RESUMO
Human fibroblasts with mutated type I collagen have marked defective adhesive capacities on exogenous type I collagen and exogenous fibronectin in comparison to normal fibroblasts. This defective cell adhesion could be partly explained by the decreased level of cell surface receptors of the beta 1-integrin family, i.e., the alpha 2 integrin subunit for type I collagen and the alpha 5 integrin subunit for fibronectin, observed in pathological fibroblasts. However, it appeared that the presence of altered collagen interfered both with fibronectin biosynthesis and with its surface expression. Using a binding assay on immobilized fibronectin, we demonstrated that the mutated collagen had a weaker binding to fibronectin. In addition, the pathological fibroblasts plated on a mixture of normal exogenous type I collagen and fibronectin exhibited the same maximal level of adhesion as control fibroblasts. These results indicate that fibroblasts with the mutated collagen exhibit a decreased binding to normal fibronectin, a modification of synthesis and surface expression of fibronectin, and, finally, altered adhesive capacities.
Assuntos
Colágeno/fisiologia , Fibroblastos/patologia , Fibronectinas/biossíntese , Adesão Celular/fisiologia , Colágeno/genética , Feminino , Feto , Fibronectinas/fisiologia , Humanos , MutaçãoRESUMO
The circulating forms of malignant cells from patients with Sezary syndrome exhibit on their glycoproteins a high level of beta (1-6)GlcNAc-branched N-linked oligosaccharides, a particular species of glycans related to the metastatic potential of several tumors and T lymphocytes activation. An increased activity of the N-acetylglucosaminyltransferase V and of the beta (1-4)galactosyltransferase, two enzymes implicated in beta (1-6)GlcNAc-branching is also found. Nevertheless, contrary to activated normal T lymphocytes, Sezary lymphocytes in agreement with their non-proliferating state, do not exhibit increased thymidine uptake. This result suggests that expression of the beta (1-6)GlcNAc-branched N-linked carbohydrates could be related to some of the malignant properties of Sezary lymphocytes.
Assuntos
Acetilglucosamina/sangue , Glicoproteínas/sangue , Linfócitos/metabolismo , Oligossacarídeos/sangue , Síndrome de Sézary/sangue , Neoplasias Cutâneas/sangue , Feminino , Glicoproteínas/química , Humanos , Ativação Linfocitária , Linfócitos/patologia , Masculino , Pessoa de Meia-Idade , N-Acetilglucosaminiltransferases/metabolismo , Síndrome de Sézary/enzimologia , Síndrome de Sézary/imunologia , Neoplasias Cutâneas/enzimologia , Neoplasias Cutâneas/imunologia , Linfócitos T/imunologia , beta-N-Acetilglucosaminilglicopeptídeo beta-1,4-Galactosiltransferase/metabolismoRESUMO
We have compared the mechanisms of ricin binding to and entry into Zajdela hepatoma cells (ZHC) and normal rat hepatocytes (HyC). Lactose but not mannan was found to inhibit ricin binding to and toxicity on ZHC and HyC. This finding suggests that ricin binding, entry, and toxicity are expressed only through the galactose binding sites on ZHC and HyC. Nevertheless, the characteristics of ricin binding and its entry pathway appeared to be different in several respects in ZHC and HyC. Scatchard analysis of equilibrium data determined over a wide range of 125I-labeled ricin concentrations yielded a curvilinear plot for ZHC, while a straight line was obtained for HyC. These results indicate that only ZHC possess high-affinity receptors for ricin. Analysis of ricin toxicity on ZHC and HyC, in the presence of ammonium chloride or after K+-depletion in both cell types, suggests that the ricin bound to galactose receptors entered through neutral vesicles in ZHC, and through both neutral and acidic vesicles in HyC. The qualitative and quantitative differences found between the process of receptor-mediated endocytosis of ricin in ZHC and HyC might explain the differential sensitivity of the two cell types toward the toxin.
Assuntos
Endocitose/efeitos dos fármacos , Lectinas Tipo C , Neoplasias Hepáticas Experimentais/metabolismo , Fígado/efeitos dos fármacos , Lectinas de Ligação a Manose , Ricina/toxicidade , Cloreto de Amônio , Animais , Linhagem Celular , Fígado/metabolismo , Masculino , Receptor de Manose , Potássio/metabolismo , Inibidores da Síntese de Proteínas/metabolismo , Inibidores da Síntese de Proteínas/toxicidade , Ratos , Ratos Endogâmicos , Receptores de Superfície Celular/efeitos dos fármacos , Receptores Imunológicos/efeitos dos fármacos , Ricina/metabolismoRESUMO
The binding to and toxicity of ricin on Zajdela hepatoma ascites cells were studied. The kinetic analysis of [125I]-ricin binding to hepatoma cells indicated that maximal specific binding was reached within 30 min. at 4 degrees C and 60 min. at 25 degrees C and that toxin binding to hepatoma cells was saturable. When the binding data were plotted according to the method of Scatchard, curvilinear graphs were obtained suggesting that hepatoma cells have both high and low affinity receptors for ricin. The number of high and low affinity receptors was identical at 4 and 25 degrees C, i.e., 8 x 10(5) and 1.2 x 10(7) sites per cell respectively. However, the capacity of hepatoma cells to bind ricin is stronger at 4 degrees C than at 25 degrees C. The toxic activity of ricin was totally abolished in the presence of lactose suggesting that ricin binding to cells occurs through binding sites containing galactosyl residues.