An enzyme free simultaneous detection of γ-amino-butyric acid and testosterone based on copper oxide nanoparticles.

Herein, an easy wet-chemical process was used in basic medium with low temperature to prepare low-dimensional copper oxide nanoparticles (CuO NPs). A variety of optical and structural techniques such as UV-visible, FT-IR, XRD, FESEM, XEDS, and XPS were used to characterize the synthesized CuO NPs in detail. Two sensitive and selective sensor probes for γ-amino-butyric acid (GABA) and testosterone (TST) were achieved after modification; a thin layer of NPs on a flat glassy carbon electrode (GCE). Sensor analytical parameters such as sensitivity (SNT), linear dynamic range (LDR), limit of detection (LOD), limit of quantification (LOQ), robustness, and interference effects, were evaluated for the proposed sensor (GCE/CuO NPs) for GABA and TST, based on a dependable current-voltage technique. Calibration curves were found to be linear (R 2 = 0.9963 and 0.9095) over a broad concentration range of GABA and TST (100.0 pM to 100.0 mM and 10.0 pM to 10.0 mM, respectively). Sensor parameters - SNT (316.46 and 2848.10 pA µM-1 cm-2), LDR (100.0 nM to 10.0 mM and 10.0 pM to 1.0 mM), LOD (≈11.70 and 96.67 pM), and LOQ (39.0 and 322.2 pM) - for GABA and TST were calculated from the calibration plot successively. Preparation of CuO NPs using the wet-chemical technique is a good approach for perspective expansion of NPs-based sensors for the enzyme-free detection of biomolecules. Our sensor probe (GCE/CuO NPs) is applied for the cautious recognition of GABA and TST in real biological samples -human, mouse, and rabbit serum - and achieved good and acceptable results.

The synthesis and application of (E)-N'-(benzo[d]dioxol-5-ylmethylene)-4-methyl-benzenesulfonohydrazide for the detection of carcinogenic lead.

In this study, noble ligands of (E)-N'-(benzo[d]dioxol-5-ylmethylene)-4-methyl-benzenesulfonohydrazide (BDMMBSH) were prepared via a simple condensation method using benzo-[d][1,3]-dioxole carbaldehyde, benzenesulfonylhydrazine (BSH), and 4-methyl-benzenesulphonylhydrazine (4-MBSH) in good yield, which were crystallized in acetone, EtOAc, and EtOH. The BDMMBSH derivatives were characterized using different spectroscopic techniques, such as 1H-NMR, 13C-NMR, FTIR, and UV-Vis spectroscopy, and their crystal structures were analyzed using the single crystal X-ray diffraction method (SCXRDM). Subsequently, the BDMMBSH compounds were used for the significant detection of the carcinogenic heavy metal ion, lead (Pb2+), via a reliable electrochemical approach. A sensitive and selective Pb2+ sensor was developed via the deposition of a thin layer of BDMMBSH on a GCE with the conducting polymer matrix Nafion (NF). The sensitivity, LOQ, and LOD of the proposed sensor towards Pb2+ were calculated from the calibration curves to be 2220.0 pA µM-1 cm-2, 320.0 mM, and 96.0 pM, respectively. The validation of the BDMMBSH/GCE/NF sensor probe was performed via the selective determination of Pb2+ in spiked natural samples with a satisfactory and rational outcome.

Simultaneous detection of l-aspartic acid and glycine using wet-chemically prepared Fe3O4@ZnO nanoparticles: real sample analysis.

