RESUMO
The primary challenges in fruit and vegetable preservation include extending storage duration while preserving sensory quality and nutritional value. In this study, sesbania gum (SG) was oxidized to prepare oxidized sesbania gum (OSG). An OSG/ZnO composite film was subsequently prepared, combining OSG, sodium carboxymethyl cellulose (CMC), and nano-zinc oxide (nano-ZnO). The preparation technology was determined via a response surface optimization experiment. When the addition amount of nano-ZnO exceeded 0.3 mg/mL, the composite films exhibited an antibacterial rate of over 90% against E. coli and S. aureus. For wampee (Clausena lansium (Lour.) Skeels) preservation, a OSG/ZnO-0.3 film was directly applied as a coating. The findings demonstrated favorable results in terms of the rate of rotting, soluble solids, and titrable acidity, effectively prolonging wampee fruit storage. This suggests the potential of an OSG composite film with nano-ZnO as a promising fruit packaging material, thereby expanding the application of SG and wampee fruit preservation.
RESUMO
Intercropping green manure (GM) may be a good solution to the problems of acid soil in tropical plantations. Soil organic nitrogen (No) may change due to the application of GM. A three-year field experiment was conducted to determine the effect of different utilization patterns of Stylosanthes guianensis GM on soil No fractions in a coconut plantation. Three treatments were set: no GM intercropping (CK), intercropping and mulching utilization pattern (MUP), and intercropping and green manuring utilization pattern (GMUP). The content dynamics of soil total N (TN) and soil No fractions including of non-hydrolysable N (NHNo) and hydrolyzable N (HN) in the cultivated soil layer was examined. The results showed that after three years of intercropping, the TN content of the MUP and GMUP treatment was 29.4% and 58.1% respectively higher (P < 0.05) than those of the initial soil, and the No fractions content of GMUP and MUP treatment was 15.1%-60.0% and 32.7%-111.0% higher (P < 0.05) than those of the initial soil. The further results indicated that after three years of intercropping, compared with CK, GMUP and MUP could increase the content of TN by 32.6% and 61.7% respectively, and No fractions content was also increased by 15.2%-67.3% and 32.3%-120.3%% respectively (P < 0.05). The No fractions content of GMUP treatment was 10.3%-36.0% higher than those of MUP treatment (P < 0.05). These results indicated that intercropping Stylosanthes guianensis GM could significantly increase the soil N including of the TN an No fractions content, and the GMUP was more effective than MUP, therefore, GMUP is a better GM utilization pattern to improve the soil fertility and should be popularized in the tropical fruit plantation.
Assuntos
Fabaceae , Solo , Agricultura/métodos , Nitrogênio/análise , Esterco , Cocos , Fertilizantes , ChinaRESUMO
The actin-depolymerizing factor 4 gene (ADF4) of Arabidopsis thaliana was cloned and sequenced (Figs.1, 2). The plant expression vector with ADF4 was constructed and transformed into tobacco by Agrobacterium tumefaciens. Molecular identification showed that the ADF4 gene was integrated into the genome of tobacco and expressed in transgenic tobaccos assayed by PCR and RT-PCR (Fig.3). Expression of an Arabidopsis thaliana ADF4 gene in tobacco resulted in morphological change of plants. The effects of ADF4 on transgenic tobaccos growth were as follows: the hypocotyls of transgenic plants were wavy, especially in darkness, whereas those of the control were straight (Fig.4A); the root hairs of transgenic plants were less than the control, and they were also wavy (Fig.4B); the parenchyma cells of transgenic plants were larger than the control and the arrangement of vascular bundle was out of order (Fig.4C); the flowering time of T(2) line was at least 7 days later than the control; the pollen tubes of transgenic plants were shorter than those of the control (Fig.4D).