RESUMO
The calcium channel CACNA1A gene encodes the pore-forming, voltage-sensitive subunit of the voltage-dependent calcium Ca(v)2.1 type channel. Mutations in this gene have been linked to several human disorders, including familial hemiplegic migraine, episodic ataxia 2 and spinocerebellar ataxia type 6. The mouse homologue, Cacna1a, is associated with the tottering, Cacna1a(tg), mutant series. Here we describe two new missense mutant alleles, Cacna1a(tg-4J) and Cacna1a(Tg-5J). The Cacna1a(tg-4J) mutation is a valine to alanine mutation at amino acid 581, in segment S5 of domain II. The recessive Cacna1a(tg-4J) mutant exhibited the ataxia, paroxysmal dyskinesia and absence seizures reminiscent of the original tottering mouse. The Cacna1a(tg-4J) mutant also showed altered activation and inactivation kinetics of the Ca(v)2.1 channel, not previously reported for other tottering alleles. The semi-dominant Cacna1a(Tg-5J) mutation changed a conserved arginine residue to glutamine at amino acid 1252 within segment S4 of domain III. The heterozygous mouse was ataxic and homozygotes rarely survived. The Cacna1a(Tg-5J) mutation caused a shift in both voltage activation and inactivation to lower voltages, showing that this arginine residue is critical for sensing Ca(v)2.1 voltage changes. These two tottering mouse models illustrate how novel allelic variants can contribute to functional studies of the Ca(v)2.1 calcium channel.
Assuntos
Canais de Cálcio Tipo N/genética , Mutação , Doenças do Sistema Nervoso , Alanina/genética , Animais , Animais Recém-Nascidos , Calbindinas , Células Cultivadas , Cisteína/genética , Modelos Animais de Doenças , Glicina/genética , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Ativação do Canal Iônico/genética , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/genética , Potenciais da Membrana/efeitos da radiação , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Mutantes , Modelos Moleculares , Doenças do Sistema Nervoso/genética , Doenças do Sistema Nervoso/patologia , Doenças do Sistema Nervoso/fisiopatologia , Técnicas de Patch-Clamp , Células de Purkinje/patologia , Células de Purkinje/fisiologia , Células de Purkinje/ultraestrutura , Proteína G de Ligação ao Cálcio S100/metabolismo , Coloração pela Prata/métodos , Treonina/genética , Tirosina 3-Mono-Oxigenase/metabolismoRESUMO
Mild-to-moderate AD patients were randomized to placebo or rosiglitazone (RSG) 2, 4 or 8 mg. Primary end points at Week 24 were mean change from baseline in AD Assessment Scale-Cognitive (ADAS-Cog) and Clinician's Interview-Based Impression of Change Plus Caregiver Input global scores in the intention-to-treat population (N=511), and results were also stratified by apolipoprotein E (APOE) genotype (n=323). No statistically significant differences on primary end points were detected between placebo and any RSG dose. There was a significant interaction between APOE epsilon4 allele status and ADAS-Cog (P=0.014). Exploratory analyses demonstrated significant improvement in ADAS-Cog in APOE epsilon4-negative patients on 8 mg RSG (P=0.024; not corrected for multiplicity). APOE epsilon4-positive patients did not show improvement and showed a decline at the lowest RSG dose (P=0.012; not corrected for multiplicity). Exploratory analyses suggested that APOE epsilon4 non-carriers exhibited cognitive and functional improvement in response to RSG, whereas APOE epsilon4 allele carriers showed no improvement and some decline was noted. These preliminary findings require confirmation in appropriate clinical studies.
Assuntos
Doença de Alzheimer/tratamento farmacológico , Apolipoproteínas E/genética , Hipoglicemiantes/uso terapêutico , Tiazolidinedionas/uso terapêutico , Idoso , Alelos , Doença de Alzheimer/genética , Apolipoproteína E4 , Apolipoproteínas E/deficiência , Cognição/efeitos dos fármacos , Relação Dose-Resposta a Droga , Feminino , Genótipo , Humanos , Hipoglicemiantes/efeitos adversos , Masculino , Farmacogenética , Rosiglitazona , Tiazolidinedionas/efeitos adversosRESUMO
We have identified a migraine locus on chromosome 19p13.3/2 using linkage and association analysis. We isolated 48 single-nucleotide polymorphisms within the locus, of which we genotyped 24 in a Caucasian population comprising 827 unrelated cases and 765 controls. Five single-nucleotide polymorphisms within the insulin receptor gene showed significant association with migraine. This association was independently replicated in a case-control population collected separately. We used experiments with insulin receptor RNA and protein to investigate functionality for the migraine-associated single-nucleotide polymorphisms. We suggest possible functions for the insulin receptor in migraine pathogenesis.
