Metasurface-Dressed Two-Dimensional on-Chip Waveguide for Free-Space Light Field Manipulation.

We show that a metasurface-coated two-dimensional (2D) slab waveguide enables the generation of arbitrary complex light fields by combining the extreme versatility and freedom on the wavefront control of optical metasurfaces with the compactness of photonic integrated circuits. We demonstrated off-chip 2D focusing and holographic projection with our metasurface-dressed photonic integrated devices. This technology holds the potential for many other optical applications requiring 2D light field manipulation with full on-chip integration, such as solid-state LiDAR and near-eye AR/VR displays.

Presence of Tertiary Lymphoid Organ in Nasal Inverted Papilloma Is Correlated with Eosinophil Infiltration and Local Immunoglobulin Production.

INTRODUCTION: Nasal inverted papilloma (NIP) is a benign tumour with multiple inflammatory cell infiltration. Tertiary lymphoid organs (TLOs) support local antibody production and play important roles in airway inflammation. However, the evidence of TLOs and local immunoglobulins in NIP has not been reported yet. We investigated the presence of TLOs and immunoglobulins in NIP tissues and their association with the clinical-pathological characteristics of NIPs. METHODS: We analyzed the occurrence and composition of TLOs and local immunoglobulins by immunohistochemistry and evaluated the lymph organogenesis associated genes and cytokines by quantitative qPCR and Luminex assays, respectively, in papilloma tissues from 84 NIP cases. RESULTS: TLOs were present in 54% (45/84) of the NIP patients but not in control subjects. TLOs were composed of T cells, B cells, follicular dendritic cells, macrophages, and natural killer cells. Compared to NIP tissues without TLOs, tissues with TLOs showed significantly higher eosinophil infiltration levels (3.5-fold), elevation of lymphorganogenic genes (CXCL12, CXCL13, CCL20, CCL21, CD21L, and lymphotoxin alpha and beta), and increased Th17 (IL-21, IL-22, and GM-CSF) and Th2 (IL-5 and IL-13) cytokine production. Moreover, NIP with TLOs demonstrated a higher number of follicular T helper cells and immunoglobulin-producing plasma cells (CD138+ IgA+, CD138+ IgM+, CD138+ IgE+, and CD138+ IgG+) than those without TLOs, and these antibody-producing cells were positively correlated with the eosinophil number. CONCLUSION: The high frequency of TLOs and excess local immunoglobulin production are associated with an eosinophilic and Th2 skew microenvironment in the NIP mucosa, which would contribute to an important immunopathogenic response during NIP pathogenesis.

Local IL-17 positive T cells are functionally associated with neutrophil infiltration and their development is regulated by mucosal microenvironment in nasal polyps.

OBJECTIVE AND DESIGN: IL-17 plays essential roles in neutrophilic inflammation in the lower respiratory tract, however, the characteristics of local IL-17+ T cells in nasal inflammatory mucosa are not fully understood. We investigated the roles of IL-17+ T cells in regulating neutrophil infiltration and the effect of the mucosal microenvironment in modulating IL-17+ T cell differentiation in CRSwNP tissues. SUBJECTS: 47 polyp tissues from chronic rhinosinusitis with nasal polyps (CRSwNP) patients without corticosteroid therapy and 26 tissues from healthy mucosa were obtained. METHODS: Immunohistochemistry and flow cytometry were used to analyze the neutrophil infiltration, local IL-17+ T cell subsets, as well as cytokine producing profiles of IL-17+ T cell; tissue homogenates were used to study neutrophil migration and IL-17+ T cell differentiation. RESULTS: Increase of IL-17+ cells and IL-17+ T cell subsets was significant in polyp tissues versus controls, IL-17+ cell number was positively correlated with neutrophil infiltration; while homogenates from polyp tissues with high IL-17 promoted neutrophil migration in vitro. IL-17 response was found in polyp-derived T cells upon Staphylococcus aureus infection. IL-17+ T cells were also down-regulated in polyps from patients treated with glucocorticoid steroids, and exhibited poly-functionality patterns in polyp tissues. Finally, IL-17+ T cell differentiation could be induced by IL-23, and homogenates from polyps could enhance IL-17+ T cell development. CONCLUSIONS: This study determined a functional association of IL-17+ T cells with neutrophils in CRSwNP, and revealed that polyp microenvironment could promote IL-17+ T cell differentiation, suggesting a potential feedback role for IL-17+ T cell development and local neutrophilic inflammation.

Molding free-space light with guided wave-driven metasurfaces.

