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A parasite-host relationship is complicated and largely remained poorly understood, especially when mixed infections involving pathogenic bacteria and viruses are present in the same host. It has been found that most parasites are able to manipulate the host's immune responses to evade or overcome its defense systems. Several mechanisms have been postulated that may explain this phenomenon in different animal species. Recent evidence suggests that coinfections involving many parasitic species alter the host's vulnerability to other microorganisms, hinder diagnostic accuracy, and may negatively impact vaccination by altering the host's immune responsiveness. The objective of this review was to provide a comprehensive summary of the current understanding of how parasites interact with other pathogens in different animal species. A better understanding of this complex relationship will aid in the improvement efforts of disease diagnosis, treatment, and control measures such as novel and effective vaccines and therapeutics for infectious diseases.
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This work investigates the effect of the alcoholic extract of Anabasis syriaca Iljin on biochemical and histological parameters in male rats. The lethal dose (50% of the plant extract) was assessed, and three separate doses (1/10th, 1/15th, and 1/20th) were orally gavaged for two weeks into three study groups of animals (five rats in each group), with one group used as a control and gavaged normal saline via the same route. Blood was collected after overnight fasting, and 24 biochemical parameters were evaluated. The gross and microscopic findings were reported after the collection of specimens from the animals and processed routinely for standard histological procedures. Among all tested biochemical parameters, a significant increase was noted in fasting serum glucose (p ≤ 0.010), troponin (p ≤ 0.001), and creatine kinase (p ≤ 0.001), while a significant decrease was found in triglycerides (p ≤ 0.001) and low-density lipoprotein (p=0.001). On the other hand, no significant histopathological lesions were present within the examined tissues of all groups. In conclusion, ethanolic extract of Anabasis syriaca negatively affected the cardiac function of male rats and increased their serum glucose but reduced their serum triglycerides and low-density lipoprotein.
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Necrotic enteritis (NE) caused by Clostridium perfringens is one of the most important enteric diseases in poultry. The antibacterial activity of two different essential oil (EO) blends against C. perfringens was investigated both in vitro and in vivo. Additionally, the immunological response to EO treatment was assessed. In the in vitro study, the antibacterial activity of EO formulas and commonly used antibiotics was evaluated against C. perfringens using disk diffusion assay, minimum inhibitory concentration (MIC) assay, and minimum bactericidal concentration (MBC) assay. In the in vivo study, NE experimental infection was performed on 440 Ross broiler chicks at 19 days of age for 4 continuous days. The chicks were treated with either EOs or amoxicillin at 22 days of age for 5 continuous days. One day after the end of treatment, the birds' performance was evaluated by calculating the feed conversion ratio. Serum samples from 120 birds were collected to measure the levels of IL-1ß, IFN-γ, IL-8, IL-10, and IL-17. After that, all birds were slaughtered, and their small intestines were subjected to gross and histopathological evaluation. In addition, bacterial counts in the small intestines were evaluated. In the in vitro study, EOs showed higher antimicrobial activities in comparison with antibiotics against C. perfringens. In the in vivo study, birds treated with EOs showed a significant decrease in bacterial counts, a significant decrease in intestinal lesions, and a significant improvement in performance compared with untreated birds (p < 0.05). Moreover, treating birds with EOs directed the immune system toward an anti-inflammatory pathway. None of the treated birds died due to NE compared with the 10% mortality rate in untreated birds. In conclusion, EOs might be an effective and safe alternative to antibiotics in the treatment of chicken NE.
