RESUMO
The NSL complex is a transcriptional activator. Germline-specific knockdown of NSL complex subunits NSL1, NSL2, and NSL3 results in reduced piRNA production from a subset of bidirectional piRNA clusters, accompanied by widespread transposon derepression. The piRNAs most transcriptionally affected by NSL2 and NSL1 RNAi map to telomeric piRNA clusters. At the chromatin level, these piRNA clusters also show decreased levels of H3K9me3, HP1a, and Rhino after NSL2 depletion. Using NSL2 ChIP-seq in ovaries, we found that this protein specifically binds promoters of telomeric transposons HeT-A, TAHRE, and TART Germline-specific depletion of NSL2 also led to a reduction in nuclear Piwi in nurse cells. Our findings thereby support a role for the NSL complex in promoting the transcription of piRNA precursors from telomeric piRNA clusters and in regulating Piwi levels in the Drosophila female germline.
Assuntos
Proteínas de Drosophila , RNA de Interação com Piwi , Animais , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Drosophila/genética , Telômero/genética , Telômero/metabolismoRESUMO
Nucleosomal organization at gene promoters is critical for transcription, with a nucleosome-depleted region (NDR) at transcription start sites (TSSs) being required for transcription initiation. How NDRs and the precise positioning of the +1 nucleosomes are maintained on active genes remains unclear. Here, we report that the Drosophila nonspecific lethal (NSL) complex is necessary to maintain this stereotypical nucleosomal organization at promoters. Upon NSL1 depletion, nucleosomes invade the NDRs at TSSs of NSL-bound genes. NSL complex member NSL3 binds to TATA-less promoters in a sequence-dependent manner. The NSL complex interacts with the NURF chromatin remodeling complex and is necessary and sufficient to recruit NURF to target promoters. Not only is the NSL complex essential for transcription, but it is required for accurate TSS selection for genes with multiple TSSs. Furthermore, loss of the NSL complex leads to an increase in transcriptional noise. Thus, the NSL complex establishes a canonical nucleosomal organization that enables transcription and determines TSS fidelity.
Assuntos
Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Nucleossomos/genética , Transcrição Gênica/genética , Animais , Proteínas de Drosophila/genética , Drosophila melanogaster/metabolismo , Proteínas Nucleares , Regiões Promotoras Genéticas/genética , Fatores de Transcrição/genética , Iniciação da Transcrição Genética , Proteínas de Transporte VesicularRESUMO
Heat shock response (HSR) is an important element of cellular homeostasis. In yeast, HSR comprises of the heat shock proteins (Hsps) and the osmolytes trehalose and glycerol. The respective roles of trehalose and Hsp104 in regulating protein aggregation remain ambiguous. We report that trehalose and Hsp104 are important during the early stages of protein aggregation, i.e. when the process is still reversible. This corroborates the earlier reported role of trehalose being an inhibitor of protein folding. Under in vitro conditions, trehalose is able to restore the GdHCl-induced loss of ATPase activity of recombinant Hsp104 to almost its original level. As the saturation phase of aggregation approaches, neither of the two components is able to exert any effect. Inactivation of Hsp104 at the stage when oligomers have already been formed increases the rate of formation of aggregates by inhibiting disaggregation of oligomers. In the absence of an active disaggregase, the oligomers are converted to mature irreversible aggregates, accelerating their formation. Our results suggest that the disaccharide may have a marginally stronger influence than Hsp104 in inhibiting protein aggregation in yeast cells.