Metasurfaces with unparalleled controllability of light have shown great potential to revolutionize conventional optics. However, they mainly require external light excitation, which makes it difficult to fully integrate them on-chip. On the other hand, integrated photonics enables packing optical components densely on a chip, but it has limited free-space light controllability. Here, by dressing metasurfaces onto waveguides, we molded guided waves into any desired free-space modes to achieve complex free-space functions, such as out-of-plane beam deflection and focusing. This metasurface also breaks the degeneracy of clockwise- and counterclockwise-propagating whispering gallery modes in an active microring resonator, leading to on-chip direct orbital angular momentum lasing. Our study shows a viable route toward complete control of light across integrated photonics and free-space platforms and paves a way for creating multifunctional photonic integrated devices with agile access to free space, which enables a plethora of applications in communications, remote sensing, displays, etc.

Nonlinear Chiral Meta-Mirrors: Enabling Technology for Ultrafast Switching of Light Polarization.

Photonic nanostructures that realize ultrafast switching of light polarization are essential to advancements in the area of optical information processing. The unprecedented flexibility of metasurfaces in light manipulation makes them a promising candidate for active polarization control. However, due to the lack of optical materials exhibiting a fast as well as large refractive index change, photonic metadevices capable of ultrafast polarization switching remain elusive. Here, an ultrathin nonlinear chiral meta-mirror consisting of an array of amorphous silicon (α-Si) split-ring resonators on top of a silver backplane is demonstrated as a feasible platform for picosecond all-optical polarization switching of near-infrared light at picojoule-per-resonator pump energies. This success was made possible by the high-quality-factor resonances of the proposed meta-atoms that enable the mirror to exhibit strong chiro- and enantioselectivity. Experimental results confirm that our meta-mirrors can be used to facilitate high-speed and power-efficient polarization-state modulators.

The N-terminal of NBPF15 causes multiple types of aggregates and mediates phase transition.

The neuroblastoma breakpoint family (NBPF) consists of 24 members that play an important role in neuroblastoma and other cancers. NBPF is an evolutionarily recent gene family that encodes several repeats of Olduvai domain and an abundant N-terminal region. The function and biochemical properties of both Olduvai domain and the N-terminal region remain enigmatic. Human NBPF15 encodes a 670 AA protein consisting of six clades of Olduvai domains. In this study, we synthesized and expressed full-length NBPF15, and purified a range of NBPF15 truncations which were analyzed using dynamic light scattering (DLS), superdex200 (S200), small-angle X-ray scattering (SAXS), far-UV circular dichroism (CD) spectroscopy, transmission electron microscope (TEM), and crystallography. We found that proteins containing both the N-terminal region and Olduvai domain are heterogeneous with multiple types of aggregates, and some of them underwent a liquid-to-solid phase transition, probably because of the entanglement within the N-terminal coiled-coil. Proteins that contain only the Olduvai domain are homogeneous extended monomers, and those with the conserved clade 1 (CON1) have manifested a tendency to crystallize. We suggest that the entanglements between the mosaic disorder-ordered segments in NBPF15 N terminus have triggered the multiple types of aggregates and phase transition of NBPF15 proteins, which could be associated with Olduvai-related cognitive dysfunction diseases.

Nonreciprocal metasurface with space-time phase modulation.

Creating materials with time-variant properties is critical for breaking reciprocity that imposes fundamental limitations on wave propagation. However, it is challenging to realize efficient and ultrafast temporal modulation in a photonic system. Here, leveraging both spatial and temporal phase manipulation offered by an ultrathin nonlinear metasurface, we experimentally demonstrated nonreciprocal light reflection at wavelengths around 860 nm. The metasurface, with travelling-wave modulation upon nonlinear Kerr building blocks, creates spatial phase gradient and multi-terahertz temporal phase wobbling, which leads to unidirectional photonic transitions in both the momentum and energy spaces. We observed completely asymmetric reflections in forward and backward light propagations over a large bandwidth around 5.77 THz within a sub-wavelength interaction length of 150 nm. Our approach highlights a potential means for creating miniaturized and integratable nonreciprocal optical components.

Characterization of protein expression levels with label-free detected reverse phase protein arrays.

In reverse-phase protein arrays (RPPA), one immobilizes complex samples (e.g., cellular lysate, tissue lysate or serum etc.) on solid supports and performs parallel reactions of antibodies with immobilized protein targets from the complex samples. In this work, we describe a label-free detection of RPPA that enables quantification of RPPA data and thus facilitates comparison of studies performed on different samples and on different solid supports. We applied this detection platform to characterization of phosphoserine aminotransferase (PSAT) expression levels in Acanthamoeba lysates treated with artemether and the results were confirmed by Western blot studies.