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Dynamics of endocytic clathrin-coated structures can be remarkably divergent across different cell types, cells within the same culture, or even distinct surfaces of the same cell. The origin of this astounding heterogeneity remains to be elucidated. Here we show that cellular processes associated with changes in effective plasma membrane tension induce significant spatiotemporal alterations in endocytic clathrin coat dynamics. Spatiotemporal heterogeneity of clathrin coat dynamics is also observed during morphological changes taking place within developing multicellular organisms. These findings suggest that tension gradients can lead to patterning and differentiation of tissues through mechanoregulation of clathrin-mediated endocytosis.
Assuntos
Vesículas Revestidas por Clatrina/fisiologia , Animais , Fenômenos Biomecânicos , Linhagem Celular Tumoral , Membrana Celular/metabolismo , Membrana Celular/fisiologia , Células Cultivadas , Chlorocebus aethiops , Clatrina/metabolismo , Vesículas Revestidas por Clatrina/metabolismo , Invaginações Revestidas da Membrana Celular/metabolismo , Invaginações Revestidas da Membrana Celular/fisiologia , Citoplasma/metabolismo , Drosophila , Endocitose/fisiologia , Humanos , Análise Espaço-TemporalRESUMO
The request for stable and reliable devices is tremendous in the field of optogenetics. So far, no device which is called optrode, encapsulating the needed light source hermetically, can be found. We therefore introduce a novel optrode concept consisting of polyimide, silicone as well as a silicon- and fused silica-based hermetic package. One of the main features of the hermetic package is the integration of custom-made microlenses. These microlenses are fabricated using thermal reflow of photoresist. Chosen parameters for remelting the photoresist AZ9260 are 2 min @ 160 °C. An additional dry etching step is introduced to transfer the resist pattern into a fused silica substrate. We were able to fabricate lenses in diameters ranging from 25 µm to 1300 µm. The focal lengths of the etched lenses vary from 630 µm to 5500 µm for lens diameter ranging from 200 µm to 900 µm. Deviations of the transferred pattern to an ideal sphere range from 0.055 % and -0.151 % to 0.040 % and -0.003 % (300 µm and 700 µm lens diameter) and can be neglected.
Assuntos
Eletrodos Implantados , Optogenética/instrumentação , Dióxido de Silício/química , MicrotecnologiaRESUMO
The quest for realization of non-Abelian phases of matter, driven by their possible use in fault-tolerant topological quantum computing, has been spearheaded by recent developments in p-wave superconductors. The chiral p(x)+ip(y)-wave superconductor in two-dimensions exhibiting Majorana modes provides the simplest phase supporting non-Abelian quasiparticles and can be seen as the blueprint of fractional topological order. Alternatively, Kitaev's Majorana wire has emerged as an ideal toy model to understand Majorana modes. Here we present a way to make the transition from Kitaev's Majorana wires to two-dimensional p-wave superconductors in a system with cold atomic gases in an optical lattice. The main idea is based on an approach to generate p-wave interactions by coupling orbital degrees of freedom with strong s-wave interactions. We demonstrate how this design can induce Majorana modes at edge dislocations in the optical lattice, and we provide an experimentally feasible protocol for the observation of the non-Abelian statistics.
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We explore the nature of the Bose condensation transition in driven open quantum systems, such as exciton-polariton condensates. Using a functional renormalization group approach formulated in the Keldysh framework, we characterize the dynamical critical behavior that governs decoherence and an effective thermalization of the low frequency dynamics. We identify a critical exponent special to the driven system, showing that it defines a new dynamical universality class. Hence critical points in driven systems lie beyond the standard classification of equilibrium dynamical phase transitions. We show how the new critical exponent can be probed in experiments with driven cold atomic systems and exciton-polariton condensates.
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We present a method to design a finite decay rate for excited rotational states in polar molecules. The setup is based on a hybrid system of polar molecules with atoms driven into a Rydberg state. The atoms and molecules are coupled via the strong dipolar exchange interaction between two rotation levels of the polar molecule and two Rydberg states. Such a controllable decay rate opens the way to optically pump the hyperfine levels of polar molecules and it enables the application of conventional laser cooling techniques for cooling polar molecules into quantum degeneracy.
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We investigate experimentally and theoretically the dynamical properties of a Mott insulator in decoupled one-dimensional chains. Using a theoretical analysis of the Bragg excitation scheme, we show that the spectrum of interband transitions holds information on the single-particle Green's function of the insulator. In particular, the existence of particle-hole coherence due to quantum fluctuations in the Mott state is clearly seen in the Bragg spectra and quantified. Finally, we propose a scheme to directly measure the full, momentum-resolved spectral function as obtained in the angle-resolved photoemission spectroscopy of solids.
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The experimental investigation of quantum phases in optical lattice systems provides major challenges. Recently, dynamical generation of double occupancy via modulation of the hopping amplitude t has been used to characterize the strongly correlated phase of fermionic atoms. Here, we want to validate this experimental technique with a theoretical study of the driven Hubbard model using analytic methods. We find that conclusive evidence for a Mott phase can be inferred from such a measurement, provided that sufficiently low temperatures k_{B}T<
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We derive the collective low-energy excitations of the quantum phase model of interacting lattice bosons within the superfluid state using a dynamical variational approach. We recover the well-known sound (or Goldstone) mode and derive a gapped (Higgs-type) mode that was overlooked in previous studies of the quantum phase model. This mode is relevant to ultracold atoms in a strong optical lattice potential. We predict the signature of the gapped mode in lattice modulation experiments and show how it evolves with increasing interaction strength.
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We present the design of a ring exchange interaction in cold atomic gases subjected to an optical lattice using well-understood tools for manipulating and controlling such gases. The strength of this interaction can be tuned independently and describes the correlated hopping of two bosons. We discuss a setup where this coupling term may allow for the realization and observation of exotic quantum phases, including a deconfined insulator described by the Coulomb phase of a three-dimensional U(1) lattice gauge theory.