1.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 67(Pt 7): 827-9, 2011 Jul 01.
Artigo
em Inglês
| MEDLINE
| ID: mdl-21795805
RESUMO
Membrane-bound respiratory [NiFe] hydrogenase is an H2-uptake enzyme found in the periplasmic space of bacteria that plays a crucial role in energy-conservation processes. The heterodimeric unit of the enzyme from Hydrogenovibrio marinus was purified to homogeneity using chromatographic procedures. Crystals were grown using the sitting-drop vapour-diffusion method at room temperature. Preliminary crystallographic analysis revealed that the crystals belonged to space group P2(1), with unit-cell parameters a=75.72, b=116.59, c=113.40â Å, ß=91.3°, indicating that two heterodimers were present in the asymmetric unit.