (Saos-2) cell samples and three MTX-resistant osteosarcoma (Saos-2) cell samples were downloaded from Gene Expression Omnibus. After data processing, the differentially expressed genes (DEGs) were identified. Next, DEGs were submitted to DAVID for functional annotation based on the GO (Gene Ontology) database, as well as pathway enrichment analysis based on the KEGG (Kyoto Encyclopedia of Genes and Genomes) database. Transcription factors (TFs) and tumor-associated genes (TAGs) were identified with reference to TRANSFAC and TAG, and TSGene databases, respectively. The protein-protein interaction (PPI) network of the gene-encoded products was constructed, and the subnetwork with the highest score was also detected using Search Tool for the Retrieval of Interacting Genes and BioNet package. RESULTS: A total of 690 up-regulated genes and down-regulated 626 genes were identified. Up-regulated DEGs (including AARS and PARS2) were associated to transfer RNA (tRNA) aminoacylation while down-regulated DEGs (including AURKA, CCNB1, CCNE2, CDK1, and CENPA) were correlated with mitotic cell cycle. Totally, 13 TFs (including HMGB2), 13 oncogenes (including CCNA2 and AURKA), and 19 tumor suppressor genes (TSGs) (including CDKN2C) were identified from the down-regulated DEGs. Ten DEGs, including nine down-regulated genes (such as AURKA, CDK1, CCNE2, and CENPA) and one up-regulated gene (GADD45A), were involved in the highest score subnetwork. CONCLUSION: AARS, AURKA, AURKB, CENPA, CCNB1, CCNE2, and CDK may contribute to MTX resistance via aminoacyl-tRNA biosynthesis pathway, cell cycle pathway, or p53 signaling pathway.

[Effects of APOC3 polymorphisms on the plasma lipids in healthy adolescents with different body mass index].

OBJECTIVE: To investigate the possible effects of apolipoprotein C I gene (APOC3) polymorphisms on plasma lipids in healthy adolescents with different body mass index (BMI). METHODS: Seven hundred and twenty three adolescents were divided into four groups according to BMI: group 1 CBMI= (17.80 +/- 0.75) kg/m2,n=180], group 2 [BMI = (19.39 +/- 0.32) kg/m2, n=182), group 3 [BMI= (20.68 +/- 0.43) kg/m2, n=1813 and group 4 [BMI= (23.40 +/- 2.05) kg/m2 ,n=180J. Fasting venous blood samples were collected, plasma lipids were determined and genome DNA was extracted for determining the genotypes of the APOC3 Sst I and -482C>T polymorphisms by PCR-RFLP. RESULTS: With the elevation of BMI, height and plasma high-density lipoprotein cholesterol decreased significantly (P<0.001 for both), body mass, waist circumference, hip circumference, waist/hip ratio, plasma triglycerides (TG), total cholesterol and low-density lipoprotein cholesterol levels increased significantly (P<0.001 for all). No significant differences in TG levels among Sst I genotypes were observed in group 1, group 2 and group 3; but in group 4, significant differences in TG levels among Sst I genotypes were observed, S2 carriers had higher TG levels than the adolescents with S1S1 genotype. No significant differences in plasma lipids among -482C>T genotypes were observed in all groups. CONCLUSION: The elevation of plasma TG levels by the S2 allele of APOC3 Sst I polymorphism is associated with BMI. It is possible that the reduction of body mass could favorably modulate the elevation of TG levels by S2 allele in healthy adolescents.

[Effects of apolipoprotein A1 gene rs670 and rs5069 polymorphisms on the plasma lipid profiles in healthy adolescents with different body mass index].

OBJECTIVE: To investigate the possible effects of apolipoprotein A1 gene (APOA1) rs670 and rs5069 polymorphisms on plasma lipid profiles in healthy adolescents with different body mass index (BMI). METHODS: Totally 723 adolescents were divided into four groups according to their BMI: group 1[BMI =(17.80 ± 0.75)kg/m2], group 2[BMI = (19.39 ± 0.32) kg/m²], group 3[BMI = (20.68 ± 0.43) kg/m²], and group 4[BMI=(23.40 ± 2.05) kg/m²]. Height, weight, waist circumference, hip circumference, blood pressure, heart rate, plasma lipids, and blood glucose were determined, BMI and waist to hip ratio (W/H ratio) were calculated,and genome DNA was extracted for analyzing the genotypes of the APOA1 rs670 and rs5069 polymorphisms by polymerase chain reaction-restriction fragment length polymorphism. RESULTS: No significant differences in height, weight, BMI, waist circumference, hip circumference, W/H ratio, blood pressure, heart rate, plasma lipids, and blood glucose between APOA1 rs670 or rs5069 genotypes were observed among group 1, group 2, and group 3. In group 4, A carriers of the rs670 polymorphism had significantly higher systolic blood pressure (P=0.017) and blood glucose levels (P=0.009) than the adolescents with the GG genotype. T carriers of the rs5069 polymorphism had significantly higher height (P=0.013), weight (P=0.011), and hip circumference (P=0.026) than the adolescents with the CC genotype. CONCLUSIONS: In healthy adolescents with higher BMI, APOA1 rs670 polymorphism is associated with systolic blood pressure and blood glucose levels. The elevation of systolic blood pressure and blood glucose levels in A carriers of APOA1 rs670 polymorphism may be favorably modulated by weight loss.

