Simple Sol-Gel Protein Stabilization toward Rainbow and White Lighting Devices.

Fluorescent proteins (FPs) are heralded as a paradigm of sustainable materials for photonics/optoelectronics. However, their stabilization under non-physiological environments and/or harsh operation conditions is the major challenge. Among the FP-stabilization methods, classical sol-gel is the most effective, but less versatile, as most of the proteins/enzymes are easily degraded due to the need of multi-step processes, surfactants, and mixed water/organic solvents in extreme pH. Herein, sol-gel chemistry with archetypal FPs (mGreenLantern; mCherry) is revisited, simplifying the method by one-pot, surfactant-free, and aqueous media (phosphate buffer saline pH = 7.4). The synthesis mechanism involves the direct reaction of the carboxylic groups at the FP surface with the silica precursor, generating a positively charged FP intermediate that acts as a seed for the formation of size-controlled mesoporous FP@SiO2 nanoparticles. Green-/red-emissive (single-FP component) and dual-emissive (multi-FPs component; kinetic studies not required) FP@SiO2 are prepared without affecting the FP photoluminescence and stabilities (>6 months) under dry storage and organic solvent suspensions. Finally, FP@SiO2 color filters are applied to rainbow and white bio-hybrid light-emitting diodes featuring up to 15-fold enhanced stabilities without reducing luminous efficacy compared to references with native FPs. Overall, an easy, versatile, and effective FP-stabilization method is demonstrated in FP@SiO2 toward sustainable protein lighting.

Genetically Encoded Oligomerization for Protein-Based Lighting Devices.

Implementing proteins in optoelectronics represents a fresh idea toward a sustainable new class of materials with bio-functions that can replace environmentally unfriendly and/or toxic components without losing device performance. However, their native activity (fluorescence, catalysis, and so on) is easily lost under device fabrication/operation as non-native environments (organic solvents, organic/inorganic interfaces, and so on) and severe stress (temperature, irradiation, and so on) are involved. Herein, a gift bow genetically-encoded macro-oligomerization strategy is showcased to promote protein-protein solid interaction enabling i) high versatility with arbitrary proteins, ii) straightforward electrostatic driven control of the macro-oligomer size by ionic strength, and iii) stabilities over months in pure organic solvents and stress scenarios, allowing to integrate them into classical water-free polymer-based materials/components for optoelectronics. Indeed, rainbow-/white-emitting protein-based light-emitting diodes are fabricated, attesting a first-class performance compared to those with their respective native proteins: significantly enhanced device stabilities from a few minutes up to 100 h keeping device efficiency at high power driving conditions. Thus, the oligomerization concept is a solid bridge between biological systems and materials/components to meet expectations in bio-optoelectronics, in general, and lighting schemes, in particular.