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Amoxicilina/administração & dosagem , Antibacterianos/administração & dosagem , Galinhas/microbiologia , Infecções por Clostridium/tratamento farmacológico , Infecções por Clostridium/imunologia , Clostridium perfringens/efeitos dos fármacos , Enterite/tratamento farmacológico , Enterite/imunologia , Imunidade , Óleos Voláteis/administração & dosagem , Doenças das Aves Domésticas/tratamento farmacológico , Doenças das Aves Domésticas/imunologia , Ração Animal , Animais , Infecções por Clostridium/microbiologia , Infecções por Clostridium/veterinária , Enterite/patologia , Enterite/veterinária , Testes de Sensibilidade Microbiana , Necrose , Óleos Voláteis/química , Projetos Piloto , Doenças das Aves Domésticas/microbiologia , Resultado do TratamentoRESUMO
Background: Perfumes, whether original or fake, are considered of great economic value. However, overzealous use of perfumes could be associated with local or systemic side effects. Aim: This study was conducted to investigate the effects of daily use of original and fake perfumes on numbers and distribution of mast cells in several organs and tissues of Wistar rats. Methods: Three different kinds of original perfumes coded as O1, O2, and O3 and their fake counterparts coded as F1, F2, and F3 were applied once daily directly on the skin of rats for 28 consecutive days. At the end of the study, representative tissue samples were taken and processed for histopathological examination using Hematoxylin and Eosin, toluidine blue, and Wright's stains. Results: A significant (p < 0.05) elevation in mast cell count was observed in F3, O3, F1, and F2 compared to the control group. The majority of mast cells were distributed in the hepatic perivascular tissues, peribronchial and pleural tissues. There was a significant (p < 0.05) elevation in mast cell count in along the interalveolar wall, peribronchial area, and pleura tissues in F1 and O3 groups. Conclusion: Results of this study indicated that repeated use of both original and fake perfumes was associated with an increased number of mast cells in different body organs and tissues.
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Mastócitos , Perfumes , Animais , Contagem de Células , Hematoxilina , Ratos , Ratos WistarRESUMO
BACKGROUND: Human exposure to plastic and Bisphenol-A (BPA) is very wide in food or beverage containers and baby bottles that have contacted polycarbonate and epoxy resin. Currently a traditional beverage in Arabian culture is served in plastic cups instead of glass cups in different occasions. OBJECTIVE: To evaluate the possible BPA leach from the grade 5 drinking coffee plastic cups into the coffee and whether the use of plastic cups instead of glass ones to serve hot Arabian coffee poses a health risk. MATERIALS AND METHODS: Oral administration of such coffee to three different groups of rats were undertaken. Each group consisted of 10 males and 10 females. The first group (G1) was gavaged 1.5 ml of hot water in glass cups. The second group (G2) was gavaged 1.5 ml of hot water in plastic cups while the third one (G3) was gavaged 1.5 ml of Arabian coffee in plastic cups. Serum concentrations of Bisphenol-A were measured 4 weeks later. Complete postmortem examination was conducted and representative tissue samples were subjected to histopathological evaluation. RESULTS: In the control group receiving water kept in a glass cup had 24 ± 2 µg/L serum concentration and the second group was 36 ± 4 µg/L while in the third group was 29 ± 6 µg/L. No significant postmortem changes or histopathological findings were present in the studied animals. CONCLUSIONS: Bisphenol-A can be leached from food-grade plastic cups into hot drinks within short period of time, posing a significant impact to public health.
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Compostos Benzidrílicos/toxicidade , Café , Utensílios de Alimentação e Culinária , Exposição Dietética , Fenóis/toxicidade , Plásticos , Animais , Compostos Benzidrílicos/análise , Feminino , Masculino , Fenóis/análise , RatosRESUMO
Determination of the chick embryonic developmental period at which embryonic mortalities occur could help in establishing the cause of such mortalities. The late stage of embryonic development has particular importance due to its dramatic effect on life after hatching. This study was conducted to investigate the occurrence, frequency and bacterial isolates from dead-in-shell chick embryos in Northern Jordan. A total of 1,000 unhatched eggs were collected at hatching day from 10 hatcheries located in Northern Jordan. Out of 1,000 eggs, 357 (35.7%) were fertile, of which 210 (58.8%) were dead-in-shell embryos. Approximately 50.5% of the dead embryos displayed abnormalities, including neck muscles with subcutaneous petechial haemorrhages (44.3%), beak abnormalities (3.8%), eye deformities (1.9%) and anencephaly (0.5%). Sixty-six bacterial isolates were identified from 82 samples from the dead-in-shell embryos. The isolates were 22 (33.3%) Escherichia coli, 18 (27.3%) Klebsiella pneumoniae, 14 (21.2%) Staphylococcus aureus, 5 (7.6%) Pseudomonas aeruginosa, 4 (6.1%) Salmonella enteritidis, 2 (3%) Bacillus cereus and 1 (1.5%) Proteus vulgaris. Mixed growth was also recorded in 16 (19.5%) samples. There was a significant (P < 0.05) association between Escherichia coli as a bacterial isolate and the occurrence of neck and beak abnormalities. In this study, infection of check embryos with several bacterial species, particularly Escherichia coli, was identified as an important cause of multiple congenital abnormalities involving the neck and beak of unhatched chicks.