Assuntos
Alelos , Transtornos de Enxaqueca/genética , Polimorfismo de Nucleotídeo Único , Receptor de Insulina/genética , Sequência de Bases , Estudos de Casos e Controles , Mapeamento Cromossômico , Cromossomos Artificiais Bacterianos , Cromossomos Humanos Par 19 , Primers do DNA , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Desequilíbrio de Ligação , Masculino , Ligação Proteica , Receptor de Insulina/metabolismo , Reprodutibilidade dos Testes , População Branca/genéticaAssuntos
Canais de Cálcio/metabolismo , Ataxia Cerebelar/fisiopatologia , Cerebelo/patologia , Marcação de Genes , Animais , Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio/genética , Canais de Cálcio Tipo N , Canais de Cálcio Tipo P , Canais de Cálcio Tipo Q , Células Cultivadas , Ataxia Cerebelar/genética , Cerebelo/citologia , Cerebelo/efeitos dos fármacos , Cerebelo/fisiopatologia , Humanos , Camundongos , Camundongos Transgênicos , Nimodipina/farmacologia , Técnicas de Patch-Clamp , ômega-Conotoxina GVIA/farmacologia , ômega-Conotoxinas/farmacologiaRESUMO
To understand the cellular and molecular mechanisms that underlie generalized absence seizures sufficiently well to design rational, efficacious new therapies for patients, it is necessary to turn to animal models to gain insights into these mechanisms. The lethargic (lh/lh) mutant mouse expresses spontaneous absence seizures that share behavioral, electrographic, and anticonvulsant profiles with absence seizures in patients. This validates its use to study the mechanisms that underlie absence seizures. This chapter discusses two scientific approaches that involve the use of lh/lh mice. The first part of the chapter discusses neurobiologic approaches used to investigate critical mechanisms that regulate the synchronized burst firing within the thalamocortical network that generates absence seizures. Two of these critical mechanisms have been studied in detail with lh/lh mice. The first critical mechanism involves the required activation of gamma-aminobutyric acid B (GABAB) receptors to generate absence seizures. Because the numbers of GABAB receptors are increased in thalamocortical populations among lh/lh mice compared with littermates without epilepsy, these receptors appear to play a pathophysiologic role in the expression of absence seizures among lh/lh mice. Moreover, there may be a role for GABAB receptors in the generation of absence seizures among humans, because administration of compounds that activate GABAB receptors can produce absence seizures among humans. These findings suggest that GABAB receptor antagonists may represent a new class of antiabsence compounds that will be efficacious against absence seizures among patients. A second critical mechanism that regulates generation of absence seizures involves GABAA receptors in the nucleus reticularis thalami (NRT), a nucleus that sends GABA-ergic afferents to thalamic relay nuclei. Activation of GABAA receptors in the NRT appears to suppress the generation of absence seizures among lh/lh mice and in other models. Moreover, clonazepam may exert its antiabsence actions through this mechanism. Together, these findings suggest that compounds that selectively activate GABAA receptor isoforms expressed in NRT may represent a class of antiabsence drugs that could have fewer side effects than compounds currently used to treat patients. The second part of the chapter discusses a molecular genetic approach to delineation of the mechanisms that underlie absence seizures. Absence seizures among lh/lh mice are caused by a single-gene defect on chromosome 2. If positional cloning and gene isolation techniques are successful, it will be possible to identify the lh disease gene. Subsequent studies of the lh gene product should greatly increase not only our understanding of the pathophysiologic basis for absence seizures among lh/lh mice but also our ability to seek similar mutations in homologous genes in human families that express absence seizures. Accordingly, strategies and progress in cloning and identifying the lh disease gene are presented.