Efficacy and safety of pitavastatin versus simvastatin: a meta-analysis of randomized controlled trials.

BACKGROUND AND OBJECTIVES: Pitavastatin is the latest statin to be approved and has shown beneficial effects on plasma lipid profiles. The aim of the present meta-analysis was to assess both the efficacy and safety of pitavastatin versus simvastatin, one of the most commonly used statins. METHODS: A search of the MEDLINE, EMBASE, OVID and Cochrane Central Register of Controlled Trials (CENTRAL) databases was undertaken. Clinical trials evaluating the efficacy and safety of simvastatin versus pitavastatin, published up to February 2014, were identified. Trials were included if they (1) were randomized controlled trials (RCTs) of at least 12 weeks' duration; (2) included patients with primary hypercholesterolaemia or mixed dyslipidaemia; (3) studied outcomes included low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C); and (4) were published in the English language. A fixed-effects model was used for data analysis if no significant heterogeneity was present; otherwise a random-effects model was used. Efficacy is reflected by the mean difference in the percentage change of plasma lipid profiles. Treatment-emergent adverse events (TEAEs) are presented as risk ratio (RR). RESULTS: A total of 1,468 patients were included in the meta-analysis. The results indicated similar efficacy of pitavastatin (versus simvastatin) in lowering LDL-C. Pitavastatin also had similar effects to simvastatin on other major aspects of plasma lipids, including TC, TG and HDL-C. Somewhat in contrast to common belief (based on distinct metabolism by P450 subtypes), the two statins did not differ in the incidence of TEAE. CONCLUSIONS: In clinical trials, pitavastatin was comparable to simvastatin in both efficacy and safety profile. Large-scale, high-quality observational studies are required to determine whether the advantage of pitavastatin in metabolism profiles could be translated into noticeable benefits.

Effects of the C161T polymorphism in the gene of peroxisome proliferators activated receptor γ on changes of plasma lipid and apolipoprotein ratios induced by a high carbohydrate diet in a healthy Chinese Han young population.

BACKGROUND: Changes in the ratios of plasma lipids and apolipoproteins may be associated with diets and the C161T polymorphism in the gene of peroxisome proliferators activated receptor gamma (PPARgamma). As a result, this study was to investigate the effects of this polymorphism on changes of the ratios induced by a high-carbohydrate (high-CHO) diet. METHODS: After a washout diet of 54% carbohydrate for 7 days, 56 healthy young adults (22.89 +/- 1.80 years old) were given the high-CHO diet of 70% carbohydrate for 6 days. Height, weight, waist circumference (WC), glucose, triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), apolipoprotein (apo) AI, and apoB100 at baseline and before and after the high-CHO diet were measured. Body mass index (BMI), TG/HDL-C, log (TG/HDL-C), TC/HDL-C, LDL-C/HDL-C, and apoB100/apoAI were calculated. PPARgamma C161T was detected by a PCR-RFLP method. The relationship between the polymorphism and the ratios were analyzed. RESULTS: The female T carriers had higher BMI and WC than the female CC homozygotes at baseline and before and after the diet, higher glucose, TG/HDL-C and log (TG/HDL-C) before the diet. In males, when compared to the T carriers, the CC homozygotes had higher TG/HDL-C, log (TG/HDL-C) and apoB100/apoAI at baseline and before and after the diet, higher glucose at baseline, higher LDL-C/HDL-C and TC/HDL-C before and after the diet. Compared with those before the high-CHO diet, TC/HDL-C and LDL-C/HDL-C decreased after the diet regardless of gender and the genotypes. Decreased BMI and WC were observed in the male CC homozygotes but only decreased BMI in the female T carriers. Notably, decreased apoB100/apoAI was observed in the male T carriers, while elevated TG/HDL-C and log (TG/HDL-C) in the female CC homozygotes, and reduced glucose in the female T carriers. CONCLUSIONS: The results suggest that the interplay of gender, the PPARgamma C161T polymorphism and the high-CHO diet can change not only the ratios of plasma lipids and apolipoproteins but also the association of the ratios with plasma glucose, WC, and BMI in healthy Chinese young adults. Once confirmed by larger sample size and multi-center trials, the findings will provide a new scientific basis for personalized dietary intervention for the subjects with different PPARgamma C161T genotypes to reduce risks of CAD, especially in a country with a quarter of the world's population.

