Mevo lectin specificity toward high-mannose structures with terminal αMan(1,2)αMan residues and its implication to inhibition of the entry of Mycobacterium tuberculosis into macrophages.

Mannose-binding lectins can specifically recognize and bind complex glycan structures on pathogens and have potential as antiviral and antibacterial agents. We previously reported the structure of a lectin from an archaeal species, Mevo lectin, which has specificity toward terminal α1,2 linked manno-oligosaccharides. Mycobacterium tuberculosis expresses mannosylated structures including lipoarabinomannan (ManLAM) on its surface and exploits C-type lectins to gain entry into the host cells. ManLAM structure has mannose capping with terminal αMan(1,2)αMan residues and is important for recognition by innate immune cells. Here, we aim to address the specificity of Mevo lectin toward high-mannose type glycans with terminal αMan(1,2)αMan residues and its effect on M. tuberculosis internalization by macrophages. Isothermal titration calorimetry studies demonstrated that Mevo lectin shows preferential binding toward manno-oligosaccharides with terminal αMan(1,2)αMan structures and showed a strong affinity for ManLAM, whereas it binds weakly to Mycobacterium smegmatis lipoarabinomannan, which displays relatively fewer and shorter mannosyl caps. Crystal structure of Mevo lectin complexed with a Man7D1 revealed the multivalent cross-linking interaction, which explains avidity-based high-affinity for these ligands when compared to previously studied manno-oligosaccharides lacking the specific termini. Functional studies suggest that M. tuberculosis internalization by the macrophage was impaired by binding of Mevo lectin to ManLAM present on the surface of M. tuberculosis. Selectivity shown by Mevo lectin toward glycans with terminal αMan(1,2)αMan structures, and its ability to compromise the internalization of M. tuberculosis  in vitro, underscore the potential utility of Mevo lectin as a research tool to study host-pathogen interactions.

Supported employment programme for persons with severe mental disorders in India: A feasibility study.

BACKGROUND: A number of persons with severe mental illnesses are unable to prepare for, find or keep a job due to factors linked to their illness as well as psychosocial issues. AIM: To test the feasibility of a supported employment programme to help persons with severe mental disorders obtain and sustain employment. METHODS: A supported employment programme was developed for persons with severe mental disorders with components of (a) assessment of vocational potential, (b) vocational counselling, (c) networking and liaison with prospective employers, (d) job-related training and placement and (e) continued support for 6 months. Job placement status, social occupational functioning and disability (quantitative data) and benefits of enrolling in the employment programme (qualitative data) were assessed. RESULTS: A total of 40 employers were liaised with for providing job placement and reasonable accommodation. Out of 63 participants recruited into the study, 32 (50.8%) participants were placed in competitive jobs, placement was actively attempted for 17 (27.0%) participants, 7 (11.1%) were referred for skill training and 7 (11.1%) dropped out from the study. The disability score significantly reduced and socio-occupational functioning significantly improved in those who were placed over a period of 6 months. CONCLUSION: The supported employment programme was found to be feasible as it showed good placement rates and improvement in socio-occupational functioning and disability scores.

Development of vocational potential assessment tool and counseling module for persons with severe mental disorders.

OBJECTIVE: To develop and validate the vocational potential assessment tool and counselling module for persons with severe mental disorders (SMD). METHOD: The tool and the module were developed through review of existing assessment tools, individual interviews and focused group discussions with key stake holders- persons with SMD, caregivers, expert mental health professionals and employers. The developed tool and module were validated by experts. RESULT: The process of tool and module development was conducted simultaneously and included 11 persons with SMD, 9 caregivers, 9 experts and 9 employers. A total of 38 themes were obtained through individual interviews and focused group discussions. The developed tool and module along with a scoring key were validated by 6 mental health experts. CONCLUSION: The tool is easy to use and comprehensive, takes approximately 45 min to 1 h for assessment. The module provides a framework for vocational counselling. The scoring key provides a guideline for professionals during assessment.

Handprints of the Mind: Decoding Personality Traits and Handwritings.

CONTEXT: Handwriting analysis is a unique, specialized and emerging scientific process that has been carried out and applied for centuries now. However, its reliability and effectiveness as a method of assessing personality and behavior is not established and is still a debatable issue. The present paper aimed to examine the possibility of a correlation between clinical diagnosis and graphological analysis and to explore the key links between the underlying personality traits and its manifestations in handwriting among children. AIM: The aim was to study the possibility of a correlation between clinical diagnosis and graphological analysis. OBJECTIVES: To explore the key links between the underlying personality traits and its manifestations in handwriting among children. To study the possibility of using Graphotherapy as a remedial tool in aid of teaching/learning techniques and behavior modifications. HYPOTHESIS: There are no significant and concrete differences between the psychodiagnostic assessment of personality through Children's Personality Questionnaire (CPQ) and handwriting analysis. MATERIALS AND METHODS: N = 60, age group = 8-12 years. TOOLS: CPQ - a 16 personality factor scale and a semi-structured proforma. Simple random sampling technique was used. RESULTS: The P values for the study sample were found to be greater than 0.05 at 5% level of significance to all the 14 dimensions of personality hence going in line with the null hypothesis that states "there are no significant and concrete differences between the psychodiagnostic assessment of Personality through CPQ and handwriting analysis." Graphologists were thoroughly trained to interpret on the same 14 dimensions of personality as that of CPQ, most samples were analyzed to have a lying loop, a trait, which might also be attributed to the difference found in Trait-H, further asserting the subjective limitations of most psychological tests.

Case report on anorexia nervosa.

Anorexia nervosa is an eating disorder characterized by excessive restriction on food intake and irrational fear of gaining weight, often accompanied by a distorted body self-perception. It is clinically diagnosed more frequently in females, with type and severity varying with each case. The current report is a case of a 25-year-old female, married for 5 years, educated up to 10(th) standard, a homemaker, hailing from an upper social class Hindu (Marvadi) family, living with husband's family in Urban Bangalore; presented to our tertiary care centre with complaints of gradual loss of weight, recurrent episodes of vomiting, from a period of two years, menstrual irregularities from 1 year and amenorrhea since 6 months, with a probable precipitating factor being husband's critical comment on her weight. Diagnosis of atypical anorexia nervosa was made, with the body mass index (BMI) being 15.6. A multidisciplinary therapeutic approach was employed to facilitate remission. Through this case report the authors call for the attention of general practitioners and other medical practitioners to be aware of the symptomatology of eating disorders as most patients would overtly express somatic conditions similar to the reported case so as to facilitate early psychiatric intervention.