RESUMO
OBJECTIVE: To evaluate the effect of surgical intervention on serum insulin-like growth factor 1 levels in patients with obstructive sleep apnoea. METHODS: A prospective study was conducted in a tertiary care hospital of adult patients with obstructive sleep apnoea for whom continuous positive airway pressure therapy failed or was refused. All patients underwent polysomnography and serum insulin-like growth factor 1 evaluation pre-operatively and at three months post-operatively. The site of surgery was determined using Müller's manoeuvre and ApneaGraph AG 200. RESULTS: Fifteen patients were included with a mean age of 38 years: 11 males and 4 females. The mean pre-operative Apnoea-Hypopnoea Index using polysomnography was 53.7 events per hour, and the mean post-operative Apnoea-Hypopnoea Index at three months was 15.3 events per hour (p = 0.0001). The mean pre-operative serum insulin-like growth factor 1 was 160.2 µg/l, while the mean post-operative value was 236.98 µg/l (p = 0.005). CONCLUSION: In adult patients with obstructive sleep apnoea for whom continuous positive airway pressure therapy fails, site-specific surgical intervention to treat the obstruction leads to an increase in serum insulin-like growth factor 1 levels.
Assuntos
Fator de Crescimento Insulin-Like I , Apneia Obstrutiva do Sono , Adulto , Feminino , Masculino , Humanos , Estudos Prospectivos , Apneia Obstrutiva do Sono/cirurgia , Pressão Positiva Contínua nas Vias Aéreas , PolissonografiaRESUMO
OBJECTIVE: To elucidate the aetiopathogenesis of facial neuritis in coronavirus disease 2019 associated mucormycosis. METHODS: A retrospective review was conducted of coronavirus disease 2019 associated mucormycosis patients who presented with peripheral facial nerve palsy from January 2021 to July 2021. The clinico-radiological details of four patients were assessed to examine the potential mechanism of facial nerve involvement. RESULTS: Serial radiological evaluation with contrast-enhanced computed tomography and contrast-enhanced magnetic resonance imaging revealed infratemporal fossa involvement in all cases, with the inflammation extending along fascial planes to reach the stylomastoid foramen. Ascending neuritis with an enhancement of the facial nerve was demonstrated in all cases. CONCLUSION: The likely explanation for facial palsy in patients with coronavirus disease 2019 associated mucormycosis, backed by radiology, is the disease abutting the facial nerve at the stylomastoid foramen and causing ascending neuritis of the facial nerve.
Assuntos
COVID-19 , Doenças do Nervo Facial , Paralisia Facial , Mucormicose , Neurite (Inflamação) , Radiologia , COVID-19/complicações , Nervo Facial/diagnóstico por imagem , Nervo Facial/patologia , Paralisia Facial/etiologia , Humanos , Imageamento por Ressonância Magnética/efeitos adversos , Mucormicose/complicações , Mucormicose/diagnóstico por imagem , Neurite (Inflamação)/complicações , Neurite (Inflamação)/patologiaRESUMO
In earlier studies, we have shown that cigarette smoke condensate (CSC), a surrogate for cigarette smoke, is capable of transforming the spontaneously immortalized human breast epithelial cell line, MCF10A. These transformed cells displayed upregulation of the anti-apoptotic gene, bcl-xl. Upregulation of this gene may impede the apoptotic pathway and allow the accumulation of DNA damage that can lead to cell transformation and carcinogenesis. In the present study, we have determined the mechanism of CSC-mediated transcriptional upregulation of bcl-xl gene expression in MCF10A cells. We cloned the human bcl-xl promoter (pBcl-xLP) and identified putative transcription factor binding sites. Sequential deletion constructs that removed the putative cis-elements were constructed and transfected into MCF10A cells to determine the CSC-responsive cis-element(s) on the pBcl-xLP. Gel-shift, super-shift and chromatin immunoprecipitation analysis confirmed that CCAAT/enhancer-binding protein (C/EBPbeta) specifically bound to a C/EBP-binding site on the pBcl-xLP in vitro and in vivo. Additionally, overexpression of C/EBPbeta-LAP2 stimulated pBcl-xLP activity and Bcl-xL protein levels, which mimicked the conditions of CSC treatment. Our results indicate that C/EBPbeta regulates bcl-xl gene expression in MCF10A cells in response to CSC treatment; therefore, making it a potential target for chemotherapeutic intervention of cigarette smoke-induced breast carcinogenesis.
