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1.
Br J Anaesth ; 111(6): 1004-12, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23820675

RESUMO

BACKGROUND: Pain and renal dysfunction occur in sickle cell disease. Morphine used to treat pain also co-activates platelet-derived growth factor receptor-ß (PDGFR-ß), which can adversely affect renal disease. We examined the influence of morphine in mesangial cells in vitro and in mouse kidneys in vivo. METHODS: > Mouse mesangial cells treated with 1 µM morphine in vitro or kidneys of transgenic homozygous or hemizygous sickle or control mice (n=3 for each), treated with morphine (0.75, 1.4, 2.14, 2.8, 3.6, and 4.3 mg kg(-1) day(-1) in two divided doses during the first, second, third, fourth, fifth, and sixth weeks, respectively), were used. Western blotting, bromylated deoxy uridine incorporation-based cell proliferation assay, reverse transcriptase-polymerase chain reaction, immunofluorescent microscopy, and blood/urine chemistry were used to analyse signalling, cell proliferation, opioid receptor (OP) expression, and renal function. RESULTS: Morphine stimulated phosphorylation of PDGFR-ß and mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) to the same extent as induced by platelet-derived growth factor-BB (PDGF-BB) and promoted a two-fold increase in mesangial cell proliferation. The PDGFR-ß inhibitor, AG1296, OP antagonists, and silencing of µ- and κ-OP abrogated morphine-induced MAPK/ERK phosphorylation and proliferation by ~100%. Morphine treatment of transgenic mice resulted in phosphorylation of PDGFR-ß, MAPK/ERK, and signal transducer and activator of transcription 3 (Stat3) in the kidneys. Morphine inhibited micturition and blood urea nitrogen (BUN) clearance and increased BUN and urinary protein in sickle mice. CONCLUSION: Morphine stimulates mitogenic signalling leading to mesangial cell proliferation and promotes renal dysfunction in sickle mice.


Assuntos
Analgésicos Opioides/farmacologia , Anemia Falciforme/metabolismo , Rim/efeitos dos fármacos , Morfina/farmacologia , Receptor beta de Fator de Crescimento Derivado de Plaquetas/efeitos dos fármacos , Analgésicos Opioides/administração & dosagem , Analgésicos Opioides/toxicidade , Animais , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Rim/metabolismo , Rim/fisiopatologia , Células Mesangiais/efeitos dos fármacos , Células Mesangiais/metabolismo , Camundongos , Camundongos Transgênicos , Morfina/administração & dosagem , Morfina/toxicidade , Receptor beta de Fator de Crescimento Derivado de Plaquetas/metabolismo , Receptor beta de Fator de Crescimento Derivado de Plaquetas/fisiologia , Receptores Opioides/fisiologia , Transdução de Sinais/efeitos dos fármacos , Transtornos Urinários/induzido quimicamente , Transtornos Urinários/fisiopatologia
2.
Int J Biol Macromol ; 58: 220-4, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23548861

RESUMO

In this work, the attachment, viability and functionality of rat Blood Outgrowth Endothelial Cells (rBOEC) and genetically modified rBOEC (rBOEC/eNOS-GFP), which over express endothelial nitric oxide synthase (eNOS), were investigated on Poly(lactic acid) (PLA)-chitosan and PLA-chitosan-collagen nanofibrous scaffolds. Both the cell types displayed good attachment, remained viable and functional on both scaffolds. Moreover, incorporation of collagen in the scaffold helped in sustaining the rBOEC for upto one week, although collagen was not found necessary for rBOEC/eNOS-GFP. We conclude that PLA-chitosan based nanofibrous scaffolds can be a potential candidate for BOEC based wound healing applications.


Assuntos
Quitosana/química , Células Endoteliais/fisiologia , Ácido Láctico/química , Nanofibras/química , Polímeros/química , Animais , Células Sanguíneas/fisiologia , Adesão Celular , Técnicas de Cultura de Células , Proliferação de Células , Forma Celular , Sobrevivência Celular , Células Cultivadas , Colágeno/química , Meios de Cultura , Nanofibras/ultraestrutura , Óxido Nítrico Sintase Tipo III/biossíntese , Poliésteres , Ratos , Medicina Regenerativa , Espectroscopia de Infravermelho com Transformada de Fourier , Alicerces Teciduais/química
3.
BMC Med ; 9: 2, 2011 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-21223544

