RESUMO
Using a combination of DNA and hybrid DNA-RNA substrates, we have analyzed the mechanism of phosphoryl transfer by the Flp site-specific recombinase in three different reactions: DNA strand breakage and joining, and two types of RNA cleavage activities. These reactions were then used to characterize Flp variants altered at His309 and His345, amino acid residues that are in close proximity to two key catalytic residues (Arg308 and Tyr343). These histidine residues are important for strand cutting by Tyr343, the active-site nucleophile of Flp, but neither residue contributes to the type II RNA cleavage activity or to the strand-joining reaction in a pre-cleaved substrate. Strand cleavage reactions using small, diffusible nucleophiles indicate that this histidine pair contributes to the correct positioning and activation of Tyr343 within the shared active site of Flp. The implications of these results are evaluated against the recently solved crystal structure of Flp in association with a Holliday junction.
Assuntos
DNA Nucleotidiltransferases/química , DNA Nucleotidiltransferases/metabolismo , Histidina/metabolismo , RNA/metabolismo , Recombinação Genética/genética , Saccharomyces cerevisiae/enzimologia , Substituição de Aminoácidos/genética , Sítios de Ligação , Catálise , DNA/química , DNA/genética , DNA/metabolismo , DNA Nucleotidiltransferases/classificação , DNA Nucleotidiltransferases/genética , Teste de Complementação Genética , Histidina/genética , Peróxido de Hidrogênio/metabolismo , Conformação de Ácido Nucleico , Ácidos Nucleicos Heteroduplexes/química , Ácidos Nucleicos Heteroduplexes/genética , Ácidos Nucleicos Heteroduplexes/metabolismo , Mutação Puntual/genética , RNA/química , RNA/genética , Ribonucleases/química , Ribonucleases/classificação , Ribonucleases/genética , Ribonucleases/metabolismo , Tiramina/metabolismoRESUMO
In this study, we have used multiple strategies to characterize the mechanisms of the type I and type II RNA cleavage activities harbored by the Flp (pronounced here as "flip") site-specific DNA recombinase (Flp-RNase I and II, respectively). Reactions using half-sites pre-bound by step-arrest mutants of Flp agree with a "shared active site" being responsible for the type I reaction (as is the case with normal DNA recombination). In a "pre-cleaved" type I substrate containing a 3'-phosphotyrosyl bond, the Flp-RNase I activity can be elicited by either wild type Flp or by Flp(Y343F). Kinetic analyses of the type I reaction are consistent with the above observations and support the notion that the DNA recombinase and type I RNase active sites are identical. The type II RNase activity is expressed by Flp(Y343F) in a half-site substrate and is unaffected by the catalytic constitution of a Flp monomer present on a partner half-site. Reaction conditions that proscribe the assembly of a DNA bound Flp dimer have no effect on Flp-RNase II. These biochemical results, together with kinetic data, are consistent with the reaction being performed from a "non-shared active site" contained within a single Flp monomer. The Flp-related recombinase Cre, which utilizes a non-shared recombination active site, exhibits the type I RNA cleavage reaction. So far, we have failed to detect the type II RNase activity in Cre. Despite their differences in active site assembly, Cre functionally mimics Flp in being able to provide two functional active sites from a trimer of Cre bound to a three-armed (Y-shaped) substrate.
Assuntos
DNA Nucleotidiltransferases/metabolismo , Integrases/metabolismo , Ribonucleases/metabolismo , Tirosina/metabolismo , Sequência de Bases , Sítios de Ligação , Catálise , DNA Nucleotidiltransferases/genética , Cinética , Mutagênese , Oligorribonucleotídeos , Tiramina/metabolismoRESUMO
Recombinases of the lambda-Int family and type IB topoisomerases act by introducing transient single strand breaks in DNA using chemically identical reaction schemes. Recent structural data have supported the relationship between the two enzyme groups by revealing considerable similarities in the architecture of their catalytic pockets. In this study we show that the Int-type recombinase Flp is inhibited by the two structurally unrelated topoisomerase I-directed anti-cancer drugs, camptothecin (CPT) and NSC-314622. The interaction of these drugs with topoisomerase I is very specific with several single amino acid substitutions conferring drug resistance to the enzyme. Thus, the observed interaction of CPT and NSC-314622 with Flp, which is comparable to their interaction with the cleavage complex formed by topoisomerase I, strongly supports a close mechanistic and evolutionary relationship between the two enzymes. The results suggest that Flp and other Int family recombinases may provide model systems for dissecting the molecular mechanisms of topoisomerase I-directed anti-cancer therapeutic agents.
