RESUMO
Flavonol 2,4-dioxygenase, which catalyzes the cleavage of quercetin to carbon monoxide and 2-protocatechuoyl-phloroglucinol carboxylic acid, was purified from culture filtrate of Aspergillus niger DSM 821 grown on rutin. It is a glycoprotein (46-54% carbohydrate) with N-linked oligo-mannose type glycan chains. The enzyme was resolved in SDS polyacrylamide gels in a diffuse protein band that corresponded to a molecular mass of 130-170 kDa. When purified flavonol 2,4-dioxygenase was heated, it dissociated into three peptides with apparent molecular masses of 63-67 kDa (L), 53-57 kDa (M), and 31-35 kDa (S), which occurred in a molar ratio of 1:1:1, suggesting a LMS structure. Crosslinking led to a 90-97 kDa species, concomitant with the decrease of staining intensity of the 63-67 kDa (L) and the 31-35 kDa (S) peptides. Analysis by matrix-assisted laser desorption/ionization-time of flight-MS showed peaks at m/z approximately 69 600, m/z approximately 51 700, and m/z approximately 26 500 which are presumed to represent the three peptides of flavonol 2,4-dioxygenase, and a broad peak at m/z approximately 96 300, which might correspond to the LS heterodimer as formed in the crosslinking reaction. Based on the estimated molecular mass of 148 kDa, 1 mol of enzyme contained 1.0-1.6 mol of copper. Ethylxanthate, which specifically reduces CuII to CuI ethylxanthate, is a potent inhibitor of flavonol 2,4-dioxygenase. Metal chelating agents (such as diethyldithiocarbamate, diphenylthiocarbazone) strongly inhibited the enzymatic activity, but inactivation was not accompanied by loss of copper. The EPR spectrum of flavonol 2,4-dioxygenase (as isolated) showed the characteristic parameters of a nonblue type 2 CuII protein. The Cu2+ is assumed to interact with four nitrogen ligands, and the CuII complex has a (distorted) square planar geometry.
Assuntos
Aspergillus niger/enzimologia , Cobre/análise , Dioxigenases , Glicoproteínas/química , Glicoproteínas/metabolismo , Oxigenases/química , Oxigenases/metabolismo , Aspergillus niger/crescimento & desenvolvimento , Cromatografia de Afinidade , Cromatografia por Troca Iônica , Reagentes de Ligações Cruzadas , Espectroscopia de Ressonância de Spin Eletrônica , Glicoproteínas/isolamento & purificação , Substâncias Macromoleculares , Peso Molecular , Oxigenases/isolamento & purificação , Rutina/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Especificidade por Substrato , UltrafiltraçãoRESUMO
Quinaldine catabolism was investigated with the bacterial strain Arthrobacter sp., which is able to grow aerobically in a mineral salt medium with quinaldine as sole source of carbon, nitrogen and energy. The following degradation products of quinaldine were isolated from the culture fluid and identified: 1H-4-oxoquinaldine, N-acetylisatic acid, N-acetylanthranilic acid, anthranilic acid, 3-hydroxy-N-acetylanthranilic acid and catechol. 3-Hydroxy-N-acetylanthranilic acid was not further metabolized by this organism. A degradation pathway is proposed.
Assuntos
Arthrobacter/metabolismo , Quinaldinas/metabolismo , Quinolinas/metabolismo , Catecóis/metabolismo , Fenilacetatos/metabolismo , ortoaminobenzoatos/metabolismoRESUMO
Actinoplanes missouriensis utilizes arogenate as an intermediate in L-tyrosine biosynthesis, while no evidence of prephenate dehydrogenase was observed. Arogenate dehydrogenase has been partially purified by a five-step procedure. The enzyme requires NAD as cofactor. The Km values for NAD and arogenate are 0.2 mM and 0.15 mM, respectively. The molecular weight of arogenate dehydrogenase is about 68,000, and SDS gel electrophoresis indicates a composition of two identical subunits. The enzyme is not feedback inhibited by L-tyrosine and unaffected by L-phenylalanine, prephenate, phenylpyruvate, p-hydroxyphenylpyruvate or L-tryptophan. Arogenate dehydrogenase is quite sensitive to p-hydroxymercuribenzoate with 50% inhibition at 12.5 microM of the SH-specific reagent. The presence of malate in usually applied arogenate preparations is demonstrated and the consequence of an impure substrate on arogenate dehydrogenase studies is discussed.
Assuntos
Actinomycetales/enzimologia , Oxirredutases/isolamento & purificação , Prefenato Desidrogenase , Cromatografia DEAE-Celulose , Cromatografia por Troca Iônica , Cinética , Peso Molecular , Oxirredutases/metabolismoRESUMO
Malate dehydrogenases from Streptosporangium roseum (DSM 43021), Planomonospora venezuelensis (DSM 43178), Microtetraspora glauca (ATCC 23057), Actinoplanes missouriensis (DSM 43046), Streptomyces atratus (ATCC 14046), Kibdelosporangium aridum (ATCC 39323), and from Phenylobacterium immobile, strain E (DSM 1986) were purified to homogeneity. The N-terminal amino-acid sequences were determined and compared with known prokaryotic and eukaryotic sequence data. The partial sequences from Actinomycetales enzymes include a string of amino acids which is also present in the N-terminal region of malate dehydrogenases from Thermus flavus and from mammalian cytoplasm.
Assuntos
Actinomycetales/enzimologia , Bactérias Gram-Negativas/enzimologia , Malato Desidrogenase/análise , Sequência de Aminoácidos , Sulfato de Amônio , Cromatografia de Afinidade , Cromatografia DEAE-Celulose , Cromatografia por Troca Iônica , Meios de Cultura , Eletroforese em Gel de Poliacrilamida , Malato Desidrogenase/isolamento & purificação , Dados de Sequência MolecularRESUMO
The enzymes of the terminal steps of the phenylalanine and tyrosine biosynthesis were partially purified and characterized in Microtetraspora glauca, a spore-forming member of the order Actinomycetales. This bacterium relies exclusively on the phenylpyruvate route for phenylalanine synthesis, no arogenate dehydratase activity being found. Prephenate dehydratase is subject to feedback inhibition by phenylalanine, tyrosine and tryptophan, each acting as competitive inhibitor by increasing the Km of 72 microM for prephenate. Based on the results of gel chromatography on Sephadex G-200, the molecular mass of about 110,000 Da is not altered by any of the effectors. The enzyme is quite sensitive to inhibition by 4-hydroxymercuribenzoate. Microtetraspora glauca can utilize arogenate and 4-hydroxyphenylpyruvate as intermediates in tyrosine biosynthesis. Prephenate and arogenate dehydrogenase activities copurifying from ion exchange columns with coincident profiles were detected. From gel-filtration columns the two activities eluted at an identical molecular-mass position of about 68,000 Da. The existence of a single protein exhibiting substrate ambiguity is consistent with the findings, that both dehydrogenases have similar chromatographic properties, exhibit cofactor requirement for NAD and are inhibited to the same extent by tyrosine and 4-hydroxymercuribenzoate.