RESUMO
BACKGROUND: Several studies have attempted to characterise the origin of canine transmissible venereal tumour (CTVT). However, the participation of cancer stem cells (CSC) has not been reported OBJECTIVES: Herein we describe the expression patterns of CSC markers CD44, CD34, CD90 and CD133 in CTVT METHODS: Thirty-eight samples were selected and assessed through flow cytometry and immunohistochemistry RESULTS AND CONCLUSIONS: Twenty-two tumours were classified as plasmacytoid and 16 as mixed. Almost all tumours showed high CD44 and low CD34 levels. CD133 and CD90 expression varied among tumours. Cytological groups did not differ in the proportion of CSC markers. Our results suggest that CSC subpopulations might participate in CTVT.
Assuntos
Doenças do Cão , Neoplasias , Tumores Venéreos Veterinários , Cães , Animais , Doenças do Cão/metabolismo , Neoplasias/veterinária , Imuno-Histoquímica , Células-Tronco Neoplásicas/metabolismo , Células-Tronco Neoplásicas/patologiaRESUMO
Mast cell tumors (MCTs) are hematopoietic neoplasms composed of mast cells. It is highly common in dogs and is extremely important in the veterinary oncology field. It represents the third most common tumor subtype, and is the most common malignant skin tumor in dogs, corresponding to 11% of skin cancer cases. The objective of this critical review was to present the report of the 2nd Consensus meeting on the Diagnosis, Prognosis, and Treatment of Canine Cutaneous and Subcutaneous Mast Cell Tumors, which was organized by the Brazilian Association of Veterinary Oncology (ABROVET) in August 2021. The most recent information on cutaneous and subcutaneous mast cell tumors in dogs is presented and discussed.
Assuntos
Doenças do Cão , Transtornos Mieloproliferativos , Neoplasias Cutâneas , Animais , Doenças do Cão/diagnóstico , Doenças do Cão/terapia , Cães , Mastócitos/patologia , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/terapia , Neoplasias Cutâneas/veterinária , Tela Subcutânea/patologiaRESUMO
The feline inductive odontogenic tumor (FIOT) is a rare entity among oral tumors in cats, the cytologic features of which are not well characterized but may prove useful. A fine-needle aspiration biopsy was performed on the right mandible between the permanent canine (404) and permanent fourth premolar (408) of an 8-month-old female mongrel cat. Cytologic smears showed epithelial cells and occasional spindle cells with extracellular homogenous acidophilic material in a hematic background. The initial diagnosis of benign tumor was confirmed by histopathologic examination of the biopsy specimen. This report describes the cytologic findings in a case of FIOT.
Assuntos
Doenças do Gato/patologia , Neoplasias Mandibulares/veterinária , Tumores Odontogênicos/veterinária , Animais , Biópsia por Agulha Fina/veterinária , Gatos , Feminino , Neoplasias Mandibulares/patologia , Tumores Odontogênicos/patologiaRESUMO
BACKGROUND: Follicular lipidosis is a rare disease, having only been reported in six Rottweiler dogs. The diagnosis of follicular lipidosis is confirmed by histopathological examination of the affected skin. OBJECTIVE: To report a case of follicular lipidosis in a dachshund dog. ANIMALS: A 13-month-old female dachshund dog, with hypotrichosis on the head and cervical region from 10 months of age. METHODS: Histological examination of skin biopsy specimens. RESULTS: Histological examination revealed basket weave orthokeratosis, ballooning of matrix cells and external root sheath keratinocytes. CLINICAL IMPORTANCE: This case report highlights the importance of including follicular lipidosis among the differential diagnoses of noninflammatory and nonhormonal cutaneous lesions in dachshund presented with hair loss.
Assuntos
Doenças do Cão/diagnóstico , Lipidoses/veterinária , Dermatopatias/veterinária , Animais , Diagnóstico Diferencial , Doenças do Cão/patologia , Cães , Feminino , Hipotricose/diagnóstico , Hipotricose/patologia , Hipotricose/veterinária , Lipidoses/diagnóstico , Lipidoses/patologia , Pele/patologia , Dermatopatias/diagnóstico , Dermatopatias/patologiaRESUMO
Type I collagen is the main dermal component, and its evaluation is relevant to quantitative studies in dermatopathology. However, visual gradation (0 to 4+) has low precision and high subjectivity levels. This study aimed to develop and validate a digital morphometric analysis technique to estimate type I collagen levels in the papillary dermis. Four evaluators visually quantified (0 to 4+) the density of type I collagen in 63 images of forearm skin biopsies marked by immunohistochemistry and two evaluators analyzed the same images using digital morphometric techniques (RGB split colors (I) and color deconvolution (II)). Automated type I collagen density estimation in the papillary dermis (two techniques) were correlated with visual evaluations (Spearman's rho coefficients of 0.48 and 0.62 (p<0.01)). With regard to the inter-observer repeatability, the four evaluators who used visual classification had an intraclass correlation coefficient (for absolute agreement) of 0.53, while the other two evaluators who used digital analysis (algorithm II) had an intraclass correlation coefficient of 0.97.
