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Synthesis of a bulky anilidophosphine ligand (short PNTerph) and its lanthanum complexes 1 and 3 is reported. When exposed to KPHMes, both complexes form the first example of a bis-phosphanido-phosphinidene complex 2. This complex undergoes Phospha-Wittig type reactions and its reactivity towards strong bases is further investigated.
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Foreign Body Reaction (FBR) is a critical issue to be addressed when polyethylene terephthalate (PET) textile implants are considered in the medical field to treat pathologies involving hernia repair, revascularization strategies in arterial disease, and aneurysm or heart valve replacement. The natural porosity of textile materials tends to induce exaggerated tissue ingrowth which may prevent the implants from remaining flexible. One hypothesized way to limit the FBR process is to increase the material surface roughness at the yarn level. Supercritical N2 (ScN2) jet particle projection is a technique that provides enough velocity to particles in order to induce plastic deformation on the impacted surface. This work investigates the influence of ScN2 jet projection parameters like standoff distance or particle size on the roughness that can be obtained on medical polymer yarns of various diameters (100 and 400 µm) and woven textile surfaces obtained from a 100 µm yarn. Moreover, the mechanical and biological performances of the obtained modified textile material are assessed. Results bring out that with appropriate testing conditions (500 bars jet/500 mm distance between nozzle and PET textile) and particle size around 50 µm, it is possible to generate 20 µm large and 4 µm deep craters on a 100 µm monofilament PET yarn and fabric. Regarding the strength of the textile material, it is only slightly modified with the treatment process, as the tenacity of the yarns decreases by only 10%. Moreover, It is shown that the obtained structures tend to limit the adhesion and slow down the proliferation of human fibroblasts.
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Materiais Biocompatíveis , Polímeros , Humanos , Porosidade , Próteses e Implantes , TêxteisRESUMO
OBJECTIVE/BACKGROUND: Laser in situ fenestration (LISF) is emerging as an immediately available alternative in the endovascular treatment of complex aortic aneurysm. However, its biomechanical features remain poorly understood. The aim of this study was to experimentally evaluate textile damage secondary to LISF and to compare LISF with mechanical in situ fenestration (MISF). METHODS: An in vitro study evaluated the damage created by LISF on endograft fabrics versus MISF using a needle. Five different models of commercially available aortic endografts were used (32 samples of polyethylene terephthalate and expanded polytetrafluoroethylene fabrics). Tensile strength tests were performed on the fabrics before and after in situ fenestration, to determine the loss of mechanical strength. Integral water permeability tests at the stent-fenestration interface evaluated the watertightness of junctions. Stability of the connection was assessed with a fatigue bench test flexing the branch on the fenestration. In a second step, an in vivo study evaluating LISF in sheep was conducted. RESULTS: Resulting holes had circular and cauterised edges following LISF, whereas fabric filaments were pushed aside after MISF. Tensile tests demonstrated a 34% and a 27% mechanical resistance loss after LISF (p = .004) and MISF (p = .001) compared with non-fenestrated samples. A non-significant global decrease of 7% in mechanical resistance was found following LISF compared with MISF (p = .520). Water permeability tests highlighted that leak rates were higher following LISF than with MISF with regard to multifilament specimens (p < .05). Fatigue tests induced modification of the morphology of fenestrations. The surface area of the fenestration was increased for all samples after 170,000 cycles. Regarding the in vivo study, 14 LISF were performed in 12 sheep with a technical success rate of 88%. CONCLUSION: This study demonstrates that both LISF and MISF create substantial damage to all available endograft fabrics. Until comparisons with reinforced fenestrations are performed, LISF and MISF should not be used outside investigational studies.
