Physiological responses and agreement between two traditional lunging protocols for physical fitness training in untrained ponies.

Lunging is a training technique commonly performed in a round pen or on a lunge line. This study investigated the effort intensity, autonomic responses, and method agreement in applying different lunging protocols to untrained ponies. Sixteen untrained ponies (aged 13.6 ± 6.3 years and weighing 358 ± 47.4 kg) were studied. Each pony underwent the same research-focused lunging program on a lunge line and, subsequently, in a round pen with a two-day interval between sessions. The heart rate variability (HRV) and effort intensity, indicated as a percentage of maximum heart rate (%HRmax), were determined pre-lunging, during lunging at distinct gaits, and at 30 min intervals for 120 min post-lunging. The correlation and method agreement between the two lunging methods were analysed. Ponies ran faster and covered longer distances during exercise on a lunge line than in a round pen. The effort intensity during cantering reached moderate levels (75.10 ± 2.39 % HRmax) with occasional high-intensity levels (88.07 ± 1.29 % HRmax) via both lunging methods. The HRV reached a minimum during cantering and returned to the baseline 120 min post-lunging. The HRV parameters (SDNN, RMSSD, LF, HF, SD1, and SD2) were strongly correlated (rp ≥ 0.97 and p < 0.001 for all) with a large correlation effect size (R2 > 0.85) and excellent agreement (average differences were within mean ± 1.96 SD) between the two lunging methods. In conclusion, lunging can elicit high physiological responses in untrained ponies, and both lunging methods demonstrate excellent agreement and yield similar physiological responses in these ponies.

Cortisol levels, heart rate, and autonomic responses in horses during repeated road transport with differently conditioned trucks in a tropical environment.

Horse's stress responses have been reported during road transport in temperate but not tropical environments. Therefore, this study measured cortisol levels, heart rate (HR), and heart rate variability (HRV) in horses during medium-distance road transport with different truck conditions in a tropical environment. Six horses were repeatedly transported in either air-conditioned trucks with full (ATF) or space (ATS) loads or non-air-conditioned trucks with full (N-ATF) and space (N-ATS) loads. Blood cortisol was determined beforehand and 5, 30, and 90 minutes post-transport. HR and HRV were assessed pre-transport and at 15-minute intervals until 90 minutes post-transport. Cortisol levels increased significantly in N-ATS horses (but non-significantly in ATF, ATS, and N-ATF horses) at 5 minutes post-transport and returned to baseline by 30 minutes post-transport. Predominant parasympathetic nervous system (PNS) activity was observed during the first few hours and returned to baseline until the destination was reached. A recurrent, increased PNS activity was detected post-transport. Interaction effects of air condition-by-loading condition-by-time, air condition-by-time, and separate effects of air condition and time were observed on HR and various HRV variables during transport. A transient increase in beat-to-beat intervals, coinciding with decreased HR, was observed in ATF horses. The PNS index increased, corresponding to a decreased sympathetic nervous system index, in ATS horses during transport. We suggest that medium-distance road transport causes no stress for transport-experienced horses in a tropical environment. Air and loading conditions impacted hormonal and autonomic modulation, causing different responses in horses transported in differently conditioned trucks.

Heart rate and heart rate variability in horses undergoing hot and cold shoeing.

Heart rate variability (HRV) is a frequently used indicator of autonomic responses to various stimuli in horses. This study aimed to investigate HRV variables in horses undergoing cold (n = 25) or hot (n = 26) shoeing. Multiple HRV variables were measured and compared between horses undergoing cold and hot shoeing, including the time domain, frequency domain, and nonlinear variables pre-shoeing, during shoeing, and at 30-minute intervals for 120 minutes post-shoeing. The shoeing method interacted with time to change the HRV variables standard deviation of RR intervals (SDNN), root mean square of successive RR interval differences (RMSSD), very-low-frequency band, low-frequency band (LF), the LF to high-frequency band ratio, respiratory rate, total power, standard deviation perpendicular to the line of identity (SD1), and standard deviation along the line of identity (SD2). SDNN, RMSSD, and total power only increased 30 minutes after hot shoeing (all p < 0.05). Triangular interpolation of normal-to-normal intervals (TINN) and the HRV triangular index increased during and up to 120 minutes after hot shoeing (p < 0.05-0.001). TINN increased only during cold shoeing (p < 0.05). LF increased 30 and 60 minutes after hot shoeing (both p < 0.05). SD1 and SD2 also increased 30 minutes after hot shoeing (both p < 0.05). SDNN, TINN, HRV triangular index, LF, total power, and SD2 were higher in hot-shoed than cold-shoed horses throughout the 120 minutes post-shoeing. Differences in HRV were found, indicating increased sympathovagal activity in hot shoed horses compared to cold shoed horses.

