Development and validation of an effective and sensitive technique for nitrate determination in fruits and vegetables using HPLC/PDA.

This study aims to develop an effective and sensitive HPLC (High Performance Liquid Chromatography) method to determine the nitrate concentration in fruits and vegetables (F & V) using a C18 column (ZORBAX Eclipse XDB-C18, 80Å, 250 × 4.6 mm, 5 µm (Agilent Technologies)) maintained at 40 0 C, a mobile phase made up of methanol and buffer (pentane sulfonic acid sodium salt solution), and a Photo Diode Array Detector (PDA) at 225 nm. The developed method is validated in terms of selectivity, linearity, accuracy, precision, suitability, the limit of detection (LOD), and the limit of quantification (LOQ) according to the European Union Decision 2002/657/EC. The result revealed that a ratio of 30: 70 of the organic modifier methanol and buffer with pH 2.8 shows the highest efficiency. The calibration curve shows linearity with a correlation coefficient (r) of 0.9985. The LOD and LOQ were found to be 2.26 mg/kg and 7.46 mg/kg. The recovery was in the range of 98.96-100.21%. Moreover, the greenness assessment scores of different approaches (eco-scale score of 76, AGREE score of 0.71, and few red shades in GAPI portray) were at a very excellent level. Thus, our developed method is fully validated and can determine the nitrate content in F & V.

Effect of bismuth doping on the crystal structure and photocatalytic activity of titanium oxide.

The doping of TiO2 with metals and non-metals is considered one of the most significant approaches to improve its photocatalytic efficiency. In this study, the photodegradation of methyl orange (MO) was examined in relation to the impact of Bi-doping of TiO2. The doped TiO2 with various concentrations of metal was successfully synthesized by a one-step hydrothermal method and characterized using X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM), and UV-vis spectroscopy. The XRD results revealed that the anatase phase, with an average crystallite size of 16.2 nm, was the main phase of TiO2. According to the anatase texture results, it was found that the doping of TiO2 increased the specific surface area for Bi2O3@TiO2 without a change in the crystal structure or the crystal phase of TiO2. Also, XPS analysis confirmed the formation of Ti4+ and Ti3+ as a result of doping with Bi. The activities of both pure TiO2 and Bi-doped TiO2 were tested to study their ability to decolorize MO dye in an aqueous solution. The photocatalytic degradation of MO over Bi2O3@TiO2 reached 98.21%, which was much higher than the 42% achieved by pure TiO2. Doping TiO2 with Bi increased its visible-light absorption as Bi-doping generated a new intermediate energy level below the CB edge of the TiO2 orbitals, causing a shift in the band gap from the UV to the visible region, thus enhancing its photocatalytic efficiency. In addition, the effects of the initial pH, initial pollutant concentration, and contact time were examined and discussed.

Is women's household decision-making autonomy associated with their higher dietary diversity in Bangladesh? Evidence from nationally representative survey.

In Bangladesh, a low-quality repetitive diet characterized by starchy staple foods is typical, leading to disorders associated with micronutrient deficiencies, particularly among mothers and their children. The purpose of the study was to validate the link between women's decision-making autonomy and higher dietary diversity score. Participants were ever married women aged 15-49 years old with comprehensive dietary information (n = 17,842), selected from the Bangladesh Demographic and Health Survey, 2014. The dietary diversity score (DDS) was obtained from a 24-h recall of dietary intake from nine food groups, categorized into lower DDS (DDS ≤ 4) and higher DDS (DDS ≥ 5). Descriptive analysis, bivariate and multivariate logistic regression were conducted using STATA version 15. Almost all women consumed starchy foods, flesh (83.86%), and fruits (67.30%). Using logistic regression, the odds of achieving dietary diversity score were higher among women who participated in household purchases (OR 2.40; 95% CI: 1.52-3.83; p = 0.022). Women who had higher and secondary education were 2.72 (95% CI: 1.49-3.02; p = 0.025) and 1.31 (95% CI: 0.58-2.18; p = 0.029) times more likely to achieve higher DDS than women having no education, as well as women in the richest quintile (OR 6.49; 95% CI: 4.12-8.5; p = 0.037) compared to women in the lowest quintile. This study highlighted the association of several socioeconomic conditions of ever married women and their dietary diversity score in Bangladesh. Therefore, promoting the women's education status, improving the socioeconomic conditions, and prioritizing their decisions are recommended for the attainment of higher dietary diversity score.

