RESUMO
BACKGROUND: Toxoplasma gondii infection, if acquired as an acute infection during pregnancy, can have substantial adverse effects on mothers, fetuses and newborns. Latent toxoplasmosis also causes a variety of pathologies and has been linked to adverse effects on pregnancy. OBJECTIVE: Here, we present results of a comprehensive systematic review and meta-analysis of the global prevalence of latent toxoplasmosis in pregnant women. DATA SOURCE: We searched PubMed, EMBASE, Web of Science, SciELO and Scopus databases for relevant studies that were published between 1 January 1988 and 20 July 2019. STUDY ELIGIBILITY CRITERIA: All population-based, cross-sectional and longitudinal studies reporting the prevalence of latent toxoplasmosis in healthy pregnant women were considered for inclusion. PARTICIPANTS: Pregnant women who were tested for prevalence of latent toxoplasmosis. INTERVENTIONS: There were no interventions. METHOD: We used a random effects model to calculate pooled prevalence estimates with 95% confidence intervals (CIs). We grouped prevalence data according to the geographic regions defined by the World Health Organization (WHO). Multiple subgroup and meta-regression analyses were performed. RESULTS: In total, 311 studies with 320 relevant data sets representing 1 148 677 pregnant women from 91 countries were eligible for inclusion in the meta-analysis. The global prevalence of latent toxoplasmosis in pregnant women was estimated at 33.8% (95% CI, 31.8-35.9%; 345 870/1 148 677). South America had the highest pooled prevalence (56.2%; 50.5-62.8%) of latent toxoplasmosis in pregnant women, whereas the Western Pacific region had the lowest prevalence (11.8%; 8.1-16.0%). A significantly higher prevalence of latent toxoplasmosis was associated with countries with low income and low human development indices (p < 0.001). CONCLUSION: Our results indicate a high level of latent toxoplasmosis in pregnant women, especially in some low- and middle-income countries of Africa and South America, although the local prevalence varied markedly. These results suggest a need for improved prevention and control efforts to reduce the health risks to women and newborns.
Assuntos
Anticorpos Antiprotozoários/sangue , Infecção Latente/epidemiologia , Complicações Infecciosas na Gravidez/epidemiologia , Toxoplasmose/epidemiologia , Estudos Transversais , Feminino , Saúde Global , Humanos , Infecção Latente/parasitologia , Estudos Longitudinais , Gravidez , Complicações Infecciosas na Gravidez/parasitologia , Prevalência , Toxoplasma/imunologiaRESUMO
BACKGROUND: Schistosomiasis caused by Schistosoma mansoni (Sm) is a chronic, debilitating and potentially deadly neglected tropical disease. The licensure of a vaccine to prevent schistosomiasis would represent a major breakthrough in public health. METHODS: The safety and immunogenicity of a candidate Sm vaccine were assessed in this phase I, double-blind, dose-escalation trial. Seventy-two healthy Sm-naïve 18-50â¯year olds were randomized to receive 3 doses â¼â¯8â¯weeks apart of saline placebo, or 10⯵g, 30⯵g, or 100⯵g of recombinant Sm-Tetraspanin-2 vaccine formulated on aluminum hydroxide adjuvant (Sm-TSP-2/Al) with or without 5⯵g of glucopyranosyl lipid A aqueous formulation (GLA-AF). Clinical and serologic responses were assessed for 1â¯year after dose 3. RESULTS: Vaccines were safe and well-tolerated. The most common reactions were injection site tenderness and pain, and headache and fatigue. Tenderness and pain were more frequent in groups receiving vaccine with GLA-AF than placebo (pâ¯=â¯0.0036 and pâ¯=â¯0.0014, respectively). Injection site reactions among those given Sm-TSP-2/Al with GLA-AF lasted 1.22 and 1.33â¯days longer than those receiving Sm-TSP-2/Al without GLA-AF or placebo (pâ¯<â¯0.001 for both). Dose- and adjuvant-related increases in serum IgG against Sm-TSP-2 were observed. Peak IgG levels occurred 14â¯days after dose 3. Seroresponse frequencies were low among recipients of Sm-TSP-2/Al without GLA-AF, but higher among subjects receiving 30⯵g or 100⯵g of Sm-TSP-2/Al with GLA-AF. More seroresponses were observed among those given 30⯵g or 100⯵g of Sm-TSP-2/Al with GLA-AF compared to placebo (pâ¯=â¯0.023 and pâ¯<â¯0.001, respectively). Seroresponse frequencies were 0%, 30%, 50%, and 89%, respectively, among those given placebo, or 10⯵g, 30⯵g or 100⯵g of Sm-TSP-2/Al with GLA-AF, suggesting a dose-response relationship for Sm-TSP-2/Al with GLA-AF (pâ¯=â¯0.0001). CONCLUSIONS: Sm-TSP-2/Al with or without GLA-AF was safe and well tolerated in a Sm-naïve population. A vaccine like the one under development may represent our best hope to eliminating this neglected tropical disease.