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BACKGROUND: Reference intervals (RIs) of various blood biochemical parameters are used in assessing the clinical health and nutritional status of animals. Blood biochemical analyte RIs are not currently available in Shami goats. OBJECTIVES: This study aimed to determine the RIs of selected blood biochemical analytes in Shami goats. METHODS: Blood from 120 animals (females N = 70; males N = 50) was used to determine the RIs of total protein (TP), albumin (ALB), globulin, fibrinogen (FIB), urea, creatinine (CRT), AST, ALT, ALP, GGT, glucose (GLU), calcium (Ca), sodium (Na), potassium (K), and chloride (CL) using commercially available kits. RESULTS: The RIs for selected blood biochemical analytes in Shami goats were: TP (34.9-83.5 g/L), ALB (22.3-55.1 g/L), globulin (9.9-50 g/L), FIB (2.19-5.45 g/L), urea (0.55-9.5 mmol/L), CRT (11.4-221 µmol/L), aspartate aminotransferase (7.9-299 U/L), ALT (2.3-49 U/L), ALP (7.7-950 U/L), GGT (4.5-185 U/L), GLU (1.3-6.8 mmol/L), Ca (1.65-2.6 mmol/L), Na (120-180 mmol/L), K (3.7-6.3 mmol/L), CL (91.9- 125 mmol/L), and the albumin:globulin ratio (0.45-3.9). CONCLUSIONS: The RIs of the selected blood biochemical analytes in Shami goats were determined in this study. These results can be used in the clinical diagnosis of diseases in Shami goats and to evaluate their general health and nutritional status.
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Cabras , Potássio , Animais , Aspartato Aminotransferases , Análise Química do Sangue/veterinária , Creatinina , Feminino , Masculino , Valores de ReferênciaRESUMO
Koi herpesvirus disease is a serious disease affecting both wild and common carp species in different continents throughout the world. Based on pathological and molecular findings, we document the presence of koi herpesvirus disease in Iraq as a cause of mass mortality among the common carp of the Tigris river. On a macroscopic level, the fish exhibited variably sized skin ulcerations throughout the entire trunk. The gills showed variable degrees of discoloration with an increased amount of slimy mucus. Microscopically, degeneration and necrosis with infiltration of a heterogenous population of inflammatory cells characterized different organs, primarily the skin and gills, with occasional intranuclear inclusion bodies that are consistent with koi herpesvirus disease. A semi-nested PCR assay coupled with sequencing confirmed the pathological diagnosis. Genotyping and sequence analysis of the TK gene, ORF 136 and markers I and II identified the isolated CyHV-3 as variant A1 of the Asian genotype TUSMT1 (J strain) displaying the I++II+ allele.
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PURPOSE: Investigate the possibility of delivering teriparatide orally using nanoemulsion. METHOD: Teriparatide was allowed to interact with chitosan in the presence of HPßCD.The formed polyelectrolyte complex (PEC) was characterized by DSC, FTIR, DLS and for entrapment efficiency. PEC was the incorporated in an oil phase consisting of Oleic Acid, Labrasol and Plurol Oleique to form a nanoemulsion. This preparation was characterized for refractive index, viscosity, pH, conductivity, particle size, and morphology.Bioavailability of the preparation was evaluated using rabbits against SC injection. The efficacy of the formula was tested using ovariectomized rats (an osteoporosis animal model) and mechanical and histological tests were conducted on their bones. The stability of the preparation was evaluated by storing samples at 4o C, 25o C and 40o C for three months. RESULTS: PEC testing demonstrate a complex formation with particle size of 208 nm, zeta potential of +17 mV and entrapment efficiency of 49%. For the nanoemulsion, the results demonstrate the formation of a nano-sized dispersed system (108 nm) with a drug loading of 98% and a percent protection of 90% and 71% in SGF and SIF respectively. Bioavailability results showed a sustained release profile was achieved following the oral formulation administration. Efficacy studies showed improvement in the strength, thickness and connectivity of bones. Short-term stability study demostrated that the nanoemulsion is mostly stable at 4o C. CONCLUSION: These findings demonstrate the ability of delivering Teriparatide orally using oleic acid based dispersion in combination with chitosan PEC.