Assuntos
Mama/metabolismo , Proteína beta Intensificadora de Ligação a CCAAT/metabolismo , Células Epiteliais/metabolismo , Regulação Neoplásica da Expressão Gênica , Regulação da Expressão Gênica , Fumar , Proteína bcl-X/biossíntese , Sequência de Bases , Sítios de Ligação , Linhagem Celular Tumoral , Dano ao DNA , Humanos , Dados de Sequência Molecular , Regiões Promotoras Genéticas , FumaçaRESUMO
Our previous studies have shown that treatment with cigarette smoke condensate (CSC) transforms normal breast epithelial cell line, MCF-10A. In the present study, the mechanism of CSC-induced transformation of breast epithelial cells was examined. We first determined whether benzo[a]pyrene (B[a]P)- and CSC-induced levels of APC are capable of inhibiting long-patch base excision repair (LP-BER) since our earlier studies had shown that an interaction of APC with DNA polymerase beta (pol-beta) blocks strand-displacement synthesis. With the use of a novel in vivo LP-BER assay, it was demonstrated that increased and decreased APC levels in different breast cancer cell lines were associated with a decrease or increase in LP-BER activity, respectively. The effect of APC on LP-BER in malignant and pre-malignant breast epithelial cell lines was produced by either overexpression or knockdown of APC. Furthermore, it was shown that the decreased LP-BER in B[a]P- or CSC-treated pre-malignant breast epithelial cells is associated with an increased level of APC and decreased cell growth. Our results suggest that the decreased growth allows cells to repair the damaged DNA before mitosis, and failure to repair damaged DNA has the potential to transform pre-malignant breast epithelial cells.
Assuntos
Proteína da Polipose Adenomatosa do Colo/fisiologia , Neoplasias da Mama/genética , Mama/metabolismo , Transformação Celular Neoplásica , Reparo do DNA , Nicotiana , Fumaça/efeitos adversos , Benzo(a)pireno/farmacologia , Linhagem Celular , Linhagem Celular Tumoral , DNA Polimerase beta , Células Epiteliais , Humanos , RNA Interferente Pequeno/farmacologiaRESUMO
Treatment of colon cancer cells with MNNG causes DNA damage with reduced telomeric signals in a p53-dependent manner, but increased cell cycle arrest in S-G(2)/M by both p53-dependent and independent mechanisms. Results also indicate that cellular levels of TRF2 may play a critical role in MNNG-induced cell cycle arrest and apoptosis of colon cancer cells.
Assuntos
Ciclo Celular/efeitos dos fármacos , Neoplasias do Colo/metabolismo , Dano ao DNA , Proteínas de Ligação a DNA/metabolismo , Metilnitronitrosoguanidina/toxicidade , Células Tumorais Cultivadas/efeitos dos fármacos , Proteína Supressora de Tumor p53/metabolismo , Apoptose , Western Blotting , DNA/metabolismo , Citometria de Fluxo , Humanos , Hibridização in Situ Fluorescente , Reação em Cadeia da Polimerase , Proteína 2 de Ligação a Repetições Teloméricas , Proteína Supressora de Tumor p53/genéticaRESUMO
In response to DNA damage, p53 protein transiently stabilizes and accumulates in the nucleus, where it performs its role as a transcription factor. Phosphorylation of p53 increases its sequence-specific DNA-binding activity. In the present study, we have examined the effect of methylmethane sulfonate (MMS) to HCT-116 human colon cancer cells on the phosphorylation of p53. Results show that p53 protein becomes phosphorylated at serine 15 (Ser15) and Ser392 residues after treatment with MMS in a time-dependent manner. Increased levels of phospho-p53(Ser15) and phospho-p53(Ser392) were maintained up to 50 h of the MMS treatment. We also examined the involvement of probable kinase(s), which could be responsible for MMS-induced phosphorylation of p53 at Ser15 and Ser392. In vitro phosphorylation assay, carried out with the immunoprecipates of MMS-treated cells, showed an increased phosphorylation of p53 by c-Jun kinase 1 (JNK1) at early time points (2.5 h). However, with cyclin-dependent kinase (Cdk2) and TFIIH complex associated kinase CAK, the phosphorylation of p53 was increased at later time points (25 h). The phosphorylation of p53 by Cdc2 and MAPK (p38) kinases remained unaffected in the MMS-treated versus untreated cells. The MMS-induced phosphorylation of p53 correlates with our previous findings of p53's ability for increased sequence-specific DNA-binding and transcriptional activity in the cells treated with DNA alkylating agents.
