RESUMO
Upper respiratory tract is the primary site of SARS-CoV-2 replication. Releasing of pro and anti-inflammatory mediators plays an important role in the immunopathogenesis of Coronavirus Disease 2019 (COVID-19). The aim of this study was to evaluate the early inflammatory response in upper airway by measuring of IFN-γ, TGF-ß1 and RANTES at mRNA level. Forty five SARS-CoV-2 infected patients were enrolled, whose were divided in two groups: asymptomatic and symptomatic. Twenty healthy persons, SARS-CoV-2 negative were included as controls. Higher IFN-γ expression was detected in SARS-CoV-2 infected patients in comparison with controls (p = 0.0393). IFN-γ expression was increased in symptomatic patients (p = 0.0405). TGF-ß1 and RANTES expressions were lower in SARS-CoV-2 infected patients than controls (p < 0.0001; p = 0.0011, respectively). A significant correlation between IFN-γ and TGF-ß1 was observed in SARS-CoV-2 asymptomatic patients (r = +0.61, p = 0.0014). The findings suggest that imbalance between IFN-γ and TGF-ß1 expression could be an impact in clinical expression of SARS-CoV-2 infection.
Assuntos
Betacoronavirus/patogenicidade , Quimiocina CCL5/genética , Infecções por Coronavirus/imunologia , Interferon gama/genética , Pneumonia Viral/imunologia , RNA Mensageiro/genética , Fator de Crescimento Transformador beta1/genética , Adulto , Doenças Assintomáticas , Betacoronavirus/imunologia , COVID-19 , Estudos de Casos e Controles , Quimiocina CCL5/imunologia , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/patologia , Infecções por Coronavirus/virologia , Feminino , Expressão Gênica , Interações Hospedeiro-Patógeno/genética , Interações Hospedeiro-Patógeno/imunologia , Humanos , Interferon gama/imunologia , Pulmão/imunologia , Pulmão/patologia , Pulmão/virologia , Masculino , Pessoa de Meia-Idade , Nasofaringe/imunologia , Nasofaringe/patologia , Nasofaringe/virologia , Pandemias , Pneumonia Viral/diagnóstico , Pneumonia Viral/patologia , Pneumonia Viral/virologia , RNA Mensageiro/imunologia , SARS-CoV-2 , Índice de Gravidade de Doença , Fator de Crescimento Transformador beta1/imunologiaRESUMO
Se estudió la presencia de anticuerpos contra los virus Parainfluenza humanos tipos 2 y 3 en 1 793 sueros de una población infantil menor de 14 años de edad. Para el pesquisaje de las muestras clínicas se empleó como sistema la técnica de inhibición de la hemaglutinación. Del total de sueros analizados fueron positivos 1 382 (77,1 por ciento), de estos se confirmó la presencia de anticuerpos contra el tipo 2 en 320 (17,8 por ciento), para el tipo 3 en 334 (18,6 por ciento) y predominó la seropositividad simultánea en 805 (44,9 por ciento). Se constató la circulación de los virus Parainfluenza humanos durante todos los meses del año y en todos los grupos de edades analizados, con aumento en los porcentajes de positividad con la edad
Assuntos
Humanos , Criança , Hemaglutinação por Vírus , Vírus da Parainfluenza 2 Humana , Vírus da Parainfluenza 3 HumanaRESUMO
The presence of antibodies against human parainfluenza viruses type 2 and 3 was studied in 1 793 sera from an infantile population under 14 years old. The hemagglutination inhibition technique was used for screening the clinical samples. Of the total of analyzed sera, 1 382 (77.1%) were positive. The presence of antibodies against type 2 was confirmed in 320 of them (17.8%), and against type 3 in 334 (18.6%). The simultaneous seropositivity predominated in 805 (44.9%). The circulation of human parainfluenza viruses was corroborated during all the months of the year and in all the analyzed age groups. It was observed an increase in the positivity percentages with age.