Assuntos
Clonagem Molecular , Epilepsia Tipo Ausência/genética , Regulação da Expressão Gênica , Camundongos Mutantes Neurológicos/genética , Camundongos Mutantes Neurológicos/fisiologia , Receptores de GABA/genética , Animais , Mapeamento Cromossômico , CamundongosRESUMO
Nonconvulsive status epilepticus includes three clinical situations: complex partial status epilepticus; absence status epilepticus: and obtundation in the presence of electrographic status epilepticus. Animal models that provide information helpful to clinical management exist for both complex partial and absence status epilepticus. In models of complex partial status epilepticus (pilocarpine, kainic acid, and various protocols using electrical stimulation), neuronal damage in discrete neuronal populations follows an episode of status epilepticus. Hippocampal populations are particularly susceptible to neuropathologic sequelae. Although it is difficult in some cases to distinguish whether the inducing agent or the status epilepticus causes neuropathology, the similar patterns of damage caused by different inducing stimuli provide converging lines of evidence suggesting that the neuropathologic consequences stem at least in part from status epilepticus. In models of absence status epilepticus (genetic mutants, pentylenetetrazole), there is relatively scarce neuropathology that can be attributed directly to status epilepticus. Together these data from animal models suggest that neuropathologic consequences from complex partial status epilepticus may be more severe than those from absence status epilepticus. If these findings translate to patients, then nonconvulsive status epilepticus of the complex partial type should be managed more aggressively than nonconvulsive status epilepticus of the absence type.
Assuntos
Estado Epiléptico/fisiopatologia , Animais , Encéfalo/patologia , Modelos Animais de Doenças , Epilepsias Parciais/patologia , Epilepsias Parciais/fisiopatologia , Epilepsia Tipo Ausência/patologia , Epilepsia Tipo Ausência/fisiopatologia , Humanos , Estado Epiléptico/patologiaRESUMO
The mutated gene in the lethargic (Cacnb4lh) mouse model of absence seizures encodes the beta4 subunit of voltage-gated calcium channels (VGCCs), leading to decreased mRNA expression of a beta4 subunit that is truncated and cannot bind to alpha1 subunits of VGCCs. In this study we accomplished two goals. First, we studied the functional consequence of altered VGCCs by examining the effects of a selective P/Q-type channel antagonist on KCl-induced (45)Ca(2)(+) uptake in brain synaptosomes from Cacnb4lh homozygotes and non-epileptic controls (designated by +/+). We found that depolarization-induced (45)Ca(2)(+) uptake was significantly reduced in the brains of Cacnb4lh homozygotes, and that the reduced uptake was completely accounted for by reduced function of P/Q-type calcium channel. Second, we examined VGCC subunit composition to determine if other subunits were altered in addition to the mutation affecting beta4 subunits in Cacnb4lh homozygotes; when alterations were found, we determined if they were regional or global. We used in situ hybridization histochemistry (ISHH) to analyze the neuro-anatomic distribution of beta4, beta1b, beta2, beta3, alpha1A, alpha1B, alpha1C, alpha1E, and alpha1G subunit mRNAs in brain sections from matched Cacnb4lh homozygotes and +/+ controls. Our results indicated that expression of beta4 subunit mRNA is globally reduced throughout the brains of Cacnb4lh homozygotes, in contrast to a small but significant global increase in the expression of beta3 subunit mRNA. There were no significant differences in expression of the other VGCC subunit mRNAs examined. Together, these findings indicate that a host of changes in VGCC subunit composition accompany reduced function of P/Q-type channels in homozygous lethargic mice.
Assuntos
Canais de Cálcio/genética , Canais de Cálcio/metabolismo , Cálcio/metabolismo , Córtex Cerebral/metabolismo , Epilepsia/genética , Sinaptossomos/metabolismo , Transcrição Gênica , Animais , Canais de Cálcio/fisiologia , Radioisótopos de Cálcio , Epilepsia/fisiopatologia , Homozigoto , Hibridização In Situ , Substâncias Macromoleculares , Camundongos , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Camundongos Mutantes Neurológicos , Cloreto de Potássio/farmacologia , RNA Mensageiro/genéticaRESUMO
To test the hypothesis that altered GABA synthesis within nucleus reticularis thalami (NRT) neurons regulates absence seizures, we analyzed and quantitated the distribution of GAD(67) and GAD(65), the rate-limiting enzymes of GABA synthesis, in thalamic nuclei from the Cacnb4lh model of absence seizures and non-epileptic (+/+) controls. In situ hybridization and Western blot results indicate a significant increase in GAD(67) expression (mRNA and protein) per cell but no change in GAD(65) in Cacnb4lh mice. These data suggest that GABA-synthesis is maintained or increased in NRT neurons in the Cacnb4lh mouse model.