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Glucosídeos/imunologia , Imunogenicidade da Vacina , Lipídeo A/imunologia , Esquistossomose/prevenção & controle , Vacinas/imunologia , Adjuvantes Imunológicos/administração & dosagem , Adolescente , Adulto , Animais , Antígenos de Helmintos/imunologia , Estudos de Coortes , Citocinas/imunologia , Relação Dose-Resposta a Droga , Método Duplo-Cego , Feminino , Voluntários Saudáveis , Humanos , Imunoglobulina G/sangue , Masculino , Pessoa de Meia-Idade , Schistosoma mansoni , Vacinas/efeitos adversos , Adulto JovemRESUMO
Chagas disease is an important emerging disease in Texas that results in cardiomyopathy in about 30% of those infected with the parasite Trypanosoma cruzi. Between the years 2008 and 2012, about 1/6500 blood donors were T. cruzi antibody-confirmed positive. We found older persons and minority populations, particularly Hispanic, at highest risk for screening positive for T. cruzi antibodies during routine blood donation. Zip code analysis determined that T. cruzi is associated with poverty. Chagas disease has a significant disease burden and is a cause of substantial economic losses in Texas.
Assuntos
Doadores de Sangue/estatística & dados numéricos , Doença de Chagas/epidemiologia , Programas de Rastreamento , Trypanosoma cruzi/isolamento & purificação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antiprotozoários/sangue , Doença de Chagas/parasitologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Estudos Soroepidemiológicos , Fatores Socioeconômicos , Texas/epidemiologia , Adulto JovemRESUMO
In the poorest regions of the United States, especially along the Gulf Coast and in South Texas, are a group of endemic parasitic and related infections known as the neglected infections of poverty. Such infections are characterized by their chronicity, disabling features, and disproportionate impact on the estimated 46 million people who live below the U.S. poverty line. Today more Americans live in poverty than ever before in the half-century that the Census Bureau has been recording poverty rates. In association with that poverty, a group of major neglected infections of poverty have emerged in the United States. Here we describe the major neglected infections of poverty in the United States, with a brief overview of their significant epidemiological features, their links with poverty, and our approaches to their diagnosis, management, and treatment.
Assuntos
Gerenciamento Clínico , Doenças Parasitárias/terapia , Áreas de Pobreza , Viroses/terapia , Infecções por Arbovirus/terapia , Doença de Chagas/terapia , Cisticercose/terapia , Dengue/terapia , Humanos , Doenças Parasitárias/epidemiologia , Infecções por Strongylida/terapia , Texas/epidemiologia , Toxocaríase/terapia , Estados Unidos/epidemiologia , Viroses/epidemiologia , Febre do Nilo Ocidental/terapiaRESUMO
Na-ASP-2 is a major protein secreted by infective third-stage larvae (L3) of the human hookworm Necator americanus upon host entry. It was chosen as a lead vaccine candidate for its ability to elicit protective immune responses. However, clinical development of this antigen as a recombinant vaccine was halted because it caused allergic reactions among some of human volunteers previously infected with N. americanus. To prevent IgE-mediated allergic reactions induced by Na-ASP-2 but keep its immunogenicity as a vaccine antigen, we designed and tested a genetically engineered fusion protein, Fcγ/Na-ASP-2, composed of full-length Na-ASP-2 and truncated human IgG Fcγ1 that targets the negative signalling receptor FcγRIIb expressed on pro-allergic cells. The chimeric recombinant Fcγ/Na-ASP-2 protein was expressed in Pichia pastoris and shared the similar antigenicity as native Na-ASP-2. Compared to Na-ASP-2, the chimeric fusion protein efficiently reduced the release of histamine in human basophils sensitized with anti-Na-ASP-2 IgE obtained from individuals living in a hookworm-endemic area. In dogs infected with canine hookworm, Fcγ/Na-ASP-2 resulted in significantly reduced immediate-type skin reactivity when injected intradermally compared with Na-ASP-2. Hamsters vaccinated with Fcγ/Na-ASP-2 formulated with Alhydrogel(®) produced specific IgG that recognized Na-ASP-2 and elicited similar protection level against N. americanus L3 challenge as native Na-ASP-2.