RESUMO

BACKGROUND: Health disparities and the high prevalence of cardiovascular disease continue to be perplexing worldwide health challenges. This study addresses the possibility that genetic differences affecting the biology of the vascular endothelium could be a factor contributing to the increased burden of cardiovascular disease and cancer among African Americans (AA) compared to Caucasian Americans (CA). METHODS: From self-identified, healthy, 20 to 29-year-old AA (n = 21) and CA (n = 17), we established cultures of blood outgrowth endothelial cells (BOEC) and applied microarray profiling. BOEC have never been exposed to in vivo influences, and their gene expression reflects culture conditions (meticulously controlled) and donor genetics. Significance Analysis of Microarray identified differential expression of single genes. Gene Set Enrichment Analysis examined expression of pre-determined gene sets that survey nine biological systems relevant to endothelial biology. RESULTS: At the highly stringent threshold of False Discovery Rate (FDR) = 0, 31 single genes were differentially expressed in AA. PSPH exhibited the greatest fold-change (AA > CA), but this was entirely accounted for by a homolog (PSPHL) hidden within the PSPH probe set. Among other significantly different genes were: for AA > CA, SOS1, AMFR, FGFR3; and for AA < CA, ARVCF, BIN3, EIF4B. Many more (221 transcripts for 204 genes) were differentially expressed at the less stringent threshold of FDR <.05. Using the biological systems approach, we identified shear response biology as being significantly different for AA versus CA, showing an apparent tonic increase of expression (AA > CA) for 46/157 genes within that system. CONCLUSIONS: Many of the genes implicated here have substantial roles in endothelial biology. Shear stress response, a critical regulator of endothelial function and vascular homeostasis, may be different between AA and CA. These results potentially have direct implications for the role of endothelial cells in vascular disease (hypertension, stroke) and cancer (via angiogenesis). Also, they are consistent with our over-arching hypothesis that genetic influences stemming from ancestral continent-of-origin could impact upon endothelial cell biology and thereby contribute to disparity of vascular-related disease burden among AA. The method used here could be productively employed to bridge the gap between information from structural genomics (for example, disease association) and cell function and pathophysiology.


Assuntos
Negro ou Afro-Americano/genética , Suscetibilidade a Doenças , Células Endoteliais/metabolismo , Regulação da Expressão Gênica , Neoplasias/genética , Doenças Vasculares/genética , População Branca/genética , Adulto , Células Endoteliais/enzimologia , Feminino , Perfilação da Expressão Gênica , Humanos , Fatores de Transcrição Kruppel-Like/genética , Fatores de Transcrição Kruppel-Like/metabolismo , Masculino , Neoplasias/enzimologia , Neoplasias/etnologia , Análise de Sequência com Séries de Oligonucleotídeos , Monoéster Fosfórico Hidrolases/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estresse Mecânico , Doenças Vasculares/enzimologia , Doenças Vasculares/etnologia , Adulto Jovem
4.
Cancer Gene Ther ; 17(12): 855-63, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20725100

RESUMO

Endothelial cells and endothelial cell precursors encoding a therapeutic gene have induced antitumor responses in preclinical models. Culture of peripheral blood provides a rich supply of autologous, highly proliferative endothelial cells, also referred to as blood outgrowth endothelial cells (BOECs). The aim of this study was to evaluate a novel antiangiogenic strategy using BOECs expressing fms-like tyrosine kinase-1 (sFlt1) and/or angiostatin-endostatin (AE) fusion protein. Conditioned medium from BOECs expressing sFlt1 or AE suppressed in vitro growth of pulmonary vein endothelial cells by 70% compared with conditioned medium from non-transduced BOEC controls. Reverse transcriptase-PCR analysis indicated that systemically administered BOECs proliferated in tumor tissue relative to other organs in C3(1)SV40 TAG transgenic (C3TAG) mice with spontaneous mammary tumors. Tumor volume was reduced by half in C3TAG mice and in mice bearing established lung or pancreatic tumors in response to the treatment with sFlt1-BOECs, AE-BOECs or their combination. Studies of tumor vascular density confirmed that angiogenic inhibition contributed to slowed tumor growth. In an orthotopic model of glioma, the median survival of mice treated with sFlt1-BOECs was double that of mice receiving no BOEC treatment (P=0.0130). These results indicate that further research is warranted to develop BOECs for clinical application.


Assuntos
Inibidores da Angiogênese/genética , Células Endoteliais/metabolismo , Terapia Genética/métodos , Neoplasias/terapia , Neovascularização Patológica/terapia , Angiostatinas/genética , Angiostatinas/metabolismo , Animais , Células Sanguíneas/metabolismo , Linhagem Celular Tumoral , Células Cultivadas , Endostatinas/genética , Células Endoteliais/citologia , Humanos , Camundongos , Camundongos Transgênicos , Neoplasias/irrigação sanguínea , Neovascularização Patológica/patologia , Neoplasias Pancreáticas/patologia , Neoplasias Pancreáticas/terapia , Fenótipo
5.
Transfus Clin Biol ; 15(1-2): 14-8, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18501652

RESUMO

Sickle RBC are abnormally adherent to vascular endothelial cells. We briefly review the mechanisms that underlie this type of cell/cell adhesion, expose a number of extant myths about RBC adhesion, and discuss some aspects that need consideration via future experimentation. The relationship of this phenomenon of RBC-endothelial adhesion to the cytoadherence of parasitized sickle RBC is not yet clear.