Assuntos
Algoritmos , Colágeno Tipo I/análise , Derme/anatomia & histologia , Processamento de Imagem Assistida por Computador/métodos , Idoso , Biópsia , Humanos , Interpretação de Imagem Assistida por Computador , Imuno-Histoquímica , Pessoa de Meia-Idade , Variações Dependentes do Observador , Reprodutibilidade dos Testes , Coloração e Rotulagem/métodos , Estatísticas não ParamétricasRESUMO
CASE SUMMARY: We describe the case of a 1-year-old male Persian cat diagnosed with congenital hepatic fibrosis (CHF) associated with renal polycystic disease and, for the first time, we have shown that there was no C >A mutation in exon 29 of PKD1 (polycystic kidney disease 1). The cat presented with a history of chronic weight loss, anorexia, vomiting, depression and lethargy, with profuse salivation and ascites on clinical examination. A mild elevation in liver-associated plasma enzymes suggested a hepatic disease. Owing to the cat's deteriorating condition, it was euthanized. During necropsy, the liver was found to be enlarged, firm and reddish, and the kidney had multiple small cortical cysts. Immunohistochemistry revealed that bile duct cells and epithelial cells of renal cysts showed positive immunoreactivity to keratin 19. Collagen fibers surrounding bile ducts within portal areas demonstrated reactivity to type IV collagen antibody, confirming the congenital nature of the process. A diagnosis of ductal plate malformation consistent with CHF associated with polycystic kidney in a young Persian cat was made. Interestingly, genetic testing revealed a wild-type sequence at position 3284 in exon 29 of PKD1. RELEVANCE AND NOVEL INFORMATION: The absence of the classic genetic mutation associated with the particular clinical presentation supports the hypothesis of a distinct etiopathogenesis among fibropolycystic diseases in domestic cats. Moreover, congenital hepatic fibrosis is a rare but important differential diagnosis for young Persian cats and their crosses with clinical signs of chronic end-stage liver disease.
RESUMO
BACKGROUND: Melanoma is one of the most common skin neoplasms in humans and dogs. The tumor microenvironment in melanoma comprises cancer cells and stromal cells that interact to accelerate tumor progression. Several prognostic markers for melanomas have been studied in many human tumors, including fibroblast-specific protein 1 (S100A4). S100A4 is a member of the S100 family of calcium-binding proteins in stromal cells. HYPOTHESIS/OBJECTIVES: The objective of this study was to describe the immunohistochemical patterns of S100A4 in stroma and neoplastic cells of canine skin melanomas and correlate them with some histological parameters. ANIMALS AND METHODS: Forty-eight samples (38 pigmented and 10 non-pigmented melanomas) were first selected and their nature confirmed using S100, Melan A and vimentin. All cases were examined by immunohistochemistry using S100A4 to correlate expression, histotype, and level of invasion. RESULTS: All the tumors, including 10 non-pigmented, were positive for S100, Melan A, vimentin and negative for cytokeratin AE1/AE3 (consistent with melanomas). The 48 melanomas were classified as epithelioid (n = 21), spindle (n = 14), and mixed (n = 13). S100A4 was preferentially expressed in epithelioid and spindle cell types compared with mixed melanomas and S100A4 expression was not associated with level of invasion (Clark's levels IV to V). CONCLUSION: S100A4 expression in melanoma samples varied among histotypes but not between levels of invasion.
Assuntos
Doenças do Cão/patologia , Melanoma/veterinária , Neoplasias Cutâneas/veterinária , Animais , Brasil , Doenças do Cão/classificação , Cães , Imuno-Histoquímica/veterinária , Antígeno MART-1/biossíntese , Melanoma/classificação , Melanoma/patologia , Estadiamento de Neoplasias , Neoplasias Cutâneas/classificação , Neoplasias Cutâneas/patologia , Células Estromais/patologia , Vimentina/biossínteseRESUMO
BACKGROUND: A captive adult male saddleback tamarin, Saguinus fuscicollis, was lethargic, unresponsive to its surrounding and died before medical care. METHODS: Necroscopic, histopathological and immunohistochemical examinations were performed. RESULTS: Neoplastic spindle cells of the urinary bladder were positive for antifactor VIII antibody. CONCLUSION: A primary urinary bladder haemangiosarcoma was diagnosed, and it has not been previously reported in non-human primates.