Assuntos
Quinases relacionadas a CDC2 e CDC28 , Neoplasias do Colo/metabolismo , DNA de Neoplasias/metabolismo , Fatores de Transcrição TFII , Proteína Supressora de Tumor p53/metabolismo , Alquilação , Antineoplásicos Alquilantes/farmacologia , Western Blotting , Quinase 2 Dependente de Ciclina , Quinases Ciclina-Dependentes/metabolismo , Glutationa Transferase/metabolismo , Humanos , Técnicas In Vitro , Metanossulfonato de Metila/farmacologia , Proteína Quinase 8 Ativada por Mitógeno , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Fosforilação , Proteínas Serina-Treonina Quinases/metabolismo , Fator de Transcrição TFIIH , Fatores de Transcrição/metabolismo , Células Tumorais Cultivadas/efeitos dos fármacos , Proteínas Quinases p38 Ativadas por MitógenoRESUMO
The APC (adenomatous polyposis coli) gene product is involved in cell cycle arrest and in apoptosis. The loss of APC function is associated with the development of colorectal carcinogenesis. In previous studies, we have shown that the APC gene is inducible and that the DNA damage-induced level of APC mRNA requires p53. In the present study, we examined the role of p53 in the transcriptional regulation of APC promoter and characterized two p53-binding sites on the cloned APC promoter (pAPCP). Results of electrophoretic mobility shift assay showed specific interactions of p53 protein with p53-binding site oligonucleotides. The DNA-protein complex formed in electrophoretic mobility shift assay was competed with unlabeled excess of p53-binding site oligonucleotide, unaffected with p53-binding site mutant or Sp1-binding site oligonucleotides, and supershifted with anti-p53 antibodies. In a transient transfection assay, the pAPCP promoter activity was lower in HCT-116(p53(+/+)) cells versus HCT-116(p53(-/-)) cells. p53-dependent down-regulation was further confirmed after co-transfection of pAPCP plasmid with pCMV-p53 into HCT-116(p53(-/-)) and SAOS-2 (p53-negative) cells. However, the treatment of cells with DNA alkylating agents methylmethane sulfonate and N-methyl-N'-nitro-N-nitrosoguanidine, which cause phosphorylation of p53 at Ser(15) and Ser(392), induced pAPCP promoter activity in HCT-116(p53(+/+)) cells. Other than p53-binding sites, using deletion mutation constructs, we have shown that N-methyl-N'-nitro-N-nitrosoguanidine-induced transcriptional activation of the pAPCP promoter in HCT-116(p53(+/+)) cells depended upon the Sp1-binding site and the E-box B site. From these results, we conclude that unphosphorylated p53 can down-regulate and phosphorylated p53 can up-regulate the pAPCP promoter activity involving the p53, Sp1, or E-box B elements. These studies are important to understanding the role of p53 and APC in DNA damage-induced cell cycle arrest and/or apoptosis of cancer cells.
