Characteristics of Pickering emulsions stabilized by microgel particles of five different plant proteins and their application.

Pickering emulsions stabilized by protein particles are of great interest for use in real food systems. This study was to investigate the properties of microgel particles prepared from different plant proteins, i.e., soybean protein isolate (SPI), pea protein isolate (PPI), mung bean protein isolate (MPI), chia seed protein isolate (CSPI), and chickpea protein isolate (CPI). MPI protein particles had most desirable Pickering emulsion forming ability. The particles of SPI and PPI had similar particle size (316.23 nm and 294.80 nm) and surface hydrophobicity (2238.40 and 2001.13) and emulsion forming ability, while the CSPI and CPI particle stabilized emulsions had the least desirable properties. The MPI and PPI particle stabilized Pickering emulsions produced better quality ice cream than the one produced by SPI particle-stabilized emulsions. These findings provide insight into the properties of Pickering emulsions stabilized by different plant protein particles and help expand their application in emulsions and ice cream.

Freeze-thaw stability of Pickering emulsion stabilized by modified soy protein particles and its application in plant-based ice cream.

Pickering emulsions are of great interest to the food industry and their freeze-thaw stability important when used in frozen foods. Particles of soybean isolate (SPI) were heat treated and then crosslinked with transglutaminase (TG) enzyme to produce Pickering emulsions. The protein particles produced using unheated and uncrosslinked SPI (NSPI) was used as the benchmark. The mean particle size, absolute zeta potential, and surface hydrophobicity of protein particles produced using heat treatment and TG crosslinking (at 40 U/g) SPI (HSPI-TG-40) were the highest and substantially higher than those produced using NSPI. The thermal treatment of protein particles followed by crosslinking with TG enzyme improved the freeze-thaw stability of Pickering emulsions stabilized by them. The Pickering emulsions produced using HSPI-TG-40 had the lowest temperature for ice crystal formation and they had better freeze-thaw stability. The plant-based ice cream prepared by HSPI-TG-40 particle-stabilized Pickering emulsions had suitable texture and freeze-thaw stability compared to the ice cream produced using NSPI. The Pickering particles produced using heat treatment of SPI followed by crosslinking with TG (at 40 U/g) produced the most freeze-thaw stable Pickering emulsions. These Pickering particles and Pickering emulsions could be used in frozen foods such as ice cream.

Effect of Physical Modifications on Physicochemical and Functional Properties of Walnut Protein.

Walnut protein is a high-quality vegetable protein with promising applications in the food industry; however, its potential is hindered by low solubility and associated properties. We utilized various physical modification techniques (cold plasma; ball milling; superfine grinding; ultrasound; wet ball milling; and high-pressure microjet) to enhance walnut proteins' physicochemical and functional properties. The changes in particle size, microstructure, surface hydrophobicity, fluorescence, solubility, foaming, and emulsification were investigated. Cold plasma and ultrasound treatments minimally affected particle size and morphology. Cold plasma increased the particle size D4,3 from 145.20 µm to 152.50 µm. Ultrasonication reduced the particle size D4,3 to 138.00 µm. The variation was within ±10 µm, while the particle size of walnut protein significantly decreased after the other four modification treatments. The greatest variation in particle size was in the superfine grinding, with the D4,3 being reduced to 23.80 µm. Ultrasound treatment converted the ß-sheet into an α-helix, while the other methods transformed the α-helix into a ß-sheet. The dispersion stability notably improved after wet ball milling and high-pressure microjet treatments, which was accompanied by a significant increase in solubility from 6.9% (control) to 13.6% (wet ball milling) and 31.7% (high-pressure microjet). The foaming and emulsification properties were also enhanced through these modifications (foaming improved from 47% to 55.33% and emulsification improved from 4.32 m2/g to 8.27 m2/g). High-pressure microjet treatment proved most effective at improving solubility in the functional properties of walnut protein. These findings are expected to help broaden the potential utilization of walnut protein in the food industry, including in beverages and emulsions.

Natural Amphiphilic Shellac Nanoparticle-Stabilized Novel Pickering Emulsions with Droplets and Bi-continuous Structures.

Shellac is a natural amphiphilic substance, and its nanoparticles can be used to stabilize Pickering emulsions with droplets and bi-continuous structures. In this study, shellac nanoparticles (SNPs) were produced through the anti-solvent method, and these SNPs were used to produce a series of Pickering emulsions. Fourier transform infrared results showed that SNPs were generated through hydrogen bonding and hydrophobic effects. The contact angle of SNPs was 122.3°, indicating that hydrophobicity was their dominant characteristic. According to the results of confocal laser scanning microscopy, the Pickering emulsions stabilized by SNPs showed oil-in-water, bi-continuous structure, and water-in-oil characteristics, which were dependent on the oil-phase content. The resistance value of the emulsified part of these Pickering emulsion systems significantly increased at an oil-phase ratio of 80-90% (more than 105 MΩ), as compared with the 10-70% oil-phase content (around 1 MΩ). The viscosity of SNP-stabilized Pickering emulsions with bi-continuous structures was highest at 40% oil-phase content. The porous material prepared by using Pickering emulsions with bi-continuous structures as a template had an interconnected structure and was able to absorb both water and oil. This study indicated that these amphiphilic SNPs readily form bi-continuous structures and effectively stabilize Pickering emulsions with droplets. These SNPs are expected to have increased application in food and pharmaceutical industries.