Assuntos
Basófilos/imunologia , Liberação de Histamina , Imunização , Imunoglobulina E/imunologia , Fragmentos Fc das Imunoglobulinas/imunologia , Necator americanus/imunologia , Vacinação/métodos , Animais , Antígenos de Helmintos/genética , Antígenos de Helmintos/imunologia , Cricetinae , Cães , Expressão Gênica , Humanos , Hipersensibilidade/prevenção & controle , Fragmentos Fc das Imunoglobulinas/genética , Imunoglobulinas , Pichia/genética , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Pele/patologia , Vacinação/efeitos adversosRESUMO
Vaccines have been at the forefront of global research efforts to combat malaria, yet despite several vaccine candidates, this goal has yet to be realized. A potentially effective approach to disrupting the spread of malaria is the use of transmission-blocking vaccines (TBV), which prevent the development of malarial parasites within their mosquito vector, thereby abrogating the cascade of secondary infections in humans. Since malaria is transmitted to human hosts by the bite of an obligate insect vector, mosquito species in the genus Anopheles, targeting mosquito midgut antigens that serve as ligands for Plasmodium parasites represents a promising approach to breaking the transmission cycle. The midgut-specific anopheline alanyl aminopeptidase N (AnAPN1) is highly conserved across Anopheles vectors and is a putative ligand for Plasmodium ookinete invasion. We have developed a scalable, high-yield Escherichia coli expression and purification platform for the recombinant AnAPN1 TBV antigen and report on its marked vaccine potency and immunogenicity, its capacity for eliciting transmission-blocking antibodies, and its apparent lack of immunization-associated histopathologies in a small-animal model.
Assuntos
Anticorpos/imunologia , Antígenos CD13/imunologia , Insetos Vetores/enzimologia , Vacinas Antimaláricas/imunologia , Plasmodium vivax/imunologia , Animais , Anopheles/enzimologia , Anopheles/imunologia , Anopheles/parasitologia , Feminino , Humanos , Insetos Vetores/imunologia , Insetos Vetores/parasitologia , Malária/imunologia , Malária/prevenção & controle , Malária/transmissão , Camundongos , Camundongos Endogâmicos BALB C , Plasmodium berghei/imunologia , Vacinas Sintéticas/imunologiaAssuntos
Antibacterianos/administração & dosagem , Antiparasitários/administração & dosagem , Medicamentos Essenciais/administração & dosagem , Helmintíase/tratamento farmacológico , Tracoma/tratamento farmacológico , Medicina Tropical/métodos , Antibacterianos/uso terapêutico , Antiparasitários/uso terapêutico , Países em Desenvolvimento , Esquema de Medicação , Medicamentos Essenciais/uso terapêutico , Saúde Global , Helmintíase/epidemiologia , Humanos , Prevalência , Tracoma/epidemiologiaRESUMO
There is a growing emphasis on the development of vaccines against helminths (worms), and mathematical models provide a useful tool to assess the impact of new vaccines under a range of scenarios. The present study describes a stochastic individual-based model to assess the relative impact of chemotherapy and vaccination against human hookworm infection and investigates the implications of potential correlations between risk of infection and vaccine efficacy. Vaccination is simulated as a reduction in susceptibility to infection and the model includes population heterogeneities and dynamical waning of protection. To help identify appropriate measures of vaccine impact, we present a novel framework to quantify the vaccine impact on the infection-associated morbidity and introduce a measure of symmetry to study the correspondence between reduction in intensity and reduction in morbidity. Our modelling shows that, in high-transmission settings, the greatest impact of vaccination will be attained when vaccine efficacy is the greatest among individuals harbouring the heaviest worm burdens, and that the decline of morbidity primarily depends on the level of protection attained in the most at risk 8-12% of the population. We also demonstrate that if risk of infection and vaccine protection are correlated, there is not always a direct correspondence between the reduction in worm burden and in morbidity, with the precise relationship varying according to transmission setting.