Assuntos
Neoplasias do Colo/genética , Genes APC , Genes p53 , Nitrosoguanidinas/farmacologia , Alquilantes/farmacologia , Apoptose/efeitos dos fármacos , Sequência de Bases , Neoplasias do Colo/patologia , Dano ao DNA/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Dados de Sequência Molecular , Transcrição Gênica/efeitos dos fármacos , Células Tumorais CultivadasRESUMO
The adenomatous polyposis coli (APC) gene product is involved in cell cycle arrest and apoptosis, and loss of function is associated with the development of colorectal carcinogenesis. Although it has been demonstrated that the APC gene is inducible, its transcriptional regulation has not been elucidated. Therefore, we characterized the promoter region of the APC gene and transcription factors required for basal expression. The APC gene has a TATA-less promoter and contains consensus binding sites for Octamer, AP2, Sp1, a CAAT-box, and three nucleotide sequences for E-box A, B, and M. The E-boxes are functional in several cancer cell lines and upstream stimulating factor-1 (USF1) and USF2 interact with these sites, with a preferred sequence-specificity for the B site. Analysis of activation of the cloned APC promoter by USF1 and USF2 in transient transfection assays in HCT-116 cells demonstrated that mutation of the E-box B site completely abolished the basal promoter activity. Further, the ectopic USF1 and USF2 expression in HCT-116 cells with deletion mutations of E-box A, B, and M sites showed that these E-boxes contribute to USF1- and USF2-mediated transcriptional activation of the APC promoter, with maximum promoter activity being associated with the E-box B site. Thus, USF1 and USF2 transcription factors are critical for APC gene expression.
Assuntos
Proteínas do Citoesqueleto/genética , Proteínas do Citoesqueleto/metabolismo , Proteínas de Ligação a DNA , Fatores de Transcrição/metabolismo , Proteína da Polipose Adenomatosa do Colo , Apoptose , Sequência de Bases , Sítios de Ligação , Linhagem Celular , Cloranfenicol O-Acetiltransferase/metabolismo , Clonagem Molecular , Neoplasias do Colo/metabolismo , Deleção de Genes , Humanos , Mutação , Plasmídeos/metabolismo , Regiões Promotoras Genéticas , Transcrição Gênica , Ativação Transcricional , Transfecção , Células Tumorais Cultivadas , Raios Ultravioleta , Fatores Estimuladores UpstreamRESUMO
Feeding of protein deficient diet is known to alter the transmembrane signalling in brain of rat by reducing total protein kinase C (PKC) activity. Phospholipid metabolism regulates the activation of PKC through generation of second messengers and the extent of PKC activation accordingly influences the magnitude of phosphorylation of its endogenous substrate proteins. Thus it was speculated that ingestion of protein deficient diet may modify the turnover rate of membrane phospholipids and magnitude of phosphorylation of endogenous substrate proteins of PKC. The experiments were conducted on rats fed on three different types of laboratory prepared diets viz. casein (20% casein), deficient (4% protein, rice flour as source of protein) and supplemented (deficient diet supplemented with L-lysine and DL-threonine) for 28 days. The metabolism of phosphoinositides (PIs) and phosphatidyl choline (PC) was studied by equilibrium labeling with [3H] myo inositol and [14C methyl] choline chloride respectively. The phosphorylation of endogenous substrate proteins of PKC was studied by using 32P-gamma-ATP followed by SDS-PAGE and autoradiography. The results suggest that in deficient group, there is an increased incorporation of [3H] myo inositol in PIs and inositol phosphate pool in comparison to the casein group. The phosphatidyl inositol (PI) turnover reduced, although there was a marginal increase in the phosphatidyl inositol monophosphate (PIP) and phosphatidyl inositol bis phosphate (PIP2). Supplementation of diet showed a reversal of the pattern towards control to a considerable extent. In the deficient group, PC metabolism showed an increased incorporation of [14C methyl] choline in choline phospholipids but decreased incorporation in phosphoryl choline in comparison with the casein group. The increase in total PC contents was significant but marginal in residue contents. The turnover rate of PC increased only marginally and that of residue declined. Supplementation of diet reduced the total contents of PC and residue, but the turnover rate of PC and residue remained still higher. Phosphorylation of endogenous proteins showed four different proteins of 78, 46, 33 and 16 kDa to be the substrates of PKC in casein group. In deficient group, phosphorylation of these proteins increased markedly while supplementation of diet had a reversing effect rendering the values to be intermediate between casein and the supplemented group. The changes in phospholipid metabolism and in phosphorylation of endogenous substrate proteins of PKC suggest that dietary protein deficiency causes alterations in transmembrane signalling mechanism in rat brain. These effects are partially reversed by improving the quality of proteins in the diet.