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Conservadores da Densidade Óssea/administração & dosagem , Quitosana/química , Portadores de Fármacos/química , Nanopartículas/química , Ácido Oleico/química , Teriparatida/administração & dosagem , Administração Oral , Animais , Conservadores da Densidade Óssea/sangue , Conservadores da Densidade Óssea/química , Sobrevivência Celular/efeitos dos fármacos , Modelos Animais de Doenças , Composição de Medicamentos , Estabilidade de Medicamentos , Excipientes/química , Feminino , Camundongos , Células NIH 3T3 , Osteoporose/tratamento farmacológico , Tamanho da Partícula , Coelhos , Ratos Sprague-Dawley , Propriedades de Superfície , Teriparatida/sangue , Teriparatida/químicaRESUMO
The aim of this study was to determine the occurrence of on-farm risk factors and health effects associated with contamination of dairy feeds with aflatoxins (AFs), zearalenone (ZEN), trichothecenes (T-2), deoxynivalenol (DON), and fumonisins (FB) in Jordan. A pre-tested and validated questionnaire was used to determine on-farm practices and health effects associated with high levels of mycotoxins. A total of 88 feed samples were collected from the 37 farms participating in the study and analyzed using commercially available ELISA kits. The mean total AF concentration exceeded the European Union (EU) limit in alfalfa (4%) and total mixed ration (TMR) (3%) samples. Similarly, levels exceeding EU limits were observed for T-2 in alfalfa (29%), TMR (30%), and corn silage (4%). The average concentrations of ZEN and FB were 300 ppb and 11,638 ppb, respectively, which were below the EU maximum limits in all feed samples examined. Intensive management system (OR = 7.70), imported feed (OR = 3.40), feed storage on the farm for more than 1-month duration (OR = 7.90), and not using antitoxins (OR = 2.30) were significantly (P < 0.05) associated with high levels of mycotoxins in feed samples. A significant correlation (P < 0.05) was evident between the presence of mycotoxins in dairy feed and feed refusal (R = 0.70), low milk production (R = 0.50), diarrhea problems (R = 0.60), infertility (R = 0.50), and repeated breeder problems (R = 0.80). Results show that mycotoxin contamination in dairy feeds is a problem in Jordan, and appropriate measures need to be undertaken to reduce risks to human and animal health and improve production.
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Ração Animal/análise , Doenças dos Bovinos/microbiologia , Bovinos/fisiologia , Indústria de Laticínios , Fazendas , Contaminação de Alimentos/análise , Micotoxinas/análise , Animais , Doenças dos Bovinos/fisiopatologia , Ensaio de Imunoadsorção Enzimática , Feminino , Jordânia , Fatores de RiscoRESUMO
BACKGROUND AND AIM: Natural topical products have been used to enhance wound healing, especially in immunocompromised animals. The aims of this study were to evaluate and to compare the effects of moist exposed burn ointment (MEBO) and honey on the healing of full-thickness skin wounds in immunocompromised dogs. MATERIALS AND METHODS: The study was conducted using 12 adults, apparently healthy mongrel dogs. Immunosuppression was induced in six dogs by oral administration of prednisone (2 mg/kg) and azathioprine (2 mg/kg), once a day for 21 days. On each dog, a total of 9, 1.5 cm in diameter full-thickness skin circular wounds were created aseptically in the thoracolumbar area under general anesthesia using customized skin punch biopsy kit. In a random fashion, three wounds in each dog were treatment using MEBO (contains b-sitosterol, baicalin, and berberine as active ingredients in a base of beeswax and sesame oil), honey or no treatment (control), once per day for 21 days. Wounds were grossly evaluated once a day for signs of inflammation or infection. In addition, biopsy specimens and digital imaging data of each wound were obtained on days 7, 14, and 21 for histopathological evaluation of the healing process. RESULTS: Wounds in immunocompromised dogs appeared to heal significantly in a slower fashion than in non-immunocompromised counterparts. Digital analysis data showed that MEBO-treated wounds expressed better epithelialization area, faster contraction, and smaller wound area percentage when compared with honey-treated wounds. Histopathological analysis showed significantly higher angiogenesis scores in MEBO-treated wounds when compared with other treatments. CONCLUSION: Results of this study showed that MEBO resulted in significant enhancement of wound healing in both healthy and immunocompromised dogs. However, when compared to honey, the wound healing effect of MEBO was superior to that of honey.
