1.
Appl Environ Microbiol
; 56(8): 2584-2586, 1990 Aug.
Artigo
em Inglês
| MEDLINE
| ID: mdl-16348267
RESUMO
alpha-Cyclodextrin specifically inhibited raw starch digestion by Bacillus subtilis 65 alpha-amylase. The raw starch digestibility and alpha-cyclodextrin-Sepharose 6B adsorbability of this alpha-amylase were simultaneously lost when the specific domain corresponding to the affinity site essential for raw starch digestion was deleted by proteolysis. Occurrence of the affinity site on raw-starch-digesting enzymes was proven also with bacterial amylase.