Assuntos
Anemia Falciforme/fisiopatologia , Adesão Celular/fisiologia , Endotélio Vascular/fisiologia , Eritrócitos/fisiologia , Anemia Falciforme/sangue , Adesão Celular/efeitos dos fármacos , Eritrócitos/parasitologia , Humanos , Leucócitos/fisiologia , Malária Falciparum/sangue , Malária Falciparum/fisiopatologia , Reologia
6.
Br J Cancer ; 97(4): 513-22, 2007 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-17653078

RESUMO

Angiogenesis and post-natal vasculogenesis are two processes involved in the formation of new vessels, and both are essential for tumour growth and metastases. We isolated endothelial cells from human blood mononuclear cells by selective culture. These blood outgrowth cells expressed endothelial cell markers and responded correctly to functional assays. To evaluate the potential of blood outgrowth endothelial cells (BOECs) to construct functional vessels in vivo, NOD-SCID mice were implanted with Lewis lung carcinoma cells subcutaneously (s.c.). Blood outgrowth endothelial cells were then injected through the tail vein. Initial distribution of these cells occurred throughout the lung, liver, spleen, and tumour vessels, but they were only found in the spleen, liver, and tumour tissue 48 h after injection. By day 24, they were mainly found in the tumour vasculature. Tumour vessel counts were also increased in mice receiving BOEC injections as compared to saline injections. We engineered BOECs to deliver an angiogenic inhibitor directly to tumour endothelium by transducing them with the gene for human endostatin. These cells maintained an endothelial phenotype and decreased tumour vascularisation and tumour volume in mice. We conclude that BOECs have the potential for tumour-specific delivery of cancer gene therapy.


Assuntos
Inibidores da Angiogênese/genética , Carcinoma Pulmonar de Lewis/terapia , Endostatinas/genética , Endostatinas/uso terapêutico , Células Endoteliais/transplante , Terapia Genética/métodos , Neovascularização Patológica/terapia , Animais , Carcinoma Pulmonar de Lewis/irrigação sanguínea , Carcinoma Pulmonar de Lewis/patologia , Células Cultivadas , Células Endoteliais/metabolismo , Células Endoteliais/patologia , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos NOD , Camundongos Nus , Camundongos SCID , Modelos Biológicos , Transplante de Neoplasias , Fenótipo , Transfecção
7.
Tissue Eng ; 11(9-10): 1332-45, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-16259589

RESUMO

Evidence suggests that bone marrow-derived cells circulating in adult blood, sometimes called endothelial progenitor cells, contribute to neovascularization in vivo and give rise to cells expressing endothelial markers in culture. To explore the utility of blood-derived cells expressing an endothelial phenotype for creating tissue-engineered microvascular networks, we employed a three-dimensional in vitro angiogenesis model to compare microvascular network formation by human blood outgrowth endothelial cells (HBOECs) with three human vessel-derived endothelial cell (EC) types: human umbilical vein ECs (HUVECs), and adult and neonatal human microvascular ECs. Under every condition investigated, HBOECs within collagen gels elongated significantly more than any other cell type. Under all conditions investigated, gel contraction and cell elongation were correlated, with HBOECs demonstrating the largest generation of force. HBOECs did not exhibit a survival advantage, nor did they enhance elongation of HUVECs when the two cell types were cocultured. Network formation of both HBOECs and HUVECs was inhibited by blocking antibodies to alpha2beta1, but not alpha(v)beta3, integrins. Taken together, these data suggest that superior network exhibited by HBOECs relative to vessel-derived endothelial cells is not due to a survival advantage, use of different integrins, or secretion of an autocrine/paracrine factor, but may be related to increased force generation.


Assuntos
Vasos Sanguíneos , Células Endoteliais/citologia , Células Endoteliais/fisiologia , Endotélio Vascular/citologia , Neovascularização Fisiológica/fisiologia , Engenharia Tecidual/métodos , Adulto , Animais , Células da Medula Óssea/citologia , Técnicas de Cultura de Células , Morte Celular , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Técnicas de Cocultura , Colágeno/metabolismo , Relação Dose-Resposta a Droga , Combinação de Medicamentos , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Endotélio Vascular/fisiologia , Fator 2 de Crescimento de Fibroblastos/farmacologia , Géis , Humanos , Marcação In Situ das Extremidades Cortadas/métodos , Recém-Nascido , Integrinas/fisiologia , Microcirculação , Neovascularização Fisiológica/efeitos dos fármacos , Ratos , Pele/irrigação sanguínea , Pele/citologia , Acetato de Tetradecanoilforbol/farmacologia , Fatores de Tempo , Veias Umbilicais/citologia , Fator A de Crescimento do Endotélio Vascular/farmacologia , Cicatrização/efeitos dos fármacos
9.
Exp Cell Res ; 297(2): 574-84, 2004 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-15212957