Assuntos
Epilepsia Tipo Ausência/enzimologia , Epilepsia Tipo Ausência/genética , Glutamato Descarboxilase/genética , Glutamato Descarboxilase/metabolismo , Núcleos Talâmicos/metabolismo , Animais , Canais de Cálcio/genética , Modelos Animais de Doenças , Hibridização In Situ , Isoenzimas/genética , Isoenzimas/metabolismo , Cinética , Camundongos , Camundongos Mutantes NeurológicosRESUMO
In previous studies we found increased GABA(B) receptor number in 8-week-old homozygous Cacnb4lh mice compared to nonepileptic (+/+) littermates. In this study, we examined the relationship between Cacnb4 and GABA(B) receptor mRNA expression in brains from Cacnb4lh homozygotes and (+/+) controls. We found a significant correlation between the magnitude of increased GABA(B) receptor and decreased Cacnb4 mRNA expression in 8-week-old mice. In contract, in 6-month-old mice, there was no change in GABA(B) receptor or Cacnb4 mRNA expression. These findings suggest that the factor(s) responsible for decreased Cacnb4 and increased GABA(B) receptor mRNA expression abate in older mice.
Assuntos
Envelhecimento/metabolismo , Encéfalo/metabolismo , Canais de Cálcio/genética , Regulação da Expressão Gênica no Desenvolvimento , Receptores de GABA-B/genética , Transcrição Gênica , Animais , Encéfalo/crescimento & desenvolvimento , Modelos Animais de Doenças , Epilepsia Tipo Ausência/genética , Epilepsia Tipo Ausência/fisiopatologia , Homozigoto , Camundongos , Camundongos Mutantes Neurológicos , Especificidade de Órgãos , RNA Mensageiro/genéticaRESUMO
Excitatory but not inhibitory synaptic transmission is reduced in lethargic (Cacnb4(lh)) and tottering (Cacna1atg) mouse thalami. Recent studies of the homozygous tottering (Cacna1atg) and lethargic mouse (Cacnb4(lh)) models of absence seizures have identified mutations in the genes encoding the alpha1A and beta4 subunits, respectively, of voltage-gated Ca2+ channels (VGCCs). beta subunits normally regulate Ca2+ currents via a direct interaction with alpha1 (pore-forming) subunits of VGCCs, and VGCCs are known to play a significant role in controlling the release of transmitter from presynaptic nerve terminals in the CNS. Because the gene mutation in Cacnb4(lh) homozygotes results in loss of the beta4 subunit's binding site for alpha1 subunits, we hypothesized that synaptic transmission would be altered in the CNS of Cacnb4(lh) homozygotes. We tested this hypothesis by using whole cell recordings of single cells in an in vitro slice preparation to investigate synaptic transmission in one of the critical neuronal populations that generate seizure activity in this strain, the somatosensory thalamus. The primary finding reported here is the observation of a significant decrease in glutamatergic synaptic transmission mediated by both N-methyl-D-aspartate (NMDA) and non-NMDA receptors in somatosensory thalamic neurons of Cacnb4(lh) homozygotes compared with matched, nonepileptic mice. In contrast, there was no significant decrease in GABAergic transmission in Cacnb4(lh) homozygotes nor was there any difference in effects mediated by presynaptic GABAB receptors. We found a similar decrease in glutamatergic but not GABAergic responses in Cacna1atg homozygotes, suggesting that the independent mutations in the two strains each affected P/Q channel function by causing defective neurotransmitter release specific to glutamatergic synapses in the somatosensory thalamus. This may be an important factor underlying the generation of seizures in these models.
Assuntos
Canais de Cálcio/genética , Mutação/fisiologia , Inibição Neural/fisiologia , Transmissão Sináptica/fisiologia , Tálamo/fisiologia , Animais , Canais de Cálcio Tipo N , Canais de Cálcio Tipo P , Canais de Cálcio Tipo Q , Epilepsia Tipo Ausência/genética , Epilepsia Tipo Ausência/fisiopatologia , Potenciais Pós-Sinápticos Excitadores/fisiologia , Ácido Glutâmico/fisiologia , Homozigoto , Técnicas In Vitro , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Mutantes Neurológicos/fisiologia , N-Metilaspartato/fisiologia , Ácido gama-Aminobutírico/fisiologiaRESUMO
We report a case of relapsing polychondritis with focal sensorimotor seizures, aseptic meningitis, and migratory leptomeningeal enhancement on contrast MRI. These abnormalities on imaging studies correlated accurately with laterality of the patient's seizures, facilitating early aggressive management of his neurologic symptoms.