Assuntos
Encéfalo/metabolismo , Dieta com Restrição de Proteínas/efeitos adversos , Fosfolipídeos/metabolismo , Animais , Masculino , Lipídeos de Membrana/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Fosforilação , Proteína Quinase C/metabolismo , Ratos , Ratos Wistar , Transdução de SinaisRESUMO
One of the functions of adenomatous polyposis coli (APC) in colorectal cancers is regulation of c-myc gene expression. However, the role of APC in lung cancers has not been elucidated. In the present study, the levels of APC and c-myc mRNA were determined in one strain of normal human bronchial epithelial (NHBE) cells, an SV-40-immortalized non-tumorigenic human bronchial epithelial cell line (BEAS-2B), 13 non-small cell lung cancer cell lines, and 4 small cell lung cancer cell lines. To establish a relationship between c-Myc and APC, we determined the ratio of c-myc and APC mRNA levels in different lung cancer cell lines. Out of 19 lung cancer cell lines, we found that 13 exhibited c-myc/APC mRNA ratio of more than two. Among the cell lines CaLu-3, NCI-H82, A427 and SW900 showed a very low level of APC mRNA and a high level of c-myc mRNA. The ratio of c-myc/APC mRNA in these cell lines was 48, 127, 325 and 708, respectively. The results of these analyses revealed an inverse relationship between APC and c-myc mRNA levels, suggesting that APC may regulate c-myc expression in lung cancer cells in a manner analogous to its role in colon cancer.
Assuntos
Proteínas do Citoesqueleto/genética , Neoplasias Pulmonares/genética , Proteínas Proto-Oncogênicas c-myc/genética , RNA Mensageiro/análise , Proteína da Polipose Adenomatosa do Colo , Proteínas do Citoesqueleto/biossíntese , Humanos , Neoplasias Pulmonares/metabolismo , Proteínas de Neoplasias/biossíntese , Proteínas de Neoplasias/genética , Proteínas Proto-Oncogênicas c-myc/biossíntese , RNA Mensageiro/genética , Células Tumorais CultivadasRESUMO
We examined the effect of a protein synthesis inhibitor, cycloheximide (CHX) on the adenomatous polyposis coli (APC) mRNA levels in HCT-116 colon cancer cell line. The HCT-116 cells were treated with different concentrations of CHX for 15 h. APC, p53 and beta-actin mRNA levels were determined by Northern blotting. Results showed that APC and beta-actin mRNA levels were significantly increased in a dose-dependent manner after CHX treatment. The p53 mRNA levels were moderately increased. The increase in APC mRNA levels after CHX treatment was due to increase in its stability instead of transcription. These results provide a model for CHX-induced APC mRNA stabilization and its implication in cell cycle arrest and cell survival studies.
Assuntos
Neoplasias do Colo/metabolismo , Cicloeximida/farmacologia , Proteínas do Citoesqueleto/biossíntese , Proteínas de Neoplasias/biossíntese , Inibidores da Síntese de Proteínas/farmacologia , RNA Mensageiro/metabolismo , Proteína da Polipose Adenomatosa do Colo , Neoplasias do Colo/genética , Proteínas do Citoesqueleto/antagonistas & inibidores , Proteínas do Citoesqueleto/genética , Relação Dose-Resposta a Droga , Regulação Neoplásica da Expressão Gênica , Genes APC/efeitos dos fármacos , Humanos , Proteínas de Neoplasias/antagonistas & inibidores , Proteínas de Neoplasias/genética , RNA Mensageiro/antagonistas & inibidores , Células Tumorais CultivadasRESUMO
In the present study, we show that treatment of wild-type (p53+/+) mouse embryonic fibroblast (MEF) cells with a DNA-alkylating agent, N-methyl-N'-nitro-N-nitro-soguanidine (MNNG), resulted in increased levels of adenomatous polyposis coli (APC) mRNA compared to p53 gene-knocked out (p53-/-) MEF cells, indicating that p53 is required for APC expression after alkylation damage. By using HCT-116 colon cancer cells (containing wild-type p53 gene) or p53-/- MEF cells transfected with a pCMV-p53 overexpression plasmid [p53-/-(CMV-p53)], we show that p53 is a labile factor for APC gene expression, and that pretreating HCT-116 cells with a protein synthesis inhibitor, cycloheximide (CHX), inhibited MNNG-induced APC mRNA levels by inhibiting p53 protein synthesis. The effect of CHX on p53 protein synthesis was reversible, as the withdrawal of CHX permitted p53 protein synthesis to resume with a concomitant increase in APC mRNA levels after MNNG treatment. To examine whether p53 regulates APC gene expression at the transcriptional level, we treated HCT-116 or p53-/-(CMV-p53) MEF cells with 5,6-dichloro-1-beta-D-ribofuranosylbenzamidazole (DRB; a transcriptional inhibitor), before the MNNG treatment. Although treatment of cells with DRB resulted in increased p53 protein levels, that the APC mRNA levels were decreased suggests that p53 may enhance APC gene expression upstream of the transcriptional machinery where DRB interacts. That the withdrawal of DRB, and subsequent MNNG treatment, increased the level of APC mRNA indicated that the binding of DRB to the transcriptional machinery was reversible.