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Foot-and-mouth disease (FMD) is a highly contagious vesicular disease that is caused by the FMD virus (FMDV). This disease affects both wild and domestic cloven-hoofed animals, and the latter of which includes cattle, swine, sheep and goats. FMD is endemic to Jordan and has a severe impact on the productivity of domestic livestock. In January 2017, FMD outbreaks were detected in different animal species across Jordan, resulting in high mortality rates among young lamb and goat populations as well as causing classic FMD symptoms in cattle. In this study, clinical specimens were collected from animals affected by FMD. The results obtained from sequencing the VP1 gene place the studied FMDV isolate within the FMDV O/ME-SA/ Ind2001e sublineage. Phylogenetic analysis of VP1 suggests that the O/JOR/1/2017 isolate is very similar to that of viruses isolated from Saudi Arabia in 2016. The possible introduction of this strain to Jordan might occur through transboundary animal movement or other transmission routes from Saudi Arabia, a neighbouring country.
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Surtos de Doenças/veterinária , Doenças Endêmicas/veterinária , Febre Aftosa/epidemiologia , Doenças das Cabras/epidemiologia , Animais , Febre Aftosa/virologia , Vírus da Febre Aftosa/isolamento & purificação , Doenças das Cabras/virologia , Cabras , Jordânia/epidemiologia , Filogenia , Arábia SauditaRESUMO
AIMS: This study aimed to provide a summary of all online available literature of published clinical and histopathological data regarding tumors affecting the reproductive tract of female sheep and goats. In addition, a detailed description of the clinical history, clinical findings, and gross and histopathological findings of one case of vaginal fibroma in an adult Awassi sheep is provided for the first time. MATERIALS AND METHODS: Internet search engines such as PubMed, ResearchGate, Scopus, ScienceDirect, and Google Scholar were used to collect all published articles in refereed journals from 2000 to 2018 regarding tumors and tumor-like lesions involving the reproductive tract of ewes and does. RESULTS: There are six published papers in sheep and nine in goats reporting various malignant and non-malignant tumors involving different parts of the reproductive tract. The most commonly diagnosed tumors of the reproductive tract were leiomyoma (six cases), adenocarcinoma (six cases), leiomyosarcoma (three cases), adenoma (one case), squamous cell carcinoma (one case), and metastatic signet ring cell carcinoma (one case). The most common sites of tumor formation were the uterus (10 cases), vulva/vagina (five cases), ovaries (four cases), cervix (four cases), and Bartholin's gland (one case). All affected animals were aged females (older than 3 years of age). In both ewes and does, the most frequently reported clinical symptoms were chronic weight loss, pyometra, hydrometra, vaginal bleeding, abnormal vaginal discharges, straining, pollakisurie, ascites, and abdominal distension. CONCLUSIONS: Tumors of the reproductive organs in sheep and goats are not uncommon and should be considered in the differential diagnoses in cases with poor reproductive function.
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Dysregulation of epidermal growth factor receptor (EGFR)/human epidermal growth factor-2 (HER2) family is a hallmark of aggressive breast cancer. Small-molecule tyrosine kinase inhibitors are among the most effective cancer targeted treatments. (-)-Oleocanthal (OC) is a naturally occurring phenolic secoiridoid lead from extra-virgin olive oil with documented anti-cancer activities via targeting mesenchymal epithelial transition factor (c-Met). Dysregulation of c-Met promotes aggressiveness to breast cancer-targeted therapies. Lapatinib (LP) is an FDA-approved dual EGFR/HER2 inhibitor for HER2-amplified breast cancer. HER2-Positive tumor cells can escape targeted therapies like LP effects by overexpressing c-Met. Combined OC-LP treatment is hypothesized to be mechanistically synergistic against HER2-overexpressing breast cancer. Combined sub-effective treatments of OC-LP resulted in synergistic anti-proliferative effects against the HER2-positive BT-474 and SK-BR-3 breast cancer cell lines, compared to OC or LP monotherapy. Antibody array and Western blot analysis showed that combined OC-LP treatment significantly inhibited EGFR, HER2, and c-Met receptor activation, as well as multiple downstream signaling proteins, compared to individual OC or LP treatment. OC-LP Combination significantly inhibited invasion and migration of breast cancer cells through reduced activation of focal adhesion kinase (FAK) and paxillin. Combined treatment of OC-10 mg/kg with LP-12.5 mg/kg suppressed more than 90% of BT-474 tumor cells growth in a nude mouse xenograft model, compared to individual OC or LP treatment. Activated c-Met, EGFR, HER2, and protein kinase B (AKT) were significantly suppressed in combination-treated mice tumors, compared to OC or LP monotherapy. This study reveals the OC future potential as combination therapy to sensitize HER2-overexpressing breast cancers and significantly reduce required doses of targeted HER family therapeutics.
