Enhanced adsorption of phosphate by rice straw-based biochar prepared via metal impregnation and bio-template technology.

Phosphate removal from water through green, highly efficient technologies has received much attention. Biochar is an effective adsorbent for phosphate removal. However, adsorption capacity of phosphate on pristine rice straw-based biochar was not optimistic due to low anion exchange capacity. In this study, Fe-modified, Mg-modified and MgFe-modified rice straw-based biochar (Fe-BC, Mg-BC and MgFe-BC) were prepared by combining metal impregnation and biological template methods to improve the adsorption capacity of phosphate. The surface characteristics of biochar and the adsorption behavior of phosphate on biochar were investigated. The modified biochar had the specific surface area of 17.910-39.336 m2/g, and their surfaces were rich in a large number of functional groups and metal oxides. Phosphate release was observed on pristine rice straw-based biochar without metal impregnation. The maximum adsorption capacities of phosphate on MgFe-BC, Mg-BC and Fe-BC at 298 K were 6.93, 5.75 and 0.23 mg/g, respectively. Adsorption was a spontaneous endothermic process, while chemical adsorption dominated and electrostatic attraction and pores filling existed simultaneously. Based on the site energy distribution theory study, the standard deviation of MgFe-BC decreased from 6.96 to 4.64 kJ/mol with temperature increasing, which proved that the higher the temperature would cause the lower heterogeneity. Moreover, the effects of pH, humic acid, co-existing ions and ionic strength on phosphate adsorption of MgFe-BC were also discussed. MgFe-BC with fine pores and efficient adsorption sites is an ideal adsorbent for phosphate removal from water.

[Characteristics of microsomal phase II metabolic enzymes in mouse embryonic stem cell-derived liver tissue].

OBJECTIVE: To investigate the characteristics of phase II metabolic enzymes in mouse embryonic stem (ES) cell-derived liver tissue. METHODS: Mature hepatocytes were differentiated from embryonic stem cells in cultured mouse embryoid bodies (EB) at d18. Western blot was used to detect the expression of uridine 5'-diphosphate glucronosyl transferase (UGT1a1,UGT1a6) and microsomal glutathione S-transferases 1(mGST1) during the differentiation course.The derived liver tissue was incubated with UDPGA and 7-HFC,the formation of 7-HFC glucuronide was detected by HPLC to examine the total activities of UGT1a1 and UGT1a6. Furthermore, the microsomes were incubated with CDNB and GSH,and the mGST1 activity was measured by spectrometry. RESULTS: An increase tendency of UGT1a1 expression was noticed during the differentiation course. UGT1a6 and mGST1 were not detected in the earlier stage until d18 of differentiation. The metabolic activity of mGST1 in the derived hepatocytes was 7.65 nmol/min/mg on d18. CONCLUSION: The ES cell-derived liver tissue possesses partial metabolic function of phase II enzymes on d18 of differentiation,which might be used as a model for in vitro research on hepatic pathophysiology and phase II drug metabolism.