RESUMO
INTRODUCTION: Little is known about how the amplitude and timing of transmembrane current pulses affect transmembrane potential (Vm) and action potential duration (APD) in isolated myocytes. METHODS AND RESULTS: Ten ventricular myocytes were isolated from five rabbit hearts. Each cell was paced at an S1 cycle length of 250 msec, and S2 pulses of 10-msec duration were delivered at various strengths and time intervals. For all S2 strengths (0.2 to 1.5 nA), the magnitude of changes in Vm did not depend on polarity during the plateau, but were larger for depolarizing pulses during phase 3 repolarization. However, the magnitude of changes in APD varied with polarity during the entire action potential for strengths ranging from 0.5 to 1.5 nA. Greater changes in APD occurred for hyperpolarizing pulses during the plateau and depolarizing pulses during phase 3. In addition, we used a cardiac phase variable to quantify the current threshold for regenerative depolarization and repolarization as a function of prestimulus Vm. Regenerative depolarization occurred during phase 3 repolarization, and its current threshold was less than that required for regenerative repolarization that occurred during the plateau. These data were compared to computer simulations in a patch of membrane represented by Luo-Rudy dynamic kinetics, and the results were qualitatively similar, including the higher threshold for regenerative repolarization compared to regenerative depolarization. CONCLUSION: This characterization of the nonlinear response of isolated cells to transmembrane current, including phase resetting, should aid in understanding the mechanisms of defibrillation because shock-induced changes in Vm and APD have been implicated as important factors in determining defibrillation success.
Assuntos
Potenciais de Ação/fisiologia , Miocárdio/citologia , Pulso Arterial , Função Ventricular , Animais , Comunicação Celular/fisiologia , Simulação por Computador , Ventrículos do Coração/citologia , Modelos Animais , Modelos Cardiovasculares , Coelhos , Tempo de Reação/fisiologia , Limiar Sensorial/fisiologiaRESUMO
OBJECTIVE: While several studies have demonstrated that the L-type calcium current maintains discontinuous conduction, the contribution of the transient outward current (I(to)) to conduction remains unclear. This study evaluated the effects of I(to) inhibition on conduction between ventricular myocytes. METHODS: An electronic circuit with a variable resistance (R(j)) was used to electrically couple single epicardial myocytes isolated from rabbit right ventricle. We inhibited I(to) with 4-aminopyridine superfusion, rate-acceleration, or premature stimulation to evaluate the subsequent effects on conduction delay and the critical R(j), which was quantified as the highest R(j) that could be imposed before conduction failed. RESULTS: I(to) inhibition significantly enhanced conduction in all cell pairs (n=23). Pharmacologic inhibition of I(to) resulted in a 32+/-5% decrease in conduction delay and a 36+/-7% increase in critical R(j). Similarly, reduction of the basic cycle length from 2 to 0.5 s resulted in a 31+/-3% decrease in conduction delay and a 31+/-3% increase in critical R(j). Finally, premature action potentials conducted with a 41+/-4% shorter conduction delay and a 73+/-24% higher critical R(j) than basic action potentials. CONCLUSIONS: I(to) inhibition significantly enhanced conduction across high R(j). These results suggest I(to) may contribute to rate-dependent conduction abnormalities.
Assuntos
4-Aminopiridina/farmacologia , Arritmias Cardíacas/metabolismo , Ventrículos do Coração/metabolismo , Canais de Potássio/efeitos dos fármacos , Potenciais de Ação/efeitos dos fármacos , Animais , Comunicação Celular , Separação Celular/métodos , Células Cultivadas , Estimulação Elétrica , Potenciais da Membrana/efeitos dos fármacos , CoelhosRESUMO
Many studies suggest that early afterdepolarizations (EADs) arising from Purkinje fibers initiate triggered arrhythmias under pathological conditions. However, electrotonic interactions between Purkinje and ventricular myocytes may either facilitate or suppress EAD formation at the Purkinje-ventricular interface. To determine conditions that facilitated or suppressed EADs during Purkinje-ventricular interactions, we coupled single Purkinje myocytes and aggregates isolated from rabbit hearts to a passive model cell via an electronic circuit with junctional resistance (R(j)). The model cell had input resistance (R(m,v)) of 50 M Omega, capacitance of 39 pF, and a variable rest potential (V(rest,v)). EADs were induced in Purkinje myocytes during superfusion with 1 microM isoproterenol. Coupling at high R(j) to normally polarized V(rest,v) established a repolarizing coupling current during all phases of the Purkinje action potential. This coupling current preferentially suppressed EADs in single cells with mean membrane resistance (R(m,p)) of 297 M Omega, whereas EAD suppression in larger aggregates with mean R(m,p) of 80 M Omega required larger coupling currents. In contrast, coupling to elevated V(rest,v) established a depolarizing coupling current during late phase 2, phase 3, and phase 4 that facilitated EAD formation and induced spontaneous activity in single Purkinje myocytes and aggregates. These results have important implications for arrhythmogenesis in the infarcted heart when reduction of the ventricular mass due to scarring alters the R(m,p)-to-R(m,v) ratio and in the ischemic heart when injury currents are established during coupling between polarized Purkinje myocytes and depolarized ventricular myocytes.