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Aorta Abdominal/cirurgia , Aorta Torácica/cirurgia , Implante de Prótese Vascular/instrumentação , Prótese Vascular , Procedimentos Endovasculares/instrumentação , Desenho de Prótese , Animais , Aorta Abdominal/patologia , Aorta Abdominal/fisiopatologia , Aorta Torácica/patologia , Aorta Torácica/fisiopatologia , Fenômenos Biomecânicos , Análise de Falha de Equipamento , Hemodinâmica , Humanos , Teste de Materiais , Modelos Animais , Permeabilidade , Polietilenotereftalatos , Politetrafluoretileno , Falha de Prótese , Carneiro Doméstico , Estresse Mecânico , Resistência à TraçãoAssuntos
Implante de Prótese Vascular/instrumentação , Prótese Vascular , Remoção de Dispositivo , Procedimentos Endovasculares/instrumentação , Falha de Prótese , Stents , Implante de Prótese Vascular/efeitos adversos , Procedimentos Endovasculares/efeitos adversos , Análise de Falha de Equipamento , Humanos , Teste de Materiais , Desenvolvimento de Programas , Desenho de Prótese , Resultado do TratamentoRESUMO
OBJECTIVE/BACKGROUND: The goal of the present study was to assess the aging phenomena on second-generation textile endoprostheses (EPs) through explant analysis and to establish a preliminary classification of observed defects and material damages. METHODS: From January 2011 to June 2016 110 second- and recent-generation EPs were collected as a part of a European collaborative retrieval program. The analysis focused on the first 41 consecutive commercial EPs collected between 2011 and 2014 and made from polyethylene terephthalate. Explants were submitted to a standardized evaluation protocol, which included data recording, eye-naked evaluation, cleaning of organic remnants, and structural analysis under numerical optical microscopy. Observations were reported using a classification based on 15 features evaluating the fabric, the stitches between the fabric and the stents, and the stents. The total surface area of the holes within the fabric was measured. RESULTS: EPs were implanted for thoracic and abdominal procedures in 12 and 29 cases, respectively. The mean ± SD duration of implantation was 34 ± 26 months (range 2 days-8 years). Sixty-four percent of the samples demonstrated at least one defect caused by compression damage potentially related to the insertion of the EP within the delivery system, which promoted holes and tears. Ninety-five percent of all EPs demonstrated at least one type of abrasion on the stitches. The degradation of the stitches and the number of ruptures increased with duration of implantation. Stent degradation was rare and consisted of corrosion and rupture. Cumulated holed surface area increased with time and was measured up to 13.5 mm2. CONCLUSION: Various aging-related phenomena on commercial textile EPs were identified and classified. Main damaging mechanisms were related to compression and abrasion leading to tears and holes in the fabric and rupture of stitches.
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Implante de Prótese Vascular/instrumentação , Prótese Vascular , Remoção de Dispositivo , Procedimentos Endovasculares/instrumentação , Falha de Prótese , Stents , Implante de Prótese Vascular/efeitos adversos , Procedimentos Endovasculares/efeitos adversos , Análise de Falha de Equipamento , Europa (Continente) , Humanos , Dados Preliminares , Avaliação de Programas e Projetos de Saúde , Desenho de Prótese , Fatores de Risco , Propriedades de Superfície , Fatores de Tempo , Resultado do TratamentoRESUMO
INTRODUCTION: Rupture is the worst outcome of abdominal aortic aneurysm (AAA). The decision to operate should include counterbalancing the risk of aneurysm rupture against the risk of aneurysm repair, within the context of a patient's overall life expectancy. Current surgical guidelines are based on population studies, and important variables are missed in predicting individual risk of rupture. METHODS: In this literature review, we focused on the contribution of biomechanical and mathematical models in predicting risk of AAA rupture. RESULTS: Anatomical features as diameter asymmetry and lack of tortuosity are shown to be anatomical risk factors of rupture. Wall stiffness (due to modifications of elastin and collagen composition) and increased inflammatory response are also factors that affect the structural integrity of the AAA wall. Biomechanical studies showed that wall strength is lower in ruptured than non-ruptured AAA. Intra-luminal thrombus also has a big role to play in the occurrence of rupture. Current mathematical models allow more variables to be included in predicting individual risk of rupture. CONCLUSION: Moving away from using maximal transverse diameter of the AAA as a unique predictive factor and instead including biological, structural and biomechanical variables in predicting individual risk of rupture will be essential in the future and will help gain precision and accuracy in surgical indications.