[The role of CD14 promoter - 159 C-> T polymorphism on changes of serum lipid ratios induced by high-carbohydrate/low-fat diets in healthy Chinese Han youth].

OBJECTIVE: To investigate the role of CD14 promoter - 159 C-> T polymorphism on ratios of serum lipids and its interaction on the ratios with a high-carbohydrate/low-fat (HC/LF) diet in a young and healthy Chinese Han population. METHODS: After a washout diet for seven days, fifty six healthy young subjects (22.89 +/- 1.80 years) were given the HC/LF diet for six days. Twelve-hour fasting venous blood samples were collected in the mornings of the first, the eighth and the fourteenth days. The serum lipid profiles and the CD14 -159 C->T polymorphism were analyzed. The ratios of triglyceride/high density lipoprotein-cholesterol (TG/HDL-c), log (TG/HDL-c), total cholesterol/high density lipoprotein-cholesterol (TC/HDL-c) and low density lipoprotein-cholesterol/high density lipoprotein-cholesterol (LDL-c/HDL-c) were calculated. RESULTS: The male carriers of the C allele had significantly higher TG/HDL-c and log (TG/HDL-c) than the female carriers at baseline, after the washout diet and after the HC/LF diet, higher TC/HDL-c at baseline and after the washout diet, and higher LDL-c/HDL-c only after the washout diet. The female subjects with the TT genotype had higher TG/HDL-c and log (TG/HDL-c) than the female carriers of the C allele at baseline, after the washout diet and after the HC/LF diet, higher LDL-c/HDL-c at baseline and after the HC/LF diet, and higher TC/HDL-c only after the washout diet. Compared with that before the HC/LF diet, TC/HDL-c was significantly decreased after the HC/LF diet regardless of gender and the genotype of the CD14 -159 polymorphism. LDL-c/HDL-c was significantly decreased in both the male and female carriers of the C allele. TG/HDL-c and log (TG/HDL-c) were significantly increased only in the female carriers of the C allele. CONCLUSION: In the subjects with C allele, the HC/LF diet is a minor factor and its effects on the lipid ratios can be masked by the effects of the C allele at CD14 -159. The interaction between the HC/LF diet and the C allele at CD14 -159 can decrease LDL-c/HDL-c in both males and females and increase TG/ HDL-c and log (TG/HDL-c) in the females.

[Effects of a high-carbohydrate diet on the serum lipid and apolipoprotein ratios in healthy young adults with different genotypes of APOA1 -75 G/A polymorphism].

OBJECTIVE: To investigate the effects of a high-carbohydrate diet on the lipid and apolipoprotein ratios in healthy young adults with different genotypes of the polymorphism at -75 site in the promoter region of the gene of apolipoprotein AI (APOA1). METHODS: Fifty-six subjects aged (22.89 +/- 1.80) years were given a wash-out diet for 7 days, followed by a high-carbohydrate diet for 6 days. The wash-out diet contained 15% protein, 31% fat, and 54% carbohydrate. The high-carbohydrate diet contained 15% protein, 15% fat, and 70% carbohydrate. Twelve-hour fasting serum lipids and apolipoproteins B100 and AI were measured on the mornings of the 1st, the 8th, and the 14th days from the beginning of the wash-out diet. The ratios of triglyceride (TG)/high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC)/HDL-C, low-density lipoprotein cholesterol (LDL-C)/HDL-C, and apolipoprotein B100 (APOB100)/apolipoprotein AI (APOAI) were calculated. The genome DNA was extracted and the polymorphism of APOA1 -75 G/A was determined by polymerase chain reaction followed by restriction fragment length polymorphism assay. RESULTS: At baseline, the lipid and apolipoprotein ratios showed no significant differences between the GG genotype and the A carriers in males (P > 0.05), whereas the female A carriers had a significantly higher ratio of LDL-C/ HDL-C compared with the female subjects with the GG genotype (P < 0.05). Following the high-carbohydrate diet, significant decreases of TC/HDL-C were found in all the groups, regardless of sex and genotype (P < 0.01). LDL-C/HDL-C experienced significant decreases in both the genotypes in males (P < 0.05), while in females, significant decrease of LDL-C/HDL-C was only observed in A carriers (P < 0.01). CONCLUSION: The A allele of the -75 G/A polymorphism in APOA1 may have specific effects on the LDL-C/HDL-C ratio in females.

[Effects of a low-fat and high-carbohydrate diet on the physiological and biochemical indices in healthy youth with different body mass index].