Optimizing gamma radiation shielding with cobalt-titania hybrid nanomaterials.

Cobalt-doped titania nanocomposites were fabricated to be utilized for radiation shielding aims. The chemical composition of the composites was measured using the energy-dispersive X-ray spectrometer. Moreover, the structure of the composites was evaluated using the X-ray diffractometer, and the morphology of the fabricated composites was presented using the scanning electron microscope. Furthermore, the γ-ray shielding properties were estimated using the Monte Carlo simulation between 0.059 and 2.506 MeV. The linear attenuation coefficient of the fabricated composites decreased by factors of 93% for all samples by raising the incident γ-energy between 0.059 and 2.506 MeV. Moreover, the partial replacement of the Ti4+ by Co3+ slightly enhanced the linear attenuation coefficient from 0.607 to 0.630 cm-1 when the Co3+ increased from 0 to 3.7 wt%. The improvement in the linear attenuation coefficient causes an enhancement in other radiation shielding properties.

Cobalt doped titania-carbon nanosheets with induced oxygen vacancies for photocatalytic degradation of uranium complexes in radioactive wastes.

The photocatalytic degradation of uranium complexes is considered among the most efficient techniques for the efficient removal of uranium ions/complexes from radioactive wastewater. Described here is a nanostructured photocatalyst based on a cobalt-doped TiO2 composite with induced oxygen vacancies (Co@TiO2-C) for the photocatalytic removal of uranium complexes from contaminated water. The synergy between oxygen vacancies and Co-doping produced a material with a 1.7 eV bandgap, while the carbon network facilitates electron movement and hinders the e-h recombination. As a result, the new photocatalyst enables the decomposition of uranium-arsenazo iii complexes (U-ARZ3), followed by photocatalytic reduction of hexavalent uranium to insoluble tetravalent uranium. Combined with the nanosheet structure's high surface area, the photocatalytic decomposition, reduction efficiency, and kinetics were significantly enhanced, achieving almost complete U(vi) removal in less than 20 minutes from solution with a concentration as high as 1000 mL g-1. Moreover, the designed photocatalyst exhibits excellent stability and reusability without decreasing the photocatalytic performance after 5 cycles.

Prevalence of childhood anemia: Potential sociodemographic and dietary factors in Nigeria.

BACKGROUND: Childhood anemia is a global public health issue. In this study, we assessed the potential sociodemographic and dietary factors associated with the prevalence of anemia among children aged 6-59 months in Nigeria. METHODS: In this cross-sectional study, we collected dietary information and demographic data on 6,338 children with anemia from the Nigerian Demographic and Health Surveys (2018). The association between the occurrence of anemia and the demographic and dietary factors was determined by conducting Chi-squared tests. Additionally, bivariate and multivariate order logit models were constructed and reported as odds ratios. RESULTS: The results of the multivariate analysis showed that the risk of anemia was reduced by 13% and 44% in children aged 13-36 months (OR = 0.87; 95% CI = 0.77-0.98; p = 0.019) and 37-59 months (OR = 0.56; 95% CI = 0.49-0.63; p < 0.001), respectively, compared to the risk of anemia in children aged 6-12 months. Anemia was 28% less likely in children of non-anemic mothers (OR = 0.72; 95% CI = 0.66-0.80; p < 0.001) than children of anemic mothers. Children fed pumpkin, carrot, squash, and sweet potato showed a lower occurrence of anemia by 17% (OR = 0.83; 95% CI = 0.70-0.99; p = 0.036) compared to those who were not fed these vegetables. Chances of anemia increased by 14% in children who were fed white potatoes, white yams, manioc, cassava, and other root-based foods (OR = 1.14; 95% CI = 1.01-1.29; p = 0.036). CONCLUSION: This study highlighted the impact of a plant-based diet on the high prevalence of childhood anemia in Nigeria. Therefore, reformation of dietary habits, the inclusion of nutritional supplements, and food-fortification programs with reductions in maternal anemia are recommended.

Prevalence and patterns of symptoms of dysautonomia in patients with long-COVID syndrome: A cross-sectional study.