RESUMO

When suspended in collagen gels, endothelial cells elongate and form capillary-like networks containing lumens. Human blood outgrowth endothelial cells (HBOEC) suspended in relatively rigid 3 mg/ml floating collagen gels, formed in vivo-like, thin, branched multi-cellular structures with small, thick-walled lumens, while human umbilical vein endothelial cells (HUVEC) formed fewer multi-cellular structures, had a spread appearance, and had larger lumens. HBOEC exert more traction on collagen gels than HUVEC as evidenced by greater contraction of floating gels. When the stiffness of floating gels was decreased by decreasing the collagen concentration from 3 to 1.5 mg/ml, HUVEC contracted gels more and formed thin, multi-cellular structures with small lumens, similar in appearance to HBOEC in floating 3 mg/ml gels. In contrast to floating gels, traction forces exerted by cells in mechanically constrained gels encounter considerable resistance. In constrained collagen gels (3 mg/ml), both cell types appeared spread, formed structures with fewer cells, had larger, thinner-walled lumens than in floating gels, and showed prominent actin stress fibers, not seen in floating gels. These results suggest that the relative magnitudes of cellular force generation and apparent matrix stiffness modulate capillary morphogenesis in vitro and that this balance may play a role in regulating angiogenesis in vivo.


Assuntos
Capilares/fisiologia , Células Endoteliais/fisiologia , Matriz Extracelular/metabolismo , Neovascularização Fisiológica , Actinas/metabolismo , Capilares/citologia , Capilares/crescimento & desenvolvimento , Linhagem Celular , Colágeno Tipo I/metabolismo , Meios de Cultura , Células Endoteliais/citologia , Endotélio Vascular/citologia , Corantes Fluorescentes , Géis , Humanos , Microscopia Confocal , Morfogênese , Rodaminas , Estresse Mecânico , Especificidade por Substrato , Tração , Veias Umbilicais/citologia
10.
J Lab Clin Med ; 138(5): 322-31, 2001 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11709656

RESUMO

Monoclonal antibody P1H12 recognizes circulating endothelial cells and endothelia of all sizes of blood vessels. To identify the protein recognized by P1H12, we expressed a cDNA library in CHO cells and sequenced the cDNA from positive cells. The P1H12 sequence was identical, except at several bases, to that reported for melanoma cell surface antigen MUC18/CD146. Aggregation assays demonstrated that CD146 mediates Ca(++)-independent homotypic endothelial cell adhesion. P1H12 mAb abrogated interactions between human microvascular endothelial cells (HMVECs) but not between human umbilical vein endothelial cells (HUVECs). P1H12 mAb abrogated P1H12-positive (CHO(P1H12))-association with HMVECs or HUVECs. CD146 distribution is sparser on HUVECs than on HMVECs. These data imply that HMVECs and HUVECs express the CD146 binding partner but that CD146 is functional (or at sufficient density) only on HMVECs. HMVEC monolayers treated with soluble P1H12 mAb showed increased permeability to albumin, with accompanying changes in actin, paxillin, FAK, and caveolin distribution and changes in tyrosine phosphorylation of FAK. Stimulation with P1H12 mAb led to redistribution of NF-kappa B to the nucleus. P1H12 mAb bound to beads inhibited closure of wounded endothelial monolayers. CD146 thus joins VE-cadherin and PECAM-1 as a molecule that mediates homotypic endothelial cell adhesion. CD146 has both structural functions and signaling functions important for endothelial monolayer integrity.


Assuntos
Antígenos CD , Antígenos de Superfície/análise , Endotélio Vascular/fisiologia , Glicoproteínas de Membrana , Moléculas de Adesão de Célula Nervosa , Animais , Anticorpos Monoclonais/imunologia , Antígenos de Superfície/genética , Antígenos de Superfície/fisiologia , Antígeno CD146 , Células CHO , Linhagem Celular , Cricetinae , Humanos , Permeabilidade , Cicatrização
11.
Am J Physiol Cell Physiol ; 281(5): C1422-33, 2001 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11600404

RESUMO

Endothelium critically regulates systemic and pulmonary vascular function, playing a central role in hemostasis, inflammation, vasoregulation, angiogenesis, and vascular growth. Indeed, the endothelium integrates signals originating in the circulation with those in the vessel wall to coordinate vascular function. This highly metabolic role differs significantly from the historic view of endothelium, in which it was considered to be merely an inert barrier. New lines of evidence may further change our understanding of endothelium, in regard to both its origin and function. Embryological studies suggest that the endothelium arises from different sites, including angiogenesis of endothelium from macrovascular segments and vasculogenesis of endothelium from microcirculatory segments. These findings suggest an inherent phenotypic distinction between endothelial populations based on their developmental origin. Similarly, diverse environmental cues influence endothelial cell phenotype, critical to not only normal function but also the function of a diseased vessel. Consequently, an improved understanding of site-specific endothelial cell function is essential, particularly with consideration to environmental stimuli present both in the healthy vessel and in development of vasculopathic disease states. The need to examine endothelial cell phenotypes in the context of vascular function served as the basis for a recent workshop sponsored by the National Heart, Lung, and Blood Institute (NHLBI). This report is a synopsis of pertinent topics that were discussed, and future goals and research opportunities identified by the participants of the workshop are presented.


