RESUMO
This research aims to characterize efficiency of a flexible ureteroscope that is of single use with regard to surgical time, absence of stone, and complications. From March 2022 to April 2023, the Basrah Urological Centre carried out this anticipated work. After excluding patients with untreated urinary tract infections, excessive blood urea, and ureteral strictures, the study involved ninety-eight patients. All patients were above 20 years of age. Patients were operated on by the same surgeon. This study involved 108 patients in this study composed of 42 (39.8%) men and 65 (60.2%) women. With a standard deviation of 10.9 years, the patient's mean age was 39.2 years. The total stone burden ranged from 6.9 to 14.5 mm, averaging 9.7±2.9 mm. The stone density ranged from 820-1411 HU, averaging 1000.8±279.3 HU. According to the current study, treating renal stones with a single-use flexible ureteroscope is less complicated and more successful.
Assuntos
Cálculos Renais , Litotripsia , Ureteroscópios , Humanos , Feminino , Masculino , Adulto , Cálculos Renais/cirurgia , Cálculos Renais/terapia , Litotripsia/instrumentação , Litotripsia/métodos , Pessoa de Meia-Idade , Equipamentos Descartáveis , Ureteroscopia/instrumentação , Ureteroscopia/métodos , Duração da CirurgiaRESUMO
Ovine pulmonary carcinoma (OPC) is a contagious neoplasm of alveolar epithelial type II (ATII) or Clara cells caused by a type D/B chimeric retrovirus, jaagsiekte sheep retrovirus (JSRV). Here we report the isolation, sequencing, pathogenicity, and integration site of a JSRV provirus isolated from a sheep lung tumor cell line (JS7). The sequence of the virus was 93 to 99% identical to other JSRV isolates and contained all of the expected open reading frames. To produce virions and test its infectivity, the JS7 provirus (JSRV(JS7)) was cloned into a plasmid containing a cytomegalovirus promoter and transfected into 293T cells. After intratracheal inoculation with virions from concentrated supernatant fluid, JSRV-associated OPC lesions were found in one of four lambs, confirming that JSRV(JS7) is pathogenic. In JS7-cell DNA, the viral genome was inserted in the protein-coding region for the surfactant protein A (SP-A) gene, which is highly expressed in ATII cells, in an orientation opposite to the direction of transcription of the SP-A gene. No significant transcription was detected from either the viral or the SP-A gene promoter in the JS7 cell line at passage level 170. The oncogenic significance of the JSRV proviral insertion involving the SP-A locus in the JS7 tumor cell line is unknown.
Assuntos
Retrovirus Jaagsiekte de Ovinos/genética , Proteolipídeos/genética , Provírus/genética , Adenomatose Pulmonar Ovina/virologia , Surfactantes Pulmonares/genética , Integração Viral/genética , Animais , Sequência de Bases , Sítios de Ligação , Linhagem Celular Transformada , DNA Viral , Humanos , Retrovirus Jaagsiekte de Ovinos/isolamento & purificação , Retrovirus Jaagsiekte de Ovinos/patogenicidade , Neoplasias Pulmonares , Dados de Sequência Molecular , Provírus/isolamento & purificação , Proteína A Associada a Surfactante Pulmonar , Proteínas Associadas a Surfactantes Pulmonares , Ovinos , Células Tumorais CultivadasRESUMO
An efficient and reproducible technique is described for the isolation of transformed sheep pulmonary adenomatosis cells. It includes three basic steps: prolonged trypsinisation to kill fibroblasts, magnetic removal of macrophages and adherence to remove the rapidly adherent cells. The resultant preparations of lung cells were enriched to 96.6 per cent type 2 pneumocytes.