Assuntos
Genes APC/genética , Metilnitronitrosoguanidina/farmacologia , Inibidores da Síntese de Ácido Nucleico/farmacologia , Inibidores da Síntese de Proteínas/farmacologia , RNA Mensageiro/efeitos dos fármacos , Proteína Supressora de Tumor p53/fisiologia , Animais , Carcinógenos/farmacologia , Linhagem Celular , Cicloeximida/farmacologia , Diclororribofuranosilbenzimidazol/farmacologia , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Camundongos , RNA Mensageiro/metabolismo , Células Tumorais Cultivadas/citologia , Células Tumorais Cultivadas/efeitos dos fármacos , Células Tumorais Cultivadas/metabolismo , Proteína Supressora de Tumor p53/genéticaRESUMO
Asthma is an inflammatory disease of the airways. Activation of lymphocytes leads to elaboration of inflammatory mediators which are likely to initiate and perpetuate the asthmatic response. During activation of lymphocytes, the role of protein kinase C (PKC) has been emphasized. Therefore, changes in PKC activity in peripheral blood lymphocytes in bronchial asthma can be expected, which may be due to alterations in the regulatory mechanisms of the enzyme molecule. To understand the mechanism of regulation of PKC activity the effects of drugs, such as carbachol, histamine, sphingosine and disodium cromoglycate (DSCG), on the lymphocytes of healthy subjects were studied. The study included 27 asthmatic patients and 14 healthy volunteers. Disease was classified as mild, moderate-to-severe, and cases in remission. PKC activity was determined in peripheral blood lymphocytes by [3H] phorbol 12,13-dibutyrate ([3H]PDBu)-binding assay. A highly significant (p < 0.001) increase was observed in total, cytosolic and membrane PKC activity in all of the asthmatic patients as compared to the healthy group. There was an increased translocation of PKC from cytosol to membrane in all of the groups, and the extent of translocation of the enzyme indicates physiological activation of the cells. A highly significant (p < 0.001) reciprocal relationship (r = -0.47) existed between forced expiratory volume in one second (FEV1) as percentage predicted and total PKC activity in bronchial asthma. Carbachol and histamine significantly (p < 0.001) increased PKC activity in lymphocytes, the increase being dose-dependent for histamine. Sphingosine or disodium cromoglycate (DSCG) brought about complete inhibition of PKC activity at 100 nM. We conclude that protein kinase C activity is increased in lymphocytes in bronchial asthma. Our findings suggest that the mediators (carbachol and histamine), and drugs (sphingosine and disodium cromoglycate) possibly exert their action on protein kinase C by influencing the regulatory domain of the enzyme.