OBJECTIVE: To investigate the effects of a low-fat and high-carbohydrate (LF-HC) diet on the physiological and biochemical indexes in healthy youth with different body mass index (BMI). METHODS: Seven overweight participants [BMI=(27.82 +/- 1.64) kg/m2] and 49 age-matched controls [BMI = (20.06 +/- 2.41) kg/ m2] were given a washout diet for 7 d, followed by a LF-HC diet for 6 d. The washout diet contained 31.1% fat and 54.1% carbohydrate, and the LF-HC diet contained 14.8% fat and 70.1% carbohydrate of total energy. Anthropometric measurements were conducted on the mornings of the first, eighth and fourteenth days. Serum samples were prepared from twelve-hour fasting venous blood. Biochemical indexes including lipids; glucose and insulin were measured with routine methods. The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated. RESULTS: At baseline, the control group had lower levels of body mass (P = 0.000), BMI (P = 0.000), waist-hip ratio (P = 0.000), systolic blood pressure (P = 0.001), diagnostic blood pressure (P = 0.016) and triglycerides (P = 0.006), and a higher level of HDL cholesterol (P = 0.005) than the overweight group. When compared with those before the ILF-HC diet, total cholesterol (P < 0.05) and LDL cholesterol (P < 0.05) decreased, and insulin (P < 0.05) and HOMA-IR (P < 0.05) increased in both the control group and the overweight group after the LF-HC diet. Increased triglycerides (P = 0.000) were observed only in the control subjects, and HDL cholesterol (P = 0.018) increased only in the overweight subjects after the LF-HC diet. CONCLUSION: The responses of serum TG and HDL-C to the LF-HC diet are related to BMI in healthy youth.

[Effects of the LIPE C-60G polymorphism on changes of plasma lipids and glucose induced by a high-carbohydrate diet in healthy youth].

OBJECTIVE: To investigate the interaction of the C-60G polymorphism of hormone sensitive lipase gene (LIPE) with a high carbohydrate (high-CHO) diet on plasma lipids and glucose in a young and healthy Chinese Han population. METHODS: 27 males and 29 females were given a washout diets of 31% fat, 54% carbohydrate and 15% protein for 7 days, followed by the high-CHO diet of 15% fat, 70% carbohydrate and 15% protein for 6 days, without total energy restriction. Plasma lipid profiles, glucose, insulin, homeostasis model assessment for insulin resistance (HOMA-IR) and body mass index (BMI) at baseline, before and after the high-CHO diets as well as the LIPE C-60G polymorphism were analyzed. RESULTS: The females with the CC genotype had significantly higher levels of high-density lipoprotein cholesterol (HDL-C) (P < 0.01, P < 0.05) and apolipoprotein A-I (apo A-I ) (P < 0.01, P < 0.01) than the males with the same genotype both before and after the high-CHO diet. After the high-CHO diet, only the females with the CC genotype had significantly higher levels of total cholesterol (TC) (P < 0.01) and low-density lipoprotein cholesterol (LDL-C) (P < 0.05) than their male counterparts. Compared with those before the high-CHO diet, the males with the CC genotype had significantly lower levels of BMI (P < 0.05), TC (P < 0.01) and LDL-C (P < 0.01), but significantly higher levels of HDL-C (P < 0.01) and apo A-I (P < 0.05) after the high-CHO diet. The males with the CG genotype experienced significant decreases of BMI (P < 0.05) and TC (P < 0.01). The females with the CC genotype experienced significant decreases of TC (P < 0.01) and LDL-C (P < 0.01) but significant increases of triglyceride (TG) (P < 0.01) and insulin (P < 0.05). The females with the CG genotype experienced a significant decrease of TC (P < 0.05) but a significant increase of TG (P < 0.05). CONCLUSION: LIPE C-60G variation can inhibit the decrease of LDL-C and the increases of HDL-C and apo A-I in young healthy males induced by the high-CHO diet, and can inhibit the decrease of LDL-C and the increase of insulin in young healthy females induced by the same diet.

A high-carbohydrate diet enhances the adverse effect of the S2 allele of APOC3 SstI polymorphism on the TG/HDL-C ratio only in young Chinese females