Assuntos
Coração/fisiologia , Ramos Subendocárdicos/fisiologia , Função Ventricular/fisiologia , Animais , Células Cultivadas , Ventrículos do Coração , Técnicas In Vitro , Potenciais da Membrana , Técnicas de Patch-Clamp , CoelhosRESUMO
Purkinje-ventricular junctions (PVJs) have been implicated as potential sites of arrhythmogenesis, in part because of the dispersion of action potential duration (APD) between Purkinje (P) and ventricular (V) myocytes. To characterize electrotonic modulation of APD as a function of junctional resistance (Rj), we coupled single isolated rabbit P and V myocytes with an electronic circuit. In seven of eight PV myocyte pairs, both APDs shortened on coupling at Rj = 50 MOmega. This was in contrast to modulation of APD in paired ventricular myocytes, which demonstrated APD shortening of the intrinsically longer action potential and APD prolongation of the intrinsically shorter action potential. Companion computer simulations, performed to suggest possible mechanisms for the paradoxical shortening of the V action potential in paired P and V myocytes, showed that the difference in intrinsic peak plateau potentials (Vpp) of the P and V myocytes determined whether the V action potential shortened or prolonged on coupling. This difference in Vpp caused a large, repolarizing coupling current to flow to the V myocyte, contributing to early inactivation of the L-type calcium current and early activation of the inward rectifier current. These results suggest that intrinsic differences in phase 1 repolarization could yield differing patterns of APD shortening or prolongation in the network of subendocardial PVJs, leaving some PVJs vulnerable to conduction of premature stimuli while other PVJs remain refractory.
Assuntos
Comunicação Celular/fisiologia , Ramos Subendocárdicos/fisiologia , Função Ventricular/fisiologia , 4-Aminopiridina/farmacologia , Potenciais de Ação/efeitos dos fármacos , Potenciais de Ação/fisiologia , Animais , Simulação por Computador , Impedância Elétrica , Eletrofisiologia , Modelos Cardiovasculares , Miocárdio/citologia , Ramos Subendocárdicos/citologia , Coelhos , Tempo de Reação/efeitos dos fármacos , Tempo de Reação/fisiologiaRESUMO
Conduction at the Purkinje-ventricular junction (PVJ) demonstrates unidirectional block under both physiological and pathophysiological conditions. Although this block is typically attributed to multidimensional electrotonic interactions, we examined possible membrane-level contributions using single, isolated rabbit Purkinje (P) and ventricular (V) myocytes coupled by an electronic circuit. When we varied the junctional resistance (Rj) between paired V myocytes, conduction block occurred at lower Rj values during conduction from the smaller to larger myocyte (115 +/- 59 M omega) than from the larger to smaller myocyte (201 +/- 51 M omega). In Purkinje-ventricular myocyte pairs, however, block occurred at lower Rj values during P-to-V conduction (85 +/- 39 M omega) than during V-to-P conduction (912 +/- 175 M omega), although there was little difference in the mean cell size. Companion computer simulations, performed to examine how the early platea currents affected conduction, showed that P-to-V block occurred at lower Rj values when the transient outward current was increased or the calcium current was decreased in the model P cell. These results suggest that intrinsic differences in phase 1 repolarization can contribute to unidirectional block at the PVJ.