A ballistic pollen dispersal strategy based on stylar oscillation of Hypochaeris radicata (Asteraceae).

Asteraceae, one of the largest flowering plant families, are adapted to a vast range of ecological niches. Their adaptability is partially based on their strong ability to reproduce. The initial, yet challenging, step for the reproduction of animal-pollinated plants is to transport pollen to flower-visiting pollinators. We adopted Hypochaeris radicata as a model species to investigate the functional morphology of the typical floral feature of Asteraceae, a pollen-bearing style. Using quantitative experiments and numerical simulations, here we show that the pollen-bearing style can serve as a ballistic lever for catapulting pollen grains to pollinators. This can potentially be a pollen dispersal strategy to propel pollen to safe sites on pollinators' bodies, which are beyond the physical reach of the styles. Our results suggest that the specific morphology of the floret and the pollen adhesion avoid pollen waste by catapulting pollen within a specific range equal to the size of a flowerhead. The insights into the functional floral oscillation may shed light on the superficially unremarkable, but ubiquitous functional floral design of Asteraceae.

Attachment-based mechanisms underlying capture and release of pollen grains.

Successful insect pollination can be achieved by a sequence of numerous attachment and detachment events at various biological surfaces. However, the quantitative measurements of pollen adhesion on biological surfaces have been poorly studied so far. We performed atomic force microscopy adhesion measurements of pollen on two most important floral parts for Asteraceae in a course of pollination: the stigma and style of Hypochaeris radicata plant. The results indicated distinct adhesive properties of them-the pollen adhesion on stigmatic surfaces drastically increased over prolonged contact time, while the pollen adhesion increase on stylar surfaces was rather restrained. Based on the observation with cryo-scanning electron microscopy, we explained the experimental results by the presence of morphological features in form of flexible stigmatic papillae that may play a crucial role in enhancing both capillary attraction and van der Waals forces. The distinct adhesive properties seemingly originate from the unique adhesive tasks that each of the floral parts requires to achieve successful pollination. The insights into the adhesive interaction between pollen and the floral parts, obtained in the present study, may lead to better understanding of pollination mechanisms, which are strongly related to our food production. Additionally, the novel pollen adhesive mechanisms learned from the stigma of the studied Asteraceae plant can inspire biomimetic designs of spontaneous gripping systems.

Fresh "Pollen Adhesive" Weakens Humidity-Dependent Pollen Adhesion.

This study presents a quantitative investigation of pollen adhesion mediated by pollenkitt using pollen grains in their native state. Here, we attempt to clarify whether the exposure time, pollenkitt losses, and the surrounding humidity (relative humidity, RH) levels influence pollen adhesion. Pollen grains of Hypochaeris radicata (Asteraceae) were tested using atomic force microscopy. Regardless of the pollen condition (fresh, aged, and without pollenkitt), higher RH significantly increased pollen adhesion on hydrophilic surfaces, whereas it had little effect on pollen adhesion on hydrophobic surfaces. On hydrophilic surfaces, adhesion of fresh pollen was less dependent on RH than that of aged pollen or without pollenkitt, resulting in reduced adhesion under high RH. On hydrophobic surfaces, adhesion of fresh pollen was significantly lower than that of aged pollen. We utilized capillary models to explain the counterintuitive results obtained and came to the conclusion that the abundant fresh pollenkitt, which is widely accepted as pollen adhesive, can reduce pollen adhesion in some conditions. This study sheds light on the little-known adhesive properties of pollen and on the pollination mechanics.

Effects of Playing Position on Hydration Status in Collegiate Marching Band Musicians.

OBJECTIVE: Marching band musicians often endure prolonged daily practices in warm-hot outdoor environments. Evaluation of hydration status by instrument position can shed light on health-related issues for these performers. The objective of this study was: a) to determine the effects of playing position on hydration status based on urinary biomarkers (urinary specific gravity and urinary osmolality) before and after marching band practice, and b) to evaluate the relation of hydration status with body mass change and fluid consumption for all playing positions. METHODS: Fifty-eight collegiate marching band players participated in this study, involving five playing positions: band pit (n=10), baritone and euphonium (n=12), snare drum (n=13), trumpet (n=12), and tuba (n=11). All participants performed their own routine marching band practice, which lasted a total of 6 hrs on 1 day. Each individual consumed ad libitum commercially available carbohydrate-electrolyte solution during the practice. To determine hydration status, urine samples were collected before and after practice for analysis of urinary specific gravity and urinary osmolality. Body weight and total fluid intake were also assessed pre- and post-exercise. RESULTS: There were no significant effects for playing position and time or interaction concerning the levels of urinary osmolality and specific gravity after practice. CONCLUSIONS: Our main findings suggest that hydration status may be similar among the different playing positions following prolonged practice in the outdoor environment.

Capillary rise on legs of a small animal and on artificially textured surfaces mimicking them.

The wharf roach Ligia exotica is a small animal that lives by the sea and absorbs water from the sea through its legs by virtue of a remarkable array of small blades of micron scale. We find that the imbibition dynamics on the legs is rather complex on a microscopic scale, but on a macroscopic scale the imbibition length seems to simply scale linearly with elapsed time. This unusual dynamics of imbibition, which usually slows down with time, is advantageous for long-distance water transport and results from repetition of unit dynamics. Inspired by the remarkable features, we study artificially textured surfaces mimicking the structure on the legs of the animal. Unlike the case of the wharf roach, the linear dynamics were not reproduced on the artificial surfaces, which may result from more subtle features on the real legs that are not faithfully reflected on the artificial surfaces. Instead, the nonlinear dynamics revealed that hybrid structures on the artificial surfaces speed up the water transport compared with non-hybrid ones. In addition, the dynamics on the artificial surfaces turn out to be well described by a composite theory developed here, with the theory giving useful guiding principles for designing hybrid textured surfaces for rapid imbibition and elucidating physical advantages of the microscopic design on the legs.