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Aneurisma da Aorta Abdominal/complicações , Ruptura Aórtica/etiologia , Modelos Cardiovasculares , Estresse Mecânico , Antropometria , Aorta Abdominal/patologia , Aorta Abdominal/fisiopatologia , Aneurisma da Aorta Abdominal/fisiopatologia , Ruptura Aórtica/fisiopatologia , Ruptura Aórtica/prevenção & controle , Aortite/complicações , Aortite/fisiopatologia , Arteriopatias Oclusivas/complicações , Fenômenos Biomecânicos , Tomada de Decisão Clínica , Humanos , Hipertensão/complicações , Ensaios Clínicos Controlados Aleatórios como Assunto , Medição de Risco , Fatores de Risco , Ruptura Espontânea , Fumar/efeitos adversos , Trombose/complicações , Rigidez VascularRESUMO
In order to get a marketing authorization, breast implants (BI) must meet a number of standard requirements. French and European standards ISO 14607 list a number of official tests to be performed before an implant can be used clinically. However, the BI material characteristics evolution over implantation time remains a research field which is unexplored. The goal of the present study is to compare the mechanical ageing of two breast implant generations and assess if the use of one generation rather than the other is advantageous in terms of durability. For that purpose, 21 explanted BI were analyzed in terms of biomechanical characteristics and compared. Twelve BI were textured anatomic specimens of 5th generation and 10 BI were round textured specimens of 4th generation. All the specimens were produced by the same manufacturer. Implantation time ranged from 3 to 130 months. Both the shell and the gel of every specimen were analyzed. Results show that the mechanical properties go down with the implantation time for all the implants. Moreover, the shell of round implants appear to be less resistant than the shell of anatomic specimens with 25% lower rupture forces. With regard to the gel, whatever the specimen, results show that the properties change with implantation time. The color changes from transparent to milky to finally become yellow, while the cohesion goes down especially for the round specimens. Globally, the study brings out that BI get degraded with implantation time and provides information which could help predicting the durability of the implant.
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Implantes de Mama , Teste de Materiais , Fenômenos Mecânicos , Módulo de Elasticidade , Géis , Estresse Mecânico , Fatores de TempoRESUMO
In the last decades, main evolutions in the field of vascular surgery have been correlated to the development of devices allowing more reliable and safe sustainable treatment. First devices that have been proposed were vascular prostheses made of polymeric materials. The second generation of devices was stents made of metals and alloys. The third generation, endografts, associated these both materials. Materials used as vascular and endovascular devices must meet a number of requirements based on dimensional, physical and mechanical criteria. Ideally, they should demonstrate a behavior as close as possible as that of human arteries in terms of mechanical properties such as compliance, long-term durability, and in terms of biological properties such as biocompatibility, luminal surface healing and thrombogenicity. We propose in the present manuscript a review of properties of materials currently used for the construction of vascular and endovascular devices, future challenges in the fields of new materials and scientific approaches and tests to understand and predict the behavior of the next generations of devices.
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Materiais Biocompatíveis , Implante de Prótese Vascular/instrumentação , Prótese Vascular , Procedimentos Endovasculares/instrumentação , Stents , Implante de Prótese Vascular/efeitos adversos , Complacência (Medida de Distensibilidade) , Procedimentos Endovasculares/efeitos adversos , Humanos , Metais/química , Polímeros/química , Desenho de Prótese , Falha de Prótese , Estresse Mecânico , Resultado do TratamentoRESUMO
Endovascular treatment and stent implantation for peripheral arterial disease have been proposed for over 20 years. However, the first experiments with stainless stents were relatively disappointing. The first improvement consisted in the introduction of nitinol self-expanding stents. This technology allowed an initial improvement of clinical performances, but the first generation of nitinol stents demonstrated a relatively high rate of fractures. Better knowledge of arterial biomechanics and advances in technology allowed to propose a second generation of nitinol stents with improved flexibility, which decreased the rates of fracture. In-stent restenosis related to neointimal hyperplasia has also led to the development of new concepts to improve patency rates after stenting: drug-eluting stents (coated-stents), biodegradable stents, and covered stents. These technologies will help to treat more complex lesions in the future, but we are still waiting for results of ongoing studies.
