RESUMO
We designed a new complex drug with antiallergic effect containing, in addition to the main component loratadine, a phytocomplex for an extra therapeutic effect. A collection of plants with sedative activity is chosen and the optimal agent for extraction of bioactive compounds (40% ethanol) and optimal degree of plant fragmentation are determined. Chemical composition of the sedative tea is evaluated by reverse phase HPLC. The marker components of the species are detected: xanthohumol and isoxanthohumol - Humulus lupulus cone components, Mentha piperita rosmarinic acid, and scutellareine, Menyanthes trifolia element - quercetin-3-rutinoside, and caffeic acid. Standardization of the species by the absolute graduation method in conversion to quercetin-3-rutinoside is suggested.
Assuntos
Antagonistas dos Receptores Histamínicos H1/isolamento & purificação , Humulus/química , Mentha piperita/química , Compostos Fitoquímicos/isolamento & purificação , Supositórios/síntese química , Apigenina/química , Apigenina/isolamento & purificação , Ácidos Cafeicos/química , Ácidos Cafeicos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Cinamatos/química , Cinamatos/isolamento & purificação , Depsídeos/química , Depsídeos/isolamento & purificação , Etanol/química , Flavonoides/química , Flavonoides/isolamento & purificação , Antagonistas dos Receptores Histamínicos H1/química , Antagonistas dos Receptores Histamínicos H1/normas , Humanos , Compostos Fitoquímicos/química , Compostos Fitoquímicos/normas , Extratos Vegetais/química , Propiofenonas/química , Propiofenonas/isolamento & purificação , Rutina/química , Rutina/isolamento & purificação , Solventes/química , Xantonas/química , Xantonas/isolamento & purificação , Ácido RosmarínicoRESUMO
Growth peculiarities of the strain of homoenzymic lactic acid bacteria Enterococcus faecium under periodic cultivation have been studied. The effect of carbohydrates source on the level of biomass accumulation and efficiency of its use by the strain is shown. Glucose concentration in the medium being increased from 1.0 to 3.5%, the economic effect decreases from 16.0 to 10.6%. It is established that only at the beginning of cultivation the specific growth rate of the strain is determined by the concentration of lactic acid. This dependence can be described by Ierusalimsky's equation. The obtained values of micronm and Kp are 1.5 h(-1) and 6.5 g/l, respectively. Biomass concentration being above 0.3 g ACB/l, the strain growth depends on two factors: inhibition of lactic acid and limitation of the sources of nitrogen nutrition. Interaction of these two factors results in considerable decrease of specific rate of bacterial growth.