Assuntos
Antineoplásicos/farmacologia , Camptotecina/farmacologia , DNA Nucleotidiltransferases/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Indenos/farmacologia , Isoquinolinas/farmacologia , Inibidores da Topoisomerase I , Sítios de Ligação , Ligação Competitiva , DNA/efeitos dos fármacos , Dano ao DNA , Relação Dose-Resposta a Droga , Evolução Molecular , Humanos , Cinética , Modelos Biológicos , Ligação ProteicaRESUMO
The Flp site-specific recombinase functions in the copy number amplification of the yeast 2 microm plasmid. The recombination reaction is catalyzed by four monomers of Flp bound to two separate, but identical, recombination sites (FRT sites) and occurs in two sequential pairs of strand exchanges. The relative orientation of the two recombination sites during synapsis was examined. Topoisomerase relaxation and nick ligation were used to detect topological nodes introduced by the synapse prior to the chemical steps of recombination. A single negative supercoil was found to be trapped by Flp in substrates with inverted FRT sites whereas no trapped supercoils were observed with direct repeats. The topology of products resulting from Flp-mediated recombination adjacent to a well characterised synapse, that of Tn3 resolvase/res, was analyzed. The deletion and inversion reactions yielded the four noded catenane and the three noded knot, respectively, as the simplest and the most abundant products. The linking number change introduced by the Flp-mediated inversion reaction was determined to be +/-2. The most parsimonious explanation of these results is that Flp aligns its recombination sites with antiparallel geometry. The majority of synapses appear to occur without entrapment of additional random plectonemic DNA supercoils between the sites and no additional crossings are introduced as a result of the chemical steps of recombination.
Assuntos
DNA Nucleotidiltransferases/metabolismo , Integrases/metabolismo , Família Multigênica , Recombinação Genética/genética , Saccharomyces/enzimologia , Saccharomyces/genética , Transposon Resolvases , Proteínas Virais , Sítios de Ligação , Inversão Cromossômica , Troca Genética/genética , DNA Ligases/metabolismo , DNA Topoisomerases Tipo I/metabolismo , DNA Topoisomerases Tipo II/metabolismo , DNA Super-Helicoidal/química , DNA Super-Helicoidal/genética , DNA Super-Helicoidal/metabolismo , Desoxirribonuclease I/metabolismo , Humanos , Modelos Genéticos , Conformação de Ácido Nucleico , Plasmídeos/química , Plasmídeos/genética , Plasmídeos/metabolismo , Recombinases , Deleção de Sequência/genética , Especificidade por Substrato , Transposases/metabolismoRESUMO
The integrase family of site-specific recombinases (also called the tyrosine recombinases) mediate a wide range of biological outcomes by the sequential exchange of two pairs of DNA strands at defined phosphodiester positions. The reaction produces a recombinant arrangement of the DNA sequences flanking the cross-over region. The crystal structures of four integrase family members have revealed very similar three-dimensional protein folds that belie the large diversity in amino acid sequences among them. The active sites are similar in organization to those seen in structures of eukaryotic type IB topoisomerases, and conservation of catalytic mechanism is expected. The crystal structures, combined with previous biochemical knowledge, allow the refinement of models for recombination and the assignment of catalytic function to the active site residues. However, each system has its own peculiarities, and the exact sequence of events that allows the reaction to proceed from the first exchange reaction to the second is still unclear for at least some family members.
Assuntos
DNA Nucleotidiltransferases/química , Integrases/química , Recombinação Genética , Sítios de Ligação , DNA Topoisomerases Tipo I/química , Humanos , Modelos Moleculares , Conformação de Ácido Nucleico , Conformação Proteica , RecombinasesRESUMO
Xer site-specific recombination functions in the stable maintenance of circular replicons in Escherichia coli. Each of two related recombinase proteins, XerC and XerD, cleaves a specific pair of DNA strands, exchanges them, and rejoins them to the partner DNA molecule during a complete recombination reaction. The rejoining activity of recombinase XerC has been analyzed using isolated covalent XerC-DNA complexes resulting from DNA cleavage reactions upon Holliday junction substrates. These covalent protein-DNA complexes are competent in the rejoining reaction, demonstrating that covalently bound XerC can catalyze strand rejoining in the absence of other proteins. This contrasts with a recombinase-mediated cleavage reaction, which requires the presence of both recombinases, the recombinase mediating catalysis at any given time requiring activation by the partner recombinase. In a recombining nucleoprotein complex, both cleavage and rejoining can occur prior to dissociation of the complex.
