Reduction of hexavalent chromium using Bacillus safensis isolated from an abandoned mine.

The present work focused on the isolation of a bacterial strain multi-resistant to heavy metals with a high potential for reducing hexavalent chromium (Cr(VI)) and studied its Cr(VI) removal performance in immobilized state and the mechanisms involved. Bacterial isolate was identified as Bacillus safensis CCMM B629 (B. safensis), is able to completely reduce 50, 100 and 200 mg/L of Cr(VI) after 24, 48 and 120 h, respectively under optimized conditions of pH 7 and 30°C. The coexistence of nitrates, cadmium and mercury inhibits reduction, while copper and iron significantly improve removal efficiencies. Additionally, the presence of electron donors such as glycerol, glucose and citrate significantly increases bioreduction rate. Cells immobilized in alginate beads successfully reduced Cr(VI) compared to free cells, showing the performance of biobeads in Cr(VI) reduction. Membrane fraction exhibited highest rate of Cr(VI) reduction (65%) compared to other cellular components, indicating that Cr(VI) reduction occurred primarily in cell membrane. Further characterization of Cr(VI) removal by B. safensis cells using scanning electron microscopy and energy-dispersive X-ray (SEM-EDX) analysis showed its ability to reduce and adsorb Cr(VI), confirming that hexavalent chromium was taken up successfully on bacterial cell surfaces. Based on Fourier transform infrared spectroscopy analysis (FTIR), hydroxyl, carboxyl, amide, and phosphoryl functional groups participated in combination with Cr(III). In conclusion, B. safensis is a bacterium with great potential for Cr(VI) removal, and it is a promising and competitive strain for use in bioremediation of Cr(VI) contaminated industrial effluents.

Evaluation of Bioremediation Potentiality of Bacillus mojavensis Isolated from Wastewater for the Elimination of Reactive Yellow 145 and Methyl Orange.

Textile industry waste has become one of the largest polluters in the world. In recent years, there has been a growing awareness of the need for sustainable and eco-friendly practices for the treatment of dye-laden effluents. Overall, this study highlights the potential of bioremediation as a sustainable solution for wastewater treatment. The Bacillus mojavensis isolated from wastewater and identified using 16S rRNA degraded reactive yellow 145 and methyl orange in 36 h of incubation, this decolorization was affected by pH, temperature, dye concentration, glucose concentration, source of nitrogen, type of dye, and agitation. Our study found that the optimal conditions for total decolorization of dyes were achieved by incubating B. mojavensis at 46 °C, pH 9, with 1 g/L of glucose and 2 g/L of peptone. The azoreductase activity, FT-IR analysis, and UV-visible spectrum before and after total decolorization indicated that it was a dye degradation rather than biosorption in surface Celle. In addition, the study of phytotoxicity show the metabolites of degradation are not phytotoxic in Lens esculenta seeds. In conclusion, our results suggest the use of this bacterium as an environmentally friendly and also cost-effective method, making it an attractive option for industries looking to reduce their environmental impact.

Chemical Profiling and Biological Properties of Essential Oils of Lavandula stoechas L. Collected from Three Moroccan Sites: In Vitro and In Silico Investigations.

The aim of this study was the determination of the chemical compounds of Lavandula stoechas essential oil from Aknol (LSEOA), Khenifra (LSEOK), and Beni Mellal (LSEOB), and the in vitro investigation of their antibacterial, anticandidal, and antioxidant effects, and in silico anti-SARS-CoV-2 activity. The chemical profile of LSEO was determined using GC-MS-MS analysis, the results of which showed a qualitative and quantitative variation in the chemical composition of volatile compounds including L-fenchone, cubebol, camphor, bornyl acetate, and τ-muurolol; indicating that the biosynthesis of essential oils of Lavandula stoechas (LSEO) varied depending on the site of growth. The antioxidant activity was evaluated using the ABTS and FRAP methods, our results showed that this tested oil is endowed with an ABTS inhibitory effect and an important reducing power which varies between 4.82 ± 1.52 and 15.73 ± 3.26 mg EAA/g extract. The results of antibacterial activity of LSEOA, LSEOK and LSEOB, tested against Gram-positive and Gram-negative bacteria, revealed that B. subtilis (20.66 ± 1.15-25 ± 4.35 mm), P. mirabilis (18.66 ± 1.15-18.66 ± 1.15 mm), and P. aeruginosa (13.33 ± 1.15-19 ± 1.00 mm) are the most susceptible strains to LSEOA, LSEOK and LSEOB of which LSEOB exhibits bactericidal effect against P. mirabilis. furthermore The LSEO exhibited varying degrees of anticandidal activity with an inhibition zones of 25.33 ± 0.5, 22.66 ± 2.51, and 19 ± 1 mm for LSEOK, LSEOB, and LSEOA, respectively. Additionally, the in silico molecular docking process, performed using Chimera Vina and Surflex-Dock programs, indicated that LSEO could inhibit SARS-CoV-2. These important biological properties of LSEO qualify this plant as an interesting source of natural bioactive compounds with medicinal actions.

Isotherm and kinetics modeling of biosorption and bioreduction of the Cr(VI) by Brachybacterium paraconglomeratum ER41.

Chromium is one of the most widely used metals in industry. Hexavalent form [Cr(VI)], which is found in industrial discharges, is very toxic and very soluble in water. From soil taken from an abandoned lead and iron mine, a bacterial strain capable of reducing Cr(VI) was isolated and identified as Brachybacterium paraconglomeratum ER41. Objective of this work was to evaluate the power of this bacterium to reduce Cr(VI). Results obtained showed that this bacterium is capable of eliminating 100 mg/L of Cr(VI) after 48 h (pH 8 and temperature 30 °C). For modeling biosorption kinetics, pseudo-first-order and intraparticle diffusion models gave a better fit. Furthermore, the adsorption mechanism conformed well to Langmuir's isothermal model indicating monolayer type sorption. Biomass analysis of this bacterium before and after contact with chromium by scanning electron microscopy-energy-dispersive X-ray and by Fourier transform infrared spectroscopy showed that the surface ligands of bacterial wall are probably responsible for biosorption and bioreduction process. These results suggest a potential application of B. paraconglomeratum ER41 in bioremediation of polluted discharges.

The Current State of Knowledge in Biological Properties of Cirsimaritin.

The search for natural plant-based products as new pharmacological alternatives to treat various human pathologies has taken on great importance for researchers and research laboratories. In this context, research has intensified to extract and identify natural molecules endowed with biological effects. The objective of this study is to review the source and pharmacological properties of cirsimaritin. The identification and isolation of this flavonoid from various natural sources, including medicinal plants such as Artemisia judaica, Cirsium japonicum, Lithocarpus dealbatus, Microtea debilis, and Ocimum sanctum, has been carried out and verified using different spectral techniques. Biological effect investigations are carried out with a wide variety of experimental models in vitro and in vivo and laboratory techniques. The results of these research works showed the biological properties of cirsimaritin including anticancer, antimicrobial, antidiabetic, antiparasitic, antioxidant, and anti-inflammatory effects. The mechanisms involved in the multiple activities of this molecule are diverse and include sub-cellular, cellular, and molecular levels. Indeed, this bioactive induces anti-inflammatory and antiproliferative effects by inhibiting cell membrane receptors, interference with signaling pathways, and inhibiting transcriptional factors such as Nf-κB involved in cell promotion and proliferation. In the light of these results, cirsimaritin appears as a promising and viable alternative natural bioactive drug to treat many pathological conditions.