Assuntos
Infecções por Uncinaria/tratamento farmacológico , Infecções por Uncinaria/prevenção & controle , Modelos Teóricos , Vacinas/imunologia , Animais , Simulação por Computador , HumanosRESUMO
This paper summarises the progress towards vaccine development against the major blood-feeding nematodes of man and livestock, the hookworms and Haemonchus contortus, respectively. The impact of the diseases and the drivers for vaccine development are summarized as well as the anticipated impact of the host immune response on vaccine design. The performance requirements are discussed and progress towards these objectives using defined larval and adult antigens, many of these being shared between species. Specific examples include the Ancylostoma secreted proteins and homologues in Haemonchus as well as proteases used for digestion of the blood meal. This discussion shows that many of the major vaccine candidates are shared between these blood-feeding species, not only those from the blood-feeding stages but also those expressed by infective L3s in the early stages of infection. Challenges for the future include: exploiting the expanding genome information for antigen discovery, use of different recombinant protein expression systems, formulation with new adjuvants, and novel methods of field testing vaccine efficacy.
Assuntos
Ancylostomatoidea/imunologia , Hemoncose/prevenção & controle , Haemonchus/imunologia , Infecções por Uncinaria/prevenção & controle , Vacinação , Ancylostomatoidea/enzimologia , Animais , Antígenos de Helmintos/imunologia , Saúde Global , Hemoncose/veterinária , Haemonchus/enzimologia , Infecções por Uncinaria/veterinária , Humanos , Peptídeo Hidrolases/metabolismoRESUMO
The role of neutrophils in mediating host inflammation was examined in mice vaccinated with living third-stage infective hookworm larvae (L3). Mice were vaccinated by oral immunization with 500 L3 (Ancylostoma caninum) once every 2 weeks for a total of three immunizations. The vaccinated mice were then challenged intraperitoneally with 2000 L3) 1 week after the final immunization. To stimulate peritoneal production of neutrophils, 2 ml of 2% glycogen were injected intraperitoneally at 16 h prior to the challenge infection. Neutrophils were found to comprise 85% of the peritoneal cell population. L3 from the challenge infection were collected and then examined at timed intervals by inverted light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Greater than a fivefold increase in the total numbers of peritoneal cells was noted in the vaccinated mice as compared to unvaccinated mice. In the peritoneal cavity of vaccinated mice, the neutrophils adhered to the L3 within 2 h, and over 55% of the L3 were surround by clusters of neutrophils to form a sausage-like sheath 4 h later. At 24-72 h after challenge, almost all of the L3 recovered from the vaccinated mice were covered with thick clusters of cells. Both SEM and TEM demonstrated extensive ultrastructural damage to the L3. In contrast, the L3 recovered from the unvaccinated mice appeared to be unaffected by neutrophils. These studies suggest that neutrophils, like macrophages, can have an important role as effector cells in L3-vaccinated mice.