Assuntos
Endotélio Vascular/patologia , Cardiopatias/patologia , Doenças Hematológicas/patologia , Pneumopatias/patologia , Animais , Comunicação Celular/fisiologia , Endotélio Vascular/citologia , Cardiopatias/sangue , Doenças Hematológicas/sangue , Humanos , Pneumopatias/sangue , Fenótipo
13.
Thromb Haemost ; 85(2): 250-5, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11246542

RESUMO

Using a novel whole blood assay, we recently demonstrated that tissue factor procoagulant activity (TF PCA) is present in normal individuals. Preliminary experiments suggested that this activity is localized in the mononuclear cell fraction. Postulating that whole blood TF PCA would therefore be undetectable when monocytes and neutrophils are absent from peripheral blood, we assayed TF PCA during the peri-transplant period in 15 consecutive patients undergoing allogeneic (n = 12) or autologous (n = 3) bone marrow transplantation (BMT) or peripheral blood stem cell transplantation (PBSCT). Baseline (pre-transplant) mean TF PCA was higher in patients compared to normal controls (P <0.005). Unexpectedly, although TF PCA during the period of profound aplasia was significantly reduced compared to baseline (p <0.05), fully 55% of the initial activity remained detectable. During the engraftment phase, TF PCA returned to pre-transplant levels, with a linear correlation between monocyte counts and TF PCA (r = 0.63). In contrast to normal whole blood, incubation of aplastic samples with E. Coli lipopolysaccharide ex vivo failed to induce TF PCA. Throughout the period of study--but especially during the aplastic phase--the absolute number of circulating endothelial cells (CECs) that were TF antigen-positive was increased compared to normals (P <0.001). However, removal of these cells from whole blood samples failed to significantly diminish total TF PCA indicating that CECs alone could not account for the detectable TF PCA during aplasia. We conclude that neither circulating mature myelo-monocytic cells nor endothelial cells can account for all the functionally intact TF in peripheral blood. Further studies are needed to identify the other source(s) of TF PCA.


Assuntos
Medula Óssea/anormalidades , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Hemostáticos/sangue , Tromboplastina/metabolismo , Adolescente , Adulto , Testes de Coagulação Sanguínea , Transplante de Medula Óssea/efeitos adversos , Criança , Pré-Escolar , Endotélio Vascular/citologia , Endotélio Vascular/metabolismo , Feminino , Humanos , Contagem de Leucócitos , Masculino , Pessoa de Meia-Idade , Monócitos/metabolismo , Neutrófilos/metabolismo , Fatores de Tempo
14.
Am J Pathol ; 158(3): 893-903, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11238038

RESUMO

Chronic nephropathy is a recognized complication of sickle cell disease. Using a transgenic sickle mouse, we examined whether oxidative stress occurs in the sickle kidney, the origins and functional significance of such oxidant stress, and the expression of the oxidant-inducible, potentially protective gene, heme oxygenase-1 (HO-1); we also examined the expression of HO-1 in the kidney and in circulating endothelial cells in sickle patients. We demonstrate that this transgenic sickle mouse exhibits renal enlargement, medullary congestion, and a reduced plasma creatinine concentration. Oxidative stress is present in the kidney as indicated by increased amounts of lipid peroxidation; heme content is markedly increased in the kidney. Exacerbation of oxidative stress by inhibiting glutathione synthesis with buthionine-sulfoximine dramatically increased red blood cell sickling in the sickle kidney: in buthionine-sulfoximine-treated sickle mice, red blood cell sickling extended from the medulla into the cortical capillaries and glomeruli. HO activity is increased in the sickle mouse kidney, and is due to induction of HO-1. In the human sickle kidney, HO-1 is induced in renal tubules, interstitial cells, and in the vasculature. Expression of HO-1 is increased in circulating endothelial cells in patients with sickle cell disease. These results provide the novel demonstration that oxidative stress occurs in the sickle kidney, and that acute exacerbation of oxidative stress in the sickle mouse precipitates acute vaso-occlusive disease. Additionally, the oxidant-inducible, heme-degrading enzyme, HO-1, is induced regionally in the murine and human sickle kidney, and systemically, in circulating endothelial cells in sickle patients.