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Prótese Vascular/tendências , Doença Arterial Periférica/cirurgia , Stents/tendências , Humanos , Desenho de PróteseRESUMO
Percutaneous aortic valve implantation has become an alternative technique to surgical valve replacement in patients with high risk for surgery. This technique is at its beginning and stents used for valve prostheses remain standard vascular stents. These stents are, however, not designed to undergo heart valve stress. They do not match the aortic environment geometry, and induce exaggerated tissue traumatism. Reduced implant lifetime may therefore be expected. The purpose of the present work is to evaluate in vitro the technical feasibility of noninvasive aortic valve replacement with a novel more specific stent. This stent is especially adapted to its implantation environment with a design that matches the shape of the aortic root while respecting the valve functions. We present a design, a manufacturing process and in vitro performances for the stent under static pressure loading and pulsatile flow. The stent shows good dynamic behavior in keeping position imposed at implantation time and in matching the aortic root dimensions changes. Prosthesis static and dynamic regurgitation are evaluated and show values close to those obtained with other commercially available prostheses.
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Próteses Valvulares Cardíacas , Stents , Valva Aórtica/cirurgia , Vias de Administração de Medicamentos , Valvas Cardíacas/cirurgia , Humanos , Próteses e Implantes , Procedimentos Cirúrgicos TorácicosRESUMO
The full-length nucleotide sequence of the genomic RNA of a new cytorhabdovirus infecting lettuce was determined. Six open reading frames were found in the antigenomic sequence of the 12,926-nt negative-sense viral RNA genome. The genomic organisation was similar to that of lettuce necrotic yellows virus (LNYV), the type member of the genus Cytorhabdovirus: 3'-N-P-3-M-G-L-5', where N is the capsid protein gene, P the putative phosphoprotein gene, 3 a gene coding for a putative protein of unknown function, M the putative matrix protein gene, G the glycoprotein gene, and L the putative polymerase gene. Amino acid sequence comparison with the corresponding sequences of other rhabdoviruses revealed the closest relationship to LNYV, with identities ranging from 41% for the matrix proteins and 65% for the L polymerase proteins. These results indicate that this virus may be a member of a new cytorhabdovirus species, for which the name Lettuce yellow mottle virus (LYMoV) is proposed.
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Genoma Viral , Lactuca/virologia , Vírus de Plantas/genética , Rhabdoviridae/classificação , Rhabdoviridae/genética , Sequência de Bases , Genes Virais , Dados de Sequência Molecular , Vírus do Mosaico/genética , RNA Viral/análise , RNA Viral/genética , Rhabdoviridae/metabolismo , Análise de Sequência de DNA , Proteínas da Matriz Viral/genética , Proteínas Virais/genéticaRESUMO
Higher plants employ a homology-dependent RNA-degradation system known as posttranscriptional gene silencing (PTGS) as a defense against virus infection. Several plant viruses are known to encode proteins that can suppress PTGS. Here we show that P0 of beet western yellows virus (BWYV) displays strong silencing suppressor activity in a transient expression assay based upon its ability to inhibit PTGS of green fluorescent protein (GFP) when expressed in agro-infiltrated leaves of Nicotiana benthamiana containing a GFP transgene. PTGS suppressor activity was also observed for the P0s of two other poleroviruses, cucurbit aphid-borne yellows virus and potato leafroll virus. P0 is encoded by the 5'-proximal gene in BWYV RNA but does not accumulate to detectable levels when expressed from the genome-length RNA during infection. The low accumulation of P0 and the resulting low PTGS suppressor activity are in part a consequence of the suboptimal translation initiation context of the P0 start codon in viral RNA, although other factors, probably related to the viral replication process, also play a role. A mutation to optimize the P0 translation initiation efficiency in BWYV RNA was not stable during virus multiplication in planta. Instead, the P0 initiation codon in the progeny was frequently replaced by a less efficient initiation codon such as ACG, GTG, or ATA, indicating that there is selection against overexpression of P0 from the viral genome.