Assuntos
Hemangiossarcoma/veterinária , Doenças dos Macacos , Saguinus , Neoplasias da Bexiga Urinária/veterinária , Animais , Evolução Fatal , Hemangiossarcoma/patologia , Masculino , Bexiga Urinária/patologia , Neoplasias da Bexiga Urinária/patologiaRESUMO
BACKGROUND: Due to numerous complications associated to gastrointestinal augmented cystoplasty, this study aimed to analyze the anatomic repair of the bladder of 10 female dogs using grafts of porcine small intestinal submucosa (SIS) seeded with cultured homologous smooth muscle cells, and compare them with the acellular SIS grafts. RESULTS: We assessed the possible side effects and complications of each type of graft by clinical examination, abdominal ultrasound and laboratory findings. Anatomic repair of neoformed bladder was assessed by histological staining for H/E and Masson's Trichrome, analyzed with a Nikon Photomicroscope connected to the system of image analysis Image J. CONCLUSIONS: We propose that SIS associated to homologous smooth cells can improve the quality of tissue repair, and consequently decrease the potential complications inherent to acellular SIS.
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Transplante de Células/veterinária , Cães , Intestino Delgado , Miócitos de Músculo Liso , Bexiga Urinária/patologia , Animais , Transplante de Células/métodos , Técnicas de Cocultura , Colágeno/fisiologia , Feminino , SuínosRESUMO
BACKGROUND: Reports of neoplasms in Panthera species are increasing, but they are still an uncommon cause of disease and death in captive wild felids. The presence of two or more primary tumor in large felids is rarely reported, and there are no documented cases of ocular melanoma and mammary mucinous carcinoma in African lions. CASE PRESENTATION: An ocular melanoma and a mammary mucinous carcinoma are described in an African lion (Panthera leo). The first tumour was histologically characterized by the presence of epithelioid and fusiform melanocytes, while the latter was composed of mucus-producing cells with an epithelial phenotype that contained periodic acid-Schiff (PAS) and Alcian blue staining mucins. Metastases of both tumor were identified in various organs and indirect immunohistochemistry was used to characterize them. Peribiliary cysts were observed in the liver. CONCLUSIONS: This is the first description of these tumor in African lions.
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Adenocarcinoma Mucinoso/veterinária , Animais de Zoológico , Neoplasias Oculares/veterinária , Leões , Neoplasias Mamárias Animais/patologia , Melanoma/veterinária , Adenocarcinoma Mucinoso/patologia , Animais , Neoplasias Oculares/patologia , Neoplasias Oculares/cirurgia , Feminino , Neoplasias Hepáticas/secundário , Neoplasias Hepáticas/veterinária , Melanoma/patologia , Melanoma/cirurgiaRESUMO
A 6-year-old Girolando dairy cow was presented for evaluation of a large subcutaneous facial mass. Fine-needle aspirates of the mass contained many neoplastic cells with high nuclear:cytoplasmic ratios arranged in sheets and loosely cohesive clusters with streaming erythrocytes and neutrophils in the background. Neoplastic cells were 13-25 µm in diameter and were round to cuboidal with variably distinct borders. Based on the signalment, anatomic location, and cytologic findings, differential diagnoses included salivary adenocarcinoma, squamous cell carcinoma, and mucoepidermoid carcinoma. The cow was euthanized and a necropsy was performed. The primary neoplasm arose from the left parotid salivary gland and meastatic tumor was found in the regional lymph nodes and lung. Histologically, the tumor was composed of anastomosing and irregular solid islets surrounded by scant stroma. Cells were negative for periodic acid-Schiff (PAS), PAS-diastase, and Alcian blue pH 2.5 stains, used to detect mucin. On immunohistochemical analysis, neoplastic luminal salivary gland cells expressed cytokeratin, but not S100, α-smooth muscle actin, or vimentin. Peripheral cells of neoplastic islets were immunoreactive for p63. The final diagnosis was nonsecretory adenocarcinoma of the parotid salivary gland.