Assuntos
DNA Nucleotidiltransferases/genética , DNA Bacteriano/genética , Proteínas de Escherichia coli , Escherichia coli/genética , Integrases , Recombinação Genética , Sequência de Bases , Dados de Sequência Molecular , RecombinasesRESUMO
Site-specific recombinases of the Integrase family utilize a common chemical mechanism to break DNA strands during recombination. A conserved Arg-His-Arg triad activates the scissile phosphodiester bond, and an active-site tyrosine provides the nucleophile to effect DNA cleavage. Is the tyrosine residue for the cleavage event derived from the same recombinase monomer which provides the RHR triad (DNA cleavage in cis), or are the triad and tyrosine derived from two separate monomers (cleavage in trans)? Do all members of the family follow the same cleavage rule, cis or trans? Solution studies and available structural data have provided conflicting answers. Experimental results with the Flp recombinase which strongly support trans cleavage have been derived either by pairing two catalytic mutants of Flp or by pairing wild-type Flp and a catalytic mutant. The inclusion of the mutant has raised new concerns, especially because of the apparent contradictions in their cleavage modes posed by other Int family members. Here we test the cleavage mode of Flp using an experimental design which excludes the use of the mutant protein, and show that the outcome is still only trans DNA cleavage.
Assuntos
DNA Nucleotidiltransferases/metabolismo , DNA Viral/metabolismo , Recombinação Genética , Sequência de Aminoácidos , Bacteriófago lambda/genética , Bacteriófago lambda/metabolismo , Sequência de Bases , Domínio Catalítico/genética , Sequência Conservada , DNA Nucleotidiltransferases/química , DNA Nucleotidiltransferases/genética , DNA Viral/genética , Dimerização , Integrases/metabolismo , Conformação Proteica , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismoRESUMO
The site-specific DNA recombinase Flp shows two types of RNA cleavage activities on hybrid DNA-RNA substrates. One targets the phosphodiester position involved in DNA recombination and follows a related mechanistic path. In this two-step reaction, first-strand scission is mediated by a nucleophilic attack of the scissile phosphodiester bond by the active site tyrosine of Flp. The resultant 3'-O-phosphoryl tyrosine bond is then attacked by the adjacent 2'-hydroxyl group. The second activity targets the immediately adjacent phosphodiester bond to the 3' side using a distinct mechanism. In this reaction, the vicinal 2'-hydroxyl directly attacks the phosphate group in a manner that is reminiscent of the pancreatic RNase mechanism. The Flp protein can also be shown to possess a topoisomerase-like activity.
Assuntos
DNA Nucleotidiltransferases/metabolismo , DNA Topoisomerases Tipo I/metabolismo , Ribonucleases/metabolismo , Sequência de Bases , Proteínas Fúngicas/metabolismo , Hibridização de Ácido Nucleico , RNA Fúngico/metabolismo , Saccharomyces cerevisiae/enzimologia , Saccharomyces cerevisiae/genéticaRESUMO
In Xer site-specific recombination, two related recombinases, XerC and XerD, mediate the formation of recombinant products using Holliday junction-containing DNA molecules as reaction intermediates. Each recombinase catalyses the exchange of one pair of specific strands. By using synthetic Holliday junction-containing recombination substrates in which two of the four arms are tethered in an antiparallel configuration by a nine thymine oligonucleotide, we show that XerD catalyses efficient strand exchange only when its substrate strands are 'crossed'. XerC also catalyses very efficient strand exchange when its substrate strands are 'crossed', though it also appears to be able to mediate strand exchange when its substrate strands are 'continuous'. By using chemical probes of Holliday junction structure in the presence and absence of bound recombinases, we show that recombinase binding induces unstacking of the bases in the centre of the recombination site, indicating that the junction branch point is positioned there and is distorted as a consequence of recombinase binding.
Assuntos
Proteínas de Bactérias , DNA Nucleotidiltransferases/metabolismo , DNA Bacteriano/metabolismo , Proteínas de Escherichia coli , Integrases , Conformação de Ácido Nucleico , Sítios de Ligação , Catálise , DNA Bacteriano/química , Desoxirribonuclease BamHI/metabolismo , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Ácido Edético/farmacologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/enzimologia , Escherichia coli/genética , Compostos Ferrosos/farmacologia , Radical Hidroxila , Tetróxido de Ósmio/farmacologia , Oxidantes/farmacologia , Permanganato de Potássio/farmacologia , Proteínas , Recombinases , Especificidade por Substrato , TiminaRESUMO
Site-specific recombination mediated by XerC and XerD functions in the segregation of circular replicons in Escherichia coli. A key feature of most models of recombination for the family of recombinases to which XerC and XerD belong is that a Holliday junction forms at the position of the first pair of recombinase-mediated strand exchanges and then branch migrates 6-8 bp to the position of the second pair of strand exchanges. We have tested this hypothesis for Xer recombination by studying the effects of junction position on XerC-mediated strand exchange in vitro. Recombination of synthetic Holliday junction substrates in which junction mobility was constrained to a region extending over or removed away from the normal cleavage and exchange point was analysed. All substrates undergo strand cleavage at the normal position. We infer that the Holliday junction need not be at this position during strand cleavage and exchange. With substrates in which the Holliday junction is constrained to a region away from the XerC-mediated cleavage point, strand exchange generates products with the predicted mispaired bases.