An easy and reliable wet-chemical method was used to synthesize iron oxide doped zinc oxide nanoparticles (Fe3O4@ZnO NPs) at a low-temperature under alkaline medium. The electrochemical characteristics of Fe3O4@ZnO NPs were investigated by using different electrochemical techniques such as UV-vis, FTIR, XRD, FESEM, XEDS, and XPS. A sensor was fabricated by deposition of a thin covering of Fe3O4@ZnO NPs onto a flat dried glassy carbon electrode (GCE) with a polymer matrix with conducting characteristics (Nafion, Nf). l-Aspartic acid and glycine were detected simultaneously by using the modified GCE/Fe3O4@ZnO NPs/Nf sensor in enzyme free conditions. Calibration curves were found to be linear for l-aspartic acid (R 2 = 0.9593) and glycine (R 2 = 0.8617) over a broad range of detected bio-molecule concentration (100.0 pM to 100.0 mM). The analytical sensing parameters, for example sensitivity, linear dynamic range (LDR), limit of detection (LOD), and limit of quantification (LOQ), of the proposed sensor (GCE/Fe3O4@ZnO NPs/Nf) were calculated at two potentials (+0.4 V and +0.7 V) from the calibration plot for l-aspartic acid (126.58 pM µM-1 cm2, 100.0 pM to 10.0 µM, ≈97.5 pM, and 325.0 mM) and glycine (316.46 pM µM-1 cm2, 1.0 µM to 1.0 mM, ≈13.5 pM, and 450.0 mM), respectively, by using a reliable current-voltage (I-V) technique. The synthesis of Fe3O4@ZnO NPs by means of a wet-chemical route is a good advancement for the development of doped nanomaterial based sensors from the perspective of enzyme-free detection of biological molecules in health-care areas. This proposed GCE/Fe3O4@ZnO NPs/Nf sensor was used for the particular detection of l-aspartic acid and glycine in real samples (human and rabbit serum and urine) and found to achieve reasonable and accepted results.

d-Glucose sensor based on ZnO·V2O5 NRs by an enzyme-free electrochemical approach.

A simple wet-chemical technique was used to prepare zinc oxide-doped vanadium pentaoxide nanorods (ZnO·V2O5 NRs) in an alkaline environment. The synthesized ZnO·V2O5 NRs were characterized using typical methods, including UV-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (XEDS), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD). The d-glucose (d-GLC) sensor was fabricated with modification of a slight coating of nanorods (NRs) onto a flat glassy carbon electrode (GCE). The analytical performances, such as the sensitivity, limit of quantification (LOQ), limit of detection (LOD), linear dynamic range (LDR), and durability, of the proposed d-GLC sensor were acquired by a dependable current-voltage (I-V) process. A calibration curve of the GCE/ZnO·V2O5 NRs/Nf sensor was plotted at +1.0 V over a broad range of d-GLC concentrations (100.0 pM-100.0 mM) and found to be linear (R 2 = 0.6974). The sensitivity (1.27 × 10-3 µA µM-1 cm-2), LOQ (417.5 mM), and LOD (125 250 µM) were calculated from the calibration curve. The LDR (1.0 µM-1000 µM) was derived from the calibration plot and was also found to be linear (R 2 = 0.9492). The preparation of ZnO·V2O5 NRs by a wet-chemical technique is a good advancement for the expansion of nanomaterial-based sensors to support enzyme-free sensing of biomolecules in healthcare fields. This fabricated GCE/ZnO·V2O5 NRs/Nf sensor was used for the recognition of d-glucose in real samples (apple juice, human serum, and urine) and returned satisfactory and rational outcomes.

Fabrication of 3-methoxyphenol sensor based on Fe3O4 decorated carbon nanotube nanocomposites for environmental safety: Real sample analyses.

Iron oxide ornamented carbon nanotube nanocomposites (Fe3O4.CNT NCs) were prepared by a wet-chemical process in basic means. The optical, morphological, and structural characterizations of Fe3O4.CNT NCs were performed using FTIR, UV/Vis., FESEM, TEM; XEDS, XPS, and XRD respectively. Flat GCE had been fabricated with a thin-layer of NCs using a coating binding agent. It was performed for the chemical sensor development by a dependable I-V technique. Among all interfering analytes, 3-methoxyphenol (3-MP) was selective towards the fabricated sensor. Increased electrochemical performances for example elevated sensitivity, linear dynamic range (LDR) and continuing steadiness towards selective 3-MP had been observed with chemical sensor. The calibration graph found linear (R2 = 0.9340) in a wide range of 3-MP concentration (90.0 pM ~ 90.0 mM). The limit of detection and sensitivity were considered as 1.0 pM and 9×10-4 µAµM-1cm-2 respectively. The prepared of Fe3O4.CNT NCs by a wet-chemical progression is an interesting route for the development of hazardous phenolic sensor based on nanocomposite materials. It is also recommended that 3-MP sensor is exhibited a promising performances based on Fe3O4.CNT NCs by a facile I-V method for the significant applications of toxic chemicals for the safety of environmental and health-care fields.

