RESUMO
KEY MESSAGE: Powdery mildew resistance gene MlWE74, originated from wild emmer wheat accession G-748-M, was mapped in an NBS-LRR gene cluster of chromosome 2BS. Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a globally devastating disease. Wild emmer wheat (Triticum turgidum var. dicoccoides) is a valuable genetic resource for improving disease resistance in common wheat. A powdery mildew resistance gene was transferred to hexaploid wheat line WE74 from wild emmer accession G-748-M. Genetic analysis revealed that the powdery mildew resistance in WE74 is controlled by a single dominant gene, herein temporarily designated MlWE74. Bulked segregant analysis (BSA) and molecular mapping delimited MlWE74 to the terminal region of chromosome 2BS flanking by markers WGGBD412 and WGGBH346 within a genetic interval of 0.25 cM and corresponding to 799.9 kb genomic region in the Zavitan reference sequence. Sequence annotation revealed two phosphoglycerate mutase-like genes, an alpha/beta-hydrolases gene, and five NBS-LRR disease resistance genes that could serve as candidates for map-based cloning of MlWE74. The geographical location analysis indicated that MlWE74 is mainly distributed in Rosh Pinna and Amirim regions, in the northern part of Israel, where environmental conditions are favorable to the occurrence of powdery mildew. Moreover, the co-segregated marker WGGBD425 is helpful in marker-assisted transfer of MlWE74 into elite cultivars.
Assuntos
Resistência à Doença , Triticum , Mapeamento Cromossômico , Cromossomos de Plantas , Resistência à Doença/genética , Genes de Plantas , Família Multigênica , Doenças das Plantas/genética , Triticum/genéticaRESUMO
Benzene is an occupational toxicant and an environmental pollutant that potentially causes hematotoxicity and leukemia in exposed populations. Epidemiological studies suggest an association between an increased incidence of childhood leukemia and benzene exposure during the early stages of pregnancy. However, experimental evidence supporting the association is lacking at the present time. It is believed that benzene and its metabolites target hematopoietic stem cells (HSCs) to cause toxicity and cancer in the hematopoietic system. In the current study, we compared the effects of hydroquinone (HQ), a major metabolite of benzene in humans and animals, on mouse embryonic yolk sac hematopoietic stem cells (YS-HSCs) and adult bone marrow hematopoietic stem cells (BM-HSCs). YS-HSCs and BM-HSCs were isolated and enriched, and were exposed to HQ at increasing concentrations. HQ reduced the proliferation and the differentiation and colony formation, but increased the apoptosis of both YS-HSCs and BM-HSCs. However, the cytotoxic and apoptotic effects of HQ were more apparent and reduction of colony formation by HQ was more severe in YS-HSCs than in BM-HSCs. Differences in gene expression profiles were observed in HQ-treated YS-HSCs and BM-HSCs. Cyp4f18 was induced by HQ both in YS-HSCs and BM-HSCs, whereas DNA-PKcs was induced in BM-HSCs only. The results revealed differential effects of benzene metabolites on embryonic and adult HSCs. The study established an experimental system for comparison of the hematopoietic toxicity and leukemogenicity of benzene and metabolites during mouse embryonic development and adulthood.
Assuntos
Células-Tronco Adultas/efeitos dos fármacos , Células-Tronco Hematopoéticas/efeitos dos fármacos , Hidroquinonas/toxicidade , Saco Vitelino/citologia , Células-Tronco Adultas/citologia , Animais , Apoptose/efeitos dos fármacos , Apoptose/genética , Benzeno/metabolismo , Benzeno/toxicidade , Células da Medula Óssea/citologia , Células da Medula Óssea/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Ensaio de Unidades Formadoras de Colônias , Embrião de Mamíferos/citologia , Células-Tronco Hematopoéticas/citologia , Masculino , Camundongos , Saco Vitelino/efeitos dos fármacosRESUMO
In vitro cell cultures are currently tested for their application as a biological tool for enhanced monitoring and field evaluation of environmental toxic chemical pollution. Here cell lines established from green sea turtles (GSTs) were comparatively tested for their cytotoxic responses to mercury chloride (HgCl2) exposure and also their potential use as a biological tool for effective monitoring and screening of mercury contamination in environmental waters. Following a 24-h exposure to different concentrations of mercury solution, marine turtle cells were evaluated for their cytotoxic responses using three different endpoint bioassays: tetrazolium salt reduction (MTT), neutral red uptake (NR), and Coomassie blue (CB) methods. Cytotoxic sensitivities of GST cell lines to HgCl2 were determined and compared basing on their 50% inhibition concentration (IC50) values calculated from these tests. These marine turtle cells share a very different pattern of cytotoxic sensitivities and reactions to inorganic Mercury. Among these nine turtle cell lines, turtle liver cells (GST-LV) appear to be the most tolerant one to mercury exposure while turtle lung cells (GST-LG) exhibit to be the most sensitive one. Results from this in vitro study correlate well with in vivo examination of mercury concentration in the tissues of marine turtles and are also validated and ascertained by calculated regression equations showing a significant correlation (P<0.01) between these test methods. This study also reveals the cytotoxic effect of inorganic mercury on in vitro green turtle cells and also shows GST-LG to be a cell line with potential application in field monitoring and assessing mercury contamination as a bioindicator.