BACKGROUND: The association between autonomic dysfunction and long-COVID syndrome is established. However, the prevalence and patterns of symptoms of dysautonomia in long-COVID syndrome in a large population are lacking. OBJECTIVE: To evaluate the prevalence and patterns of symptoms of dysautonomia in patients with long-COVID syndrome. METHODS: We administered the Composite Autonomic Symptom Score 31 (COMPASS-31) questionnaire to a sample of post-COVID-19 patients who were referred to post-COVID clinic in Assiut University Hospitals, Egypt for symptoms concerning for long-COVID syndrome. Participants were asked to complete the COMPASS-31 questionnaire referring to the period of more than 4 weeks after acute COVID-19. RESULTS: We included 320 patients (35.92 ± 11.92 years, 73% females). The median COMPASS-31 score was 26.29 (0-76.73). The most affected domains of dysautonomia were gastrointestinal, secretomotor, and orthostatic intolerance with 91.6%, 76.4%, and 73.6%, respectively. There was a positive correlation between COMPASS-31 score and long-COVID duration (p < 0.001) and a positive correlation between orthostatic intolerance domain score and post-COVID duration (p < 0.001). There was a positive correlation between orthostatic intolerance domain score and age of participants (p = 0.004). Two hundred forty-seven patients (76.7%) had a high score of COMPASS-31 >16.4. Patients with COMPASS-31 >16.4 had a longer duration of long-COVID syndrome than those with score <16.4 (46.2 vs. 26.8 weeks, p < 0.001). CONCLUSIONS: Symptoms of dysautonomia are common in long-COVID syndrome. The most common COMPASS-31 affected domains of dysautonomia are gastrointestinal, secretomotor, and orthostatic intolerance. There is a positive correlation between orthostatic intolerance domain score and patients' age.

Intravenous iron sucrose vs. blood transfusion in the management of moderate postpartum iron deficiency anemia: A non-randomized quasi-experimental study.

INTRODUCTION: Postpartum anemia is often over-treated with blood transfusion without clear indication despite having a potential alternative of parenteral iron therapy. The present study aimed to compare the efficacy of intravenous (IV) iron sucrose with blood transfusion in increasing the hematological parameters in postpartum women with moderate anemia. METHODS: This prospective non-randomized quasi-experimental study was conducted among 44 hemodynamically stable postpartum women with moderate anemia (Hb 7-8 g/dl) in the Obstetrics department of Dhaka Medical College Hospital (DMCH) from January to June 2021. Among them, 22 patients received 600 mg of IV iron sucrose after 48 h of delivery for three subsequent days and the other 22 patients received two units of blood transfusion after 48 h of delivery in two subsequent days. The primary endpoint was increase in Hemoglobin (Hb) and serum ferritin level after 6 weeks of the intervention. Two-way repeated measures ANOVA (mixed factor ANOVA) was applied to compare between before and after effect in the two intervention groups. RESULTS: Baseline Hb and ferritin were 7.4 g/dl and 73.5 µg/l in IV iron group and 7.3 g/dl and 73.2 µg/l in blood transfusion group. Mean Hb level was increased 4.2 g/dl in IV iron sucrose group and 4.5 g/dl in blood transfusion group at sixth week. Besides, serum ferritin level was increased 40.5 µg/l and 44.8 µg/l after six weeks in IV iron sucrose group and blood transfusion group respectively. Other hematological parameters like reticulocyte count, MCV, MCH, and MCHC also increased significantly after intervention in both groups. However, no significant difference was noticed in the change of hematological parameters in between the groups. CONCLUSIONS: The IV iron sucrose is as effective as blood transfusion in replenishing the hemoglobin and iron storage status in hemodynamically stable women with moderate post-partum anemia. This could be an effective alternative of blood transfusion in treating these patients, especially in resource-poor settings.

Hair, serum and urine chromium levels in children with cognitive defects: A systematic review and meta-analysis of case control studies.