Assuntos
Anemia Falciforme/enzimologia , Heme Oxigenase (Desciclizante)/biossíntese , Rim/enzimologia , Estresse Oxidativo , Anemia Falciforme/sangue , Anemia Falciforme/patologia , Animais , Butionina Sulfoximina/farmacologia , Creatinina/sangue , Endotélio Vascular/enzimologia , Inibidores Enzimáticos/farmacologia , Eritrócitos/patologia , Glutationa/biossíntese , Heme/metabolismo , Heme Oxigenase (Desciclizante)/genética , Heme Oxigenase-1 , Humanos , Rim/irrigação sanguínea , Rim/patologia , Peroxidação de Lipídeos , Proteínas de Membrana , Camundongos , Camundongos Transgênicos , Ativação Transcricional
15.
Blood ; 97(7): 1937-41, 2001 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-11264155

RESUMO

The vessel wall endothelium undoubtedly plays a role in the vascular pathobiology of sickle cell disease. This pilot study tested the feasibility of using an inhibitor of nuclear factor (NF)-kappa B, a transcription factor, to modify the endothelial activation state of patients with this vascular disease. For a total of 7 separate drug exposure tests, 3 subjects with sickle cell disease took sulfasalazine (given orally at 1 g every 8 hours), and the activation state of their circulating endothelial cells (CECs) was assessed using immunofluorescence microscopy. Companion studies were also performed using sulfasalazine in sickle transgenic mice to verify its effect simultaneously on both CECs and vessel wall endothelium. Both CECs and tissue vessel wall endothelium in sickle mice have an activated phenotype. In these mice sulfasalazine significantly reduced CEC expression of vascular cell adhesion molecule (VCAM), intracellular adhesion molecule (ICAM), and E-selectin, and it correspondingly reduced expression of these molecules in some tissue vessels. In humans with sickle cell disease, sulfasalazine significantly reduced CEC expression of VCAM, ICAM, and E-selectin, but it did not reduce expression of tissue factor. Addition of a second transcription factor inhibitor, salsalate, did not change this result. This pilot study suggests that endothelial cell activation state can be modified and down-regulated in vivo by sulfasalazine. (Blood. 2001;97:1937-1941)


Assuntos
Anemia Falciforme/tratamento farmacológico , Anti-Inflamatórios não Esteroides/uso terapêutico , Endotélio Vascular/efeitos dos fármacos , Sulfassalazina/uso terapêutico , Adulto , Anemia Falciforme/metabolismo , Anemia Falciforme/patologia , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Avaliação de Medicamentos , Selectina E/biossíntese , Selectina E/genética , Endotélio Vascular/metabolismo , Endotélio Vascular/patologia , Estudos de Viabilidade , Feminino , Ácido Fólico/uso terapêutico , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Molécula 1 de Adesão Intercelular/biossíntese , Molécula 1 de Adesão Intercelular/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Projetos Piloto , Segurança , Salicilatos/farmacologia , Salicilatos/uso terapêutico , Sulfassalazina/farmacologia , Tromboplastina/biossíntese , Tromboplastina/genética , Transcrição Gênica/efeitos dos fármacos , Molécula 1 de Adesão de Célula Vascular/biossíntese , Molécula 1 de Adesão de Célula Vascular/genética
16.
Am J Hematol ; 65(4): 281-4, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11074553

RESUMO

Desferrioxamine (DFO) is an important iron-chelating agent. It has also been thought of as an agent with anti-oxidant potential as it chelates ferric iron in various parts of the body. However, there is evidence suggesting that it may paradoxically affect red blood cells (RBCs) by inducing intracellular oxidant stress. Recently we observed that incubation of RBCs with DFO decreases NAD redox potential in normal RBC. To further understand the mechanism of DFO's interaction with RBC, we conducted a study to determine the effect of extracellular DFO upon RBC's redox status. We examined NAD redox potential in intact RBC (N = 7) incubated with DFO conjugated to starch. RBCs were incubated with 4 mM DFO for 3(1/2) hr and with 6 mM DFO for 2 and 3(1/2) hr. Significant decreases in NAD redox potential were observed after the incubations. With 4 mM DFO at the 3 (1/2) hr time point the mean decrease was 12.37% +/- 9.96% (P < 0.0085). With 6 mM DFO, the mean decreases were 18.54% +/- 9.79% (P < 0.0013) and 19.16% +/- 8.78% (P < 0.0006) for the 2 and 3 (1/2) hr incubations, respectively. DFO by itself is very poorly permeable to RBC. Conjugation with starch further ensured impermeability of DFO. The data presented here confirm the oxidant effect of DFO on RBC. The data also demonstrate that the effect of DFO on RBC's NAD redox potential originates extracellularly.