Assuntos
Epilepsias Parciais/diagnóstico , Meningite/diagnóstico , Policondrite Recidivante/diagnóstico , Idoso , Epilepsias Parciais/complicações , Lateralidade Funcional , Humanos , Aumento da Imagem , Imageamento por Ressonância Magnética , Masculino , Meningite/complicações , Policondrite Recidivante/complicaçõesRESUMO
PURPOSE: Traditional methods of preclinical screening have predicted the effects of a putative antiepileptic drug (AED) against human absence seizures by testing its efficacy against clonic seizures in the high-dose pentylenetetrazole (PTZ) model. This high-dose PTZ model correctly predicted the efficacy of ethosuximide (ESM), benzodiazepines, and valproate (VPA) and the lack of efficacy of phenytoin (PHT) and carbamazepine (CBZ). However, the high-dose PTZ model erred in predictions for (a) phenobarbital (PB) (PTZ: efficacy; human: nonefficacy); (b) lamotrigine (LTG) (PTZ: nonefficacy; human: efficacy); (c) vigabatrin (VGB) (PTZ: nonefficacy; human: proabsence effect); and (d) tiagabine (TGB) (PTZ: efficacy; human: possible proabsence). It also appears to have erred in predictions for gabapentin (GBP) (PTZ: efficacy) and topiramate (TPM) (PTZ: efficacy). Because the lh/lh genetic model of absence seizures correctly predicted effects of ESM, clonazepam, VPA, PHT, CBZ, and PB against human absence seizures, we performed this study to test the predictive utility of the lh/lh model for LTG, VGB, TGB, GBP, and TPM. METHODS: Bipolar recording electrodes were implanted bilaterally into frontal neocortex of 8-week-old male lh/lh mice. With the exception of VGB, vehicle or drugs were administered intraperitoneally (i.p.) on alternating days, and an EEG was used to record effects on seizure frequency. With VGB, vehicle was administered i.p. on day 1, and gradually increasing doses of VGB were administered on successive days. Drug and vehicle effects were compared in corresponding 15-min epochs of the 150-min period after administration. RESULTS: LTG (4.8-144 micromol/kg) significantly (p < 0.04) reduced seizure frequency (by 65%) compared with vehicle. In contrast, VGB (0.35-11 mmol/kg) and TGB (0.27-27 micromol/kg) significantly increased seizure frequency (300-700%) and seizure duration (1,700-1,800%; p < 0.001). GBP (18 micromol/kg to 1.8 mmol/kg) and TPM (8.9-295 micromol/kg) had no significant effect on seizure frequency. CONCLUSIONS: In contrast to the high-dose PTZ model, the lh/lh model correctly predicted the antiabsence effect of LTG, the possible proabsence effects of VGB and TGB, and the lack of effect of GBP and TPM. The lh/lh model appears to be superior to the high-dose PTZ model in predicting efficacy of putative AEDs against human absence seizures.
