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Phenylalanine ammonia-lyase (PAL) is an essential enzyme in the phenylpropanoid pathway, in which numerous aromatic intermediate metabolites play significant roles in plant growth, adaptation, and disease resistance. Cultivated peanuts are highly susceptible to Aspergillus flavus L. infection. Although PAL genes have been characterized in various major crops, no systematic studies have been conducted in cultivated peanuts, especially in response to A. flavus infection. In the present study, a systematic genome-wide analysis was conducted to identify PAL genes in the Arachis hypogaea L. genome. Ten AhPAL genes were distributed unevenly on nine A. hypogaea chromosomes. Based on phylogenetic analysis, the AhPAL proteins were classified into three groups. Structural and conserved motif analysis of PAL genes in A. hypogaea revealed that all peanut PAL genes contained one intron and ten motifs in the conserved domains. Furthermore, synteny analysis indicated that the ten AhPAL genes could be categorized into five pairs and that each AhPAL gene had a homologous gene in the wild-type peanut. Cis-element analysis revealed that the promoter region of the AhPAL gene family was rich in stress- and hormone-related elements. Expression analysis indicated that genes from Group I (AhPAL1 and AhPAL2), which had large number of ABRE, WUN, and ARE elements in the promoter, played a strong role in response to A. flavus stress.
Assuntos
Arachis , Aspergillus flavus , Aspergillus flavus/genética , Arachis/genética , Arachis/metabolismo , Fenilalanina Amônia-Liase/genética , Fenilalanina Amônia-Liase/metabolismo , Filogenia , Regiões Promotoras GenéticasRESUMO
Preparing a massive mechanical resonator in a state with quantum limited motional energy provides a promising platform for studying fundamental physics with macroscopic systems and allows to realize a variety of applications, including precise sensing. While several demonstrations of such ground-state cooled systems have been achieved, in particular in sideband-resolved cavity optomechanics, for many systems overcoming the heating from the thermal bath remains a major challenge. In contrast, optomechanical systems in the sideband-unresolved limit are much easier to realize due to the relaxed requirements on their optical properties, and the possibility to use a feedback control schemes to reduce the motional energy. The achievable thermal occupation is ultimately limited by the correlation between the measurement precision and the back-action from the measurement. Here, we demonstrate measurement-based feedback cooling on a fully integrated optomechanical device fabricated using a pick-and-place method, operating in the deep sideband-unresolved limit. With the large optomechanical interaction and a low thermal decoherence rate, we achieve a minimal average phonon occupation of 0.76 when pre-cooled with liquid helium and 3.5 with liquid nitrogen. Significant sideband asymmetry for both bath temperatures verifies the quantum character of the mechanical motion. Our method and device are ideally suited for sensing applications directly operating at the quantum limit, greatly simplifying the operation of an optomechanical system in this regime.
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The rapid development of high-QM macroscopic mechanical resonators has enabled great advances in optomechanics. Further improvements could allow for quantum-limited or quantum-enhanced applications at ambient temperature. Some of the remaining challenges include the integration of high-QM structures on a chip, while simultaneously achieving large coupling strengths through an optical read-out. Here, we present a versatile fabrication method, which allows us to build fully integrated optomechanical structures. We place a photonic crystal cavity directly above a mechanical resonator with high-QM fundamental out-of-plane mode, separated by a small gap. The highly confined optical field has a large overlap with the mechanical mode, enabling strong optomechanical interaction strengths. Furthermore, we implement a novel photonic crystal design, which allows for a very large cavity photon number, a highly important feature for optomechanical experiments and sensor applications. Our versatile approach is not limited to our particular design but allows for integrating an out-of-plane optical read-out into almost any device layout. Additionally, it can be scaled to large arrays and paves the way to realizing quantum experiments and applications with mechanical resonators based on high-QM out-of-plane modes alike.
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Preparing mechanical systems in their lowest possible entropy state, the quantum ground state, starting from a room temperature environment is a key challenge in quantum optomechanics. This would not only enable creating quantum states of truly macroscopic systems, but at the same time also lay the groundwork for a new generation of quantum-limited mechanical sensors in ambient environments. Laser cooling of optomechanical devices using the radiation pressure force combined with cryogenic precooling has been successful at demonstrating ground state preparation of various devices, while a similar demonstration starting from a room temperature environment remains an outstanding goal. Here, we combine integrated nanophotonics with phononic band gap engineering to simultaneously overcome prior limitations in the isolation from the surrounding environment and the achievable mechanical frequencies, as well as limited optomechanical coupling strength, demonstrating a single-photon cooperativity of 200. This new microchip technology allows us to feedback cool a mechanical resonator to around 1 mK, near its motional ground state, from room temperature. Our experiment marks a major step toward accessible, widespread quantum technologies with mechanical resonators.
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Low-dimensional narrow band gap III-V compound semiconductors, such as InAs and InSb, have attracted much attention as one of promising platforms for studying Majorana zero modes and non-Abelian statistics relevant for topological quantum computation. So far, most of experimental studies were performed on hybrid devices based on one-dimensional semiconductor nanowires. In order to build complex topological circuits toward scalable quantum computing, exploring high-mobility two-dimensional (2D) III-V compound electron system with strong spin-orbit coupling is highly desirable. Here, we study quantum transport in high-mobility InSb nanosheet grown by molecular-beam epitaxy. The observations of Shubnikov-de Hass oscillations and quantum Hall states, together with the angular dependence of magnetotransport measurements, provide the evidence for the 2D nature of electronic states in InSb nanosheet. The presence of strong spin-orbit coupling in the InSb nanosheet is verified by the low-field magnetotransport measurements, characterized by weak antilocalization effect. Finally, we demonstrate the realization of high-quality InSb nanosheet-superconductor junctions with transparent interface. Our results not only advance the study of 2D quantum transport but also open up opportunities for developing hybrid topological devices based on 2D semiconducting nanosheets with strong spin-orbit coupling.