Both genetic background and diet have profound effects on plasma lipid profiles. We hypothesized that a high-carbohydrate (high-CHO) diet may affect the ratios of serum lipids and apolipoproteins (apo) differently in subjects with different genotypes of the SstI polymorphism in the apoCIII gene (APOC3). Fifty-six healthy university students (27 males and 29 females, 22.89 ± 1.80 years) were given a washout diet of 54 percent carbohydrate for 7 days, followed by a high-CHO diet of 70 percent carbohydrate for 6 days without total energy restriction. Serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apoB100, apoAI, and the APOC3 SstI polymorphism were analyzed. The ratios of serum lipids and apoB100/apoAI were calculated. At baseline, the TG/HDL-C ratio was significantly higher in females, but not in males, with the S2 allele. The differences in the TG/HDL-C ratio between genotypes remained the same after the washout and the high-CHO diet in females. When compared with those before the high-CHO diet, the TC/HDL-C (male S2 carriers: 3.13 ± 1.00 vs 2.36 ± 0.65, P = 0.000; male subjects with the S1S1 genotype: 2.97 ± 0.74 vs 2.09 ± 0.55, P = 0.000; female S2 carriers: 2.68 ± 0.36 vs 2.24 ± 0.37, P = 0.004; female subjects with the S1S1 genotype: 2.69 ± 0.41 vs 2.09 ± 0.31, P = 0.000) and LDL-C/HDL-C (male S2 carriers: 1.44 ± 0.71 vs 1.06 ± 0.26, P = 0.012; male subjects with the S1S1 genotype: 1.35 ± 0.61 vs 1.01 ± 0.29, P = 0.005; female S2 carriers: 1.18 ± 0.33 vs 1.00 ± 0.18, P = 0.049; female subjects with the S1S1 genotype: 1.18 ± 0.35 vs 1.04 ± 0.19, P = 0.026) ratios were significantly decreased after the high-CHO diet regardless of gender and of genotype of the APOC3 SstI polymorphism. However, in female S2 carriers, the TG/HDL-C (1.38 ± 0.46 vs 1.63 ± 0.70, P = 0.039) ratio was significantly increased after the high-CHO diet. In conclusion, the high-CHO diet has favorable effects on the TC/HDL-C and LDL-C/HDL-C ratios regardless of gender and of genotype of the APOC3 SstI polymorphism. Somehow, it enhanced the adverse effect of the S2 allele on the TG/HDL-C ratio only in females.

A high-carbohydrate diet enhances the adverse effect of the S2 allele of APOC3 SstI polymorphism on the TG/HDL-C ratio only in young Chinese females.

Both genetic background and diet have profound effects on plasma lipid profiles. We hypothesized that a high-carbohydrate (high-CHO) diet may affect the ratios of serum lipids and apolipoproteins (apo) differently in subjects with different genotypes of the SstI polymorphism in the apoCIII gene (APOC3). Fifty-six healthy university students (27 males and 29 females, 22.89 ± 1.80 years) were given a washout diet of 54% carbohydrate for 7 days, followed by a high-CHO diet of 70% carbohydrate for 6 days without total energy restriction. Serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apoB100, apoAI, and the APOC3 SstI polymorphism were analyzed. The ratios of serum lipids and apoB100/apoAI were calculated. At baseline, the TG/HDL-C ratio was significantly higher in females, but not in males, with the S2 allele. The differences in the TG/HDL-C ratio between genotypes remained the same after the washout and the high-CHO diet in females. When compared with those before the high-CHO diet, the TC/HDL-C (male S2 carriers: 3.13 ± 1.00 vs 2.36 ± 0.65, P = 0.000; male subjects with the S1S1 genotype: 2.97 ± 0.74 vs 2.09 ± 0.55, P = 0.000; female S2 carriers: 2.68 ± 0.36 vs 2.24 ± 0.37, P = 0.004; female subjects with the S1S1 genotype: 2.69 ± 0.41 vs 2.09 ± 0.31, P = 0.000) and LDL-C/HDL-C (male S2 carriers: 1.44 ± 0.71 vs 1.06 ± 0.26, P = 0.012; male subjects with the S1S1 genotype: 1.35 ± 0.61 vs 1.01 ± 0.29, P = 0.005; female S2 carriers: 1.18 ± 0.33 vs 1.00 ± 0.18, P = 0.049; female subjects with the S1S1 genotype: 1.18 ± 0.35 vs 1.04 ± 0.19, P = 0.026) ratios were significantly decreased after the high-CHO diet regardless of gender and of genotype of the APOC3 SstI polymorphism. However, in female S2 carriers, the TG/HDL-C (1.38 ± 0.46 vs 1.63 ± 0.70, P = 0.039) ratio was significantly increased after the high-CHO diet. In conclusion, the high-CHO diet has favorable effects on the TC/HDL-C and LDL-C/HDL-C ratios regardless of gender and of genotype of the APOC3 SstI polymorphism. Somehow, it enhanced the adverse effect of the S2 allele on the TG/HDL-C ratio only in females.

Associations of the SREBP-1c gene polymorphism with gender-specific changes in serum lipids induced by a high-carbohydrate diet in healthy Chinese youth.