Assuntos
Aminas , Anticonvulsivantes/uso terapêutico , Ácidos Cicloexanocarboxílicos , Epilepsia Tipo Ausência/tratamento farmacológico , Acetatos/farmacologia , Acetatos/uso terapêutico , Animais , Anticonvulsivantes/farmacologia , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Eletroencefalografia/efeitos dos fármacos , Epilepsia Tipo Ausência/genética , Frutose/análogos & derivados , Frutose/farmacologia , Frutose/uso terapêutico , Gabapentina , Humanos , Lamotrigina , Masculino , Camundongos , Ácidos Nipecóticos/farmacologia , Ácidos Nipecóticos/uso terapêutico , Tiagabina , Topiramato , Triazinas/farmacologia , Triazinas/uso terapêutico , Vigabatrina , Ácido gama-Aminobutírico/análogos & derivados , Ácido gama-Aminobutírico/farmacologia , Ácido gama-Aminobutírico/uso terapêuticoRESUMO
Absence seizures represent synchronized burst-firing of thalamocortical neurons, which are driven by tonic GABAergic output of nucleus reticularis thalami (NRT). Activation of GABAA receptors on NRT neurons reduces NRT output and retards thalamocortical burst-firing. Although this mechanism in NRT may underlie antiabsence effects of benzodiazepines, it does not explain observations that barbiturates can worsen absence-seizures. In this study we tested the hypothesis that clonazepam and phenobarbital produce differential effects on GABAA receptors in the lh/lh genetic model of absence seizures after microinjection into NRT compared to VLa, a prototypic relay nucleus containing thalamocortical neurons. In NRT, phenobarbital (16-1600 nmol/cannula), clonazepam (160-2200 pmol/cannula) and muscimol (8.8-263 pmol/cannula) significantly suppressed absence seizure frequency. In VLa, phenobarbital (1.6 nmol) and muscimol (0.88 pmol) increased seizure frequency, whereas higher doses (160 nmol and 88 pmol, respectively) significantly suppressed seizure frequency. In contrast, clonazepam produced no effect on seizure frequency even at a dose of 2.2 nmol; this same dose significantly suppressed absence seizures after microinjection into NRT. These findings suggest that activation of GABAA receptors in NRT may suppress absence seizures, and that phenobarbital may worsen absence seizures through actions on GABAA receptors in thalamocortical cells (VLa). Region-specific GABAA receptor isoforms may underlie the contrasting effects of clonazepam after microinjection into NRT and VLa.
Assuntos
Epilepsia Tipo Ausência/tratamento farmacológico , Agonistas GABAérgicos/farmacologia , Moduladores GABAérgicos/farmacologia , Receptores de GABA-A/efeitos dos fármacos , Núcleos Talâmicos/fisiopatologia , Animais , Clonazepam/administração & dosagem , Clonazepam/farmacologia , Relação Dose-Resposta a Droga , Eletroencefalografia/efeitos dos fármacos , Epilepsia Tipo Ausência/genética , Epilepsia Tipo Ausência/fisiopatologia , Feminino , Agonistas GABAérgicos/administração & dosagem , Moduladores GABAérgicos/administração & dosagem , Agonistas de Receptores de GABA-A , Masculino , Camundongos , Camundongos Endogâmicos , Microinjeções , Muscimol/administração & dosagem , Muscimol/farmacologia , Fenobarbital/administração & dosagem , Fenobarbital/farmacologia , Núcleos Talâmicos/anatomia & histologiaRESUMO
In the past year we have gained significant insights into the molecular and genetic mechanisms underlying generalized absence seizures, primarily through the study of animal models. Also a new understanding has emerged about the genetic bases of certain syndromes in which generalized myoclonic and tonic-clonic seizures are expressed. New insights into these different types of generalized seizures may lead eventually to new therapies.
Assuntos
Epilepsia Generalizada/genética , Animais , Encéfalo/anormalidades , Encéfalo/fisiopatologia , Epilepsias Mioclônicas/genética , Epilepsias Mioclônicas/fisiopatologia , Epilepsia Tipo Ausência/genética , Epilepsia Tipo Ausência/fisiopatologia , Epilepsia Generalizada/fisiopatologia , Epilepsia Tônico-Clônica/genética , Epilepsia Tônico-Clônica/fisiopatologia , Expressão Gênica/fisiologia , Humanos , Camundongos , Biologia Molecular , Ratos , SíndromeRESUMO
Generalized absence seizures in humans are a unique type of epilepsy characterized by a synchronous, bilateral 3-Hz spike and wave discharge emanating from a cortical and thalamic network within the brain. The availability of a number of pharmacological and genetic animal models has provided us with the means with which to investigate the cellular and molecular mechanisms underlying these seizures. Over the last few years a significant amount of research in these models has focused on the role of the inhibitory GABAB receptors, which have been previously described in a number of brain areas as being responsible for a long-lasting hyperpolarization and depression in neurotransmitter release. Initial studies provided evidence that the GABAB receptor was capable of generating the low threshold calcium spike required for initiation of the burst firing, leading researchers to hypothesize that the GABAB receptors played a significant role in these seizures. Subsequent research took advantage of the new generation of GABAB antagonists that became available in the early 1990s and demonstrated that in a number of models the seizures could be abolished by the administration of one of these compounds. Further biochemical, molecular, and electrophysiological experiments have been carried out to determine the exact involvement of GABAB receptors and their mechanism of action. The current evidence and interpretations of this work are presented here.