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Demand for lightweight, highly reflective and mechanically compliant mirrors for optics experiments has seen a significant surge. In this aspect, photonic crystal (PhC) membranes are ideal alternatives to conventional mirrors, as they provide high reflectivity with only a single suspended layer of patterned dielectric material. However, due to limitations in nanofabrication, these devices are usually not wider than 300 µm. Here we experimentally demonstrate suspended PhC mirrors spanning areas up to 10 × 10 mm2. We overcome limitations imposed by the size of the PhC and measure reflectivities greater than 90 % on 56 nm thick mirrors at a wavelength of 1550 nm-an unrivaled performance compared to PhC mirrors with micro scale diameters. These structures bridge the gap between nano scale technologies and macroscopic optical elements.
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Mechanical oscillators are at the heart of many sensor applications. Recently several groups have developed oscillators that are probed optically, fabricated from high-stress silicon nitride films. They exhibit outstanding force sensitivities of a few aN/Hz1/2 and can also be made highly reflective, for efficient detection. The optical read-out usually requires complex experimental setups, including positioning stages and bulky cavities, making them impractical for real applications. In this paper we propose a novel way of building fully integrated all-optical force sensors based on low-loss silicon nitride mechanical resonators with a photonic crystal reflector. We can circumvent previous limitations in stability and complexity by simulating a suspended focusing photonic crystal, purely made of silicon nitride. Our design allows for an all integrated sensor, built out of a single block that integrates a full Fabry-Pérot cavity, without the need for assembly or alignment. The presented simulations will allow for a radical simplification of sensors based on high-Q silicon nitride membranes. Our results comprise, to the best of our knowledge, the first simulations of a focusing mirror made from a mechanically suspended flat membrane with subwavelength thickness. Cavity lengths between a few hundred µm and mm should be directly realizable.
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A variety of micro-tweezers techniques, such as optical tweezers, magnetic tweezers, and dielectrophoresis technique, have been applied intensively in precise characterization of micro/nanoparticles and bio-molecules. They have contributed remarkably in better understanding of working mechanisms of individual sub-cell organelles, proteins, and DNA. In this paper, we present a controllable electrostatic device embedded in a microchannel, which is capable of driving, trapping, and releasing charged micro-particles suspended in microfluid, demonstrating the basic concepts of electrostatic tweezers. Such a device is scalable to smaller size and offers an alternative to currently used micro-tweezers for application in sorting, selecting, manipulating, and analyzing individual micro/nanoparticles. Furthermore, the system offers the potential in being combined with dielectrophoresis and other techniques to create hybrid micro-manipulation systems.
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Transition metal carbides (TMCs) are a large family of materials with many intriguing properties and applications, and high-quality 2D TMCs are essential for investigating new physics and properties in the 2D limit. However, the 2D TMCs obtained so far are chemically functionalized, defective nanosheets having maximum lateral dimensions of â¼10 µm. Here we report the fabrication of large-area high-quality 2D ultrathin α-Mo2C crystals by chemical vapour deposition (CVD). The crystals are a few nanometres thick, over 100 µm in size, and very stable under ambient conditions. They show 2D characteristics of superconducting transitions that are consistent with Berezinskii-Kosterlitz-Thouless behaviour and show strong anisotropy with magnetic field orientation; moreover, the superconductivity is also strongly dependent on the crystal thickness. Our versatile CVD process allows the fabrication of other high-quality 2D TMC crystals, such as ultrathin WC and TaC crystals, which further expand the large family of 2D materials.
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Tunable and mode-locked laser operation near 2 µm based on different Tm-doped YAG ceramics, 4 at.% and 10 at.%, is demonstrated. Several designs of GaSb-based surface-quantum-well SESAMs are characterized and studied as saturable absorbers for mode-locking. Best mode-locking performance was achieved using an antireflection-coated near-surface quantum-well SESAM, resulting in a pulse duration of ~3 ps and ~150 mW average output power at 89 MHz. All mode-locked Tm:YAG ceramic lasers operated at 2012 nm, with over 133 nm demonstrated tuning for continuous-wave operation.
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Multiwalled carbon nanotube (MWCNT)-fused silica composite powders were synthesized by solgel method and dense bulk composites were successfully fabricated via hot-pressing. This composite was characterized by XRD, HRTEM, and FESEM. MWCNTs in the hot-pressed composites are in their integrity observed by HRTEM. The electrical properties of MWCNT-fused silica composites were measured and analyzed. The electrical resistivity was found to decrease with the increase in the amount of the MWCNT loading in the composite. When the volume percentage of the MWCNTs increased to 5 vol%, the electrical resistivity of the composite is 24.99 omega cm, which is a decrease of twelve orders of value over that of pure fused silica matrix. The electrical resistivity further decreases to 1.742 omega. cm as the concentration of the MWCNTs increased to 10 vol%. The dielectric properties of the composites were also measured at the frequency ranging from 12.4 to 17.8 GHz (Ku band) at room temperature. The experimental results reveal that the dielectric properties are extremely sensitive to the volume percentage of the MWCNTs, and the permittivities, especially the imaginary permittivities, increase dramatically with the increase in the concentration of the MWCNTs. The improvement of dielectric properties in high frequency region mainly originates from the greatly increasing electrical properties of the composite.