Environmental chromium exposure may cause impaired development of children. We conducted a systematic review and meta-analysis. Electronic databases including PubMed, Embase, Web of Science and CINAHL were searched to identify case-control studies that reported childhood Cr exposure and cognitive development. The Newcastle-Ottawa Scale (NOS) was used to ensure the quality of the included studies. Cr levels were compared in cases and controls, and a random effect meta-analysis was performed using Stata version 16. Twelve of 61 studies identified in the literature search were eligible for this analysis. Hair, serum and urine Cr measurements were reported by seven, two and one studies, respectively. In addition, one study reported both serum and hair Cr exposure and another reported urine and hair Cr exposure. The pooled standard mean differences (SMD) showed that hair Cr levels were non-significantly lower among children with cognitive defects (-0.01 µg/g, 95% CI: -0.04, 00, p = 0.27). In serum and urine, the pooled SMD was higher in children with cognitive deficits compared with healthy control children (0.32 µg/g, 95% CI: -0.78, 1.42, p = 0.56 and 0.64 µg/g, CI: -0.07,1.36, p = 0.08; respectively). In summary, this systematic review found no significant differences in hair, serum and urine Cr levels between children with cognitive deficits and healthy control children when all study data were pooled in the meta-analysis. Larger studies using standardized criteria and longitudinal assessment of cognitive development are needed to determine whether there is a dose response effect of childhood Cr exposure on cognitive development of children.

Structural modeling to understand the relationship among food safety knowledge, attitude, and self-reported HACCP practices in restaurant employees in Bangladesh.

In this materialistic era, a substantial number of people are likely to have their meals outside of their homes and largely depend on the restaurants' made food due to the prolonged working hours and tremendous pressure in workplace. Consequently, consumers expose themselves to risk and become vulnerable to illness caused by food. Unsafe food preparation and handling by restaurants' workers have made food safety concern for public health. The study was aimed to examine the relationships among food safety knowledge, attitude, and Hazard Analysis Critical Control Point (HACCP) practices in restaurant employees in Bangladesh. A total of 360 employees from 120 restaurants participated in a face-to-face interview through a structured questionnaire comprising four sections: demographic characteristics, food safety knowledge, attitudes and practices. The mean scores for each survey item were calculated and used in Structural Equation Modeling (SEM), designed to assess interrelationships among the three sections related to food safety. Participants obtained a correct average score of 53.32% in food safety knowledge, with the highest and lowest correct scores in Good Hygiene Practices (GHPs) and HACCP practices, respectively. The highest score in the attitude section belonged to "self-improvement", followed by "food safety concern". A negative correlation was observed between knowledge with practices, knowledge with attitudes, and a positive correlation was observed between practices with attitudes. A significant positive correlation was observed between HACCP practices with shelf improvement (r = 0.54, p < 0.05) and the knowledge toward food poisoning with GHP practices (r = 0.55, p < 0.05). Self-improvement and food safety concerns are negatively correlated with food poisoning, GHP, and HACCP practice. This study demonstrated that restaurant employees in Bangladesh often lack knowledge regarding food safety and HACCP. So, in order to improve knowledge and attitude on safe HACCP practice among the restaurants employees', proper education and interactive training sessions can be conducted.

Inequality, chronic undernutrition, maternity, and diabetes mellitus as the determinant of anemia among ever-married women in Bangladesh.

BACKGROUND: Anemia is a public health concern at a global level. This study aims to delineate the association between household economic inequalities, and anemia among reproductive age group women in Bangladesh, along with other confounders. METHODS: A cross-sectional population sample from Bangladesh Demographic and health survey data comprising of 5920 reproductive age group women aged between 15 and 49 years was used in this study. The analyses were performed through the application of proportional odds into four models (viz., Model:1 socio-economic, Model:2 socio-demographic factors, Model 3: diabetics and maternity statis, Model 4: nutritional status.) RESULTS: The respondents from low and medium socioeconomic status (SES) households vs. richest households were 1.90 (95% CI, 1.65,2.17; p < .01) and 1.41 (95% CI, 1.12,1.78; p < .01) times more likely to suffer from anemia (Model 1). From the model 2 it appears that he likelihood of being anemic reduces for the low and medium SES groups vs. high SES group when sociodemographic variables are added (OR, 1.69, 95% CI, 1.43,1.99 and OR, 1.35, 95% CI, .07,1.70; p < 0.01, respectively). Model 3 evident that after adding the variables of diabetes and maternity status, the association between having anemia belonging to low and medium SES vs. high SES becomes weaker (OR1.36, 95% CI,0.85,2.15 and OR, 1.15, 95% CI, 0.6,2.19; p > .05, respectively). Moreover, the strength of the association between anemia and the subjects with pregnant vs. the subjects without these (OR: 1.7 (1.12, 4.02; p < 0.05) 1.47(0.11,3.399; p < 0.05) conditions was reduced after factoring body mass index (BMI, model 4). Besides, thin women (MBI < 18.50 Kg/m2) shows significantly become more likely to be anemic in comparison to women of normal BMI (OR:1.34, .92,1.96; p < 0.05) (model 4). CONCLUSIONS: BMI, pregnancy, and diabetes mellitus were observed to be significantly associated with anemia, and the strength of the association was low with anemia and socioeconomic inequality.