Assuntos
Quelantes/farmacologia , Desferroxamina/farmacologia , Eritrócitos/efeitos dos fármacos , Eritrócitos/metabolismo , NAD/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Amido/farmacologia , Adulto , Humanos , Oxirredução
17.
Am J Physiol Regul Integr Comp Physiol ; 279(6): R1949-55, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11080057

RESUMO

We investigated a transgenic mouse model of sickle cell disease, homozygous for deletion of mouse beta-globin and containing transgenes for human beta(S) and beta(S-antilles) globins linked to the transgene for human alpha-globin. In these mice, basal cGMP production in aortic rings is increased, whereas relaxation to an endothelium-dependent vasodilator, A-23187, is impaired. In contrast, aortic expression of endothelial nitric oxide synthase (NOS) is unaltered in sickle mice, whereas expression of inducible NOS is not detected in either group; plasma nitrate/nitrite concentrations and NOS activity are similar in both groups. Increased cGMP may reflect the stimulatory effect of peroxides (an activator of guanylate cyclase), because lipid peroxidation is increased in aortae and in plasma in sickle mice. Despite increased vascular cGMP levels in sickle mice, conscious systolic blood pressure is comparable to that of aged-matched controls; sickle mice, however, evince a greater rise in systolic blood pressure in response to nitro-L-arginine methyl ester, an inhibitor of NOS. Systemic concentrations of the vasoconstrictive oxidative product 8-isoprostane are increased in sickle mice. We conclude that vascular responses are altered in this transgenic sickle mouse and are accompanied by increased lipid peroxidation and production of cGMP; we suggest that oxidant-inducible vasoconstrictor systems such as isoprostanes may oppose nitric oxide-dependent and nitric oxide-independent mechanisms of vasodilatation in this transgenic sickle mouse. Destabilization of the vasoactive balance in the sickle vasculature by clinically relevant states may predispose to vasoocclusive disease.


Assuntos
Anemia Falciforme/fisiopatologia , Globinas/genética , Hemoglobina Falciforme/genética , Músculo Liso Vascular/fisiopatologia , Anemia Falciforme/genética , Animais , Aorta/fisiologia , Aorta/fisiopatologia , Autoanticorpos/genética , Pressão Sanguínea , Calcimicina/farmacologia , GMP Cíclico/metabolismo , Modelos Animais de Doenças , Endotélio Vascular/fisiologia , Endotélio Vascular/fisiopatologia , Globinas/deficiência , Hemoglobina Falciforme/imunologia , Humanos , Técnicas In Vitro , Peroxidação de Lipídeos , Camundongos , Camundongos Transgênicos , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/fisiologia , Nitratos/sangue , Óxido Nítrico Sintase/metabolismo , Óxido Nítrico Sintase Tipo II , Óxido Nítrico Sintase Tipo III , Nitritos/sangue , Papaverina/farmacologia , Vasoconstrição/efeitos dos fármacos , Vasoconstrição/fisiologia , Vasodilatação/efeitos dos fármacos , Vasodilatação/fisiologia
18.
Blood ; 96(7): 2451-9, 2000 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-11001897

RESUMO

Sickle cell anemia is characterized by painful vaso-occlusive crises. It is hypothesized that monocytes are activated in sickle cell disease and can enhance vaso-occlusion by activating endothelium. To test this hypothesis, human umbilical vein endothelial cells (HUVEC) and human microvascular endothelial cells (MVEC) with sickle and normal mononuclear leukocytes were incubated, and endothelial activation was measured. Endothelial cells incubated with sickle mononuclear leukocytes were more activated than those incubated with normal mononuclear leukocytes, as judged by the increased endothelial expression of adhesion molecules and tissue factor and the adhesion of polymorphonuclear leukocytes (PMNL). Monocytes, not lymphocytes or platelets, were the mononuclear cells responsible for activating endothelial cells. Sickle monocytes triggered endothelial nuclear factor-kappa B (NF-kappaB) nuclear translocation. Cell-to-cell contact of monocytes and endothelium enhanced, but was not required for, activation. Antibodies to tumor necrosis factor-alpha (TNF-alpha) and interleukin-1-beta (IL-1beta) blocked activation of the endothelium by monocytes. Peripheral blood monocytes from patients with sickle cell disease had 34% more IL-1beta (P =.002) and 139% more TNF-alpha (P =.002) per cell than normal monocytes. Sixty percent of sickle monocytes expressed the adhesion molecule ligand CD11b on their surfaces compared with only 20% of normal monocytes (P =.002). Serum C-reactive protein, a marker of systemic inflammation, was increased 12-fold in sickle serum than in normal serum (P =.003). These results demonstrate that sickle monocytes are activated and can, in turn, activate endothelial cells. It is speculated that vascular inflammation, marked by activated monocytes and endothelium, plays a significant role in the pathophysiology of vaso-occlusion in sickle cell anemia.