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Inativação Gênica , Luteovirus/fisiologia , Processamento Pós-Transcricional do RNA/efeitos dos fármacos , Supressão Genética , Proteínas Virais/farmacologia , Sequência de Aminoácidos , Proteínas de Fluorescência Verde , Proteínas Luminescentes/genética , Luteovirus/genética , Luteovirus/metabolismo , Dados de Sequência Molecular , Folhas de Planta/genética , Folhas de Planta/virologia , Proteínas de Plantas/metabolismo , Nicotiana/genética , Nicotiana/virologia , Transgenes , Proteínas Virais/química , Proteínas Virais/genética , Proteínas Virais/metabolismoRESUMO
We initiated a prospective study with a group of practitioners to assess the etiology, clinical presentation, and outcome of community-acquired pneumonia in patients diagnosed in the outpatient setting. All patients with signs and symptoms suggestive of pneumonia and an infiltrate on chest X-ray underwent an extensive standard workup and were followed over 4 weeks. Over a 4-year period, 184 patients were eligible, of whom 170 (age range, 15-96 yr; median, 43 yr) were included and analyzed. In 78 (46%), no etiologic agent could be demonstrated. In the remaining 92 patients, 107 etiologic agents were implicated: 43 were due to "pyogenic" bacteria (39 Streptococcus pneumoniae, 3 Haemophilus spp., 1 Streptococcus spp.), 39 were due to "atypical" bacteria (24 Mycoplasma pneumoniae, 9 Chlamydia pneumoniae, 4 Coxiella burnetii, 2 Legionella spp.), and 25 were due to viruses (20 influenza viruses and 5 other respiratory viruses). There were only a few statistically significant clinical differences between the different etiologic categories (higher age and comorbidities in viral or in episodes of undetermined etiology, higher neutrophil counts in "pyogenic" episodes, more frequent bilateral and interstitial infiltrates in viral episodes). There were 2 deaths, both in patients with advanced age (83 and 86 years old), and several comorbidities. Only 14 patients (8.2%) required hospitalization. In 6 patients (3.4%), the pneumonia episode uncovered a local neoplasia. This study shows that most cases of community-acquired pneumonia have a favorable outcome and can be successfully managed in an outpatient setting. Moreover, in the absence of rapid and reliable clinical or laboratory tests to establish a definite etiologic diagnosis at presentation, the spectrum of the etiologic agents suggest that initial antibiotic therapy should cover both S. pneumoniae and atypical bacteria, as well as possible influenza viruses during the epidemic season.
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Pneumonia/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Análise de Variância , Infecções Comunitárias Adquiridas , Comorbidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pneumonia/diagnóstico , Pneumonia/etiologia , Pneumonia/fisiopatologia , Pneumonia/terapia , Estudos Prospectivos , Estações do Ano , Sensibilidade e Especificidade , Suíça/epidemiologia , Resultado do TratamentoRESUMO
BACKGROUND: This multicentre study compared a 16-week buspirone treatment with placebo in patients presenting with panic disorder with agoraphobia and also receiving cognitive behaviour therapy (CBT). METHOD: Double-blind testing was maintained until week 68, but not tested; 91 patients were included; 14 placebo-responders excluded; 77 patients randomised; 48 reached week 16 and 41 reached week 68. RESULTS: At week 16, within-group analysis showed significant improvements in agoraphobia, panic attacks, and depression in both groups. Generalised anxiety improved only in CBT+buspirone. Between-group comparisons showed buspirone to have an effect on generalised anxiety and agoraphobia. Changes in degree of agoraphobia and depression were correlated in subjects on CBT+buspirone only. A significantly higher proportion of women, and of subjects showing high avoidance dropped out. Positive expectations regarding medication predicted success in both groups. At week 68, improvement was retained without significant buspirone effect. CONCLUSION: Buspirone enhanced the effects of cognitive behaviour therapy on generalised anxiety and agoraphobia in the short term.
