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Leukemia ; 31(11): 2315-2325, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28216661

RESUMO

Deregulation of epigenetic mechanisms, including microRNA, contributes to leukemogenesis and drug resistance by interfering with cancer-specific molecular pathways. Here, we show that the balance between miR-194-5p and its newly discovered target BCL2-associated transcription factor 1 (BCLAF1) regulates differentiation and survival of normal hematopoietic progenitors. In acute myeloid leukemias this balance is perturbed, locking cells into an immature, potentially 'immortal' state. Enhanced expression of miR-194-5p by treatment with the histone deacetylase inhibitor SAHA or by exogenous miR-194-5p expression re-sensitizes cells to differentiation and apoptosis by inducing BCLAF1 to shuttle between nucleus and cytosol. miR-194-5p/BCLAF1 balance was found commonly deregulated in 60 primary acute myeloid leukemia patients and was largely restored by ex vivo SAHA treatment. Our findings link treatment responsiveness to re-instatement of miR-194-5p/BCLAF1 balance.


Assuntos
Regulação da Expressão Gênica , Leucemia Mieloide Aguda/patologia , MicroRNAs/genética , Proteínas Repressoras/genética , Proteínas Supressoras de Tumor/genética , Apoptose , Ciclo Celular , Diferenciação Celular , Linhagem Celular Tumoral , Regulação para Baixo , Humanos , Leucemia Mieloide Aguda/genética
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