Contrasting transport and fate of hydrophilic and hydrophobic bacteria in wettable and water-repellent porous media: Straining or attachment?

Bacterial transport and retention likely depend on bacterial and soil surface properties, especially hydrophobicity. We used a controlled experimental setup to explore hydrophilic Escherichia coli (E. coli) and hydrophobic Rhodococcus erythropolis (PTCC1767) (R. erythropolis) transport through dry (- 15,000 cm water potential) and water saturated (0 cm water potential) wettable and water-repellent sand columns. A pulse of bacteria (1 × 108 CFU mL-1) and bromide (10 mmol L-1) moved through the columns under saturated flow (0 cm) for four pore volumes. A second bacteria and bromide pulse was then poured on the column surfaces and leaching was extended six more pore volumes. In dry wettable sand attachment dominated E. coli retention, whereas R. erythropolis was dominated by straining. Once wetted, the dominant retention mechanisms flipped between these bacteria. Attachment by either bacteria decreased markedly in water-repellent sand, so straining was the main retention mechanism. We explain this from capillary potential energy, which enhanced straining under the formation of water films at very early times (i.e., imbibing) and film thinning at much later times (i.e., draining). The interaction between the hydrophobicity of bacteria and soil on transport, retention and release mechanisms needs greater consideration in predictions.

Transport, retention, and release of Escherichia coli and Rhodococcus erythropolis through dry natural soils as affected by water repellency.

Microbial transport in soil affects pathogen retention, colonization, and innoculant delivery in bioremediating agricultural soils. Various bacteria strains residing in the fluid phases of soils are potential contaminants affecting human health. We measured the transport of hydrophilic Escherichia coli (E. coli) and hydrophobic Rhodococcus erythropolis (R. erythropolis) bacteria through initially air-dried wettable or water-repellent soil columns to understand the effect of water repellency and the hydrophobicity of the organism on its retention, release, and transport properties. Bacteria suspensions infiltrated the top of the columns under saturated (0 cm) and unsaturated (-5 cm) flows in the air-dried (pulse 1) and rewetting (pulse 2) conditions. Cells were recovered from the leachates and the soil extracts by the viable counts. Wettable soil efficiently retained both hydrophobic and hydrophilic bacteria (>80%) in initial air-dried conditions (pulse 1). Even after rewetting, and the formation and expansion of water films and corresponding reduction of the air-water interfacial area (pulse 2), few bacteria were released (maximum 31.5% and 10.1% for saturated and unsaturated flows, respectively), whereas more cells were released from the water-repellent counterpart (more that 72%). The smaller size of hydrophobic R. erythropolis made cell transport possible within the thinner water films of both soils compared to hydrophilic E. coli through pulses 1 and 2. The shape of each strain's retention profiles was uniform and exponential as influenced by soil, strain, and water flow conditions. The results suggest that hydrophobic bacteria will disperse readily when leached into initially dry soil, while hydrophilic bacteria are more susceptible to leaching, posing a risk of pathogen contamination. Clearly the wettability of soil and organisms affects fate and transport.

Modeling Escherichia coli and Rhodococcus erythropolis transport through wettable and water repellent porous media.

Water protection and bioremediation strategies in the vadose zone require understanding the factors controlling bacterial transport for different hydraulic conditions. Breakthrough experiments were made in two different flow conditions: i) an initial bacteria pulse under ponded infiltration into dry sand (-15,000 cm); ii) a second bacteria pulse into the same columns during subsequent infiltration in constant water content and steady-state flow. Escherichia coli (E. coli) and Rhodococcus erythropolis (R. erythropolis) were used to represent hydrophilic and hydrophobic bacteria, respectively. Equilibrium and attachment/detachment models were tested to fit bromide (Br-) and bacteria transport data using HYDRUS-1D. Derjaguin-Landau-Verwey-Overbeek (DLVO) and extended DVLO (XDLVO) interaction energy profiles were calculated to predict bacteria sorption at particles. Adsorption of bacteria at air-water interfaces was estimated by a hydrophobic force approach. Results suggested greater retention of bacteria in water repellent sand compared with wettable sand. Inverse parameter optimization suggested that physico-chemical attachment of both E. coli and R. erythropolis was thousands of times lower in wettable than repellant sand and straining was 10-fold lower in E. coli for wettable vs repellant sand compared to the exact opposite by orders of magnitude with R. erythropolis. HYDRUS did not provide a clear priority of importance of solid-water or air-water interfaces in bacteria retention. Optimized model parameters did not show a clear relation to the (X)DLVO adsorption energies. This illustrated the ambivalence of (X)DLVO to predict bacterial attachment at solid soil particles of different wetting properties. Simultaneous analysis of mass recovery, numerical modeling, and interaction energy profiles thus suggested irreversible straining due to bacteria sizing as dominant compared to attachment to liquid-solid or liquid-air interfaces. Further studies are needed to distinguish straining mechanisms (i.e. pore structure or film straining) in different hydraulic conditions.

The effect of Piriformospora indica on the root development of maize (Zea mays L.) and remediation of petroleum contaminated soil.

As the depth of soil petroleum contamination can vary substantially under field conditions, a rhizotron experiment was performed to investigate the influence of endophyte, P. indica, on maize growth and degradation of petroleum components in a shallow and a deep-reaching subsurface layer of a soil. For control, a treatment without soil contamination was also included. The degree in contamination and the depth to which it extended had a strong effect on the growth of the plant roots. Contaminated soil layers severely inhibited root growth thus many roots preferred to bypass the shallow contaminated layer and grow in the uncontaminated soil. While the length and branching pattern of these roots were similar to those of uncontaminated treatment. Inoculation of maize with P. indica could improve root distribution and root and shoot growth in all three contamination treatments. This inoculation also enhanced petroleum degradation in soil, especially in the treatment with deep-reaching contamination, consequently the accumulation of petroleum hydrocarbons (PAHs) in the plant tissues were increased.