Study of nitrate levels in fruits and vegetables to assess the potential health risks in Bangladesh.

Nitrate is a chemical compound naturally present in fruits and vegetables. This study aims at assessing the nitrate levels and health risks arising from high consumption of fruits and vegetables in Bangladesh. Sixteen species of fruits and vegetables were examined for nitrates using High-Performance Liquid Chromatography with Photo Diode Array (PDA) detector. Ward's hierarchical cluster analysis was carried out to identify the cluster of tested fruits and vegetables for the nitrate contents. A point estimate of the daily intake was applied to find the health risks that arise due to elevated levels of nitrate in fruits and vegetables. The results show that root and tuber vegetables accumulate significantly higher levels of nitrate in comparison to fruits and fruit vegetables (P < 0.05). In cluster analysis, the nitrate accumulation of fruits and vegetables show four clear clusters contributing to 29.54%, 7.17%, 4.42%, and 58.57% of the total nitrate content in the entire sample. The risk assessment of the Estimated Daily Intake (EDI) and Health Risk Index (HRI) of almost all the tested samples was in the acceptable range, except for radish, thereby indicating the acceptance of risk due to nitrate intake in Bangladesh. As nitrate may have had risk factor for health, during cultivation and storing the product should be properly monitored.

Development of potential map for groundwater abstraction in the northwest region of Bangladesh using RS-GIS-based weighted overlay analysis and water-table-fluctuation technique.

The increasing trend of population growth along with the rapid groundwater-based agricultural expansion and decreasing trend of mean annual rainfall in the Northwest region of Bangladesh has been exacerbating the declination of groundwater for further expansion. Therefore, the present study attempts to demarcate the potential groundwater abstraction zones from the assessment of potential recharge and available recharge. Potential recharge was obtained with commonly used geospatial-based weighted linear combination (WLC) technique. Here, WLC analysis was based on eight factors related to physiographic (e.g. drainage density, lineament density, slope), geomorphologic (e.g. geomorphology, lithology, soil), land use and land cover (LULC) and hydrology (i.e. rainfall). Available net recharge was assessed for the period 1993-2017 by employing the water table fluctuation method. Finally, the resultant map on potential abstraction was characterized into five different classes, viz. 'very low', 'low', 'moderate', 'high' and 'very high'. The derived map reveals that 'very high' potential zone is distributed along the Teesta river floodplain, especially the northeastern part. In contrast, the Barind Tract (i.e. the southwestern and the southcentral parts) area shows 'very low' groundwater prospect. Such fused interpretations are expected to contribute to the planning of integrated management of water resources.

Highly Stretchable and Flexible Melt Spun Thermoplastic Conductive Yarns for Smart Textiles.

This study demonstrates a scalable fabrication process for producing biodegradable, highly stretchable and wearable melt spun thermoplastic polypropylene (PP), poly(lactic) acid (PLA), and composite (PP:PLA = 50:50) conductive yarns through a dip coating process. Polydopamine (PDA) treated and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) coated conductive PP, PLA, and PP/PLA yarns generated electric conductivity of 0.75 S/cm, 0.36 S/cm and 0.67 S/cm respectively. Fourier Transform Infrared Spectroscopy (FTIR) confirmed the interactions among the functional groups of PP, PLA, PP/PLA, PDA, and PEDOT:PSS. The surface morphology of thermoplastic yarns was characterized by optical microscope and Scanning Electron Microscope (SEM). The mechanical properties of yarns were also assessed, which include tensile strength (TS), Young's modulus and elongation at break (%). These highly stretchable and flexible conductive PP, PLA, and PP/PLA yarns showed elasticity of 667%, 121% and 315% respectively. The thermal behavior of yarns was evaluated by differential scanning calorimetry (DSC) and thermo-gravimetric analysis (TGA). Wash stability of conductive yarns was also measured. Furthermore, ageing effect was determined to predict the shelf life of the conductive yarns. We believe that these highly stretchable and flexible PEDOT:PSS coated conductive PP, PLA, and PP/PLA composite yarns fabricated by this process can be integrated into textiles for strain sensing to monitor the tiny movement of human motion.