Assuntos
Ancylostoma/imunologia , Ancilostomíase/imunologia , Neutrófilos/imunologia , Ancylostoma/ultraestrutura , Ancilostomíase/parasitologia , Ancilostomíase/prevenção & controle , Animais , Adesão Celular , Masculino , Camundongos , Microscopia Eletrônica de Varredura , Neutrófilos/parasitologia , Cavidade Peritoneal/parasitologia , Cavidade Peritoneal/patologia , VacinaçãoRESUMO
Hookworm is highly endemic to Hainan Province, an island located in the South China Sea. To investigate the prevalence and intensity of infection in the area, the village of Xiulongkan was surveyed between April and July 1998. A cross-sectional study was conducted in which fecal samples of 80% of the village residents (631 individuals) were tested for the presence of helminth eggs. Hookworm was the predominant intestinal helminth in Xiulongkan, where it was determined that 60% of those tested were infected. Necator americanus was the predominant species of hookworm in this population. The prevalence of hookworm increased with age, and then leveled to a plateau for ages 41 yr and up. This observation was in contrast to infections with Ascaris lumbricoides, where the highest prevalences occurred among school-aged children. Women had a significantly higher prevalence of hookworm than men and this difference emerged in early adulthood. The intensity of hookworm infection also significantly increased with age, with the highest intensity infections occurring among middle-aged and elderly residents. Females were more likely to have moderate or heavy infections, whereas males were more likely to have light infections. The rates of hookworm transmission are particularly high among the middle-aged and elderly residents of Xiulongkan.
Assuntos
Necator americanus , Necatoríase/epidemiologia , Adolescente , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , China/epidemiologia , Feminino , Geografia , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Prevalência , População Rural , Fatores SexuaisRESUMO
The zoonotic ascarid Toxocara has been suggested as a possible etiologic agent of asthma. We conducted a clinic-based case-control study to examine whether the zoonotic infection acquired by ingesting Toxocara eggs is associated with asthma in children. Blood samples were collected from children aged 2-15 years, 95 of whom had asthma and 229 of whom did not have asthma. Risk factors for asthma and Toxocara infection were assessed by a questionnaire given to each child's parent or legal guardian. Blood samples were tested for the presence of Toxocara antibodies, using an enzyme-linked immunosorbent assay. No significant association was found between Toxocara infection and asthma. Significant associations were found between asthma and risk factors and between Toxocara infection and risk factors. High prevalence of Toxocara infection was noted among Hispanic children of Puerto Rican descent.
Assuntos
Asma/parasitologia , Toxocara/fisiologia , Adolescente , Animais , Anticorpos Anti-Helmínticos/sangue , Estudos de Casos e Controles , Criança , Pré-Escolar , Exposição Ambiental , Feminino , Humanos , Masculino , Fatores de Risco , Toxocara/imunologia , Toxocaríase/sangue , Toxocaríase/imunologiaRESUMO
OBJECTIVE: To analyze the immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum with antigens of various species hookworms at different developmental stages. METHODS: SDS-PAGE and ELIB technique were employed in the study. RESULTS AND CONCLUSION: The protein component of Ac-rAsp-1 was 45 kDa, its immune serum can recognize the antigens of Ac-L3 and Ac-rAsp-1 protein, but not react to the antigens of Ad-A, Ad-L3, Na-A, Ac-A, Nb-A and Ac-rAsp-2 protein. The protein component of Ac-rAsp-2 was 24 kDa, its immune serum can recognize the antigens of Ad-A, Ad-L3, Na-A, Ac-A, Ac-L3 and Ac-rAsp-2 protein, but not react to the antigens of Nb-A and Ac-rAsp-1 protein.
Assuntos
Ancylostoma/imunologia , Proteínas de Helminto/imunologia , Soros Imunes/imunologia , Animais , Antígenos de Helmintos/imunologia , Cães , Ratos , Ratos Sprague-Dawley , Proteínas Recombinantes/imunologiaRESUMO
Vaccination of mice with alum-precipitated recombinant Ancylostoma secreted protein-1 from the canine hookworm Ancylostoma caninum (Ac-ASP-1) results in protection against A. caninum larval challenge. Vaccine protection is manifested by host reductions in hookworm burden compared to control mice. The goal of this study was to determine whether ASP antigens cloned and expressed from different hookworm species will cross protect against A. caninum larval challenge. Cross-species protection against A. caninum challenge infections was observed with immunizations using recombinant ASP-1 from the human hookworms Ancylostoma duodenale and Necator americanus. However, the degree of protection was proportional to the extent of amino acid sequence homology between the ASP immunogen used for vaccination and the Ac-ASP-1 produced by the challenge larval strain. Vaccine protection was noted to decrease significantly as amino acid sequence homologies diverged 10% or more. It was also determined that Ac-ASP-2, a molecule cloned from A. caninum having 55% amino acid sequence homology to the C-terminus of Ac-ASP-1, did not elicit vaccine protection. These observations were partly reflected in the titer of antibodies that recognize Ac-ASP-1. The studies reported here will help to design immunogenic peptide vaccines based on the sequence divergence of hookworm ASPs.