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Agorafobia/terapia , Ansiolíticos/uso terapêutico , Buspirona/uso terapêutico , Terapia Cognitivo-Comportamental , Transtorno de Pânico/terapia , Adolescente , Adulto , Agorafobia/psicologia , Ansiolíticos/efeitos adversos , Buspirona/efeitos adversos , Transtorno Depressivo/psicologia , Transtorno Depressivo/terapia , Relação Dose-Resposta a Droga , Método Duplo-Cego , Esquema de Medicação , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Transtorno de Pânico/psicologia , Resultado do TratamentoRESUMO
A multicentre double-blind, placebo-controlled clinical trial, involving 123 patients at 10 centres, was carried out to assess the efficacy of a preparation of lactic acid-producing Enterococcus SF 68 in the prevention of antibiotic-associated diarrhoea and in the treatment of acute diarrhoea. In the prevention study, 45 patients being treated with antibiotics were given, concurrently, one capsule twice daily of either Enterococcus SF68 or placebo. Acute diarrhoea was present in 78 patients who were given the same treatment but three times daily. All treatments were continued for 7 days. Enterococcus SF 68 was shown to be effective in reducing the incidence of antibiotic-associated diarrhoea in comparison with placebo (8.7% compared with 27.2%, respectively). Patients with acute enteritis showed a significantly faster resolution of bowel abnormalities during treatment with Enterococcus SF68 compared with placebo.
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Antibacterianos/efeitos adversos , Vacinas Bacterianas/uso terapêutico , Diarreia/prevenção & controle , Streptococcus/imunologia , Adulto , Ensaios Clínicos como Assunto , Diarreia/etiologia , Método Duplo-Cego , Feminino , Humanos , Masculino , Infecções Respiratórias/tratamento farmacológico , Infecções Urinárias/tratamento farmacológicoRESUMO
The study investigates clinical nutrition of oncological patients as an essential adjuvant component in the whole therapeutical concept. The main subject of the study was the transfer of nutritional concepts which were developed in non-malignant diseases to oncological patients. We defined the homeostasis of patients by biochemical and biophysical parameters in blood (osmolality, Na, K, total protein, albumin, triglycerides, NEFA, glucose, lactate, creatinine, urea, total nitrogen, amino acids) and urine (volume, osmolality, Na, K, creatinine, urea, total nitrogen, and amino acids). Of special interest was the homeostasis of nitrogen, which was characterized by the loss of nitrogen and nitrogen balances. 10 patients with either transplantable panmyeolopathy or leukemia were investigated including a phase of immunsupressive treatment by total body radiation and cytostatic treatment. Parenteral nutrition was made with amino acids (1 g/kg/d), carbohydrates (6.5 g/kg/d) and fat (1 g/kg/d). During the preparatory phase nutrition was interrupted for two consecutive days because high amounts of electrolyte solution with up to 6 1/day were needed to protect the kidney. The period of investigation covered the complete period of treatment which endured up to three months. The essential result was the achievement of a constant body weight which decreased drastically under the conventional treatment without optimized nutrition. The homeostasis remained unchanged inspite of the fact that great changes occurred individually. Nitrogen balance and nitrogen loss demonstrate the strong influence of immun-suppresive treatment with N-losses of up to 20 g per day.(ABSTRACT TRUNCATED AT 250 WORDS)