Epidemiological surveillance of ovine hydatidosis in Tierra del Fuego, Patagonia Argentina, 1997-1999.

Cystic echinococcosis is the most prevalent zoonosis in Tierra del Fuego province, Argentina, with important economic, productive and public health consequences. The present work was performed to determine the ovine prevalence in Tierra del Fuego, Argentina, as well as to evaluate the quality of diagnostic systems in slaughterhouses. Moreover, genetic analyses to characterize the strain of Echinococcus granulosus involved in the region were done. The first actions to perform a diagnosis of the epidemiological situation of hydatidosis in Tierra del Fuego were done between 1976 and 1977. A canine prevalence of 80% and an ovine prevalence of 55% results were obtained. Since 1979 the control program of Hydatidosis of Tierra del Fuego was implemented. It was based on semiannual canine anthelmintic treatment with praziquantel at dose of 5mg/kg, and complemented with sanitary education and canine and ovine epidemiological surveillance. During May 1997-January 1999: 5,916 sheep coming from 20 farms of the programmatic area were evaluated. In the lamb category, hydatid cysts were not found. In the adults category, 62 infected animals were found (3.2%). The ovine prevalence was 1.1% and there was 100% of coincidence between diagnosis in the slaughterhouse, re-inspection in the laboratory and histopathological study. The marked decrease in the prevalence observed for sheep infection evidenced a destabilization of the biological cycle of the parasite. This could be explained by the application of a control program with uninterrupted systematic actions. Polymerase chain reaction-ribosomal ITS-1 DNA (rDNA) restriction fragment length polymorphism (PCR-RFLP) analysis and partial sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene were used to characterize E. granulosus isolates collected from different regions of Tierra del Fuego to determine which genotypes occurred in this region. The results revealed the presence of the G1 genotype (sheep-dog strain). This is the first time that a molecular analysis was performed for the E. granulosus isolates from Tierra del Fuego.

Diagnóstico de la hidatidosis mediante la prueba de doble difusión arco 5 en ciudadanos de la clase 1964 de la Provincia del Chubut / Diagnosis of hydatidosis by means of double dyfusion test arc 5 in students of the 1964 class of the Province of Chubut

Se determinó el número de portadores de quistes hidatídicos, y su procedencia, en una muestra selecionada de ciudadanos próximos a ser incorporados al servicio militar en la Provincia del Chubut. El examen de los sueros mediante la prueba de doble difusión arco 5 permitió establecer que el 2.27 por ciento de ellos se encontraba afectado por esta patología. Los hallazgos demuestran la unidad de la encuestas inmunodiagnósticas para la detección precoz de casos y la vigilancia epidemiológica de los programas de control