Physiological stress responses in horses participating in novice endurance rides.

Horses participating in endurance events encounter enormous physical challenges. Heart rate (HR) and heart rate variability (HRV) have been reported before and after endurance rides, but these have not been determined during the rides. Moreover, the modulation in HRV and haematology in horses with different ride results (completed a course or disqualified due to irregular gait) have not been elucidated. Therefore, this study aimed to investigate changes in HR, HRV, and haematological parameters during novice endurance rides and to compare these parameters between horses that successfully completed the course (SC) or were disqualified for irregular gait (FTQ-GA). Beat-to-beat (RR) intervals of 16 healthy horses (aged 6-14 years) were recorded before and throughout the approximately 40 km endurance event. Blood samples were taken at the pre-ride inspection and after passing each veterinary inspection. HRV and haematology measures were determined from nine SC and seven FTQ-GA horses. Horses with different ride results demonstrated distinctive physiological stress responses. Increases in PCV, RBC, WBC and neutrophils after completing the ride were found only in SC horses, implying that they were ridden with greater effort than FTQ-GA horses. A reduction in HRV during warm-up, followed by a significant reduction during the first and second riding phases, was observed. HRV returned to baseline at the compulsory rest period of both phases. FTQ-GA horses experienced lower RR intervals, RR triangular index, modified deceleration capacity, very-low-frequency band, and parasympathetic nervous system index, coinciding with higher HR and sympathetic nervous system and stress indices than SC horses. These results indicated that endurance horses revealed a shift toward sympathetic activity during the ride. Lower parasympathetic activity in FTQ-GA horses suggests they were under more stress or discomfort than SC horses in novice endurance rides. These results have welfare implications, indicating the need for additional rest breaks in FTQ-GA horses.

Comparison of daily heart rate and heart rate variability in trained and sedentary aged horses.

Athletic horses are usually retired from equestrian competition at an advanced age. Aged horses may then continue to have regular exercise or are sedentary. The comparison of autonomic regulation between sedentary aged horses (SAH) and trained aged horses (TAH) after retirement remains unpublished. This study compared daily heart rate (HR) and heart rate variability between 11 SAH and 11 TAH over 22 h (07.00 h to 05.00 h on an alternate day) and during the day and night. An increase in mean beat-to-beat (RR) intervals (p < 0.01-0.0001) and parasympathetic nervous system (PNS) index (p < 0.05-0.0001), corresponding to the decreased HR and sympathetic nervous system (SNS) index (p < 0.01-0.0001), was observed at night. Compared to SAH, lowered mean HR and SNS index, corresponding to elevated mean RR intervals and very-low-frequency bands, were marked in TAH during the day (p < 0.05 for all variables). Whereas the standard deviation of the RR intervals index was higher in TAH than in SAH during the day and night (p < 0.05 for both periods). It was suggested that aged horses with different physical activities revealed distinct autonomic responses. TAH demonstrated higher HRV than SAH during the day. A decreased HR in TAH is prone to result from a reduced SNS role, leading to a shift toward more PNS activity during the day. More research is warranted to determine to what extent the autonomic regulation is modulated by accompanying higher exercise volume or programmed exercise in aged horses.

Hematological and physiological responses in polo ponies with different field-play positions during low-goal polo matches.

