RESUMO
A sensitive and specific liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MS) method was developed for the analysis of 18 drugs used for the treatment of anti-hypertension, including diuretics, calcium antagonists, and angiogenesis-converting enzyme inhibitors (ACEI) as adulterants in dietary supplements and traditional Chinese medicines. Separation was accomplished on a Xtimate C18 reversed-phase column using a mixture of methanol, acetonitrile and 20 mM ammonium formate buffer (pH 3.2) as mobile phase. The method demonstrated linearity from 0.03 to 21.52 mg kg(-1). Limits of detection ranged from 6.5 to 86.0 microg kg(-1). The recoveries of spiked samples ranged from 71% to 109%. The procedure was successfully applied in routine inspection analysis.
Assuntos
Anti-Hipertensivos/análise , Anti-Hipertensivos/química , Suplementos Nutricionais/análise , Contaminação de Medicamentos , Medicamentos de Ervas Chinesas/química , Contaminação de Alimentos , Inibidores da Enzima Conversora de Angiotensina/análise , Inibidores da Enzima Conversora de Angiotensina/química , Bloqueadores dos Canais de Cálcio/análise , Bloqueadores dos Canais de Cálcio/química , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia de Fase Reversa , Diuréticos/análise , Diuréticos/química , Controle de Medicamentos e Entorpecentes/métodos , Limite de Detecção , Reprodutibilidade dos Testes , Espectrometria de Massas por Ionização por Electrospray/métodosRESUMO
The concentration of plasma osteocalcin, or bone GLA protein (BGP), alkaline phosphatase (AKP), calcium and phosphate were measured in 142 pregnant women at various stages of pregnancy and postpartum, and also in another 47 healthy non-pregnant women as control. In contrast to the control group, plasma BGP level showed significant increase at second and third trimester (P < 0.01 and P < 0.001 respectively), with the peak level at immediate postpartum period and returned to normal in 5 days. Serum AKP level increased as pregnancy progress, and there was significant difference between the levels at the 3rd trimester and that at non-pregnant status (P < 0.01). This study demonstrated that there was marked bone metabolism changes during pregnancy, and it indicated that osteocalcin might play a role in the regulation of bone metabolism.
Assuntos
Osteocalcina/sangue , Período Pós-Parto/sangue , Gravidez/sangue , Fosfatase Alcalina/sangue , Osso e Ossos/metabolismo , Cálcio/sangue , Feminino , Humanos , Fosfatos/sangueRESUMO
The relationship between stable genome replication and the presence of species-specific H1 and H2b histones was studied in a mouse-Chinese hamster somatic cell hybrid (LMC-1). LMC-1 contained the complete chromosome complements of the mouse (LM/TK-, 46 chromosomes) and Chinese hamster (CHW-1102, 22 chromosomes) parents. No chromosome loss occurred in over a year in continuous culture, and both mouse and Chinese hamster enzymes were expressed. Chromatographic resolution of H1 histone subtypes showed that only mouse H1 genes were expressed. Resolution of chromatographically purified H2b histone variants using Triton X-100-containing gels also demonstrated that LMC-1 cells made mouse, not Chinese hamster, H2b histones. Species-specific tryptic phosphopeptides were resolved from purified H1 histones using high voltage paper electrophoresis. These studies demonstrated that characteristic mouse, but not Chinese hamster, phosphopeptides were formed in H1 histones in actively-growing LMC-1 cells. We conclude that the Chinese hamster genome may replicate in a stable manner in the presence of mouse H1 (and H2b) histones, and that there is no obligatory relationship between species-specific phosphorylation of H1 histones and stable genome replication.