We investigated the possible association between the sterol regulatory element-binding protein-1c gene (SREBP-1c) rs2297508 polymorphism and the changes in lipid profiles in a high-carbohydrate and low-fat (high-CHO/LF) diet in a Chinese population well characterized by a lower incidence of coronary heart disease and a diet featuring higher carbohydrate and lower fat. Fifty-six healthy youth (aged 22.89 ± 1.80 years) were given wash-out diets of 31% fat and 54% carbohydrate for 7 days, followed by the high-CHO/LF diet of 15% fat and 70% carbohydrate for 6 days, without total energy restriction. Fasting blood samples were collected. Serum variables of lipid and glucose metabolism after the wash-out and high-CHO/LF diets, as well as the rs2297508 polymorphism, were analyzed. Compared with the male subjects on the wash-out diet, significantly elevated levels of high-density lipoprotein cholesterol (HDL-C) and decreased levels of apolipoprotein B-100 were observed in the male carriers of the C allele after the high-CHO/LF diet. In the female subjects, significantly increased triacylglycerol levels, insulin, and homeostasis model assessment of insulin resistance (HOMA-IR) were found in the GG genotype after the high-CHO/LF diet. These results suggest that the C allele of the rs2297508 polymorphism is associated with a retardation of the increases in serum triacylglycerol, serum insulin, and HOMA-IR in females and with the elevated serum HDL-C in males after the high-CHO/LF diet.

[Effects of the +83C/T polymorphism in apolipoprotein AI gene on the changes of serum biochemical traits of gluocse and lipid metabolism induced by high-carbohydrate diets].

OBJECTIVE: The aim of the present study was to investigate the possible effects of the +83C/T polymorphism in apolipoprotein AI gene (apoA1) on the changes of serum lipids, glucose, insulin and the homeostasis model assessment of insulin resistance (HOMA-IR) induced by a high-carbohydrate diet in healthy youth. METHODS: Fifty-six participants were given a washout diet for 7 d, followed by a high-carbohydrate diet for 6 d. The washout diet contained 15% protein, 31% fat and 54% carbohydrate. The high-carbohydrate diet contained 15% protein, 15% fat and 70% carbohydrate. Twelve hour fasting venous blood was drawn on the mornings of the first, eighth and fourteenth days. Blood lipids, glucose and insulin were measured, and HOMA-IR was calculated. The genome DNA was extracted and the apoA1 + 83C/T polymorphism was determined by polymerase chain reaction-based restriction fragment length polymorphism. RESULTS: Triglyceride and insulin were found significantly increased in the subjects with the CC genotype, but not in the T carriers after the high-carbohydrate diet. Significant decreases of total cholesterol and LDL-C and a significant increase of HDL-C were observed after the dietary intervention of the high-carbohydrate diet. CONCLUSION: The triglyceride and insulin changes after the high-carbohydrate diet can be modulated by the apoA1 +83C/T polymorphism, and the T allele may eliminate the increase in triglyceride and insulin induced by the high-carbohydrate diet.

[Effects of the beta2-adrenergic receptor Gln27Glu variation on changes of serum lipid and apolipoprotein ratios induced by a high-carbohydrate/low-fat diet in healthy youth].

OBJECTIVE: To investigate the effects of the Gln27Glu polymorphism of beta2-adrenergic receptor (beta2AR) on serum lipid and apolipoprotein ratios and its interaction with high-carbohydrate/low-fat (HC/LF) diet on the ratios in healthy youth. METHODS: After on a washout diet for seven days, fifty six healthy young subjects were given the HC/LF diet for six days. The 12 hour-fasting serum lipids and apolipoproteins (apo) AI and B100 were measured on the 1st, the 8th and the 14th days. The ratios of TG/HDL-C, log (TG/HDL-C), TC/HDL-C, LDL-C/HDL-C and apoAI/apoB100 were calculated. The polymorphism of Gln27Glu was analyzed by PCR-RFLP method. RESULTS: No significant differences were found of the lipid and apolipoprotein ratios at baseline and before the HC/LF diet between the subjects with the CC genotype (wide type) and the G carriers (mutation carriers) in the whole study sample or the males and the females separately. The G carriers had a significantly higher level of log (TG/HDL-C) (P=0.038) than the subjects with the CC genotype did after the HC/LF diet in the whole study sample but not in the males and the females separately. Significant decreases of LDL-C/HDL-C and TC/HDL-C were observed in all the subjects after the HC/LF diet when compared with those before the HC/LF diet (P<0.05), but a significant increase of apoAI/apoB100 (P=0.021) only in the subjects with the CC genotype. When gender was taken into account, significant decreases of LDL-C/HDL-C and TC/HDL-C were found after the HC/ LF diet in the male subjects regardless of genotypes (P<0.05). Significant increases of TG/HDL-C and log (TG/ HDL-C) and a significant decrease of TC/HDL-C were found in all the female subjects (P<0.05), while a significant decrease of LDL-C/HDL-C (P=0.037) was only observed in the female subjects with the CC genotype. CONCLUSION: The G allele of beta2AR Gln27Glu variation can inhibit the decrease of LDL-C/HDL-C in females after HC/LF diet intervention.

[Effects of Pvu II polymorphism in low density lipoprotein receptor gene on changes of serum lipid ratios induced by high-carbohydrate/low-fat diet in healthy youth].