Assuntos
Adenocarcinoma/veterinária , Doenças dos Bovinos/patologia , Neoplasias Pulmonares/veterinária , Glândula Parótida/patologia , Neoplasias Parotídeas/veterinária , Adenocarcinoma/patologia , Animais , Biópsia por Agulha Fina , Bovinos , Diagnóstico Diferencial , Face/patologia , Evolução Fatal , Feminino , Queratinas/metabolismo , Pulmão/patologia , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/secundário , Linfonodos/patologia , Metástase Linfática , Neoplasias Parotídeas/patologiaRESUMO
BACKGROUND: Spontaneous gastrointestinal neoplasms in non-human primates are commonly seen in aged individuals. Due to genetic similarities between human and non-human primates, scientists have shown increasing interest in terms of comparative oncology studies. CASE PRESENTATION: The present study is related to a case of an intestinal leiomyoma in a black crested macaque (Macaca nigra), kept on captivity by Matecaña Zoo, Pereira City, Colombia. The animal had abdominal distension, anorexia, vomiting, diarrhea and behavioral changes. Clinical examination showed an increased volume in the upper right abdominal quadrant caused by a neoplastic mass. The patient died during the surgical procedure. Necropsy revealed several small nodules in the peritoneum with adhesion to different portions of the small and large intestines, liver, stomach and diaphragm. Tissue samples were collected, routinely processed and stained by H&E. Microscopic examination revealed a mesenchymal tumor limited to tunica muscularis, resembling normal smooth muscle cells. Neoplastic cells were positive for alpha-smooth muscle actin and vimentin, and negative for cytokeratin AE1/AE3 by immunohistochemistry. Those morphological and immunohistochemical findings allowed to diagnose the intestinal leiomyoma referred above. CONCLUSION: Neoplastic diseases in primates have multifaceted causes. Their manifestations are understudied, leading to a greater difficulty in detection and measurement of the real impact provides by this disease.
Assuntos
Neoplasias Intestinais/veterinária , Intestino Delgado/patologia , Leiomioma/veterinária , Macaca , Doenças dos Macacos/patologia , Animais , Feminino , Neoplasias Intestinais/patologia , Leiomioma/patologiaRESUMO
The present study aimed to describe a simple technique for enema administration in one-day-old broiler chicks. For this purpose we used 455 unsexed health birds divided into four groups submitted to three different experimental protocols: in the first one, we measured the total length of the large intestine in order to establish a secure distance for probe introduction; in the second, we evaluated maximum compliance of large intestine and diffusion range; finally, based on results obtained we tested the hypothesis in 400 birds in order to standardize the method. Enema solutions applied in an intrarectal manner with a stainless steel gavage BD-10 probe into one-day-old broiler chicks at 0.2 mL at a distance of 1.5 cm proved to be a reliable method.
Assuntos
Enema/métodos , Animais , Galinhas , Azul de MetilenoAssuntos
Alouatta/parasitologia , Doenças dos Macacos/patologia , Toxoplasma/imunologia , Toxoplasmose Animal/patologia , Animais , Biópsia por Agulha Fina/veterinária , Diagnóstico Diferencial , Evolução Fatal , Feminino , Hepatomegalia/veterinária , Fígado/parasitologia , Fígado/patologia , Doenças dos Macacos/parasitologia , Baço/parasitologia , Baço/patologia , Esplenomegalia/veterinária , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/parasitologiaRESUMO
BACKGROUND: Salivary mucocele is an accumulation of saliva in a single or multiloculated cavity lined by connective tissue that is contiguous to a salivary gland-duct complex and is the most common condition affecting the salivary glands in dogs. Occasionally, different types of metaplastic lesions, such as squamous and osseous metaplasia - which are rare lesions in animals - can be observed in association with salivary mucocele. CASE PRESENTATION: A right facial enlargement was suddenly observed in a 4-year-old non-spayed female Shih-Tzu dog. The lesion presented itself as a soft and fluctuant mass located in the right side of the face near to the neck. Histologically, the mass consisted of a cavitary formation without an epithelial lining. Additionally, microscopic examination revealed the presence of osteoid-producing cells which gave rise to areas of bone formation, probably induced by irritation due to the presence sialoliths. Such cells and bone formations were also present in the cavity wall, consequently leading us to classify the condition as a salivary mucocele with osseous metaplasia. CONCLUSIONS: In the present case, the pathogenesis was probably associated with the presence of sialoliths, which can behave as etiological agents for the metaplastic lesion. The occurrence of osteoid metaplasia is a rare peculiar condition in the canine salivar y gland, and due to the rarity and lack of information about this specific disease, no clinical data can yet be associated with the development of salivary mucocele with osseous metaplasia in dogs.