Assuntos
Epilepsia Tipo Ausência/fisiopatologia , Receptores de GABA-B/fisiologia , Ácido gama-Aminobutírico/fisiologia , Animais , Anticonvulsivantes/farmacologia , Córtex Cerebral/fisiopatologia , Agonistas GABAérgicos/farmacologia , Antagonistas GABAérgicos/farmacologia , Humanos , Camundongos , Camundongos Mutantes Neurológicos , Modelos Neurológicos , Ratos , Receptores de GABA-B/efeitos dos fármacos , Sistemas do Segundo Mensageiro , Tálamo/fisiopatologiaRESUMO
Whole-cell voltage-clamp recordings were made from thalamic ventrobasal (VB) neurons of age-matched lethargic (lh/lh) and wildtype (+/+) mice. Hyperpolarizing voltage commands (40 mV) from a holding potential of -60 mV were delivered to the cell and the resulting K+ conductance (gK+) activated by the GABAB receptor agonist baclofen was measured and compared between the two groups. VB cells from +/+ and lh/lh displayed no significant differences in resting conductance (gIN) or gK+ activated by baclofen. In addition to this, isolated, evoked GABAB-mediated currents were recorded in VB cells. There was no significant difference in peak amplitude or latency to peak noted between the two groups. These data suggest that postsynaptic GABAB receptor-mediated function is not altered in VB thalamic neurones in this model of absence seizures.
Assuntos
Baclofeno/farmacologia , Canais de Potássio/efeitos dos fármacos , Receptores de GABA-B/efeitos dos fármacos , Tálamo/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Epilepsia Tipo Ausência/fisiopatologia , Camundongos , Camundongos EndogâmicosRESUMO
The effects of serotonergic and dopaminergic drugs on free swimming behavior in adult sea lampreys (Petromyzon marinus) were investigated using video image analysis. Injections of the serotonin precursor 5-hydroxy-L-tryptophan along with the serotonin reuptake blocker clomipramine into the visceral cavity of lampreys resulted in significant increases in the cycle period of swimming, but had no significant effects on the propagation time of the swim waves down the body (normalized to cycle period), or on the degree of body curvature. Injections of the dopamine agonist apomorphine resulted in significant decreases of cycle period and body curvature with no significant effects on the normalized wave propagation time. The effects on cycle period are consistent with previous findings using serotonin and apomorphine on swimming activity in the isolated spinal cord.
Assuntos
Dopaminérgicos/farmacologia , Lampreias/fisiologia , Atividade Motora/efeitos dos fármacos , Serotoninérgicos/farmacologia , 5-Hidroxitriptofano/farmacologia , Animais , Apomorfina/farmacologia , Clomipramina/farmacologia , Agonistas de Dopamina/farmacologia , Processamento de Imagem Assistida por Computador , Inibidores Seletivos de Recaptação de Serotonina/farmacologia , NataçãoRESUMO
In previous work we have shown that GABAB receptors are required for expression of absence seizures in the lethargic (lh/lh) mouse model; that lh/lh mice have increased numbers of GABAB binding sites compared to nonepileptic littermates (designated +/+); and that the magnitude of the increased number of GABAB receptors in lh/lh mice correlated positively with the frequency of absence seizures. We performed this study to delineate the neural network in which GABAB receptors regulate absence seizures in lh/lh mice. We designed three successive screens which had to be passed by a candidate neuronal population before it could be considered a member of the neural network in which GABAB receptors regulate absence seizures. First, the neuronal populations in lh/lh mice had to have enriched GABAB binding sites compared to homologous populations in matched nonepileptic controls; baclofen-displaceable 3H-GABA binding was measured in autoradiograms for this screen. Second, the candidate populations had to generate spike-wave discharges (SWDs) during absence seizures in lh/lh mice; bipolar recording electrodes implanted into candidate neuronal structures were used in this screen. Third, the candidate populations had to demonstrate GABAB receptor-mediated regulation of absence seizures in lh/lh mice; microinjections of a GABAB agonist [(-)-baclofen] and antagonist (CGP 35348) were used for this screen. In this study we found that anterior ventral lateral thalamic nucleus (VLa), nucleus reticularis thalami (NRT), nucleus reuniens (RE) passed all three screens, and hence are members of the neural network in which GABAB receptors regulate absence seizures in lh/lh mice.