OBJECTIVE: To investigate the effects of low density lipoprotein receptor gene (LDLR) Pvu II polymorphism on serum lipid ratios and their responses to high-carbohydrate/low-fat (HC/LF) diet in healthy youth. METHODS: After a regular diet for 7 days of wash-out, 56 health youth ((22.89 +/- 1.80) years old)] were given LF/HC diet for 6 days. The regular diet contained 54% carbohydrate, 15% protein, and 31% fat of the total energy. The LF/HC diet contained 70% carbohydrate, 15% proteine and 15% fat of the total energy. The serum lipids were measured on the 1st, the 8th and the 14th days. The ratios of TG/HDL-C, log (TG/HDL-C), TC/HDL-C, and LDL-C/HDL-C were calculated. The polymorphism of LDLR was detected by PCR-RFLP method and its relationship with serum lipid ratios was analyzed. RESULTS: No significant difference was found of the lipid ratios at baseline in subjects with different genotypes in both genders. Before or after HC/LF diet, no significant difference was found of the lipid ratios between the subjects with different genotypes in the whole study population or in males and females separately. Compared with those of before HC/LF diet, all the subjects experienced significant decrease of TC/HDL-C and LDL-C/HDL-C (P < 0.05). When gender was taken into account, significant increases of TG/HDL-C and log(TG/HDL-C) were found only in females (P < 0.05). All the subjects experienced significant decrease of TC/HDL-C regardless of gender and genotype (P < 0.05). As for LDL-C/HDL-C, significant decrease was found only in males with P1P1 and female subjects (P < 0.05). CONCLUSION: Polymorphism of LDLR Pvu II may influence the response of LDL-C/HDL-C to HC/LF diet in males.

[Effects of the 54G/C polymorphism of sterol regulatory element-binding protein-1c on changes of serum lipid ratios induced by high-carbohydrate/low-fat diet in healthy youth].

OBJECTIVE: To investigate the effects of 54G/C polymorphism of sterol regulatory element-binding protein-1c gene (SREBP-1c) on serum lipid ratios and their response to high-carbohydrate/low-fat (HC/LF) diet in healthy youth. METHODS: After a regular diet for 7 days of wash-out, 56 healthy youth (22.89 +/- 1.80 yrs) were given HC/LF diet for 6 days. The regular diet contained 54% carbohydrate, 15% protein, and 31% fat of the total energy. The HC/LF diet contained 70% carbohydrate, 15% protein, and 15% fat of the total energy. The serum lipids and glucose were measured on the 1st, 8th and 14th days. The ratios of TG/HDL-C, log (TG/HDL-C), TC/HDL-C, and LDL-C/HDL-C were calculated. The 54G/C polymorphism of SREBP-1c gene was analyzed by PCR-RFLP method. RESULTS: No significant difference was found in lipid ratios and glucose at baseline and after regular diet in subjects with different genotypes in either the whole studied population or in males or females only. However, after HC/LF diet, LDL-C/HDL-C was significantly lower in females carrying the C allele than those of GG homozygotes (P< 0.05). Compared with those before HC/LF diet, TC/HDL-C and LDL-C/HDL-C were significantly decreased in all the subjects (P< 0.05). When gender was taken into account, significant increase of TG/HDL-C and log(TG/HDL-C) was found only in females with GG genotype (P< 0.05). All the subjects experienced significant decrease of TC/HDL-C and LDL-C/HDL-C regardless of their genders and genotypes (P< 0.05). CONCLUSION: The 54G/C polymorphism of SREBP-1c gene can influence the response of TG/HDL-C and log(TG/HDL-C) to HC/LF diet in females. The C allele may be a protective factor to prevent the increase of TG induced by HC/LF diet in females.

[Effects of adiponectin gene SNP45T/G on changes of serum lipid ratios induced by high-carbohydrate/low-fat diet in healthy Chinese youth].

OBJECTIVE: To investigate the effects of adiponectin gene (APM1) SNP45T/G on serum lipid ratios and their responses to high-carbohydrate/low-fat (HC/LF) diet in healthy young Chinese. METHODS: Fifty-six healthy young subjects were given two consecutive diets. The first was control diet (54% carbohydrate, 15% protein, and 31% fat) for 7 days, and the second was HC/LF diet (70% carbohydrate, 15% protein, and 15% fat) for 6 days. Before and after each diet, serum lipids and SNP45T/G were analyzed. The ratios of TG/HDL-C, log (TG/HDL-C), TC/HDL-C, and LDL-C/HDL-C were calculated. RESULTS: There was no significant difference of baseline lipid ratios between subjects with TT genotype and subjects carrying G allele (G carriers) in the whole population or in the males and females separately. The G allele was associated with significantly higher TC/HDL-C after HC/LF diet in the males (P < 0.05); and the males with TT genotype had significant decreases of LDL-C/HDL-C (P < 0.05) and TC/HDL-C (P < 0.05) after HC/LF diet compared with those before the diet, while G carriers only experienced significant decrease of TC/HDL-C (P < 0.01). In the females, TT genotype was associated with significantly higher TG/HDL-C (P < 0.05) and log (TG/HDL-C) (P < 0.05) both before and after the HC/LF diet. When compared with those before HC/LF diet, elevated TG/HDL-C (P < 0.05) and log (TG/ HDL-C) (P < 0.05) and declined TC/HDL-C (P < 0.01) were observed in the subjects with TT genotype after the diet. In the female subjects of G carriers, LDL-C/HDL-C (P < 0.05) and TC/HDL-C (P < 0.01) decreased significantly after the HC/LF diet. CONCLUSION: G allele of APM1 45T/G could inhibit increase of TG/HDL-C and log (TG/HDL-C) and promote the decrease of LDL-C/HDL-C induced by HC/LF diet in healthy young females. But in the healthy young males, it might eliminate the decline of LDL-C/HDL-C induced by HC/LF diet and increase TC/HDL-C.