Assuntos
Ancylostoma/imunologia , Proteínas de Helminto/imunologia , Necator americanus/imunologia , Vacinas Sintéticas/imunologia , Animais , Anticorpos Anti-Helmínticos/sangue , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Peso Molecular , Proteínas Recombinantes/imunologia , VacinaçãoRESUMO
Conventional drug chemotherapy against human schistosomiasis currently relies on treatment with praziquantel to eliminate adult schistosome worm pairs. The use of praziquantel for control purposes is limited, however, by high rates of post-treatment re-infection with subsequent parasite egg deposition and host end-organ damage. Artemether, a methyl ether derivative of the anti-malarial drug quinghaosu, was discovered recently to also have anti-schistosomal properties. Because artemether selectively targets the larval migratory stages of the parasite, known as schistosomulae, it blocks the development of ovipositing adult schistosome worm pairs in the vasculature. On this basis, we have since shown in clinical trials conducted in China that artemether has proven benefit as an agent for chemoprophylaxis. In vivo studies using laboratory animals suggest that artemether causes damage to the tegument and musculature of schistosomulae. Artemether may exert its helminthotoxic effect through synergy with hemin or related heme-containing compounds. Schistosomes recovered from artemether treated laboratory animals have increased glycogen phosphorylase activity, but decreased glucose uptake. These findings may account for their decreased glycogen content, relative to schistosomes recovered from untreated laboratory animals. The artemether-damaged schistosomes also have decreased activities of a number of enzymes and enzyme systems, including glycolysis. This might suggest common pathways by which artemether may target human parasites that live in the bloodstream.
Assuntos
Artemisininas , Esquistossomose/prevenção & controle , Esquistossomicidas/uso terapêutico , Sesquiterpenos/uso terapêutico , Animais , Artemeter , China/epidemiologia , Feminino , Humanos , Masculino , Camundongos , Plasmodium/efeitos dos fármacos , Schistosoma/efeitos dos fármacos , Esquistossomose/tratamento farmacológico , Esquistossomose/epidemiologia , Esquistossomicidas/farmacologia , Sesquiterpenos/farmacologiaRESUMO
Anti-hookworm antibody serologic responses were measured in residents of an Anhui provincial Chinese village where Ancylostoma duodenale is the predominant hookworm. Antibody responses were measured against either soluble infective third-stage larval (L3) or adult antigens. Immunoglobulins of the IgG class, especially IgG4 correlated with both the prevalence and intensity of A. duodenale hookworm infections. In contrast, there was an inverse correlation with IgM, but no correlation with IgA or IgE. Circulating IgG4 antibody responses might serve as a surrogate marker for active A. duodenale hookworm infection.
Assuntos
Ancilostomíase/epidemiologia , Anticorpos Anti-Helmínticos/sangue , Saúde da População Rural , Ancilostomíase/sangue , Ancilostomíase/imunologia , Ancilostomíase/patologia , China/epidemiologia , Ensaio de Imunoadsorção Enzimática , Humanos , Prevalência , Estudos SoroepidemiológicosRESUMO
Hookworm infection is a major parasitic cause of morbidity in the developing nations of the tropics. Development of a genetically engineered vaccine would be a useful tool in the control of this infection in highly endemic areas. Recombinant polypeptides belonging to the Ancylostoma secreted protein (ASP)-1 family have shown promise for reducing hookworm burdens after larval challenge infections in mice. Typically, these polypeptides are expressed in Escherichia coli and administered as an alum precipitate. Vaccine protection is antibody dependent. It is anticipated that a cocktail of different recombinant hookworm antigens may be required in order to effectively prevent heavy hookworm infections and disease. The progress of this work has been hampered by the absence of both a convenient laboratory animal with which to study hookworm infections resembling human infection, as well as the lack of easy availability of native hookworm antigens. In addition, useful human serologic correlates of antihookworm immunity are still poorly defined.