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Aldeídos/administração & dosagem , Antineoplásicos/administração & dosagem , Neoplasias da Mama/tratamento farmacológico , Lapatinib/administração & dosagem , Fenóis/administração & dosagem , Receptor ErbB-2/efeitos dos fármacos , Animais , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Monoterpenos Ciclopentânicos , Quimioterapia Combinada , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Receptores ErbB/efeitos dos fármacos , Feminino , Humanos , Camundongos , Receptor ErbB-2/metabolismoRESUMO
AIM: Clinical, microbiological, molecular, and pathological assays were undertaken to characterize an outbreak of increasingly reported signs of unresponsive arthritis and pneumonia of Mycoplasma bovis infection in young calves in Jordan. MATERIALS AND METHODS: Clinical history of the affected bovine herd was investigated for the presence of respiratory and/or joint problems. Two calves with such history were clinically examined and necropsied. Representative tissues were sent for microbiological, molecular, and pathological examinations for M. bovis infection. RESULTS: The outbreak started in a herd of 220 nursing calves, 2 months before the receiving of two calves for postmortem examination. Clinically, respiratory signs and infection of one or more joints dominated in the affected calves. The morbidity and case fatality rates were 27.27% and 61.7%, respectively. The left carpal joint was markedly swollen in both calves and exhibited necrofibrinous to granulomatous arthritis in varying degrees of severity. The anteroventral lung lobes in both calves were consistently affected and revealed multifocal to coalescing severe necrogranulomatous and fibrinopurulent bronchopneumonia. Microbiological and molecular findings confirmed the pathological examination. Furthermore, bovine viral diarrhea (BVD) was diagnosed in one calf by histopathology and polymerase chain reaction. CONCLUSION: This investigation reports the first outbreak of M. bovis infection in calves located in Jordan that could occur concurrently with BVD.
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Doenças dos Bovinos/diagnóstico , Theileria/isolamento & purificação , Theileriose/diagnóstico , Animais , Biópsia por Agulha Fina/veterinária , Bovinos , Doenças dos Bovinos/parasitologia , Doenças dos Bovinos/patologia , Feminino , Linfonodos/parasitologia , Linfonodos/patologia , Theileriose/parasitologia , Theileriose/patologiaRESUMO
Chlamydophila abortus (Ch. abortus) is the etiological agent of ovine enzootic abortion (OEA) and one of the most common infectious agents of abortion in small ruminants worldwide. RFLP-PCR analysis of the outer membrane protein gene (OMP2 gene) was used for diagnosis and characterization of chlamydial causes of abortion in small ruminants in Jordan. Sixty-six placental tissues and 15 vaginal swabs were collected from aborted ewes and does to identify cause of abortion in Jordan. Thirty-eight placental samples (58 %) and 13 vaginal swabs (87 %) were positive for chlamydial DNA. Shedding of bacteria in vaginal swabs was detected within 7 days after abortion. The results of this study showed that chlamydiosis is one of the important causes of abortion in small ruminants in Jordan. In addition, vaginal swab is an excellent sample for molecular diagnosis of chlamydiosis. DNA sequencing and RFLP analysis of the OMP2 reveal that all chlamydial cause of abortion in small ruminants in Jordan are due to Ch. abortus. While, Ch. pecorum was not detected in any sample. OMP2 gene of the isolated Jordanian strain was identical (100 %) to Ch. abortus FAS strain. In conclusion, Ch. abortus is an important cause of abortion in Jordan; vaginal swab within 7 days of abortion can be used for molecular diagnosis of chlamydiosis in small ruminants.