[Effects of cholesterol ester transfer protein taqIB polymorphism on changes of serum lipid ratios induced by high-carbohydrate/low-fat diet in healthy youth].

OBJECTIVE: To investigate the effects of cholesterol ester transfer protein gene (CETP) TaqIB polymorphism on serum lipid ratios and their responses to high-carbohydrate/low-fat (HC/LF) diet in healthy youth. METHODS: After on regular diet for 7 days, 56 health youth [(22.89+/-1.80) years] were given HC/LF diet for 6 days. The regular diet contained 54% carbohydrate, 15% protein, and 31% fat of the total energy. The LF/HC diet contained 70% carbohydrate, 15% protein, and 15% fat of the total energy. The serum lipids were measured on the 1st, the 8th and the 14th day. The ratios of TG/HDL-C, log (TG/HDL-C), TC/HDL-C, and LDL-C/HDL-C were calculated. The polymorphism of CETP was analyzed by PCR-RFLP method. RESULTS: No significant difference was found of the lipid ratios at baseline in subjects with different genotypes in both genders. After the regular diet, TC/HDL-C was significantly lower in males carrying the B2 allele than B1B1 homozygotes (P<0.05). After HC/LF diet, no significant difference was found of the lipid ratios between the subjects with different genotypes in the whole study population or in males and females separately. Compared with those before HC/LF diet, subjects with B1B1 genotype experienced significant changes of the four lipid ratios after HC/LF diet (P<0.05), while the B2 carriers had only significant decrease of TC/HDL-C and LDL-C/HDL-C (P<0.05). No significant difference of the changes of the four lipid ratios before and after HC/LF diet was found between the subjects with different genotypes. When gender was taken into account, significant increases of TG/HDL-C and log (TG/HDL-C) were found in females with B1B1 genotype (P<0.05). All the subjects experienced significant decrease of TC/HDL-C and LDL-C/HDL-C regardless of gender and genotype (P<0.05). As for the changes of lipid ratios before and after HC/LF diet, difference of only TC/HDL-C was found between males with B1B1 and male carriers of B2 (P<0.05). CONCLUSION: Polymorphism of CETP TaqIB may influence the response of TC/HDL-C to HC/LF diet in males and the increases of TG/HDL-C and log (TG/HDL-C) induced by HC/LF diet in females.

No decrease of HDL cholesterol after 6 days of low fat and high carbohydrate diets in a young Chinese Han population.

More studies are needed on the hypertriacylglycerolemic effects of low fat and high carbohydrate (LF-HC) diet in young population, especially Chinese who generally have a diet containing lower fat and higher carbohydrate. To test them in a young Chinese Han population, 56 healthy subjects (22.89 +/- 1.80) years were given regular diet of 31% fat and 54% carbohydrate for 7 days, followed by LF-HC diet of 15% fat and 70% carbohydrate for 6 days, without total energy restriction. After the LF-HC diet, the male experienced an increase of high density lipoprotein (HDL) cholesterol and decreases of weight, body mass index (BMI), total cholesterol (TC), and LDL (low density lipoprotein) cholesterol (P < 0.05). The female experienced increased serum triacylglycerol and insulin, and decreased TC and LDL cholesterol (P < 0.05). When BMI was taken into account, all the subjects with low, medium, or high BMI experienced decreases of TC and LDL cholesterol although some changes were not significant. No significant decrease of HDL cholesterol was found, while significantly increased HDL cholesterol and apolipoprotein A-I (apo A-I) were found in the male subjects with low or high BMI (P < 0.05). Significant increase of triacylglycerol was observed only in the female subjects with low or medium BMI. In conclusion, subjects with different BMI and gender have different triacylglycerol and HDL cholesterol responses to LF-HC diets, and significant increase of HDL cholesterol and apolipoprotein A- I were observed in some young male subjects.