Trivalent Y3+ ionic sensor development based on (E)-Methyl-N'-nitrobenzylidene-benzenesulfonohydrazide (MNBBSH) derivatives modified with nafion matrix.

(E)-Methyl-N'-nitrobenzylidene-benzenesulfonohydrazide (MNBBSH) compounds were synthesized using a condensation procedure from the derivatives of nitrobenzaldehyde and 4-Methyl-benzenesulfonylhydrazine, which crystallized in ethanol and methanol as well as characterized by FTIR, UV-Vis, 1H-NMR, and 13C-NMR. MNBBSH structure was confirmed using a single crystal X-ray diffraction technique and used for the detection of selective yttrium ion (Y3+) by I-V system. A thin layer of MNBBSH was deposited onto a glassy carbon electrode (GCE) with 5% nafion for the sensitive and selective Y3+ sensor. The modified MNBBSH/GCE sensor is exhibited the better electrochemical performances such as sensitivity, limit of detection (LOD), linear dynamic range (LDR), limit of quantification (LOQ), short response time, and long term storage ability towards the selective metal ion (Y3+). The calibration curve of 2-MNBBSH/GCE sensor was plotted at +1.1 V over a broad range of Y3+ concentration. Sensitivity, LOD, LDR and LOQ of the fabricated sensor towards Y3+ were calculated from the calibration curve and found as 1.90 pAµM-1 cm-2, 10.0 pM, 1.0 nM~1.0 mM and 338.33 mM respectively. The 2-MNBBSH/Nafion/GCE sensor was applied to the selective determination of Y3+ in spiked samples such as industrial effluent and real water samples from different sources, and found acceptable and reasonable results.

Ultrasensitive and selective 4-aminophenol chemical sensor development based on nickel oxide nanoparticles decorated carbon nanotube nanocomposites for green environment.

Nickel oxide nanoparticles decorated carbon nanotube nanocomposites (NiO·CNT NCs) were prepared in a basic medium by using facile wet-chemical routes. The optical, morphological, and structural properties of NiO·CNT NCs were characterized using Fourier transformed infra-red (FT-IR), Ultra-violet visible (UV/Vis) spectroscopy, field-emission scanning electron microscopy (FESEM), X-ray energy dispersed spectroscopy (XEDS), X-ray photoelectron spectroscopy (XPS), and powder X-ray diffraction (XRD) methods. Selective 4-aminophenol (4-AP) chemical sensor was developed by a flat glassy carbon electrode (GCE, surface area: 0.0316cm2) fabricated with a thin-layer of NCs. Electrochemical responses including higher sensitivity, large dynamic range (LDR), limit of detection (LOD), and long-term stability towards 4-AP were obtained using the fabricated chemical sensors. The calibration curve was found linear (R2=0.914) over a wide range of 4-AP concentration (0.1nmol/L-0.1mol/L). In perspective of slope (2×10-5µA/µM), LOD and sensitivity were calculated as 15.0±0.1pM and ~6.33×10-4µA/(µM·cm) respectively. The synthesized NiO·CNT NCs using a wet-chemical method is a significant route for the development of ultrasensitive and selective phenolic sensor based on nano-materials for environmental toxic substances. It is suggested that a pioneer and selective development of 4-AP sensitive sensor using NiO·CNT NCs by a facile and reliable current vs voltage (I-V) method for the major application of toxic agents in biological, green environmental, and health-care fields in near future.

Influence of chain length on the activity of tripeptidomimetic antagonists for CXC chemokine receptor 4 (CXCR4).

Here we report a series of close analogues of our recently published scaffold-based tripeptidomimetic CXCR4 antagonists, containing positively charged guanidino groups in R1 and R2, and an aromatic group in R3. While contraction/elongation of the guanidine carrying side chains (R1 and R2) resulted in loss of activity, introduction of bromine in position 1 on the naphth-2-ylmethyl moiety (R3) resulted in an EC50 of 61µM (mixture of diastereoisomers) against wild-type CXCR4; thus, the antagonistic activity of these tripeptidomimetics seems to be amenable to optimization of the aromatic moiety. Moreover, for analogues carrying a naphth-2-ylmethyl substituent, we observed that a Pictet-Spengler like cyclization side reaction depended on the nature of the R1 substituent.