Assuntos
Asma/fisiopatologia , Linfócitos/enzimologia , Proteína Quinase C/fisiologia , Adolescente , Adulto , Idoso , Asma/enzimologia , Asma/imunologia , Carbacol/farmacologia , Fracionamento Celular , Membrana Celular/enzimologia , Cromolina Sódica/farmacologia , Citosol/enzimologia , Relação Dose-Resposta a Droga , Ativação Enzimática/efeitos dos fármacos , Feminino , Volume Expiratório Forçado , Histamina/farmacologia , Humanos , Técnicas In Vitro , Ativação Linfocitária/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade , Proteína Quinase C/metabolismo , Esfingosina/farmacologiaRESUMO
Development of colon cancer is a multistep process frequently involving mutations in both the APC and p53 tumor suppressor genes. In this study we treated the HCT-116 colon cancer cell line with alkylating agents including N-methyl-N'-nitro-N-nitrosoguanidine (MNNG),which is known to cause colon cancer in animals, and examined the expression of both APC and p53 genes. Exposure of cells with MNNG caused an 8-12-fold increase in the level of APC mRNA and protein. APC induction was shown to result from increased nuclear transcription of the APC gene and correlated with a concomitant increase in the p53 protein level after MNNG treatment. A necessary role for p53 in APC gene regulation is supported by the failure of MNNG to induce APC expression in cell lines either expressing very low levels of p53 (HeLa cells) or no p53 (K562 erythroleukemia cells). The overexpression of wild-type p53 gene into HCT-116 cells mimicked the effect of MNNG-induced expression of APC mRNA. A direct causal role for p53 in APC gene regulation was further evaluated by transfecting the wild-type p53 gene into K562 cells and observing a 5-fold increase in the APC gene expression. These results support a model featuring a direct link between p53 and APC in response to alkylation-induced DNA damage and suggest a novel role for p53 in a stress-response pathway involving APC.
Assuntos
Carcinógenos/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Genes APC/genética , Metilnitronitrosoguanidina/farmacologia , Proteína Supressora de Tumor p53/metabolismo , Dano ao DNA , Células HeLa , Humanos , RNA Mensageiro/metabolismo , Transcrição Gênica/efeitos dos fármacos , Células Tumorais CultivadasRESUMO
Dietary protein deficiency is known to alter the protein kinase C activity in various tissues of rats. Protein kinase C activity is influenced by the metabolism of membrane phosphoinositides and phosphatidyl choline (PC). For metabolic studies, hepatocytes have been the cells of choice of various workers. Therefore, studies on protein kinase C and these phospholipids were conducted in hepatocytes of rats maintained on three different diets viz. casein (20% protein) deficient (4% protein, rice flour as source of protein) and supplemented (deficient diet supplemented with L-lysine and DL-threonine) diet for 28 days. The protein deficiency in diet led to a decline in protein kinase C activity (P < 0.01) without effecting its translocation, an increase in phosphatidyl inositol 4,5-bisphosphate (P < 0.001) and a decrease in phosphatidyl inositol 4-monophosphate and phosphatidyl inositol (P < 0.01) but did not alter the PC contents, as compared to the casein group. Supplementation of deficient diet with L-lysine and DL-threonine could considerably reverse the effect of deficiency of protein in diet. The results suggest that quality of dietary protein is mainly relevant for maintaining phospholipid metabolism and physiology of hepatocytes and thus the signalling mechanism in these cells.
Assuntos
Fígado/metabolismo , Fosfolipídeos/metabolismo , Deficiência de Proteína , Proteína Quinase C/metabolismo , Animais , Fígado/citologia , Masculino , Ratos , Ratos WistarRESUMO
The characteristics of protein kinase C activity present in guinea pig alveolar and peritoneal macrophages have been compared and examined. The activity is predominantly cytosolic with preference for phosphatidyl serine as cofactor over other phospholipids. K(m) of protein kinase C for ATP is 30.30 and 54.05 microM in alveolar and peritoneal macrophages respectively. Scatchard plot analysis shows heterogenous binding sites for [3H]PDBu in alveolar macrophages in contrast to peritoneal macrophages showing homogeneous type of binding sites. PMA activates protein kinase C in a dose-dependent manner and shows downregulation at higher concentration in both alveolar and peritoneal macrophages. Endogenous proteins of molecular masses 77, 47, 37 and 16.5 kDa in alveolar macrophages and 77, 47, 38 and 15.5 kDa in pertioneal macrophages are phosphorylated by PKC. These findings suggest that alveolar and peritoneal macrophages possess two different types of protein kinase C activities but phosphorylate similar proteins and exhibit functional similarities in these cells.