Association of Socioeconomic Status With Childhood Anemia Among Infant, Toddler, and Preschool Children in Bangladesh.

OBJECTIVES: This study aims to delineate the association between economic inequality, along with other confounders, and anemia among infants, toddlers, and preschool children. METHODS: From the 2011 Bangladesh Demographic Health Survey, a cross-sectional population sample comprising 2068 children between ages 6 and 59 months were selected for this study. Analyses were performed with a proportional odds model and finally stratified with the child age groups. RESULTS: Infants belonging to a low and medium socioeconomic status (SES) have approximately a 3-fold higher chance of being affected by mild, moderate, or severe anemia compared with infants of high SES (odds ratio [OR] 2.94; 95% CI 1.09-7.91; P=.03 and OR 2.76; 95% CI .87-8.82; P=.08, respectively). Preschool children from low and medium SES households are 2.73× (95% CI 1.20-6.18; P=.02) and 2.47× (95% CI .99-6.14; P=.04) more likely to be anemic compared with their counterparts from higher SES households. The place of residence and childhood stunting are associated with childhood anemia (urban vs rural: OR 1.27; 95% CI .21-.35; P = .04; and stunted vs normal: OR 1.34; 95% CI 1.11-1.63; P = .003). Besides, vitamin A supplementations appear to serve as protective agents against the occurrence of the childhood anemia (OR 1.18; 95% CI .99-1.41; P = .06). CONCLUSION: Urgent preventive measures are needed to control the impending childhood anemia among infants and preschool children, especially in the low and medium SES households (ClinicalTrials.gov Identifier: NCT03126253).

Detection of changes and trends in climatic variables in Bangladesh during 1988-2017.

Due to the importance of climatic variability, an assessment detecting the changes and trends has been carried out over different time series of major climatic variables from the records of meteorological stations over Bangladesh from 1988-2017. Linear regression, the Mann-Kendall test, and Sen's slope method were used to analyze the significant trends and magnitude of the variables' changes, while the Pearson and Spearman rho correlation test have been applied to correlate between the variables. The results show that the average monthly maximum temperature (T max ) and minimum temperature (T min ) have increased significantly by 0.35 °C/decade and 0.16 °C/decade, respectively. However, the increase in T max is comparatively higher than T min and caused significant increases in the monthly temperature range (MTR) at a higher rate in winter than in the monsoon season. The trend patterns of T max , T min , and MTR reveal that most of the regions of the country (especially the south-eastern and north-eastern) have been colder during winter and hotter during the monsoon. In contrast, the wind speed (WS) has decreased significantly all over the country and decreased by a higher rate in the north-western (NW) region (monsoon, 0.60 and annual, 0.51 kt/decade) than other regions, while the monsoonal and annual precipitation have decreased by 87.35 mm/decade and 107 mm/decade, respectively. The monsoonal T max and T min (0.47 °C/decade and 0.38 °C/decade, respectively) have increased significantly in the NW; consequently, this region has been warmed by 0.27 °C/decade. The increase in temperature and decrease in WS may cause a decrease in rainfall in the NW region. Humidity changes are not significant except in the monsoon season across the country. Precipitation, WS, and humidity are negatively correlated with the temperature variables. The declination of WS may influence the rising trend in temperature and the falling trend in precipitation and humidity, suggesting the need for further advanced study on the negative effects of climate change in Bangladesh.

The effect of tetracycline on the structure of the bacterial community in a wastewater treatment system and its effects on nitrogen removal.

This study examined the impact of tetracycline at two environmentally relevant concentrations (1 µg/L and 10 µg/L) and one synthetically high concentration (500 µg/L) on the structure and function of the microbial community from the secondary treatment process of a municipal wastewater treatment plant (WWTP). Specifically, this study examined whether the introduction of tetracycline into bench scale reactors at two different replacement volume rates would cause a shift in the composition profile of the bacterial community. Furthermore concentrations of ammonia, nitrate/nitrite and total Kjeldahl nitrogen were monitored to examine the effect of the antibiotic on ammonia and nitrogen removal. At the low volume replacement rate, tetracycline was observed to have a positive impact on nitrogen removal. Total Kjeldahl nitrogen concentrations were also observed to decrease suggesting a role for tetracycline as a carbon source. However, at the higher volume replacement rate, the removal of ammonia and nitrogen were not significantly different from reactors that did not contain tetracycline. Over time, the bacterial composition profiles changed under all the conditions studied, however, the bacterial composition profiles appeared to be more influenced by the replacement volume rate than the presence of tetracycline even at concentrations many times higher than environmentally relevant amounts.

