Structure and characteristics of the plant-frugivore bird network from the Guilin Botanical Garden.

The interaction between plants and frugivores is crucial to ecosystem function and community diversity. However, little is known about the interaction between plants and frugivorous bird species in urban green spaces. We observed interactions between plants and frugivorous birds in the Guilin Botanical Garden for one year and determined the structure and characteristics of the interaction network. We also analyzed the impact of species traits on their network roles. Interactions between 14 frugivorous birds and 13 fruit plant species were recorded in the study area. Autumn interactions comprised 38.79% of the overall network, and winter interactions comprised 33.15%. The modularity (Q, z-score) of the network was higher in autumn; the weighted nestedness (wNODF, z-score) and interaction evenness (E2 , z-score) of the network were higher in winter; the connectance (C, z-score) and interaction diversity (z-score) of the network were higher in spring; and the specialization (H2', z-score) of the network was higher in summer. The observed network showed lower C, lower interaction H2 , lower E2 , lower wNODF, higher H2' and higher Q when compared to the random networks. The bird species most important to network stability were Hemixos castanonotus, Parus venustulus, and Pycnonotus sinensis. The most important plant species were Alocasia macrorrhiza, Cinnamomum camphora, and Machilus nanmu. Of all the bird and plant traits included in this study, only plant color had a significant impact on species strength, with black fruit having a higher species strength. Our results suggest that interaction networks in urban green spaces can be temporally complex and variable and that a network approach can be an important monitoring tool for detecting the status of crucial ecosystem functions.

Complete mitogenome of the Common Koel Eudynamys scolopaceus Linnaeus 1758 (Aves: Cuculidae).

The Common Koel Eudynamys scolopaceus Linnaeus 1758, belongs to the family Cuculidae and order Cuculiformes. This omnivorous bird exhibits obligate nest parasitism. Here, the complete mitogenome of E. scolopaceus was determined and phylogenetically compared with those of other Cuculidae species. The newly sequenced complete mitogenome was a circular DNA molecule with a size of 17,610 bp (OM115963). This mitogenome had a higher A + T content (57.58%) than G + C content (42.42%). Phylogenetic analysis revealed that E. scolopaceus was most closely related to Eudynamys taitensis and the genus Cuculus, providing useful molecular information for further research on the phylogeny of the family Cuculidae.

First determination and analysis of the complete mitochondrial genome of X-ray tetra Pristella maxillaris (Ulrey, 1894) (Actinopteri, Characidae).

The complete mitochondrial genome of the X-ray tetra (Pristella maxillaris, Ulrey, 1894) was determined by using next-generation sequencing technology, and its mitochondrial genome characteristics were analyzed. The sequence total length was 16,753 bp, and the A + T content was 57.44%. The position and composition of the 37 genes were consistent with those of other Characidae species in this family. There are 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region. Except for ND2 and COX1, which use ATT and GTG as start codons, respectively, all other protein-coding genes use ATG as the start codon. COX1 uses AGG as the stop codon; ATP6 and COX3 use incomplete TA as the stop codon; COX2 and ND4 use incomplete T as the stop codon; ATP8 uses TAG as the stop codon, and the other seven protein-coding genes use TAA as the stop codon. Based on the concatenated nucleotide sequences of 13 protein-coding genes from 18 Characidae species, phylogenetic analysis revealed that P. maxillaris belongs to the family Characidae and is most closely related to Hyphessobrycon amandae. Determining the mitogenomes of P. maxillaris improves our understanding of the phylogeny and evolution of Characidae.

Synthesis, Characterization, DNA/HSA Interactions, and Anticancer Activity of Two Novel Copper(II) Complexes with 4-Chloro-3-Nitrobenzoic Acid Ligand.

The purpose of this study was to identify new metal-based anticancer drugs; to this end, we synthesized two new copper(II) complexes, namely [Cu(ncba)4(phen)] (1) and [Cu(ncba)4(bpy)] (2), comprised 4-chloro-3-nitrobenzoic acid as the main ligand. The single-crystal XRD approach was employed to determine the copper(II) complex structures. Binding between these complexes and calf thymus DNA (CT-DNA) and human serum albumin (HSA) was explored by electronic absorption, fluorescence spectroscopy, and viscometry. Both complexes intercalatively bound CT-DNA and statically and spontaneously quenched DNA/HSA fluorescence. A CCK-8 assay revealed that complex 1 and complex 2 had substantial antiproliferative influences against human cancer cell lines. Moreover, complex 1 had greater antitumor efficacy than the positive control cisplatin. Flow cytometry assessment of the cell cycle demonstrated that these complexes arrested the HepG2 cell cycle and caused the accumulation of G0/G1-phase cells. The mechanism of cell death was elucidated by flow cytometry-based apoptosis assays. Western blotting revealed that both copper(II) complexes induced apoptosis by regulating the expression of the Bcl-2(Bcl-2, B cell lymphoma 2) protein family.

Synthesis, crystal structures, DNA interactions, and antitumor activity of two new dinuclear copper(ii) complexes with thiazole ligand.

Two new dinuclear copper(ii) complexes, [Cu(ambt)2(cnba)4] (1) and [Cu(ambt)2(clba)4] (2) were synthesized with 2-amino-6-methoxybenzothiazole (ambt) as the main ligand. The structures of the two complexes were characterized by single-crystal XRD. The binding between CT-DNA (calf thymus DNA) and the complexes was evaluated by viscometry, electronic absorption, and fluorescence spectroscopy, and the binding constants were calculated using the Stern-Volmer equation. The complexes were intercalatively bound to CT-DNA, and [Cu(ambt)2(clba)4] having a greater binding constant than [Cu(ambt)2(cnba)4]. The two complexes had better antitumor properties against HepG2 (human hepatocellular carcinoma), A549 (human lung carcinoma), and HeLa (human cervical carcinoma) tumor cell lines than their respective ligands and cisplatin. Furthermore, [Cu(ambt)2(clba)4] had a stronger inhibitory ability on the three types of tumor cells than [Cu(ambt)2(cnba)4], which is congruent with the binding power of the complexes with DNA. Flow cytometry revealed that [Cu(ambt)2(cnba)4] and [Cu(ambt)2(clba)4] could trigger apoptosis or necrosis, arrest the HepG2 cell cycles, and cause G0/G1-phase cells to accumulate.