Assuntos
Epilepsia Tipo Ausência/fisiopatologia , Rede Nervosa/fisiologia , Receptores de GABA-B/fisiologia , Animais , Autorradiografia , Baclofeno/farmacologia , Eletroencefalografia , Epilepsia Tipo Ausência/genética , Antagonistas GABAérgicos/farmacologia , Agonistas dos Receptores de GABA-B , Camundongos , Camundongos Mutantes , Microinjeções , Compostos Organofosforados/farmacologia , Fases do SonoRESUMO
Previously, we have shown a significant increase in number of GABAB receptor binding sites in neocortex and thalamus of lethargic (lh/lh) mice, a mutant strain exhibiting absence seizures. This study was performed to test our hypothesis that presynaptic GABAB receptors would inhibit [3H]GABA release to a greater degree in lh/lh mice compared with their nonepileptic littermates (designated +/+). Synaptosomes isolated from neocortex and thalamus of age-matched male lh/lh and +/+ mice were similar in uptake of [3H]GABA. In the neocortical preparation, baclofen dose-dependently inhibited [3H]GABA release evoked by 12 mM KCl, an effect mediated by GABAB receptors. The maximal inhibition (Imax) value was significantly greater (80%) in lh/lh than +/+ mice, whereas the IC50 (3 microM) was unchanged. In the thalamic preparation, the effect of baclofen (50 microM) was 58% less robust in lh/lh mice. Other effects mediated by GABAB receptors (inhibitions in Ca2+ uptake and cyclic AMP formation) were also significantly reduced in thalamic synaptosomes from lh/lh mice. These data suggest a greater presynaptic GABAB receptor-mediated effect in neocortex and a reduced effect in thalamic nuclei of lh/lh mice. It is possible that selective effects of presynaptic GABAB receptors or GABA release in neocortex and thalamic nuclei of lh/lh mice may contribute to mechanisms underlying absence seizures.
Assuntos
Epilepsia Tipo Ausência/fisiopatologia , Receptores de GABA-B/fisiologia , Sinaptossomos/fisiologia , Animais , Cálcio/metabolismo , Córtex Cerebral/metabolismo , Colforsina/farmacologia , AMP Cíclico/biossíntese , Epilepsia/genética , Epilepsia/metabolismo , Epilepsia Tipo Ausência/genética , Hibridização Genética , Masculino , Camundongos , Camundongos Endogâmicos , Cloreto de Potássio/farmacologia , Tálamo/metabolismo , Ácido gama-Aminobutírico/metabolismoRESUMO
Recent studies have shown that gamma-aminobutyric acidB (GABAB) receptor antagonists suppress absence seizures in animal models. (+)-5,5-Dimethyl-2-morpholineacetic acid, hydrochloride (SCH 50911) is a new GABAB antagonist that is structurally dissimilar to previously studied GABAB antagonists such as 3-aminopropyl-diethoxymethyl-phosphinic acid (CGP 35348), 3-aminopropyl-n-butyl-phosphinic acid (CGP 36742) or 3-aminopropyl-cyclohexylmethyl-phosphinic acid (CGP 46381). In this study we measured the antiabsence effects of SCH 50911 in three animal models: the lethargic (lh/lh) mutant mouse, which has spontaneous absence seizures; and two rat models in which absence seizures were induced by administration of either gamma-hydroxybutyrate or pentylenetetrazole. SCH 50911 abolished seizures in all three models in a dose-dependent fashion (ID100 = 8-170 mumol/kg). In each model SCH 50911 was more potent (ID50 = 2-22 mumol/kg) than the following antiabsence compounds: the GABAB antagonist CGP 35348 (ID50 = 210-890 mumol/kg); ethosuximide (ID50 < or = 142-1240 mumol/kg); trimethadione (ID50 = 520-1100 mumol/kg); and valproic acid (ID50 = 900-2360 mumol/kg). SCH 50911 was equipotent with the GABAB antagonist CGP 46381 (ID50 = 20 mumol/kg) in the lh/lh mouse model. These findings suggest that antiabsence activity may be a defining feature of GABAB receptor antagonists and provide a rationale for pursuing clinical trials of GABAB receptor antagonists in human patients with absence seizures.