RESUMO
This study analyzed the effects of brassinolide (BL) on Na⺠accumulation, leaf physiological characteristics and differentially expressed genes (DEGs) of cotton leaves under NaCl stress. The results showed that NaCl stress increased the Naâº, proline and MDA content in the leaves of Sumian 12 and Sumian 22, and changed the expression level of genes in cotton leaves. The application of BL counteracted the NaCl stress-induced growth inhibition in the two tested cotton cultivars. It reduced the accumulation of Naâº, enhanced proline content, and resulted in a decrease in the MDA content of NaCl-stressed leaves, and the influence of BL on salt-stressed Sumian 12 plants was more pronounced than that on Sumian 22. The digital gene expression analysis in Sumian 12 indicated that BL application significantly influenced the gene expression in NaCl-stressed cotton leaves, the gene expression pattern as a result of the root applied BL on NaCl-stressed cotton treatment (BL+NaCl) was similar to the normal cotton plants (CK). Our results indicated that brassinolide alleviated NaCl stress on cotton through improving leaf physiological characteristics and gene expression, and resulted in an increase in biomass of NaCl-stressed cotton.
Assuntos
Gossypium/fisiologia , Folhas de Planta/fisiologia , Cloreto de Sódio , Estresse Fisiológico , Transcriptoma , Biomassa , Brassinosteroides , Regulação da Expressão Gênica de Plantas , Malondialdeído/química , Prolina/química , Sódio/química , Esteroides HeterocíclicosRESUMO
The Cys2/His2-type zinc finger proteins have been implicated in different cellular processes involved in plant development and stress responses. Through microarray analysis, a salt-responsive zinc finger protein gene ZFP179 was identified and subsequently cloned from rice seedlings. ZFP179 encodes a 17.95 kDa protein with two C2H2-type zinc finger motifs having transcriptional activation activity. The real-time RT-PCR analysis showed that ZFP179 was highly expressed in immature spikes, and markedly induced in the seedlings by NaCl, PEG 6000, and ABA treatments. Overexpression of ZFP179 in rice increased salt tolerance and the transgenic seedlings showed hypersensitivity to exogenous ABA. The increased levels of free proline and soluble sugars were observed in transgenic plants compared to wild-type plants under salt stress. The ZFP179 transgenic rice exhibited significantly increased tolerance to oxidative stress, the reactive oxygen species (ROS)-scavenging ability, and expression levels of a number of stress-related genes, including OsDREB2A, OsP5CS OsProT, and OsLea3 under salt stress. Our studies suggest that ZFP179 plays a crucial role in the plant response to salt stress, and is useful in developing transgenic crops with enhanced tolerance to salt stress.
Assuntos
Oryza/metabolismo , Proteínas de Plantas/metabolismo , Tolerância ao Sal , Dedos de Zinco , Ácido Abscísico/farmacologia , Sequência de Aminoácidos , Metabolismo dos Carboidratos/efeitos dos fármacos , Clonagem Molecular , Sequestradores de Radicais Livres/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Peróxido de Hidrogênio/metabolismo , Dados de Sequência Molecular , Oryza/efeitos dos fármacos , Oryza/enzimologia , Oryza/genética , Estresse Oxidativo/efeitos dos fármacos , Proteínas de Plantas/química , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas , Prolina/metabolismo , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/genética , Tolerância ao Sal/efeitos dos fármacos , Tolerância ao Sal/genética , Plântula/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Alinhamento de Sequência , Análise de Sequência de Proteína , Solubilidade/efeitos dos fármacos , Estresse Fisiológico/efeitos dos fármacos , Estresse Fisiológico/genética , Transativadores/metabolismoRESUMO
We previously identified a salt and drought stress-responsive TFIIIA-type zinc finger protein gene ZFP252 from rice. Here we report the functional analysis of ZFP252 using gain- and loss-of-function strategies. We found that overexpression of ZFP252 in rice increased the amount of free proline and soluble sugars, elevated the expression of stress defense genes and enhanced rice tolerance to salt and drought stresses, as compared with ZFP252 antisense and non-transgenic plants. Our findings suggest that ZFP252 plays an important role in rice response to salt and drought stresses and is useful in engineering crop plants with enhanced tolerance to salt and drought stresses.
Assuntos
Oryza/genética , Proteínas de Plantas/metabolismo , Fator de Transcrição TFIIIA/metabolismo , Metabolismo dos Carboidratos , Desidratação , Expressão Gênica , Genes de Plantas , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas/efeitos dos fármacos , Plantas Geneticamente Modificadas/metabolismo , Prolina/metabolismo , Cloreto de Sódio/farmacologia , Fator de Transcrição TFIIIA/genéticaRESUMO
A rice zinc-finger protein gene, RZF71, encoding the C2H2-type zinc-finger transcription factor was isolated from rice (Oryza sativa L. subs. Japonica) by RT-PCR approach. Gene RZF71 encodes a 25 kDa protein with 250 amino acids, which contains two typical C2H2 zinc finger domains. The expression profiling showed that RZF71 was constitutively expressed in roots, culms, leaves, and flowering spikes. The semi-quantitative RT-PCR assay showed RZF71 was strongly induced by high-salinity and 20% PEG6000 treatments, but not regulated by low temperature and ABA (abscisic acid) treatments. Tran-sient expression of the RZF71-GFP protein in onion epidermal cell showed that RZF71 was localized in cell nuclei. These results indicated that the RZF71 may play an important role in rice responses to salt and osmotic stresses as a transcription factor.