Assuntos
Infecções por Uncinaria/prevenção & controle , Vacinas Sintéticas , Ancylostoma/imunologia , Ancylostomatoidea/imunologia , Ancilostomíase/prevenção & controle , Animais , Antígenos de Helmintos/imunologia , Proteínas de Helminto/imunologia , Humanos , Camundongos , Modelos Imunológicos , Necator americanus/imunologia , Necatoríase/prevenção & controle , Vacinas Sintéticas/imunologiaRESUMO
Hookworm infection as well as other intestinal nematodiases are endemic to Sichuan Province in China. In order to research the prevalence and intensity of these infections we visited two villages in Hejiang County (southern Sichuan Province) and Santai County (northwestern Sichuan Province) between July and October of 1997. Fecal examinations were performed on adult villagers over the age of 15 years (currently children under this age are dewormed annually with anthelmintic drugs). Among 310 residents of Lugao Village (Hejiang County), 87, 63 and 60% were infected with hookworm, Ascaris or Trichuris, respectively. The prevalence of hookworm determined to rise linearly with age (r = 0.97). High intensity infections with hookworm still occur in this region as 22% of the residents have over 3000 eggs per gram (PEG) of feces as determined by quantitative egg counts. The majority of these individuals harbored mixed infection with Necator americanus and Ancylostoma duodenale, although the former predominated when adult hookworms were collected from 30 village residents treated with pyrantel pamoate. In contrast, among the 334 Xinjian villagers examined (Santai County) the majority harbored predominantly light hookworm infections--66.1% of the residents has less than 400 EPG by quantitative fecal examination and only 3.7% exhibited greater than 3000 EPG. Again, N. americanus was the predominant hookworm seen after worm expulsion. We have round that despite economic development which is occurring in some parts of China, significant hookworm infections and clinical hookworm anemia still exist in areas of Sichuan Province. In Hejiang County we found that the intensity of hookworm infection has actually risen within the last 10 years. Hookworm is a medical problem among the elderly in Sichuan.
Assuntos
Ancylostoma/isolamento & purificação , Ancilostomíase/epidemiologia , Necator americanus/isolamento & purificação , Necatoríase/epidemiologia , Adolescente , Adulto , Ancilostomíase/tratamento farmacológico , Ancilostomíase/parasitologia , Animais , Antinematódeos/uso terapêutico , Ascaríase/epidemiologia , Ascaríase/parasitologia , China/epidemiologia , Fezes/parasitologia , Feminino , Humanos , Masculino , Necatoríase/tratamento farmacológico , Necatoríase/parasitologia , Contagem de Ovos de Parasitas , Prevalência , Pirantel/uso terapêutico , Saúde da População Rural , Tricuríase/epidemiologia , Tricuríase/parasitologiaRESUMO
Vaccination of mice with either third-stage Ancylostoma caninum infective hookworm larvae (L3) or alum-precipitated recombinant Ancylostoma secreted protein 1 from A. caninum (Ac-ASP-1) results in protection against hookworm challenge infections. Vaccine protection is manifested by reductions in lung hookworm burdens at 48 h postchallenge. Mice actively immunized 4 times with Ac-ASP-1 also exhibited reductions in hookworm burden in the muscles. Hookworm burden reductions from Ac-ASP-1 immunization were associated with elevations in all immunoglobulin subclasses, with the greatest rise observed in host IgG1 and IgG2b. The addition of a fourth immunization resulted in even higher levels of IgG and IgE. In contrast, L3-vaccinated mice exhibited marked elevations in IgG1 and IgM, including anti-Ac-ASP-1 IgM antibody. Passive immunization with pooled sera from recombinant Ac-ASP-1-vaccinated mice also resulted in lung hookworm burden reductions. It is hypothesized that recombinant Ac-ASP-1 vaccinations elicit antibody that interferes with parasite larval migration.