Strenuous exercise in traditional polo matches creates enormous stress on horses. Hematological and physiological measures may vary across different field-play positions. This study aimed to investigate the effort intensity and the impact of exertion on hematology and heart rate variability (HRV) in polo ponies with different positions. Thirty-two ponies, divided equally into eight teams, were studied. Each comprises forwards (number 1), midfielders (numbers 2 and 3), and defenders (number 4). Team pairs played the first chukka in four low-goal polo matches. Percent maximum heart rate (%HRmax), indicating ponies' effort intensity, was classified into five zones, including zones 1 (<70%), 2 (70-80%), 3 (80-90%), 4 (90-95%) and 5 (>95%). Hematological and HRV parameters were determined before, immediately after, and at 30-minute intervals for 180 minutes after chukkas; HRV variables were also obtained during warm-up and exercise periods. Results indicated that the number two ponies spent more time in zone 4 (p < 0.05) but less in zone 2 (p < 0.01) than the number four ponies. Cortisol levels increased immediately and 30 minutes afterward (p < 0.0001 for both) and then returned to baseline 60-90 minutes after exertion. Other measures (Hct, Hb, RBC, WBC, neutrophils, and CK enzyme) increased immediately (p < 0.0001 for all) and lasted at least 180 minutes after exertion (p < 0.05-0.0001). HRV decreased during the chukka until approximately 90 minutes afterward (p < 0.05-0.0001). The stress index increased during the chukka and declined to baseline at 60 minutes in number 1-3 ponies but lasted 90 minutes in those at number four. Effort intensity distribution differed among field-play positions. Decreased HRV indicated reduced parasympathetic activity during exercise, extending to 90 minutes after exertion in polo ponies. Defenders seem to experience more stress than those in other positions.

Effect of Exercise in a Vector-Protected Arena for Preventing African Horse Sickness Transmission on Physiological, Biochemical, and Behavioral Variables of Horses.

During an African horse sickness (AHS) outbreak, horses were able to exercise daily in a net-covered arena, yet the physiological responses to exercise in a netted arena was unknown. In a cross-over study design, eight horses performed a 39-minute aerobic exercise in conventional (CA) and vector-protected arenas (VPA). Horses were slower in some gaits and covered less distance in the VPA arena (P < .01). Cortisol release, hematology, and heart rate variability (HRV) were also examined. An interaction between the riding arena and time was observed in hematocrit (P = .0013), hemoglobin (P = .0012), and red blood cell count (P = .0027) and HRV variables, including mean beat-to-beat (RR) intervals (P < .0001), mean heart rate (P < .0001), sympathetic nervous system (SNS) index (P = .0038) and parasympathetic nervous system (PNS) index (P < .0001). Cortisol concentrations increased during exercise and 30 minutes postexercise in both arenas. Hematocrit, hemoglobin, and red blood cell count increased immediately postexercise in horses in VPA while remaining high from immediate post-exercise to 60 minutes postexercise in horses in CA. HRV decreased during exercise and was not different between horses in both arenas, but a higher RR interval and PNS index, corresponding to lower heart rate and SNS index, were detected during 30 to 60 minutes postexercise in horses in the VPA compared to the CA. Riding horses in different arenas impacted hematological and HRV variables. The greater RR intervals and PNS index, coinciding with the lower SNS index and heart rate, indicated parasympathetic dominance post-exercise in horses in VPA compared to CA.

Strategic palmar trimming before conventional shoeing shows potential for managing underrun heels in horses.

Background: Underrun heels are among the most hoof imbalances, causing poor performance in hoses. Several corrective shoeing protocols have been performed, but the outcomes have yet to be consistent. In this study, we proposed an alternative trimming protocol for managing underrun heels: strategic palmar trimming before conventional shoeing. Therefore, the current study aimed to investigate the potential of strategic palmar trimming before conventional shoeing for managing hoof imbalance in horses with underrun heels. Methods: Eleven affected horses were divided into control (5 horses with 14 defective hooves) and experimental (6 horses with 16 defective hooves) groups. The experimental group underwent strategic palmar trimming before conventional shoeing, while the control horses received regular trimming before traditional shoeing. Dorsal hoof wall angle (DHWA)/heel angle (HA) ratios, heel-bulb distance, and six parallel lines indicating solar surface dimension were determined before, and after 5, 10, 15, 20, 25, and 30 weeks of the different trimming protocols. Palmar trimming distance was only measured in experimental horses. Results: A group-by-time interaction was observed for DHWA/HA ratios and solar surface dimension. Separate effects for group and time were observed in the heel-bulb distance. A modification in hoof conformation was detected at 25-30 weeks only in horses in the experimental group, including decreases in DHWA/HA ratio, heel-bulb distance, and palmar trimming distance, coinciding with an increase in palmar width of trimmed hooves. Conclusions: Different trimming protocols produced a different effect on hoof conformation. Therefore, tactical remedial trimming can potentially control poor hoof conformation in horses with underrun heels.