Inequality and Mother's Age as Determinants of Breastfeeding Continuation in Bangladesh.

The World Health Organization recommends continuing breastfeeding up to 2 years of age or beyond for sound growth and development of children. In Bangladesh, continuation rates for breastfeeding have recently decreased and effective measures are required to counter this downward trend. Although recent years have seen economic development and reductions in poverty, Bangladesh still has the highest rate of child marriage worldwide. Thus, we aimed to clarify the factors influencing breastfeeding continuation, especially from the perspective of inequality and mother's age in Bangladesh, using data from the Bangladesh Demographic and Health Survey 2011. Event history analyses were performed during a 24-month follow-up period on 7,041 mothers with duration of breastfeeding as the outcome variable, with wealth index (an indicator for inequality) and mother's age used as the main explanatory variables. The results showed that poorer mothers were on the whole more likely to continue breastfeeding through 24 months after childbirth, and younger mothers were less likely to continue breastfeeding particularly past the first year. However, both younger and older mothers continued breastfeeding to the same extent within the first year after childbirth. Mother's age had time-varying effects on breastfeeding continuation, meaning that the effects on breastfeeding continuation were affected by the child's age. These findings imply that policymakers should be aware that efforts to reduce child marriage may increase the rate of breastfeeding continuation beyond the first year after childbirth. In contrast, efforts at poverty alleviation, aimed at preventing child marriage, may decrease the rate irrespective of the child's age.

Effects of tetracycline and ibuprofen on the relative abundance of microbial eukaryotic and bacterial populations in wastewater treatment.

The activated sludge process in a wastewater treatment plant (WWTP) relies on the activity of microbes to reduce the organic and inorganic matter and produce effluent that is safe to discharge into receiving waters. This research examined the effects of the non-steroidal anti-inflammatory drug ibuprofen and the antibiotic tetracycline on the relative abundance and composition of eukaryotes and bacteria in the microbial population present in activated sludge from a WWTP. The current investigation was designed to observe the impact of these contaminants, at low (environmentally relevant concentrations) as well as high concentrations of the drugs. Using 16S and 18S rRNA gene primer sets and quantitative polymerase chain reaction, the abundance of each population was monitored as well as the relative ratio of the two populations under the various conditions. It was found that current environmentally relevant concentrations of ibuprofen (100 ng/mL) stimulated eukaryotic growth but higher concentrations (2,000 ng/mL, 100,000 ng/mL) reduced their numbers significantly especially in the presence of tetracycline. Finally using denaturing gradient gel electrophoresis, some of the more abundant eukaryotes were identified and it was noted that high ibuprofen and tetracycline concentrations favoured the abundance of some genera.

A modular vascular access training program for higher surgical trainees.

INTRODUCTION: Access surgery is delivered by vascular/transplant surgeons with a division that is defined by historic practice. This has resulted in an inconsistent training pattern. We aimed to design a focused, modular training program (The Brighton Vascular Access Fellowship) providing trainees with a reproducible level of exposure and competence. METHODS: The programme was 16 days over 8 weeks on a one-to-one basis with candidates expected to be performing procedures as first surgeon with ongoing, formative assessment. The outpatient setting took the format of a one-stop clinic to involve planning and the follow-up. Assessment was through caseload exposure and conventional statistical analyses to obtain median values (as proxy measures of training exposure consistency). Assessment of confidence and capability was through an electronically distributed qualitative survey tool. RESULTS: A total of 14 candidates completed the programme by June 2017. Operative exposure was obtained for 11 (79%) with a total of 471 cases of which 286 were conducted as first surgeon. There was a median of 32 cases by each candidate with 25 of the cases performed as first surgeon. Qualitative assessment revealed that 13 of 14 (93%) were either practicing independently or no longer required the trainer to scrub in for the operation. A total of 13 of 14 (93%) strongly agreed that they felt comfortable with offering a basic access service. CONCLUSIONS: Focused modular training might be one of the answers to the current era of restricted and sometimes inconsistent training in some aspects of surgery. This model is reproducible and may be applicable in other aspects of training.