Prenatal and postnatal exposure to heavy metals in PM2.5 and autism spectrum disorder.

Autism spectrum disorder (ASD) is a group of neurodevelopmental disorders, and its incidence is increasing over time. Although several environmental factors have been suspected to be risk factors for ASD, studies on the effects of airborne heavy metals on newly developed ASD are still limited. We conducted a large birth cohort study of 168,062 live term births in Taichung during 2004-2011 to assess the association of heavy metals in particulate matter with an aerodynamic diameter less than 2.5 µm (PM2.5) with ASD, and identify sensitive time windows during prenatal and postnatal periods. Heavy metals, including arsenic (As), cadmium (Cd), mercury (Hg), and lead (Pb) in PM2.5, were estimated using the Weather Research and Forecasting/Chem (WRF/Chem), inserted from the top 75 emission sources for the module. The association between childhood ASD and 4 metals were analyzed from pregnancy to 9 months after birth. The Cox proportional hazard model with a distributed lag nonlinear model (DLNM) was used to estimate the association between heavy metals in PM2.5 and ASD. We identified 666 incident ASD cases in 168,062 participants. A positive association between Hg and ASD was found at 9 months after birth (Hazard Ratio: 1.63; 95% CI: 1.13-2.36). According to the DLNM, there was an increased risk of exposure to Hg during 10-25 weeks after birth, and decreased risk of exposure to Hg during gestational weeks 4-6. Exposure to As and Hg on the risk of ASD were significantly stronger in low birth weight infants (<2500 g) than in those of birth weight ≥2500 g during postnatal period. Postnatal exposure to Hg in PM2.5 may associate with increased ASD incidence. Infants with low birth weight and exposure to As and Hg in PM2.5 are more likely to develop ASD.

The impact of maternal influences on childhood obesity.

There was a lack of detailed information about maternal influences on their children's body mass index (BMI) in Taiwan. The aim of this study was to find the evidence to describe how mothers' factors could affect their 2 to 9-year-old children's BMI, with data collected from May 2021 to June 2021. Anonymous self-administered questionnaires were completed by 1035 participants from Taiwan's six metropolitan cities and eight counties. After controlling for children's factors, such as number of children in a family, children's constitution, children's age and gender, hierarchical regression models were used to analyze the effects of five maternal factors on their children's BMI: maternal BMI, age, education level, monthly household income, and marital status (single parent or not). The results were found as follow: maternal BMI [ß = .24], maternal educational level [ß = -.141], and monthly household income [ß = .071], significantly (p < 0.05) influenced their children's BMI. Higher maternal BMI was associated with a higher children's BMI. Mothers with lower levels of education background were more likely to have children with a higher BMI. Monthly household income was a positive factor influencing children's BMI. In conclusion, this study is the first detailed description of maternal influences on their 2-9 years old children's BMI in Taiwan. Although the study could not cover all of the factors influencing Taiwan's childhood obesity, we have discovered maternal BMI, education level, and monthly household income were significant factors associated with children's BMI.

The basic reproductive number estimated from a Mycoplasma conjunctivae outbreak in a dairy goat farm.

Clinicians can evaluate the relevance of an outbreak based on its basic reproductive number (R0). So far there has been no report on the R0 of Mycoplasma conjunctivae which is a major cause of goats' conjunctivitis in Taiwan. The present study sought to investigate an outbreak of infectious keratoconjunctivitis (IKC) by Mycoplasma conjunctivae (MC) in an indoor dairy goat barn. The epidemiological curve was recorded to build a susceptible-infected-recovered model and to estimate the R0 by three methods In the investigated goat barn, 60% (31/55) goats showed degrees of IKC signs. The number of infected animals increased quickly after 15days, but slowed down after 41days. The sick goats began to recover after 30days. The epidemic fully stopped after 81days. The estimated R0 ranged from 1.35 to 4.46. In summary, this is the first MC report in Taiwan, and the first one to estimate the R0 of MC.

KMUP-1 suppresses RANKL-induced osteoclastogenesis and prevents ovariectomy-induced bone loss: roles of MAPKs, Akt, NF-κB and calcium/calcineurin/NFATc1 pathways.

BACKGROUND: KMUP-1 is a xanthine derivative with inhibitory activities on the phosphodiesterase (PDE) 3,4 and 5 isoenzymes to suppress the degradation of cyclic AMP and cyclic GMP. However, the effects of KMUP-1 on osteoclast differentiation are still unclear. In this study, we investigated whether KMUP-1 inhibits osteoclastogenesis induced by RANKL in RAW 264.7 cells and bone loss induced by ovariectomy in mice, and the underlying mechanisms. PRINCIPAL FINDINGS: In vitro, KMUP-1 inhibited RANKL-induced TRAP activity, the formation of multinucleated osteoclasts and resorption-pit formation. It also inhibited key mediators of osteoclastogenesis including IL-1ß, IL-6, TNF-α and HMGB1. In addition, KMUP-1 inhibited RANKL-induced activation of signaling molecules (Akt, MAPKs, calcium and NF-κB), mRNA expression of osteoclastogensis-associated genes (TRAP, MMP-9, Fra-1, and cathepsin K) and transcription factors (c-Fos and NFATc1). Furthermore, most inhibitory effects of KMUP-1 on RANKL-mediated signal activations were reversed by a protein kinase A inhibitor (H89) and a protein kinase G inhibitor (KT5823). In vivo, KMUP-1 prevented loss of bone mineral content, preserved serum alkaline phosphate and reduced serum osteocalcin in ovariectomized mice. CONCLUSIONS: KMUP-1 inhibits RANKL-induced osteoclastogenesis in vitro and protects against ovariectomy-induced bone loss in vivo. These effects are mediated, at least in part, by cAMP and cGMP pathways. Therefore, KMUP-1 may have a role in pharmacologic therapy of osteoporosis.