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Aborto Animal/microbiologia , Infecções por Chlamydophila/microbiologia , Doenças das Cabras/microbiologia , Doenças dos Ovinos/microbiologia , Aborto Animal/epidemiologia , Animais , Chlamydophila/isolamento & purificação , Infecções por Chlamydophila/epidemiologia , Feminino , Doenças das Cabras/epidemiologia , Cabras , Jordânia/epidemiologia , Reação em Cadeia da Polimerase/métodos , Polimorfismo de Fragmento de Restrição , Gravidez , Ovinos , Doenças dos Ovinos/epidemiologia , Vagina/microbiologiaRESUMO
Bovine leukemia virus (BLV) is distributed worldwide. BLV has many effects on the health status and productivity of infected animals and is a potential risk for humans. In this study, we aimed to investigate the presence of and genotype bovine leukemia viruses on Jordanian dairy farms. Nested PCR coupled with RFLP and direct sequencing of a partial fragment of the env gene were carried out. Two BLV genotypes were found, genotypes 1 and 6. These genotypes were identified by nested PCR-RFLP of 444 bp of the env gene by restriction digestion with HaeIII, Bcl I and Pvu II. However, BLV-Jordan-10 seems to represent an entirely new genotype in our phylogenetic analysis. The nucleotide sequence identity between these two Jordanian BLV genotypes (1 and 6) was 96.2 %. The nucleotide sequence identity between Jordanian BLV genotype 1 and other reference BLV genotype 1 strains ranged from 99 % to 99.5 %. The nucleotide sequence similarity of the Jordanian BLV genotype 6 to other BLV genotypes ranged from 90 % to 96.7 %. A neutralizing motif and CD8(+) T-cell epitope were found in the env protein of both Jordanian isolates. In this study, we documented the presence of two BLV genotypes (1 and 6) on Jordanian dairy farms.
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Leucose Enzoótica Bovina/virologia , Vírus da Leucemia Bovina/genética , Sequência de Aminoácidos , Animais , Bovinos , Leucose Enzoótica Bovina/epidemiologia , Variação Genética , Genoma Viral , Genótipo , Jordânia/epidemiologia , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase/veterinária , Polimorfismo de Fragmento de Restrição , Alinhamento de Sequência , Proteínas do Envelope Viral/química , Proteínas do Envelope Viral/genética , Proteínas do Envelope Viral/metabolismo , Viroses/genéticaRESUMO
AIM: Chronic stress has been implicated as a contributing factor in liver injury. However, other factors that can contribute to the severity of stress effect in liver injury have not been well characterized. In this study, the combined effect of chronic psychosocial stress and variable dosing levels of vitamin C on liver injury, have been studied. METHODS: Stress was chronically induced using intruder method. Vitamin C was administered by oral gavage. Both biochemical and histopathological measures were undertaken. RESULTS: The results showed that low (50mg/kg/day) and moderate (150 mg/kg/day) doses of vitamin C alone or in combination with chronic stress had no effect on liver. However, combination of high dose of vitamin C (500 mg/kg/day) and chronic stress induced various histopathological liver lesions in most of animals in the group that was stressed and supplemented with high dose vitamin C. CONCLUSION: Results of this study show a dose-dependent effect for vitamin C in exacerbating stress contribution to liver injury.
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Ácido Ascórbico/efeitos adversos , Doença Hepática Induzida por Substâncias e Drogas/psicologia , Fígado/efeitos dos fármacos , Estresse Psicológico/fisiopatologia , Vitaminas/efeitos adversos , Administração Oral , Agressão , Animais , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/patologia , Doença Crônica , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Fígado/metabolismo , Fígado/patologia , Masculino , Fatores Desencadeantes , Ratos , Ratos Wistar , Estresse Psicológico/sangue , TerritorialidadeRESUMO
This study was done to assess the effects of Urtica dioica, Plantago major and Hypericum perforatum L herbal mixture in the MCIA rat model. In addition, a new pathological and clinical arthritis lesion assessment was developed. Sprague-Dawley (SD) rats were immunized with bovine type II collagen and muramyl dipeptide (MDP). Commercial herbal extracts were administered daily to the rats after the immunization for the course of experiment (90 days). Rats were boosted with a second collagen-MDP emulsion 60 days after the first immunization. Paws were daily evaluated macroscopically for redness, swelling, distortion, or ankylosis of the joints. On the day of sacrifice, rat paws were assessed for histopathologic changes. Herbal mixture administration decreased the clinical lesion manifestation in the MCIA rat model and led to development of similar or slightly more severe histopathological lesions compared to rats that did not receive the treatment. The clinical arthritis signs appeared as early as 13 days after the first MDP/collagen injection and with peak incidence at 20 days post-immunization. Histopathologically, animals showed changes ranging from mild to very severe. Administration of the herbal mixture used in this study had a clinical therapeutic effect on the course of the clinical manifestations in the MCIA model, but the herbal treatment had no such effect on the histopathological lesion development and even led to slightly more severe lesions. Rats in the MCIA model developed prominent clinical and histopathological changes that were comparable to rheumatoid arthritis (RA) lesions in humans.