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1.
Braz J Biol ; 83: e270217, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37075428

RESUMO

Because of their immense economic, wellness, and remedial value, the seeds of palm (Phoenix dactylifera) were selected with synthesized silver nanoparticles (AgNPs) based on their properties for increasing the antibacterial efficacy of medical cotton. This study aimed to be contingent upon the characterisation of raw cotton fabrics treated by AgNPs with date seed extract (DSE) of P. dactylifera both individually and in combination and to investigate their antibacterial activity against various human pathogens. The prepared cotton materials with the synthesized AgNPs and/or DSE were described by both X-ray diffraction (XRD) and scanning electron microscope (SEM). At the same time, gas chromatography-mass spectrometry (GC-MS) and High-performance liquid chromatography (HPLC) were employed to determine the bioactive components in the aqueous date seed extract. The greater antibacterial activity was recorded by cotton treated with DSE and AgNPs mix, in which inhibition zones of all treatments were against Escherichia coli (8 cm), followed by Staphylococcus aureus (2.33-5.87cm) and Bacillus subtilis (2.17-4.63 cm), respectively. Overall, these findings indicate that treated cotton fabrics with synthesised AgNPs and DSE may be widely applied in various potential biological and medical applications, which could enhance environmental sustainability in closed production and consumption.


Assuntos
Nanopartículas Metálicas , Prata , Humanos , Prata/farmacologia , Prata/química , Nanopartículas Metálicas/química , Antibacterianos/farmacologia , Antibacterianos/química , Staphylococcus aureus , Extratos Vegetais/farmacologia , Extratos Vegetais/química
2.
Benef Microbes ; 14(6): 609-622, 2023 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-38350484

RESUMO

Gut microbiota may have therapeutic effects on inflammatory bowel disease (IBD). Regulating intestinal microbiota through Lactiplantibacillus plantarum (L. plantarum) and faecal microbiota transplantation (FMT) is a novel approach to treating IBD. This study aimed to explore the effect of L. plantarum and FMT pretreatment in alleviating colitis in mice. Five groups of mice (n = 6 per group) were included: CON group, DSS group (dextran sodium sulphate-induced colitis mice), LP-DSS pretreatment group (colitis mice were given strain L. plantarum and 5% DSS), DSS-FMT group (mice pretreated with faecal microbiota transplantation were given 5% DSS), and LP-FMT pretreatment group (mice pretreated with faecal microbiota transplantation and L. plantarum were given 5% DSS). Serum metabolites and intestinal microbiota were analysed by 16S rRNA sequencing liquid chromatography-mass spectrometry (LC-MS). The results demonstrated that L. plantarum and FMT improved gut microbiota in mice by increasing Firmicutes and decreasing the Bacteroidetes. In the serum metabolomics analysis, there were 11 differential metabolites in the DSS-FMT and LP-FMT pretreatment groups, and these differential metabolites were mainly glycerophospholipids and sphingolipids. It is worth noting that Lachnospira and Lactobacillus were positively associated with 8 differential metabolites. These results suggest that L. plantarum and FMT can regulate intestinal microorganisms and serum metabolomics to alleviate inflammation.


Assuntos
Colite , Microbioma Gastrointestinal , Doenças Inflamatórias Intestinais , Lactobacillus plantarum , Probióticos , Camundongos , Animais , Microbioma Gastrointestinal/fisiologia , Transplante de Microbiota Fecal , RNA Ribossômico 16S/genética , Probióticos/análise , Colite/induzido quimicamente , Colite/terapia , Doenças Inflamatórias Intestinais/terapia , Lactobacillus plantarum/fisiologia , Sulfato de Dextrana , Camundongos Endogâmicos C57BL , Modelos Animais de Doenças
3.
J Colloid Interface Sci ; 514: 59-69, 2018 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-29245073

RESUMO

Recently, the multiferroic material has fabulous attention in numerous applications owing to its excellent electronic conductivity, unique mechanical property, and higher electrocatalytic activity, etc. In this paper, we reported that the synthesis of bismuth ferrite (BiFeO3) nanosheets integrated functionalized carbon nanofiber (BiFeO3 NS/F-CNF) nanocomposite using a simple hydrothermal technique. Herein, the structural changes and crystalline property of prepared BiFeO3 NS/F-CNF nanocomposite were characterized using Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). From this detailed structural evolution, the formation of nanosheets like BiFeO3 and its nanocomposite with F-CNF were scrutinized and reported. Furthermore, the as-prepared BiFeO3 NS/F-CNF nanocomposite modified glassy carbon electrode (GCE) was applied for electrochemical detection of catechol (CC). As expected, BiFeO3 NS/F-CNF/GCE shows excellent electrocatalytic activity as well as 3.44 (F-CNF/GCE) and 7.92 (BiFeO3 NS/GCE) fold higher electrochemical redox response for CC sensing. Moreover, the proposed sensor displays a wide linear range from 0.003 to 78.02 µM with a very low detection limit of 0.0015 µM. In addition, we have validated the real-time application of our developed CC sensor in different water samples.


Assuntos
Bismuto/química , Carbono/química , Catecóis/análise , Técnicas Eletroquímicas , Compostos Férricos/química , Poluentes Químicos da Água/análise , Estrutura Molecular , Nanocompostos/química , Nanofibras/química , Tamanho da Partícula , Propriedades de Superfície
4.
Genet Mol Res ; 14(4): 11752-62, 2015 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-26436500

RESUMO

The present study explored the systematic inventory of Echinops L. (Asteraceae) of Saudi Arabia, with special reference to the molecular typing of Echinops abuzinadianus Chaudhary, an endemic species to Saudi Arabia, based on the internal transcribed spacer (ITS) sequences (ITS1-5.8S-ITS2) of nuclear ribosomal DNA. A sequence similarity search using BLAST and a phylogenetic analysis of the ITS sequence of E. abuzinadianus revealed a high level of sequence similarity with E. glaberrimus DC. (section Ritropsis). The novel primary sequence and the secondary structure of ITS2 of E. abuzinadianus could potentially be used for molecular genotyping.


Assuntos
Asteraceae/genética , DNA Espaçador Ribossômico/genética , Genótipo , Filogenia , Asteraceae/classificação , Sequência de Bases , Dados de Sequência Molecular , Tipagem Molecular/métodos , Conformação de Ácido Nucleico , Arábia Saudita , Alinhamento de Sequência , Análise de Sequência de DNA , Especificidade da Espécie
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