Assuntos
Anemia Falciforme/fisiopatologia , Endotélio Vascular/fisiopatologia , Leucócitos Mononucleares/fisiologia , Monócitos/fisiologia , Sítios de Ligação , Adesão Celular , DNA/metabolismo , Selectina E/genética , Feminino , Expressão Gênica , Humanos , Molécula 1 de Adesão Intercelular/genética , Interleucina-1/fisiologia , Antígeno de Macrófago 1/análise , Masculino , Microcirculação , Monócitos/imunologia , NF-kappa B/metabolismo , Neutrófilos/fisiologia , RNA Mensageiro/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Tromboplastina/genética , Fator de Necrose Tumoral alfa/fisiologia , Veias Umbilicais , Molécula 1 de Adesão de Célula Vascular/genética
19.
J Clin Invest ; 106(3): 411-20, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10930444

RESUMO

In sickle cell anemia, the initiation, progression, and resolution of a vasoocclusive episode may present features of ischemia-reperfusion injury, with recurrent episodes of ischemia/hypoxia and reoxygenation promoting inflammation. Here, we have tested the hypothesis that hypoxia/reoxygenation triggers inflammation in the transgenic sickle mouse. In these mice, even at ambient air, peripheral leukocyte counts are elevated by 1.7-fold and neutrophil counts by almost 3-fold. Two hours of hypoxia, followed by reoxygenation, induced a greater than normal rolling flux and adhesion of leukocytes in these mice, but no leukocyte extravasation. When 3 hours of hypoxia was followed by reoxygenation, sickle mice, but not normal mice, showed a distinct inflammatory response characterized by an increased number of adherent and emigrated leukocytes. Because these events, which are exaggerated in sickle mice, are not seen in response to hypoxia alone, we conclude that they represent a form of reperfusion injury. Studies using an H(2)O(2)-sensitive probe revealed clear evidence of oxidant production in vascular endothelial cells after hypoxia/reoxygenation in sickle mice. Infusion of an anti-P-selectin antibody, but not an anti-E-selectin antibody, completely inhibited this inflammatory response and significantly increased wall shear rates. These findings suggest that leukocyte-endothelium interaction contribute to vasoocclusive events in the sickle mice and perhaps in human sickle disease.


Assuntos
Anemia Falciforme/complicações , Inflamação/etiologia , Traumatismo por Reperfusão/complicações , Anemia Falciforme/sangue , Anemia Falciforme/fisiopatologia , Animais , Anticorpos Monoclonais/farmacologia , Adesão Celular , Movimento Celular , Modelos Animais de Doenças , Selectina E/imunologia , Selectina E/fisiologia , Hemodinâmica , Humanos , Inflamação/sangue , Inflamação/fisiopatologia , Contagem de Leucócitos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Mutantes , Camundongos Transgênicos , Neutrófilos , Oxidantes/metabolismo , Selectina-P/imunologia , Selectina-P/fisiologia , Traumatismo por Reperfusão/sangue , Traumatismo por Reperfusão/fisiopatologia
20.
Blood ; 96(1): 314-20, 2000 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-10891467

RESUMO

Reperfusion of tissues after interruption of their vascular supply causes free-radical generation that leads to tissue damage, a scenario referred to as "reperfusion injury." Because sickle disease involves repeated transient ischemic episodes, we sought evidence for excessive free-radical generation in sickle transgenic mice. Compared with normal mice, sickle mice at ambient air had a higher ethane excretion (marker of lipid peroxidation) and greater conversion of salicylic acid to 2,3-dihydroxybenzoic acid (marker of hydroxyl radical generation). During hypoxia (11% O(2)), only sickle mice converted tissue xanthine dehydrogenase to oxidase. Only the sickle mice exhibited a further increase in ethane excretion during restitution of normal oxygen tension after 2 hours of hypoxia. Only the sickle mice showed abnormal activation of nuclear factor-kappaB after exposure to hypoxia-reoxygenation. Allopurinol, a potential therapeutic agent, decreased ethane excretion in the sickle mice. Thus, sickle transgenic mice exhibit biochemical footprints consistent with excessive free-radical generation even at ambient air and following a transient induction of enhanced sickling. We suggest that reperfusion injury physiology may contribute to the evolution of the chronic organ damage characteristic of sickle cell disease. If so, novel therapeutic approaches might be of value.


Assuntos
Anemia Falciforme/fisiopatologia , Hemoglobina Falciforme/genética , Traumatismo por Reperfusão/fisiopatologia , Alopurinol/uso terapêutico , Anemia Falciforme/tratamento farmacológico , Anemia Falciforme/genética , Animais , Biomarcadores , Inibidores Enzimáticos/uso terapêutico , Etano/análise , Humanos , Hidroxibenzoatos/análise , Radical Hidroxila/metabolismo , Rim/metabolismo , Peroxidação de Lipídeos , Fígado/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , NF-kappa B/metabolismo , Valores de Referência , Ácido Salicílico/farmacocinética , Xantina Desidrogenase/metabolismo , Xantina Oxidase/metabolismo
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