Assuntos
Macrófagos Alveolares/enzimologia , Macrófagos Peritoneais/enzimologia , Proteína Quinase C/metabolismo , Animais , Comunicação Celular/fisiologia , Ativação Enzimática , Cobaias , Macrófagos Alveolares/citologia , Macrófagos Peritoneais/citologiaRESUMO
Ingestion of protein deficient diet is known to decrease the enzyme load, particularly drug metabolising enzymes in liver. It also leads to decrease in polyphosphoinositide pool in brain and kidney. Therefore, changes in protein kinase C activity and its translocation were speculated and studied in brain, lung, heart, spleen, liver and kidney of rats maintained on three different diets, viz. casein (20% protein) deficient (4% protein, rice flour as protein source) and supplemented (deficient diet supplemented with L-lysine and DL-threonine), for 28 days. A significant alteration in total protein kinase C activity and/or its translocation was observed in these tissues in the deficient group in comparison to casein group. Supplementation of diet with L-lysine and DL-threonine could partially reverse the affect. These changes in protein kinase C activity and its translocation indicate alteration in the mechanism of signalling system in dietary protein deficiency and hence an altered response of tissues to the external stimuli in dietary protein deficiency.
Assuntos
Proteínas Alimentares/farmacologia , Proteína Quinase C/metabolismo , Animais , Transporte Biológico/fisiologia , Masculino , Especificidade de Órgãos , Ratos , Ratos WistarRESUMO
Phospholipid composition of sheep blood platelets and its various plasma membrane fractions have been analyzed. Based on their flotation rates in discontinuous sucrose density gradient centrifugation, three membrane fractions were isolated. 5'-Nucelotidase and alkaline phosphatase were distributed nearly equally in all the three membrane fractions. However these membrane fractions showed differences in the distribution of phosphatidyl ethanolamine, phosphatidyl choline and phosphoinositides. Phosphatidyl ethanolamine was predominant in fraction I (11.05 micrograms PLP/mg protein) while phosphatidyl choline was predominant in fractions II and III (110.10 and 68.30 micrograms PLP/mg protein respectively). Phosphatidyl inositol (Ptd-InsP) was equally distributed in all three membrane fractions. However, both Ptd-InsP and phosphatidyl inositol 4,5-bisphosphate were about 4-fold higher in fraction II (73.55 and 89.89 micrograms PLP/mg protein respectively).
Assuntos
Plaquetas/química , Lipídeos de Membrana/sangue , Fosfatidilinositóis/sangue , Fosfolipídeos/sangue , Animais , Plaquetas/ultraestrutura , Fracionamento Celular/métodos , Membrana Celular/química , Membrana Celular/ultraestrutura , Centrifugação com Gradiente de Concentração/métodos , Lipídeos de Membrana/isolamento & purificação , Fosfatidilinositóis/isolamento & purificação , Fosfolipídeos/isolamento & purificação , OvinosRESUMO
Effect of feeding rice diet with and without lysine and threonine supplementation on hepatic mitochondria and its inner and outer membrane proteins, enzymes and phospholipids has been studied. The exchange of phosphatidylcholine and phosphatidylethanolamine between microsomes and mitochondria has also been studied under these conditions. Deficient diet lead to significant decrease in proteins as well as activities of monoamine oxidase, succinate dehydrogenase, cytochrome a + a3 and cytochrome c in mitochondria and its inner and outer membranes. Feeding of the deficient diet also significantly reduced total phospholipids and PC in mitochondria and its outer mitochondrial membrane. In the inner mitochondrial membrane, only PE and cardiolipin were reduced. The incorporation (DPM/microgram PLP) of [methyl-3H]choline and [methyl-14C]methionine into PC of mitochondria and its outer membrane and that of 32Pi into PC and PE of outer mitochondrial membrane but only into PC of inner mitochondrial membrane were significantly reduced in the deficient group. The exchange rates of PC and PE between microsomes and mitochondria were reduced in the deficient group. Supplementation of the deficient diet with lysine and threonine profoundly improved the above biochemical lesions as compared to casein fed rats.